Helen Christine O'Neill

Scopus Publications

How well do women understand their menstrual cycles? Insights from 383,085 UK based women
Zoya Enakshi Ali, Esther Wainwright, Sugha Murugesu, Silvia Moraru, Lucinda Lawrie, Natalie Getreu, Helen C. O’Neill
Reproductive Health, 2026
Menstrual cycle awareness is a critical yet frequently overlooked component of reproductive health; it shapes an individual’s ability to recognise changes in their cycle, seek timely medical care when needed, and make informed decisions. However, many women of reproductive age lack essential understanding about their cycles, contributing to widespread misconceptions, stigma, and delays in healthcare engagement. This study explores menstrual cycle awareness, the perceptions of cycle regularity, and demographic disparities among UK-based women. This was a retrospective, cross-sectional analysis of self-reported menstrual cycle data collected through an online health assessment (OHA) between September 2020 and January 2025 from 383,085 UK-based women aged 18–50 years. Participants reported cycle regularity, cycle length, and period length, alongside demographic and reproductive characteristics. Overall, 22.2% of participants reported not knowing their cycle length, with uncertainty highest among those under 25 years (33.4%). Awareness increased with age before declining slightly after age 45. Awareness of period length was higher than cycle length awareness; however, 9.5% of participants were unable to report period length, rising to 13.8% among those under 25. Among participants who described their cycles as regular and reported a cycle length, 4.9% reported cycle lengths outside commonly referenced regular range (21–35 days), indicating discordance between perceived regularity and reported cycle length. This discordance was most pronounced among younger participants and varied modestly across ethnic groups. Participants who had previously been pregnant or were actively trying to conceive demonstrated higher cycle length awareness. These findings highlight persistent gaps in menstrual cycle awareness among UK-based women, particularly among younger individuals and those not actively engaged in fertility planning. Discordance between perceived cycle regularity and cycle length reference ranges suggests that many individuals may lack a clear framework for interpreting their menstrual patterns. Improving population-level education and clinical engagement around menstrual cycle awareness beyond fertility-focused contexts may support earlier recognition of potential menstrual health concerns and more timely healthcare engagement.
Oocyte quality in the era of AI: integration of morphology, metabolic activity and time-lapse imaging
David K Gardner, Helen C O’Neill, Başak Balaban, Marcos Meseguer, Thomas Freour, Laura Rienzi
Reproductive Biomedicine Online, 2026
Fertility awareness in 97,414 women trying to conceive: gaps, misconceptions, and implications for reproductive education
Esther Wainwright, Zoya Ali, Lucinda Lawrie, Natalie Getreu, Helen C. O’Neill
Reproductive Health, 2025
BACKGROUND: Fertility rates in the UK are at an all-time low, with infertility affecting approximately 1 in 7 couples. Despite the rising demand for fertility services, fertility awareness, specifically knowledge of ovulation and the fertile window, remains low among women of reproductive age. Most existing studies offer a broad perspective, lacking focus on women actively trying to conceive (TTC). This study aims to assess the level of understanding surrounding the fertile window among women TTC, identifying factors associated with knowledge gaps. METHODS: A retrospective, cross-sectional analysis of 97,414 women actively TTC who answered an online health assessment was conducted. Participants provided information on menstrual cycle characteristics, previous pregnancies, and fertility knowledge, including the timing of the fertile window. Frequencies, percentages were calculated and chi-squared tests performed to assess differences in categorical data. Logistic regression models were used to calculate odds ratios (ORs) to better understand factors significantly associated with not knowing the fertile window. RESULTS: Out of the total respondents (97,414), over a third (33,756, 41%) could not accurately identify the fertile window, with substantial misconceptions observed across all age groups and ethnicities. Women with previous pregnancies were more likely to correctly identify the fertile window (OR = 1.45, 97.5% CI: 1.20-1.75, p < 0.001). However, knowledge was significantly lower among those with irregular cycles, non-White ethnicities, younger age groups and longer time TTC. Additionally, misconceptions about cycle regularity were apparent, of 60,322 women describing their cycles as regular 10% did not know their cycle length (66,95) and a further 2.9% fell outside of the clinically regular 21-35 day range. These misconceptions followed a similar trend with younger age groups, non-white ethnicities and longer time TTC having significantly increased rates of misidentifying regular cycles. This further increased the odds of not knowing their fertile window (OR = 2.99, 97.5% CI: 2.83-3.17, p < 0.001). CONCLUSIONS: The findings reveal gaps in fertility awareness among women actively TTC. Addressing these knowledge gaps through targeted educational interventions could potentially reduce time-to-pregnancy and the reliance on assisted reproductive technologies. Improved fertility education focusing on cycle tracking and ovulation timing is essential to assist women with accurate information during their TTC journey.
The effect of myeloablative radiation on urinary bladder mast cells
Jessica Smith, Jonathan Kah Huat Tan, Christie Short, Helen O’Neill, Christian Moro
Scientific Reports, 2024
Radiation-induced cystitis is an inflammatory condition affecting the urinary bladder, which can develop as a side effect of abdominopelvic radiotherapy, specifically external-beam radiation therapy or myeloablative radiotherapy. A possible involvement of mast cells in the pathophysiology of radiation-induced cystitis has been indicated in cases of external-beam radiation therapy; however, there is no evidence that these findings apply to the myeloablative aetiology. As such, this study investigated potential changes to urinary bladder mast cell prevalence when exposed to myeloablative radiation. Lethally irradiated C57BL/6J mice that received donor rescue bone marrow cells exhibited an increased mast cell frequency amongst host leukocytes 1 week following irradiation. By 4 weeks, no significant difference in either frequency or cell density was observed. However mast cell diameter was smaller, and a significant increase in mast cell number in the adventitia was observed. This study highlights that mast cells constitute a significant portion of the remaining host leukocyte population following radiation exposure, with changes to mast cell distribution and decreased cell diameter four weeks following radiation-induced injury.
