Soil characteristics drive contrasting patterns of association between symbiotic rhizobia of endemic and widespread Mimosa species in Brazil Luc Rouws, Alexandre Barauna, Chrizelle Beukes, Janaina R.C. Rouws, Sergio Miana de Faria, Eduardo Gross, Fabio Bueno dos Reis, Marcelo F. Simon, Marta Maluk, David W. Odee, Stephanie Fordeyn, Gregory Kenicer, J. Peter W. Young, Veronica M. Reis, Jerri Zilli, Euan K. James Applied Soil Ecology, 2024 Neotropical Beta-rhizobia have a particular affinity to the large legume (Fabaceae) genus Mimosa and some of its relatives in the tribe Mimosae of the Caesalpinioideae subfamily. However, little is still known about the ecology of this interaction, especially the relationship between the rhizobia of “widespread” pan-tropical Mimosa species like M. pudica and the rhizobia that nodulate endemic Mimosa species that are very restricted in their habitats. The objective of this study was to examine the microsymbionts of Mimosa spp. and other mimosoids in climates ranging from tropical to subtropical, humid to semi-arid, with varied soil characteristics and altitudes, with the aim of testing the hypothesis that widespread species have more cosmopolitan symbiont preferences than endemic ones. Nodules were sampled from >30 Mimosa spp. and related taxa in 13 Brazilian states covering all five national regions; many of the species were endemics or biome-restricted, but particular attention was also paid to sample nodules from the widespread species M. pudica at all locations. The Mimosa symbionts comprised 21 potential 16S rRNA and recA groups at the species level, with 17 belonging to the genus Paraburkholderia , including four lineages that may represent new species. The remaining genotypes consisted of 14 strains in two lineages of Cupriavidus that were mainly isolated from M. pudica growing at low altitudes, and a single lineage of Rhizobium also from M. pudica . In addition, a strain of Trinickia symbiotica was isolated from M. misera . It is concluded that diverse genotypes of Paraburkholderia dominate as symbionts of Mimosa in the acidic soils of its main center of radiation in Central Brazil but that Cupriavidus and Rhizobium comprise a significant minority of symbionts of widespread Mimosa spp., especially M. pudica , in lowland or disturbed areas with less acidic soils. Mimosa symbiont selection is thus driven either by edapho-climatic characteristics for widespread species and/or by co-evolution of the symbiotic partners for endemic species. • A survey of Mimosa -nodulating symbionts was conducted in thirteen Brazilian states. • Endemic Mimosa spp. are almost exclusively nodulated by Paraburkholderia strains. • Genomic comparisons indicate that these include several potential new species. • Widespread Mimosa spp. are also nodulated with Cupriavidus and Rhizobium strains. • The selection of symbionts by widespread Mimosa spp. depends on edaphic characters.
The radiation of nodulated Chamaecrista species from the rainforest into more diverse habitats has been accompanied by a reduction in growth form and a shift from fixation threads to symbiosomes Patricia Alves Casaes, José Miguel Ferreira dos Santos, Verônica Cordeiro Silva, Mariana Ferreira Kruschewsky Rhem, Matheus Martins Teixeira Cota, Sergio Miana de Faria, Juliana Gastaldello Rando, Euan K James, Eduardo Gross Journal of Experimental Botany, 2024 All non-Mimosoid nodulated genera in the legume subfamily Caesalpinioideae confine their rhizobial symbionts within cell wall-bound ‘fixation threads’ (FTs). The exception is the large genus Chamaecrista in which shrubs and subshrubs house their rhizobial bacteroids more intimately within symbiosomes, whereas large trees have FTs. This study aimed to unravel the evolutionary relationships between Chamaecrista growth habit, habitat, nodule bacteroid type, and rhizobial genotype. The growth habit, bacteroid anatomy, and rhizobial symbionts of 30 nodulated Chamaecrista species native to different biomes in the Brazilian state of Bahia, a major centre of diversity for the genus, was plotted onto an ITS-trnL-F-derived phylogeny of Chamaecrista. The bacteroids from most of the Chamaecrista species examined were enclosed in symbiosomes (SYM-type nodules), but those in arborescent species in the section Apoucouita, at the base of the genus, were enclosed in cell wall material containing homogalacturonan (HG) and cellulose (FT-type nodules). Most symbionts were Bradyrhizobium genotypes grouped according to the growth habits of their hosts, but the tree, C. eitenorum, was nodulated by Paraburkholderia. Chamaecrista has a range of growth habits that allow it to occupy several different biomes and to co-evolve with a wide range of (mainly) bradyrhizobial symbionts. FTs represent a less intimate symbiosis linked with nodulation losses, so the evolution of SYM-type nodules by most Chamaecrista species may have (i) aided the genus-wide retention of nodulation, and (ii) assisted in its rapid speciation and radiation out of the rainforest into more diverse and challenging habitats.
