Implantation outcome is Associated with Mucin-16, MiR-1226 and MiR-210 Expression during IVF of Iraqi Females Israa M.Majeed, Asmaa M. Salih Almohaidi Iraqi Journal of Pharmaceutical Sciences, 2025 One of the main obstacles to early pregnancy and assisted reproduction is embryo implantation failure. Endometrial receptivity and the interactions between the embryo and the mother determine implantation. The control of implantation involves several molecules. One such molecule is microRNA (miRNA), which is known to have a role in embryo implantation as a transcriptional regulator of gene expression. The current study evaluates the Expression level of miR-210 and miR-1226 with mucin-16 in infertile females under the IVF program and its influence on embryo implantation by measuring the fold change. This study included 26 successful implantations and 58 failed implantations under an in vitro fertilization program and 44 fertile females as control divided into two subgroups, 21 successful implantations and 23 failed implantations. The first conclusion in the current work is that the infertility cases under the IVF program with fertile failure implantation cases related to significant up-regulation of mucin-16, except successful fertile cases, recorded significant down-regulation. There is a significant positive correlation between mucin16 and miR-210-HG in failure embryo implantation in infertile females and a negative correlation between mucin16 with miR-210-HG and miR-1226 in failure embryo implantation in fertile females. These results recorded that up-regulation of miR-210 and miR-1226 gene expression may affect embryo implantation by regulating mucin-16 gene expression in infertile and fertile females undergoing IVF programs.
The Association Between rs2414096 of CYP19 Gene Polymorphism and Polycystic Ovary Syndrome (PCOS) in Iraqi Women Asmaa M. Salih Almohaidi, Mohanad W. Mahdi Alzubaidy, Luay Qasim Abdulhameed, Mohammed Bouskout, Aseel J. Kadim, Arshad Mohammed Nafie Journal of Obstetrics Gynecology and Cancer Research, 2025 Background & Objective: Women of reproductive age suffer from Polycystic Ovarian Syndrome (PCOS). The present study was conducted to investigate the association between the SNP rs2414096 in the cytochrome P450 CYP19 gene and polycystic ovarian syndrome in Iraqi women.Materials & Methods: Blood samples were collected from Iraqi women with PCOS at the Maternity Teaching Hospital (Al-Batool) in Baquba city, Diyala Governorate, Iraq. Participants' ages ranged from 29 to 57 years. Genotyping was performed using PCR-based techniques with specific primers, and the association between genotypes and PCOS risk was assessed through Hardy-Weinberg Equilibrium (HWE). Data analysis was done using statistical tests. P<0.05 was considered statistically significant.Results: HWE analysis revealed no significant differences between the observed and expected genotype frequencies in both PCOS patients and healthy controls (P>0.8885, P>0.6377, respectively). A comparative analysis of genotype and allele frequencies showed no significant differences between the groups. The GA and AA genotypes of rs2414096 were more prevalent in both patients and healthy individuals and may be protective against PCOS (RR=0.88, 0.52). In contrast, the GG genotype was associated with an increased risk of PCOS (RR=2.32). Allele analysis suggested that the A allele may have a protective role, while the G allele may contribute to the development of PCOS.Conclusion: The SNP rs2414096 in the CYP19 gene may not significantly impact the development of PCOS in Iraqi women. Further studies of larger sample sizes are needed to confirm these findings
Mucin4 (MUC4) Gene and Pri–miR146a Expression in Iraqi Females under In Vitro Fertilization Program Israa M. Majeed, Asmaa M. Salih Almohaidi Iraqi Journal of Science, 2025 MUC4, a type of reproductive mucus secreted by epithelial cells, can alter the uterine environment and weaken the epithelial lining, making it challenging for an embryo to implant. Furthermore, MUC4 has been linked to mature endometriosis, a condition that affects the uterus. As a result, abnormal mucus production led to female Infertility. The current study evaluates the association between mucin4, pri-miR146a, with Infertility by measuring the fold change in gene expression to improve IVF program in Iraqi medical centres. This was achieved by assessing a new parameter in 128 infertility women under IVF. The study included 84 infertile females: - 26 successful implantations, 58 failure failed implantations under an in vitro fertilization program and 44 fertile females under IVF as control divided into two subgroups, 21 successful implantations and 23 failed implantations. Current work conclusion, the infertility, cases under the IVF program with fertile failure implantation cases related to significant down-regulation of mucin-4, except successful fertile cases recorded significant up-regulation. These results recorded up-regulation of miR-146a-5p gene expression and down-regulation of miR146a-3p expression. There is a significant negative correlation between mucin4 with miR-146a-5p and miR-146a-3p. These relations may influence embryo implantation by regulating mucin4 gene expression under the effect of negative correlation of both 5p and 3p in infertile females undergoing IVF programs.