Role, benefits, and risks of AMH testing for non-ART related indications
Zoya Enakshi Ali, Claudia Massarotti, George Liperis, Mina Mincheva, Omar F Ammar, Julia Uraji, Antonio La Marca, Raj Mathur, Helen C O’Neill, Mariana Moura-Ramos, Juan J Fraire-Zamora
Human Reproduction, 2024
The March ESHRE Journal Club discussion focused on a study by Copp et al. (2023) on the advantages and disadvantages of AMH testing, the misinterpretation of AMH levels outside an infertility treatment, the variability of AMH levels in different populations, and the impact of misleading media portrayal of DTC AMH tests on consumers.AMH, Anti-M ullerian hormone; DTC, direct-to-consumer.
Genome-wide association analyses of ovarian cancer patients undergoing primary debulking surgery identify candidate genes for residual disease
Dhanya Ramachandran, Jonathan P. Tyrer, Stefan Kommoss, Anna DeFazio, Marjorie J. Riggan, , David Bowtell, Sian Fereday, Nadia Traficante, Jillian Hung, Penelope M. Webb, Peter A. Fasching, Diether Lambrechts, María J. García, Cristina Rodríguez-Antona, Marc T. Goodman, Francesmary Modugno, Kirsten B. Moysich, Beth Y. Karlan, Jenny Lester, Susanne K. Kjaer, Allan Jensen, Estrid Høgdall, Ellen L. Goode, William A. Cliby, Amanika Kumar, Chen Wang, Julie M. Cunningham, Stacey J. Winham, Alvaro N. Monteiro, Joellen M. Schildkraut, Daniel W. Cramer, Kathryn L. Terry, Linda Titus, Line Bjorge, Liv Cecilie Vestrheim Thomsen, , Michael Friedlander, Andreas Obermair, Peter Grant, Vanessa Beesley, Penelope Blomfield, Alison Brand, Alison Davis, Yee Leung, James Nicklin, Michael Quinn, Karen Livingstone, Helen O’Neill, Merran Williams, Tanja Pejovic, Claus K. Høgdall, Iain A. McNeish, Taymaa May, David G. Huntsman, Jacobus Pfisterer, Ulrich Canzler, Tjoung-Won Park-Simon, Willibald Schröder, Antje Belau, Lars Hanker, Philipp Harter, Jalid Sehouli, Rainer Kimmig, Nikolaus de Gregorio, Barbara Schmalfeldt, Klaus Baumann, Felix Hilpert, Alexander Burges, Boris Winterhoff, Peter Schürmann, Lisa-Marie Speith, Peter Hillemanns, Andrew Berchuck, Sharon E. Johnatty, Susan J. Ramus, Georgia Chenevix-Trench, Paul D. P. Pharoah, Thilo Dörk, Florian Heitz
Npj Genomic Medicine, 2024
Survival from ovarian cancer depends on the resection status after primary surgery. We performed genome-wide association analyses for resection status of 7705 ovarian cancer patients, including 4954 with high-grade serous carcinoma (HGSOC), to identify variants associated with residual disease. The most significant association with resection status was observed for rs72845444, upstream of MGMT, in HGSOC (p = 3.9 × 10−8). In gene-based analyses, PPP2R5C was the most strongly associated gene in HGSOC after stage adjustment. In an independent set of 378 ovarian tumours from the AGO-OVAR 11 study, variants near MGMT and PPP2R5C correlated with methylation and transcript levels, and PPP2R5C mRNA levels predicted progression-free survival in patients with residual disease. MGMT encodes a DNA repair enzyme, and PPP2R5C encodes the B56γ subunit of the PP2A tumour suppressor. Our results link heritable variation at these two loci with resection status in HGSOC.
Cytocompatibility of electrospun poly-L-lactic acid membranes for Bruch's membrane regeneration using human embryonic stem cell-derived retinal pigment epithelial cells
Naghmeh Abbasi, Helen O'Neill
Journal of Biomedical Materials Research Part A, 2024
Cell replacement therapy is under development for dry age‐related macular degeneration (AMD). A thin membrane resembling the Bruch's membrane is required to form a cell‐on‐membrane construct with retinal pigment epithelial (RPE) cells. These cells have been differentiated from human embryonic stem cells (hESCs) in vitro. A carrier membrane is required for cell implantation, which is biocompatible for cell growth and has dimensions and physical properties resembling the Bruch's membrane. Here a nanofiber electrospun poly‐L‐lactic acid (PLLA) membrane is tested for capacity to support cell growth and maturation. The requirements for laminin coating of the membrane are identified here. A porous electrospun nanofibrous PLLA membrane of ∼50 nm fiber diameter was developed as a prototype support for functional RPE cells grown as a monolayer. The need for laminin coating applied to the membrane following treatment with poly‐L‐ornithine (PLO), was identified in terms of cell growth and survival. Test membranes were compared in terms of hydrophilicity after laminin coating, mechanical properties of surface roughness and Young's modulus, porosity and ability to promote the attachment and proliferation of hESC‐RPE cells in culture for up to 8 weeks. Over this time, RPE cell proliferation, morphology, and marker and gene expression, were monitored. The functional capacity of cell monolayers was identified in terms of transepithelial electrical resistance (TEER), phagocytosis of cells, as well as expression of the cytokines, vascular endothelial growth factor (VEGF) and pigment epithelium‐derived factor (PEDF). PLLA polymer fibers are naturally hydrophobic, so their hydrophilicity was improved by pretreatment with PLO for subsequent coating with the bioactive protein laminin. They were then assessed for amount of laminin adsorbed, contact angle and uniformity of coating using scanning electron microscopy (SEM). Pretreatment with 100% PLO gave the best result over 10% PLO treatment or no treatment prior to laminin adsorption with significantly greater surface stiffness and modulus. By 6 weeks after cell plating, the coated membranes could support a mature RPE monolayer showing a dense apical microvillus structure and pigmented 3D polygonal cell morphology. After 8 weeks, PLO (100%)‐Lam coated membranes exhibited the highest cell number, cell proliferation, and RPE barrier function measured as TEER. RPE cells showed the higher levels of specific surface marker and gene expression. Microphthalmia‐associated transcription factor expression was highly upregulated indicating maturation of cells. Functionality of cells was indicated by expression of VEGF and PEDF genes as well as phagocytic capacity. In conclusion, electrospun PLLA membranes coated with PLO‐Lam have the physical and biological properties to support the distribution and migration of hESC‐RPE cells throughout the whole structure. They represent a good membrane candidate for preparation of hESC‐RPE cells as a monolayer for implantation into the subretinal space of AMD patients.