Nuclear energy and its role in decarbonization: Scenarios and perspectives Joel Augusto Moura Porto, Lander de Jesus Alves, Fábio Carvalho Nunes, Eduardo Gross, Hector Hugo Silva Medrado, Josilene da Silva Rocha, Marcelo Machado Viana, Ana Paula de Carvalho Teixeira, Majeti Narasimha Vara Prasad Decarbonization Strategies and Drivers to Achieve Carbon Neutrality for Sustainability, 2024
Relationship between clinical-epidemiological parameters and outcomes of patients with COVID-19 admitted to the intensive care unit: a report from a Brazilian hospital Maisah Meyhr D’Carmo Sodré, Uener Ribeiro dos Santos, Heitor Portella Povoas, Júlio Lenin Guzmán, Caroline Junqueira, Tayana Oliveira Trindade, Sandra Rocha Gadelha, Carla Cristina Romano, Aline Oliveira da Conceição, Eduardo Gross, Aline Silva, Rachel Passos Rezende, Renato Fontana, Camila Pacheco Silveira Martins da Mata, Lauro Juliano Marin, Luciana Debortoli de Carvalho Frontiers in Public Health, 2023 BackgroundPeople in low-income countries, especially those with low socio-economic conditions, are likelier to test positive for SARS-CoV-2. The unequal conditions of public health systems also increase the infection rate and make early identification and treatment of at-risk patients difficult. Here, we aimed to characterize the epidemiological profile of COVID-19 patients in intensive care and identify laboratory and clinical markers associated with death.Materials and methodsWe conducted an observational, descriptive, and cross-sectional study in a reference hospital for COVID-19 treatment in the Southern Region of Bahia State, in Brazil, to evaluate the epidemiological, clinical, and laboratory characteristics of COVID-19 patients admitted to the intensive care unit (ICU). Additionally, we used the area under the curve (AUC) to classify survivors and non-survivors and a multivariate logistic regression analysis to assess factors associated with death. Data was collected from the hospital databases between April 2020 and July 2021.ResultsThe use of bladder catheters (OR 79.30; p < 0.0001) and central venous catheters (OR, 45.12; p < 0.0001) were the main factors associated with death in ICU COVID-19 patients. Additionally, the number of non-survivors increased with age (p < 0.0001) and prolonged ICU stay (p < 0.0001). Besides, SAPS3 presents a higher sensibility (77.9%) and specificity (63.1%) to discriminate between survivors and non-survivor with an AUC of 0.79 (p < 0.0001).ConclusionWe suggest that multi-laboratory parameters can predict patient prognosis and guide healthcare teams toward more assertive clinical management, better resource allocation, and improved survival of COVID-19 patients admitted to the ICU.