Polymorphism of Genes in Iraqi Females with Type 2 Diabetes Mellitus Almohaidi, Asmaa M. Salih, Saeed, Rana Hadi, Mirza, Seemaa Fahad, Mohammed, Walaa A., Hassan, Ibtisam Badday Proceedings of International Conference on Applied Innovation in IT, 2025 Diabetes Mellitus Type 2 (T2DM) is a global medical challenge, with cytokines playing a significant role in its developing. This study evaluates the frequency of polymorphisms in Interleukin-1α (IL-1α) at position -889 C/T (rs1800587), Interleukin-1β (IL-1β) at positions -511 C/T (rs16944) and +3962 C/T (rs1143634) and compared to healthy controls, diabetic patients exhibited an increased risk associated with the T allele of IL-1RL1-27609. In the study, which was conducted by Al-Yarmouk Teaching Hospital, 720 newly diagnosed Iraqi females with T2DM, aged 20-55 years, were included along with 240 age- and gender-matched healthy controls recruited from their own hospital. Genotyping was performed by using 49 patients and 40 normal after excluded others diseases and conducted the PCR-SSP assay, and results were validated by sequencing. Allele and genotype frequencies were analysed, and significant associations were identified. The findings revealed that the T allele and TT genotype of IL-1α (-889 C/T) significantly increased the risk of T2DM, while the C allele and CC genotype were protective. For IL-1β-511 C/T, the T allele acted as a protective factor, whereas the C allele elevated the risk of diabetes. No significant associations were observed for IL-1β +3962 C/T. Interestingly, polymorphisms in the IL-1RL1 receptor -27609 T/C showed that the T allele was related with increased susceptibility to diabetes, whereas the C allele was protective. Polymorphisms in IL-1α-889 C/T, IL-1β -511 C/T, and IL-1RL1 -27609 T/C appear to contribute to the genetic susceptibility to T2DM in Iraqi females. These findings highlight the potential role of interleukin signalling pathways in diabetes development. Further studies with larger, more diverse populations are recommended to confirm these results and explore their clinical implications.
Integrin Alpha2 and Integrin Beta3 Polymorphisms are Risk Factors for Infertility in Iraqi Infertile Females under In vitro Fertilization Program Asmaa M. Salih Almohaidi, Rabab A.Yousif Baghdad Science Journal, 2025 صُممت الدراسة الحالية للتحري عن تأثير تعدد الطرز أحادي النوكليوتيدات (SNP) ، للتتابع المرجعي 5918 ،(T> C) لمورث إنتيجرين بيتا 3 (ITGB3) وللتابع المرجعي 1801106 (G> A) إنتجرين ألفا 2 (ITGB3) في عينات دم اناث عراقيات. 71 أنثى مصابة بالعقم خاضعة لبروتوكول أطفال الأنابيب (مقسمة إلى مجموعتين فرعيتين ، 29 عملية زرع ناجحة و 42 عملية زرع فاشلة) و 50 أنثى خصبة كمجموعة سيطرة. تم الحصول على عينات الدم من المرضى ومجموعات السيطرة في المستشفيات العامة في بغداد ، العراق من مارس 2021 إلى أبريل 2021. تم إجراء التنميط الوراثي لتعدد الطرز باستخدام تحليل الذوبان عالي الدقة في الوقت الحقيقي (HRM في الوقت الحقيقي PCR) من الحمض النووي الرايبوزي منقوص الاوكسجين الجينومي المنقى المأخوذ من عينات الدم. أظهرت النتائج أن الطرز الوراثية TT و GG لـلتتابعات المرجعية 5918 في ITGB3 و 1801106 في ITGA2 على التوالي ، كانت أعلى بشكل ملحوظ في الإناث الخصبة من الاناث العقيمات. يشير ذلك إلى أن الطراز الوراثي GG يعمل كعامل وقائي ، بينما يعمل الطراز الوراثي TC لـلتتابع المرجعي 5918 في ITGB3 و AA من الطراز الوراثي للتتابع المرجعي 1801106 في ITGA2 كعوامل خطر. الاستنتاج ، ان لتعدد الطرز في جينات ITGA2 و ITGB3 تأثير على استعداد مجموعة من الاناث ضمن الدر اسة للإصابة بالعقم.