Exploring the regenerative capacity of the spleen following irradiation
Christie T. M. Short, Helen C. O’Neill, Jonathan K. H. Tan
Frontiers in Hematology, 2024
IntroductionHaematopoietic stem cell transplantation (HSCT) is commonly used to treat patients with haematological disorders. Myeloablative conditioning is an important preparation for patients receiving haematopoietic stem cells (HSC) or haematopoietic stem and progenitor cells (HSPC). While widely successful, HSCT is still associated with high rates of mortality. The recovery time between complete myeloablation and haematopoietic recovery is a large factor in the recovery rate. Successful engraftment of HSC is also directly correlated with the number of HSC niches available. This highlights the importance of the haematopoietic niche and its recovery from myeloablation as an important therapeutic target.MethodsThis murine model study specifically considers changes in spleen tissue architecture and cellular composition involving stromal and vascular cells that occur following lethal irradiation.ResultsSpleen recovered fully between 4- and 8-weeks after irradiation due to reconstitution by HSPC from bone marrow. Specific temporal changes in spleen architecture were identified, and these were linked to the cell types that constitute the white pulp, red pulp and marginal zones. Mesenchymal stromal cells returned before endothelial cells, and reticular cell types recovered more quickly in spleen following irradiation. Losses in gp38+ fibroblastic reticular cells and MAdCAM-1+ marginal reticular cells were associated with loss of the white pulp in the first 4 weeks following irradiation. White pulp was restored following recovery of supporting reticular cells.DiscussionThis study tests how spleen regeneration following a lethal dose of irradiation can be influenced by co-infusion of bone marrow HSPC together with either neonatal spleen stromal cells, or cells of the stromal STX3 line. Both the infusion of neonatal spleen stromal cells and STX3 stromal cells hastened recovery of both mesenchymal and vascular compartments. Following neonatal spleen stromal cell infusion, endothelial cells increased early, but a delay in structural reformation of distinct red and white pulp areas was found. Results from this study show that spleen regeneration can be influenced and even hastened through cellular therapy. Neonatal spleen stromal cells, co-infused together with HSPC following irradiation conditioning, represent a potential therapeutic opportunity for hastening spleen regeneration.
Research ideas matter: Guidance for research students and early career researchers
Keith Duncan, Adrian Gepp, Justin Craig, Helen O'Neill
Pacific Basin Finance Journal, 2023
This paper draws on the collective experience of the authors as researchers, supervisors and editors spanning the fields of medical science, psychology, analytics, entrepreneurship, accounting, and finance. We provide guidance about research idea formulation for novice researchers, both Higher Degree Research students (HDRs) such as PhD students, and Early Career Researchers (ECRs). Drawing on parallels with business opportunity formulation, we break down the process into three steps: generating, refining and then evaluating research ideas. We present strategies to help generate high-quality research ideas, including five key perspectives for identifying gaps in the literature that are opportunities to be addressed with novel research ideas. We also provide advice for refining research ideas, and provide guidance on evaluating and ranking those ideas. Despite the diverse background of the authorship team, there are many commonalities because of the rigorous scientific nature of the general academic research process. Helpful research tools and templates, as well as examples and suggested readings, are also presented to assist novice researchers in the challenging process of forming research ideas.
GENOME EDITING IN HUMAN REPRODUCTION
Helen C. O'Neill
Textbook of Assisted Reproductive Techniques Volume 1 Laboratory Perspectives Sixth Edition, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing is a revolutionary technology that allows for precise and efficient manipulation of DNA sequences. Perhaps the most valuable use of genome editing will be in furthering our understanding of early human development, and the first licence was given to Niakan et al. to apply CRISPR/Cas9 to human embryos to interrogate the role of OCT4 in human embryo development. As such, in a bid to advance safe and effective electroporation-mediated genome editing protocols for humans, efforts are perhaps better focused on larger mammalian embryos. Base editing is a novel CRISPR-Cas9-based genome editing technology that allows the introduction of point mutations in the DNA without generating DSBs. Base editing is a type of genome editing that allows for precise changes to a single base in the genome without making a double-stranded break (DSB) in the DNA.