Uranium transfer in grasses grown on mining waste and natural soil Lander de Jesus Alves, Eduardo Gross, Pedro Antônio Oliveira Mangabeira, Luana Novaes Santos, Irailde da Silva Santos, Fábio Carvalho Nunes, Hector Hugo Silva Medrado Journal of Environmental Radioactivity, 2022
The innovation of the symbiosome has enhanced the evolutionary stability of nitrogen fixation in legumes Sergio M. de Faria, Jens J. Ringelberg, Eduardo Gross, Erik J. M. Koenen, Domingos Cardoso, George K. D. Ametsitsi, John Akomatey, Marta Maluk, Nisha Tak, Hukam S. Gehlot, Kathryn M. Wright, Neung Teaumroong, Pongpan Songwattana, Haroldo C. de Lima, Yves Prin, Charles E. Zartman, Janet I. Sprent, Julie Ardley, Colin E. Hughes, Euan K. James New Phytologist, 2022 Summary Nitrogen‐fixing symbiosis is globally important in ecosystem functioning and agriculture, yet the evolutionary history of nodulation remains the focus of considerable debate. Recent evidence suggesting a single origin of nodulation followed by massive parallel evolutionary losses raises questions about why a few lineages in the N2‐fixing clade retained nodulation and diversified as stable nodulators, while most did not. Within legumes, nodulation is restricted to the two most diverse subfamilies, Papilionoideae and Caesalpinioideae, which show stable retention of nodulation across their core clades. We characterize two nodule anatomy types across 128 species in 56 of the 152 genera of the legume subfamily Caesalpinioideae: fixation thread nodules (FTs), where nitrogen‐fixing bacteroids are retained within the apoplast in modified infection threads, and symbiosomes, where rhizobia are symplastically internalized in the host cell cytoplasm within membrane‐bound symbiosomes (SYMs). Using a robust phylogenomic tree based on 997 genes from 147 Caesalpinioideae genera, we show that losses of nodulation are more prevalent in lineages with FTs than those with SYMs. We propose that evolution of the symbiosome allows for a more intimate and enduring symbiosis through tighter compartmentalization of their rhizobial microsymbionts, resulting in greater evolutionary stability of nodulation across this species‐rich pantropical legume clade.
Ex-vivo mucolytic and anti-inflammatory activity of BromAc in tracheal aspirates from COVID-19 Jordana Grazziela A. Coelho dos Reis, Geovane Marques Ferreira, Alice Aparecida Lourenço, Ágata Lopes Ribeiro, Camila Pacheco da Silveira Martins da Mata, Patrícia de Melo Oliveira, Daisymara Priscila de Almeida Marques, Linziane Lopes Ferreira, Felipe Alves Clarindo, Murillo Ferreira da Silva, Heitor Portella Póvoas Filho, Nilson Roberto Ribeiro Oliveira, Maisah Meyhr D’Carmo Sodré, Sandra Rocha Gadelha, George Rego Albuquerque, Bianca Mendes Maciel, Ana Paula Melo Mariano, Mylene de Melo Silva, Renato Fontana, Lauro Juliano Marin, Renata Santiago Alberto Carlos, Amanda Teixeira Sampaio Lopes, Fabrício Barbosa Ferreira, Uener Ribeiro dos Santos, Íris Terezinha Santos de Santana, Hllytchaikra Ferraz Fehlberg, Rachel Passos Rezende, João Carlos T. Dias, Eduardo Gross, Gisele Assis Castro Goulart, Marie Gabriele Santiago, Ana Paula Motta Lavigne de Lemos, Aline O. da Conceição, Carla Cristina Romano, Luciana Debortoli de Carvalho, Olindo Assis Martins Filho, Claudio Almeida Quadros, David L. Morris, Sarah J. Valle Biomedicine and Pharmacotherapy, 2022 COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2, which continues to be a public health threat. COVID-19 is principally a respiratory disease and is often associated with sputum retention and cytokine storm, for which there are limited therapeutic options. In this regard, we evaluated the use of BromAc®, a combination of Bromelain and Acetylcysteine (NAC). Both drugs present mucolytic effect and have been studied to treat COVID-19. Therefore, we sought to examine the mucolytic and anti-inflammatory effect of BromAc® in tracheal aspirate samples from critically ill COVID-19 patients requiring mechanical ventilation. METHOD: Tracheal aspirate samples from COVID-19 patients were collected following next of kin consent and mucolysis, rheometry and cytokine analysis using Luminex kit was performed. RESULTS: BromAc® displayed a robust mucolytic effect in a dose dependent manner on COVID-19 sputum ex vivo. BromAc® showed anti-inflammatory activity, reducing the action of cytokine storm, chemokines including MIP-1alpha, CXCL8, MIP-1b, MCP-1 and IP-10, and regulatory cytokines IL-5, IL-10, IL-13 IL-1Ra and total reduction for IL-9 compared to NAC alone and control. BromAc® acted on IL-6, demonstrating a reduction in G-CSF and VEGF-D at concentrations of 125 and 250 µg. CONCLUSION: These results indicate robust mucolytic and anti-inflammatory effect of BromAc® ex vivo in tracheal aspirates from critically ill COVID-19 patients, indicating its potential to be further assessed as pharmacological treatment for COVID-19.