Endothelin-1 Gene Polymorphism with Interleukin-1β Expression in Infertile Iraqi Women under In Vitro Fertilization Program Zainab Jalil Abdulkareem, Asmaa M. Salih Almohaidi Baghdad Science Journal, 2025 تقبل بطانة الرحم هي المرحلة المحددة لنجاح العلاج ببرنامج أطفال الانابيب، يتم تنظيم غزو خلايا الأرومة الغاذية البشرية لبطانة الرحم من خلال العديد من العوامل مثل EDN1 و IL-1β. تهدف هذه الدراسة الى التحقق من العلاقة بين تعدد الاشكال والتعبير الجيني لجينات EDN1 و IL-1β وانغراس الجنين في النساء المصابات بالعقم الخاضعات لبرنامج الاخصاب خارج الرحم. تم جمع عينات الدم المحيطي في يوم الارجاع بعد ساعة من ارجاع الجنين. بعد اخذ الموافقة من 60 امرأة وتم تقسيمها الى مجموعتين وفقا لنتيجة انغراس الجنين، مجموعة فشل لديهن الانغراس شملت 35 امرأة، ومجوعة نجح لديهن الانغراس شملت 25 امرأة. كشفت نتائج تتابع تسلسل الحمض النووي عن وجود تغايرين وراثيين في جين EDN1، rs2070699 لم يظهر أي ارتباط بنتيجة انغراس الجنين في النساء المصابات بالعقم وفقا لاحتمالية فيشر، واظهر تواترا عاليا في مجموعتي الدراسة للطرز الوراثية GG و TT مقارنة بالطراز الوراثي GT. ويظهر التغاير No.7196T>G على الإكسون الثاني ان الطراز الوراثي TT كان الأعلى تكرارا في مجموعة النجاح، بينما الطراز الوراثي TG هو الأعلى تكرارا في مجموعة الفشل. نتائج التعبير الجيني لجين EDN1 و IL-1β لم تظهر أي اختلاف معنوي بين مجموعتي الدراسة، واظهر الارتباط بين EDN1 و IL-1β علاقة إيجابية معنوية. نستنتج، ان التغايرات الوراثية rs2070699 و No.7196T>G ليس لها تأثير على مستوى التعبير الجيني عند النساء المصابات بالعقم الخاضعات لبرنامج أطفال الانابيب. التعبير الجيني لجين EDN1 و IL-1β يمتلكان علاقة إيجابية مهمة في المرحلة الأصفرية وكلاهما يزداد التعبير عنه في النساء اللواتي لديهن انغراس جنيني ناجح.
Effect of Green Selenium Nanosynthesis on Staphylococcus Aureus Ali, Hassan Naser, Almohaidi, Asmaa M. Salih, Alzubadiy, Mohanad W. Mahdi Proceedings of International Conference on Applied Innovation in IT, 2025 Selenium nanoparticles (SeNPs) represent a promising natural compound for modern medical treatments, offering a potent new weapon against pathogenic bacteria. This study aimed to synthesize SeNPs using sodium selenite (Na₂SeO₃) as a precursor and dried Camellia sinensis (green tea) leaf extract as a reducing and stabilizing agent through an eco-friendly biosynthesis approach. The synthesis was performed via a simple, cost-effective, and environmentally friendly method. The resulting nanoparticles were characterized using UV-visible spectroscopy, field-emission scanning electron microscopy (FE-SEM), and energy-dispersive X-ray spectroscopy (EDX). FE-SEM analysis confirmed the formation of spherical SeNPs with varied shapes and an average particle size of 20.5 nm, alongside a narrow size distribution. While the antibacterial evaluation against Staphylococcus aureus showed no significant inhibitory activity against planktonic cells, the SeNPs exhibited a strong antibiofilm effect, inhibiting biofilm formation by 54.7%. These findings highlight the potential of biosynthesized SeNPs as a specific and effective anti-biofilm agent for targeting bacterial biofilms and combating antibiotic resistance.
Improvement rapid molecular detection of Pseudomonas aeruginosa infected some Iraqi patients and it's antimicrobial susceptibility Research Journal of Pharmaceutical Biological and Chemical Sciences, 2016