Skeletal stem/progenitor cells provide the niche for extramedullary hematopoiesis in spleen
Helen C. O’Neill, Hong Kiat Lim
Frontiers in Physiology, 2023
Transiently expressed CRISPR/Cas9 induces wild-type dystrophin in vitro in DMD patient myoblasts carrying duplications
Veronica Pini, Virginie Mariot, Julie Dumonceaux, John Counsell, Helen C. O’Neill, Sarah Farmer, Francesco Conti, Francesco Muntoni
Scientific Reports, 2022
Extramedullary hematopoiesis: mesenchymal stromal cells from spleen provide an in vitro niche for myelopoiesis
Sawang Petvises, Vinson Tran, Ying-Ying Hey, Dipti Talaulikar, Terence J. O’Neill, Jonathan Tan, Helen C. O’Neill
In Vitro Cellular and Developmental Biology Animal, 2022
Role of SVEP1 in Stroma-Dependent Hematopoiesis In vitro
Vinson Tran, Helen C. O’Neill
Frontiers in Cell and Developmental Biology, 2022
Efficient differentiation of human embryonic stem cells to retinal pigment epithelium under defined conditions
Ioannis J. Limnios, Yu-Qian Chau, Stuart J. Skabo, Denver C. Surrao, Helen C. O’Neill
Stem Cell Research and Therapy, 2021
Dorsal-Ventral Differences in Retinal Structure in the Pigmented Royal College of Surgeons Model of Retinal Degeneration
Una Greferath, Mario Huynh, Andrew Ian Jobling, Kirstan Anne Vessey, Gene Venables, Denver Surrao, Helen Christine O'Neill, Ioannis J. Limnios, Erica Lucy Fletcher
Frontiers in Cellular Neuroscience, 2021
Clinical germline genome editing: When will good be good enough?
Helen C. O'Neill
Perspectives in Biology and Medicine, 2020
Reactions to the National Academies/Royal Society Report on Heritable Human Genome Editing
Misha Angrist, Rodolphe Barrangou, Françoise Baylis, Carolyn Brokowski, Gaetan Burgio, Arthur Caplan, Carolyn Riley Chapman, George M. Church, Robert Cook-Deegan, Bryan Cwik, Jennifer A. Doudna, John H. Evans, Henry T. Greely, Laura Hercher, J. Benjamin Hurlbut, Richard O. Hynes, Tetsuya Ishii, Samira Kiani, LaTasha Hoskins Lee, Guillaume Levrier, David R. Liu, Jeantine E. Lunshof, Kerry Lynn Macintosh, Debra J.H. Mathews, Eric M. Meslin, Peter H.R. Mills, Lluis Montoliu, Kiran Musunuru, Dianne Nicol, Helen O'Neill, Renzong Qiu, Robert Ranisch, Jacob S. Sherkow, Sheetal Soni, Sharon Terry, Eric Topol, Robert Williamson, Feng Zhang, Kevin Davies
Crispr Journal, 2020
Transplanted spleen stromal cells with osteogenic potential support ectopic myelopoiesis
Helen C. O’Neill, Hong K. Lim, Pravin Periasamy, Lavanya Kumarappan, Jonathan K. H. Tan, Terence J. O’Neill
Plos One, 2019
Improved cryopreservation of spermatozoa using vitrification: comparison of cryoprotectants and a novel device for long-term storage
Helen C. O’Neill, Maya Nikoloska, HiuTung Ho, Alpesh Doshi, Walid Maalouf
Journal of Assisted Reproduction and Genetics, 2019
A novel myeloid cell in murine spleen defined through gene profiling
Ying‐Ying Hey, Terence J. O’Neill, Helen C. O’Neill
Journal of Cellular and Molecular Medicine, 2019
Targeting the Spleen as an Alternative Site for Hematopoiesis
Christie Short, Hong K. Lim, Jonathan Tan, Helen C. O'Neill
Bioessays, 2019
In assisted reproduction by IVF or ICSI, the rate at which embryos develop to the blastocyst stage is influenced by the fertilization method used: a split IVF/ICSI study
Barbara Speyer, Helen O’Neill, Wael Saab, Srividya Seshadri, Suzanne Cawood, Carleen Heath, Matthew Gaunt, Paul Serhal
Journal of Assisted Reproduction and Genetics, 2019
Identification of Stromal Cells in Spleen Which Support Myelopoiesis
Hong Kiat Lim, Helen C. O’Neill
Frontiers in Cell and Developmental Biology, 2019
Advancing a stem cell therapy for age-related macular degeneration
Helen C. O’Neill, Ioannis J. Limnios, Nigel L. Barnett
Current Stem Cell Research and Therapy, 2019
Identification of genes which regulate stromadependent in vitro hematopoiesis
Pravin Periasamy, Vinson Tran, Helen C. O’Neill
Plos One, 2018
MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis
Sawang Petvises, Pravin Periasamy, Helen C. O’Neill
BMC Immunology, 2018
In vitro murine hematopoiesis supported by signaling from a splenic stromal cell line
Hong Kiat Lim, Pravin Periasamy, Helen C. O’Neill
Stem Cells International, 2018
Genome Editing and Muscle Stem Cells as a Therapeutic Tool for Muscular Dystrophies
Veronica Pini, Jennifer E. Morgan, Francesco Muntoni, Helen C. O’Neill
Current Stem Cell Reports, 2017
Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development
Peter Papathanasiou, Sawang Petvises, Ying-Ying Hey, Andrew C. Perkins, Helen C. O’Neill
Plos One, 2017
Antigen presenting capacity of murine splenic myeloid cells
Ying-Ying Hey, Benjamin Quah, Helen C. O’Neill
BMC Immunology, 2017
Normal Levels of Sox9 Expression in the Developing Mouse Testis Depend on the TES/TESCO Enhancer, but This Does Not Act Alone
Nitzan Gonen, Alexander Quinn, Helen C. O’Neill, Peter Koopman, Robin Lovell-Badge
Plos Genetics, 2017
Antigen presenting properties of a myeloid dendritic-like cell in murine spleen
Ying-ying Hey, Helen C. O’Neill
Plos One, 2016
Delineation of a novel dendritic-like subset in human spleen
