Stefanie do Prado da Silva

@unicamp.br

Master's Degree in Agricultural Engineering - FEAGRI
State University of Campinas - UNICAMP

Stefanie do Prado da Silva


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https://researchid.co/stefaniesilva

RESEARCH, TEACHING, or OTHER INTERESTS

Agricultural and Biological Sciences, Soil Science
3

Scopus Publications

Scopus Publications

  • Exogenous ACC, ABA, and/or Ethylene Enhance Berry Color Without Reducing Postharvest Performance in ‘Benitaka’ and ‘Rubi’ Table Grapes
    Aline Cristina de Aguiar, Bianca Liriel Martins Barbosa, Danielle Mieko Sakai, Stefanie do Prado da Silva, Sergio Ruffo Roberto
    Horticulturae, 2025
    The objective of this work was to assess the association of ACC (1-aminocyclopropane-1-carboxylic acid), S-ABA (abscisic acid), and ethephon on color development and anthocyanin accumulation in berries, as well as on other quality attributes of ‘Benitaka’ and ‘Rubi’ table grapes grown in a subtropical region, in addition to postharvest conservation of clusters and vine regrowth. As a statistical model, a randomized block design consisting of nine treatments and four replications was used. The treatments included different associations of ACC, S-ABA, and ethephon, by using the commercial formulations Accede®, ProTone®, and Ethrel® containing 400 g kg−1 of ACC, 100 g L−1 of S-ABA, and 720 g L−1 of ethephon, respectively. The total anthocyanins, berry color index (CIRG), physicochemical characteristics, and cluster color coverage were assessed weekly, while berry firmness was assessed at harvest. After being harvested, the clusters were placed under cold storage at 1.0 ± 1.0 °C, and after 45 days, their postharvest attributes were assessed, as well as the vine regrowth in the following season. The exogenous and combined application of compounds at véraison was demonstrated to be a strategy to trigger the development of color in ‘Benitaka’ and ‘Rubi’ table grapes. For the ‘Benitaka’ table grape, the clusters treated with the different combinations of ACC and S-ABA, ethephon and S-ABA, or ethephon alone resulted in the highest concentration of total anthocyanins and the highest CIRG means (4.90; 4.86; 4.82; 4.81, 4.73, and 4.70 mg g−1 for anthocyanins, and 6.12, 6.08, 5.97, 5.92, 5.85, and 5.74 for CIRG, respectively). For the ‘Rubi’ table grape, the combinations of ACC and S-ABA at 7 days after véraison (DAV), or ethephon and S-ABA at 7 and 14 days, resulted in higher means of anthocyanins and CIRG (3.86, 3.51, and 3.40 mg g−1 for anthocyanins and 5.05, 4.68, 4.82, and 4.79 for CIRG, respectively). Furthermore, the firmness of the berries of both cultivars remained unchanged, and after 45 days of cold storage, no reduction in the quality of the evaluated postharvest attributes was found. It was concluded that a single application of ACC 0.20 g L−1 + S-ABA 0.250 g L−1 at 7 DAV was sufficient to promote the accumulation of anthocyanins and resulted in an intense and uniform color in the berries for both varieties assessed, with no adverse impacts on the postharvest conservation of the clusters or on the regrowth of the vines. The significance of this research was to demonstrate that table grapes with insufficient skin color can be improved through a combination of S-ABA and ACC at lower concentrations of active ingredients.
  • Application of a Novel Formulation of 1-Aminocyclopropane-1-carboxylic Acid (ACC) to Increase the Anthocyanins Concentration in Table Grape Berries
    Aline Cristina de Aguiar, Danielle Mieko Sakai, Bianca Liriel Martins Barbosa, Stefanie do Prado da Silva, Fábio Yamashita, et al.
    Plants, 2025
    The objective of this work was to assess different concentrations of a novel formulation of 1-aminocyclopropane-1-carboxylic acid (ACC) on anthocyanin accumulation and color development, as well as on the physicochemical characteristics of the ‘Benitaka’ table grape grown in a subtropical region in two application forms. The trial was conducted on a commercial property located in a subtropical area in Brazil in 2022. Treatments included different concentrations of a new formulation containing 400 g kg−1 of ACC, ranging from 0 to 125 g 100 L−1, as well as a standard concentration of a formulation containing 100 g L−1 of abscisic acid (S-ABA): 3.2 L ha−1. The exogenous application of ACC was performed at the beginning of berry ripening (véraison), while that of S-ABA was performed twice: the first, at véraison, and the second, 7 days later. The concentration of total anthocyanins, berry color index, physicochemical characteristics, and sensory–visual analysis of color coverage of the bunches were evaluated weekly, while berry firmness was appraised at harvest. A single exogenous application of ACC or two applications of S-ABA resulted in daily increment rates that provided a high accumulation of total anthocyanins, as well as greater berry color development, regardless of the application method, directed to the canopy of the vines or only to the bunches. As a result, the new formulation of ACC at concentrations of 75 g to 100 g 100 L−1 is a novel tool to stimulate the anthocyanins accumulation and berry color development in ‘Benitaka’ table grapes grown in subtropical regions without negative impact on bunches or vines.
  • Essential oil from orange peel in the control of Botrytis cinerea and in the postharvest conservation of ‘Benitaka’ table grape
    Aline Cristina de Aguiar, João Paulo de Oliveira, Marcos Letaif Gaeta, Danielle Mieko Sakai, Bianca Liriel Martins Barbosa, et al.
    Semina Ciencias Agrarias, 2024
    The objective of this work was to evaluate the efficiency of essential oil from orange peel in the refrigerated conservation of the ‘Benitaka’ table grape, as well as to evaluate its in vitro effectiveness on Botrytis cinerea, the causal agent of gray mold. Grapes were harvested from a commercial field in the municipality of Cambira, Paraná, during the 2022 and 2023 seasons. The experimental design was completely randomized, with four treatments and five replications of five bunches per plot. The treatments were: a) control; b) essential oil from orange peel at 4.0 mL of the commercial product (c.p.) L-1; c) dual phase SO2-generating pads containing 1 and 4 g of the active ingredient (a.i.) in the fast and slow phases, respectively; and d) essential oil from orange peel at 4.0 mL c.p. L-1 associated with the dual phase SO2-generating pads containing 1 and 4 g of the a.i. in the fast and slow phases, respectively. A commercial product containing 61.14 g L-1 (6% w/v) of 4-isopropenyl-1-methylcyclohexane, the source of orange essential oil, was applied by spraying it directly onto the bunches. After drying, the grape bunches were stored in a refrigerated chamber at 1.0±1°C and 95% relative humidity. The following variables were assessed 30 and 45 days after the beginning of cold storage: the incidence of gray mold on berries, loss of bunch mass, stem browning, shattered berries, and bleaching. The minimum inhibitory concentration for the development of B. cinerea was determined, and fungal mycelia were observed using scanning electron microscopy to evaluate the in vitro efficacy of orange essential oil. The data were subjected to analysis of variance, and the means were compared using Fisher's difference test at 5% probability. The effectiveness of orange essential oil in suppressing the development of B. cinerea was demonstrated both in vivo and in vitro, making it a safe alternative for the postharvest conservation of 'Benitaka' table grapes.