Sawang Petvises, Dipti Talaulikar, Helen C O’Neill
Cellular and Molecular Immunology, 2016
A Broad Overview and Review of CRISPR-Cas Technology and Stem Cells
Simon N. Waddington, Riccardo Privolizzi, Rajvinder Karda, Helen C. O’Neill
Current Stem Cell Reports, 2016
Redefining myeloid cell subsets in murine spleen
Ying-Ying Hey, Jonathan K. H. Tan, Helen C. O’Neill
Frontiers in Immunology, 2016
The B subunit of Escherichia coli heat-labile toxin alters the development and antigen-presenting capacity of dendritic cells
Jing Ji, Kristin L Griffiths, Peter J Milburn, Timothy R Hirst, Helen C O'Neill
Journal of Cellular and Molecular Medicine, 2015
Expression of TCR-Vβ peptides by murine bone marrow cells does not identify T-cell progenitors
Janice L. Abbey, Holger Karsunky, Thomas Serwold, Peter Papathanasiou, Irving L. Weissman, Helen C. O'Neill
Journal of Cellular and Molecular Medicine, 2015
Characterization of the effect of LPS on dendritic cell subset discrimination in spleen
Kristin L Griffiths, Jonathan KH Tan, Helen C O'Neill
Journal of Cellular and Molecular Medicine, 2014
Characterisation of dendritic cells arising from progenitors endogenous to murine spleen
Sawang Petvises, Helen C. O’Neill
Plos One, 2014
Extramedullary hematopoiesis leading to the production of a novel antigen-presenting cell type in murine spleen
Journal of Stem Cells, 2014
Spleen stroma maintains progenitors and supports long-term hematopoiesis
Helen O’Neill, Kristin Griffiths, Pravin Periasamy, Rebecca Hinton, Sawang Petvises, Ying-ying Hey, Jonathan Tan
Current Stem Cell Research and Therapy, 2014
Investigation into the prevalence of a novel dendritic-like cell subset in vivo
Kristin Lisa Griffiths, Jonathan Kah Huat Tan, Helen Christine O'Neill
Journal of Cellular and Molecular Medicine, 2013
Development of two distinct dendritic-like APCs in the context of splenic stroma
Pravin Periasamy, Sawang Petvises, Helen C. O’Neill
Frontiers in Immunology, 2013
Stroma-dependent development of two dendritic-like cell types with distinct antigen presenting capability
Pravin Periasamy, Helen C. O'Neill
Experimental Hematology, 2013
In vitro hematopoiesis reveals a novel dendritic-like cell present in murine spleen
Pravin Periasamy, Helen O’Neill
Current Stem Cell Research and Therapy, 2013
Distinct progenitor origin distinguishes a lineage of dendritic-like cells in spleen
Frontiers in Immunology, 2013
Novel splenic antigen-presenting cells derive from a Lin-c-kitlo progenitor
Pravin Periasamy, Jonathan K H Tan, Helen C OˈNeill
Journal of Leukocyte Biology, 2013
The spleen as a distinct site for dendritic cell haematopoiesis
New Developments in Cell Research, 2012
Murine spleen contains a diversity of myeloid and dendritic cells distinct in antigen presenting function
Ying Y. Hey, Helen C. O'Neill
Journal of Cellular and Molecular Medicine, 2012
Myelopoiesis in spleen-producing distinct dendritic-like cells
Jonathan K. H. Tan, Helen C. O’Neill
Journal of Cellular and Molecular Medicine, 2012
Hematopoiesis leading to a diversity of dendritic antigen-presenting cell types
Sawang Petvises, Helen C O'Neill
Immunology and Cell Biology, 2012
Technical advance: In vitro production of distinct dendritic-like antigen-presenting cells from self-renewing hematopoietic stem cells
Rebecca A Hinton, Helen C O'Neill
Journal of Leukocyte Biology, 2012
Distinct in vitro myelopoiesis is dependent on the self-renewal of hematopoietic progenitors
R. A. Hinton, P. Papathanasiou, H. C. O’Neill
Scandinavian Journal of Immunology, 2012
Spleen as a site for hematopoiesis of a distinct antigen presenting cell type
Helen C. O'Neill, Kristin L. Griffiths, Pravin Periasamy, Rebecca A. Hinton, Ying-Ying Hey, Sawang Petvises, Jonathan K. H. Tan
Stem Cells International, 2011
Myelopoiesis related to perinatal spleen
Rebecca Hinton, Sawang Petvises, Helen O'Neill
Immunology and Cell Biology, 2011
Identification of a novel antigen cross-presenting cell type in spleen
Jonathan K. H. Tan, Ben J. C. Quah, Kristin L. Griffiths, Pravin Periasamy, Ying-Ying Hey, Helen C. O’Neill
Journal of Cellular and Molecular Medicine, 2011
In Vitro haematopoiesis of a novel dendritic-like cell present in murine spleen
Jonathan K.H. Tan, Helen C. O'Neill
Current Stem Cell Research and Therapy, 2010
Authors' reply: The spleen as a site for hematopoiesis
Helen C. O'Neill, Jonathan K. H. Tan
Transplantation, 2010
Haematopoietic stem cells in spleen have distinct differentiative potential for antigen presenting cells
Jonathan K.H. Tan, Helen C. O’Neill
Journal of Cellular and Molecular Medicine, 2010
Delineation of precursors in murine spleen that develop in contact with splenic endothelium to give novel dendritic-like cells
Jonathan K. H. Tan, Pravin Periasamy, Helen C. O'Neill
Blood, 2010
Investigation of murine spleen as a niche for hematopoiesis
Jonathan K. H. Tan, Helen C. O'Neill
Transplantation, 2010
Spleen as a distinct site for dendritic cell haematopoiesis
Dendritic Cells Types Life Cycles and Biological Functions, 2010
Oncogenesis and cancer stem cells: Current opinions and future directions
Xiaoyu Cheng, Helen C. O’Neill
Journal of Cellular and Molecular Medicine, 2009
Splenic stromal niches support hematopoiesis of dendritic-like cells from precursors in bone marrow and spleen
Pravin Periasamy, Jonathan K.H. Tan, Kristin L. Griffiths, Helen C. O'Neill
Experimental Hematology, 2009
Dendritic cells as immune regulators: The mouse model
K.L. Griffiths, H.C. O’Neill
Journal of Cellular and Molecular Medicine, 2008
Gene signature of stromal cells which support dendritic cell development
Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence J. O’Neill, Helen C. O’Neill
Stem Cells and Development, 2008
Exosomes secreted by bacterially infected macrophages are proinflammatory
Helen C. O’Neill, Ben J. C. Quah
Science Signaling, 2008
Expression of T-cell receptor genes during early T-cell development
Janice L Abbey, Helen C O'Neill
Immunology and Cell Biology, 2008
Detection of spliced and unspliced forms of germline TCR-Vβ transcripts in extrathymic lymphoid sites
Janice L. Abbey, Helen C. O’Neill
Molecular Immunology, 2008
Mycoplasma contaminants present in exosome preparations induce polyclonal B cell responses
Ben J C Quah, Helen C O’Neill
Journal of Leukocyte Biology, 2007
Use of gene profiling to describe a niche for dendritic cell development
Geneviève Despars, Terence J O'Neill, Helen C O'Neill
Immunology and Cell Biology, 2007
Hematopoiesis of immature myeloid dendritic cells in stroma-dependent spleen long-term cultures occurs independently of NF-kB/RelB function
Jonathan K.H. Tan, Keping Ni, Fei Le, Helen C. O'Neill
Experimental Hematology, 2007
Concise review: Dendritic cell development in the context of the spleen microenvironment
Jonathan K. H. Tan, Helen C. O'Neill
Stem Cells, 2007
The role of stroma in hematopoiesis and dendritic cell development
Genevieve Despars, Jonathan Tan, Pravin Periasamy, Helen O'Neill, Helen C. O'Neill
Current Stem Cell Research and Therapy, 2007
Splenic endothelial cell lines support development of dendritic cells from bone marrow
Geneviève Despars, Helen C. O'Neill
Stem Cells, 2006
Cell surface expression of a peptide encoded by the unrearranged TCR-Vβ8.2 gene
Janice L. Abbey, Mark Hulett, Helen C. O’Neill
Molecular Immunology, 2006
Dendritic cell therapy for tolerance induction to stem cell transplants.
Helen O'Neill
Current Stem Cell Research Therapy, 2006
Heterogeneity amongst splenic stromal cell lines which support dendritic cell hematopoiesis
GENEVIÈVE DESPARS, HELEN C. O'NEILL
In Vitro Cellular and Developmental Biology Animal, 2006
The immunogenicity of dendritic cell-derived exosomes
Ben J.C. Quah, Helen C. O'Neill
Blood Cells Molecules and Diseases, 2005
Maturation requirements for dendritic cells in T cell stimulation leading to tolerance versus immunity
Jonathan K H Tan, Helen C O'Neill
Journal of Leukocyte Biology, 2005
Maturation of function in dendritic cells for tolerance and immunity
Ben J.C. Quah, Helen C. O'Neill
Journal of Cellular and Molecular Medicine, 2005
Molecular definition of an in vitro niche for dendritic cell development
Geneviève Despars, Keping Ni, Antoine Bouchard, Terence J. O'Neill, Helen C. O'Neill
Experimental Hematology, 2004
Preparation of label from small cell numbers for microarray screening
Heather L. Wilson, Helen C. O'Neill
OMICS A Journal of Integrative Biology, 2004
Germline transcription of multiple TCR-Vβ genes in cloned T-cell lines
Janice L Abbey, Helen C O'Neill
Immunology and Cell Biology, 2004
Limitations with in vitro production of dendritic cells using cytokines
Helen C O’Neill, Heather L Wilson
Journal of Leukocyte Biology, 2004
In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limeted T cell stimulation
B. Quah
International Immunology, 2004
A role for niches in the development of a multiplicity of dendritic cell subsets
Geneviève Despars, Helen C O'Neill
Experimental Hematology, 2004
Dendritic cell development in long-term spleen stromal cultures
Helen C. O'Neill, Heather L. Wilson, Ben Quah, Janice L. Abbey, Geneviève Despars, Keping Ni
Stem Cells, 2004
Identification of differentially expressed genes representing dendritic cell precursors and their progeny
Heather L. Wilson, Helen C. O'Neill
Blood, 2003
Murine dendritic cell development: Difficulties associated with subset analysis
Heather L Wilson, Helen C O'Neill
Immunology and Cell Biology, 2003
Dynamics of dendritic cell development from precursors maintained in stroma-dependent long-term cultures
Heather L Wilson, Helen C O'Neill
Immunology and Cell Biology, 2003
Development of dendritic cells from GM-CSF-/- mice in vitro: GM-CSF enhances production and survival of cells
Keping Ni, Helen C. O′Neill
Developmental Immunology, 2001
Manipulation of dendritic cells for immunotherapy of tumours
Today S Life Science, 2000
Improved FACS analysis confirms generation of immature dendritic cells in long-term stromal-dependent spleen cultures
Keping Ni, Helen C O’Neill
Immunology and Cell Biology, 2000
Identification of progenitor cells in long-term spleen stromal cultures that produce immature dendritic cells
Heather L. Wilson, Keping Ni, Helen C. O'Neill
Proceedings of the National Academy of Sciences of the United States of America, 2000
The application of dendritic cell-derived exosomes in tumour immunotherapy
Ben Quah, Helen C. O'Neill
Cancer Biotherapy and Radiopharmaceuticals, 2000
Proliferation of dendritic cell progenitors in long term culture is not dependent on granulocyte macrophage-colony stimulating factor
Heather L. Wilson, Keping Ni, Helen C. O'Neill
Experimental Hematology, 2000
Long-term stroma-dependent cultures are a consistent source of immunostimulatory dendritic cells
Helen C O’Neill, Keping Ni, Heather Wilson
Immunology and Cell Biology, 1999
Spleen stromal cells support haemopoiesis and in vitro growth of dendritic cells from bone marrow
Keping Ni, Helen O'Neill
British Journal of Haematology, 1999
Review: Dendritic cell immunotherapy for melanoma
May Hadzantonis, Helen ONeill
Cancer Biotherapy and Radiopharmaceuticals, 1999
Immunotherapeutic potential of dendritic cells generated in long term stroma-dependent cultures
HC O'Neill, N Jonas, H Wilson, K Ni
Cancer Biotherapy and Radiopharmaceuticals, 1999
Hemopoiesis in long-term stroma-dependent cultures from lymphoid tissue: Production of cells with myeloid/dendritic characteristics
K. Ni, H. C. O’Neill
In Vitro Cellular and Developmental Biology Animal, 1998
Specific transcription of the unrearranged TCR Vβ8.2 gene in lymphoid tissues occurs independently of V(D)J rearrangement
Christopher J Jolly, Helen C O'Neill
Immunology and Cell Biology, 1997
The role of dendritic cells in T cell activation
K Ni, HC O'Neill
Immunology and Cell Biology, 1997
Long-term stromal cultures produce dendritic-like cells
Keping Ni, H. C. O'Neill
British Journal of Haematology, 1997
Association of repeat sequences with integrated retroviruses in a murine leukaemia cell line
Ellen S.-P. Ho, Barbara van Leeuwen, Helen C. O'Neill
Leukemia Research, 1996
Expression of a TCR Vβ8.2 polypeptide from the unrearranged gene in a murine lymphoid precursor cell line
Christopher J. Jolly, Helen C. O'Neill
International Immunology, 1995
Leukaemogenic progression: The importance of differentiation and associated genetic events
Immunology and Cell Biology, 1995
Germline transcription and expression of Tcrb-V8 genes in peripheral mouse lymphoid tissues
HelenC. O'Neill, ChristopherJ. Jolly
Immunogenetics, 1995
Induction of leukemia in mice using a radiation leukemia virus-induced cell line: A model system for studying oncogenic progression
Ellen S.P. Ho, Terence J. O'Neill, Helen C. O'Neill
Leukemia Research, 1995
Growth regulation of a T-cell lymphoma via the T-cell receptor
Helen C. O'Neill
Leukemia Research, 1994
Erratum: T-Cell differentiative capacity of haematopoietic stem cells immortalized in vitro with radiation leukemia virus (Leukemia (1993) 7 (1281-1290))
Leukemia, 1994
Analysis of oncogenic progression in a radiation leukemia virus model
Leukemia, 1994
A murine retrovirus induces proliferation of unique lymphoid cell lines expressing T-cell-receptor structures utilizing common variable region α and β chain genes
Proceedings of the National Academy of Sciences of the United States of America, 1993
A gamma model for extra-binomial variation in dilution assays
Terence J. O'Neill, Helen C. O'Neill
Biometrics, 1993
Characterization of unique lymphoid cells derived from murine spleen which constitutively produce interleukin-6
Immunology, 1993
T-cell differentiative capacity of haematopoietic stem cells immortalized in vitro with radiation leukemia virus
Leukemia, 1993
Lymphoid precursor cell lines have the capacity to migrate to multiple lymphoid sites
Immunology, 1992
Unique lymphoid cell subset target to infection and proliferation induced in vitro by a murine leukemia virus
Leukemia, 1992
The diversity of retroviral diseases of the immune system
Immunology and Cell Biology, 1992
Lymphocyte Recirculation: the Need for Site‐Specific Receptors to Dictate T‐Lymphocyte Localization into Different Tissue Sites
Fredrik J. Frogner, Helen C. O'Neill
Scandinavian Journal of Immunology, 1992
Retroviral superantigens
Helen C. O'Neill, Chris Jolly
Immunology Today, 1992
Proliferation of the BCL1 B cell lymphoma induced by IL‐4 and IL‐5 is dependent on IL‐6 and GM‐CSF
Immunology and Cell Biology, 1992
Resistance to ectromelia virus infection in mice Analysis of H-2-linked gene effects
Helen C. O'Neill
Archives of Virology, 1991
Radiation leukemia virus-induced T-cell lymphomas with common T-cell receptor variable region structure and similar binding specificity for retrovirus
Leukemia, 1991
Prothymocyte seeding in the thymus
Helen C. O'Neill
Immunology Letters, 1991
Preferential expression of a common T cell receptor structure by T cells induced to proliferate in vitro with a murine retrovirus
Helen C. O'Neill
Cellular Immunology, 1991
Identification of Class I H‐2Db molecules primarily expressed by B lymphocytes in murine spleen
Immunology and Cell Biology, 1991
Binding of the BL/VL3 murine T cell lymphoma to radiation leukaemia virus is specific for viral env determinants
H. C. O'Neill
Journal of General Virology, 1991
Detection of Class I MHC antigens on glycosidase‐treated cells
Immunology and Cell Biology, 1991
Monoclonal antibodies which identify carbohydrate‐defined MHC Class I epitopes
Immunology and Cell Biology, 1991
Identification of molecules detected by three different anti-H-2K(k) monoclonal antibodies
HELEN C. O'NEILL
Hybridoma, 1991
The SCID-hu mouse. Human immune system in a mouse
Today S Life Science, 1990
Unique role for the T cell receptor in retrovirus binding by the C6VL thymoma
Helen C. O'Neill
International Immunology, 1990
Preferential usage of the Vβ8 gene family by CD4- CD8- T cell lines derived from spleen
Helen C. O'Neill
Cellular Immunology, 1990
Natural Anti-Self Reactivity Which Maps to MHC Class I Genes
Helen C. O'Neill
Immunobiology, 1990
Measurement of binding specificity between T cell lymphomas and murine leukemia viruses
Helen C. O'Neill, Irving L. Weissman
Journal of Immunological Methods, 1989
Antibody which defines a subset of bone marrow cells that can migrate to thymus
Immunology, 1989
Effects of anti-Class I antibodies on proliferation of murine T lymphocytes
Helen C. O'Neill
Cellular Immunology, 1988
Restricted blocking of cytotoxic T-cell function by anti-H-2K/D antibodies
Immunology, 1988
Interleukin-2 and antibody to the T cell receptor regulate proliferation of a radiation leukemia virus-transformed T cell lymphoma
Leukemia, 1988
Internalization of IL-2 by an IL-2 dependent murine T cell lymphoma expressing no detectable cell surface receptors for IL-2
Leukemia, 1988
Monosaccharide inhibition of cytotoxic T-cell function: Demonstration of clone-specific effects
Immunology, 1988
Isolation of a thymus-homing Lyt-2-, L3T4- T-cell line from mouse spleen
Helen C. O'Neill
Cellular Immunology, 1987
A subset of T cell receptors associated with L3T4 molecules mediates C6VL leukemia cell binding of its cognate retrovirus
Helen C O'Neill, Michael S McGrath, James P Allison, Irving L Weissman
Cell, 1987
A role for early cytotoxic T cells in resistance to ectromelia virus infection in mice.
H. C. O'Neill, M. Brenan
Journal of General Virology, 1987
Monoclonal antibodies specific for H-2K and H-2D antigens on cytotoxic T cells can inhibit their function
H C O'Neill
Proceedings of the National Academy of Sciences of the United States of America, 1986
Lymphocyte and lymphoma receptors utilized in differentiation, in homing, and in lymphomagenesis.
I. L. Weissman, M. S. McGrath, R. A. Reichert, W. M. Gallatin, S. Ezine, P. Fink, E. C. Butcher, J. Marian, H. C. O’Neill
Haematology and Blood Transfusion, 1985
VARIATION IN H‐2 ANTIGEN EXPRESSION ON LYMPHOMYELOID CELLS IN SEMI‐ALLOGENEIC IRRADIATION CHIMERAS
Helen, C. O'Neill, R. B. Ashman, P. F. Gallagher, C. E. Woodhams, R. V. Blanden
International Journal of Immunogenetics, 1984
A rapid, automated colorimetric assay for measuring antibody binding to cell surface antigens
Helen C. O'Neill, Christopher R. Parish
Journal of Immunological Methods, 1983
Mechanisms determining innate resistance to ectromelia virus infection in C57BL mice
H C O'Neill, R V Blanden
Infection and Immunity, 1983
H-2-linked control of resistance to Ectromelia virus infection in 1310 congenic mice
Helen C. O'Neill, R. V. Blandenr, T. J. O'Neill
Immunogenetics, 1983
Anti-P2 cytotoxic T cells in (P1 x P2)F1→P1 radiation chimeras
Behring Institute Mitteilungen, 1982
A sensitive and quantitative fluorescence assay for cell surface antigens
Terry J. Higgins, Helen C. O'neill, Christopher R. Parish
Journal of Immunological Methods, 1981
H-2U: A new region at the D end of the murine MHC
H. C. O'Neill, C. R. Parish
Immunogenetics, 1981
Monoclonal antibody detection of two classes of H-2Kk molecules
H ONEILL, C PARISH
Molecular Immunology, 1981
Monoclonal antibody detection of carbohydrate-defined and protein-defined H-2kK antigens
Helen C. O'neill, Christopher R. Parish, Terry J. Higgins
Molecular Immunology, 1981
Glycosyltransferases and T-cell recognition
Christopher R. Parish, Helen C. O'Neill, Terry J. Higgins
Immunology Today, 1981
Incomplete Tolerance to MHC Antigens in Irradiation Chimeras: Implications for MHC Restriction and Self Tolerance
R. V. Blanden, R. B. Ashman, Helen C. O'Neill, Cathy E. Woodhams, Ursula R. Kees, Marion E. Andrew
Immunological Reviews, 1981
A new procedure for analysing the relationship between different cell surface antigens
Christopher R. Parish, Helen C. O'Neill, Ian F.C. McKenzie
Journal of Immunological Methods, 1980
Quantitative variation in H-2-antigen expression - II. Evidence for a dominance pattern in H-2K and H-2D expression in F1 hybrid mice
Helen C. O'Neill
Immunogenetics, 1980
Quantitative variation in H-2-antigen expression - I. Estimation of H-2K and H-2D expression in different strains of mice
Helen C. O'Neill, I. F. C. McKenzie
Immunogenetics, 1980
Variation in H-2 antigen expression in F1 hybrid mice: Analysis using monoclonal antibodies
Helen C O'Neill, RV Blanden
Australian Journal of Experimental Biology and Medical Science, 1979

Helen Christine O'Neill

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