Growth, leaf gas exchange and mycorrhizal colonization of three medicinal species submitted to different irradiance levels Viviane Maria Barazetti, Eduardo Gross, George Andrade Sodré, Ândrea Carla Dalmolin, Larissa Corrêa do Bomfim Costa, Miguel Antônio Quinteiro Ribeiro Ciencia Rural, 2022 This study o evaluated growth, leaf gas exchange and arbuscular mycorrhizal fungi root colonization in three medicinal plant species under different irradiance intensities. Fridericia chica (Bonpl.) L.G.Lohmann, Mikania laevigata Sch.Bip. ex Baker and Varronia curassavica Jacq. were propagated by cutting and cultivated for 120 days in artificially shaded environments using black shade-type screens, obtaining four irradiance levels: 100%, 70%, 50% and 30%. The experimental design was completely randomized in a 3 x 4 factorial scheme (three plant species and four irradiation levels) with seven replicates. The three medicinal species showed higher liquid assimilation, mass growth and arbuscular mycorrhizal fungi root colonization rates when exposed to environments with 70% light availability. In relation to physiological responses, V. curassavica presented higher photosynthetic rate, stomatal conductance and transpiration when submitted to 70% irradiance, being able to be cultivated in more open environments with higher irradiation levels. Conversely F. chica and M. laevigata presented shade tolerance characteristics. At the initial growth phase, the results obtained can be used as indicators to recommend the ideal cultivation environment for these species in agroforestry systems.
Substrate and quality mangosteen seedlings Gedeon Almeida Gomes Júnior, Rafaela Alves Pereira, Danillo José dos Santos, George Andrade Sodré, Eduardo Gross Revista Brasileira De Fruticultura, 2019
Burkholderia diazotrophica sp. nov., isolated from root nodules of Mimosa spp Shih-Yi Sheu, Jui-Hsing Chou, Cyril Bontemps, Geoffrey N. Elliott, Eduardo Gross, Fabio Bueno dos Reis Junior, Rémy Melkonian, Lionel Moulin, Euan K. James, Janet I. Sprent, J. Peter W. Young, Wen-Ming Chen International Journal of Systematic and Evolutionary Microbiology, 2013
Legume-nodulating betaproteobacteria: Diversity, host range, and future prospects Prasad Gyaneshwar, Ann M. Hirsch, Lionel Moulin, Wen-Ming Chen, Geoffrey N. Elliott, Cyril Bontemps, Paulina Estrada-de los Santos, Eduardo Gross, Fabio Bueno dos Reis, Janet I. Sprent, J. Peter W. Young, Euan K. James Molecular Plant Microbe Interactions, 2011
Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil Fábio Bueno dos Reis Jr, Marcelo F. Simon, Eduardo Gross, Robert M. Boddey, Geoffrey N. Elliott, Nicolau E. Neto, M. de Fatima Loureiro, Luciano P. de Queiroz, Maria Rita Scotti, Wen‐Ming Chen, Agneta Norén, Maria C. Rubio, Sergio M. de Faria, Cyril Bontemps, Silvia R. Goi, J. Peter W. Young, Janet I. Sprent, Euan K. James New Phytologist, 2010
Burkholderia species are ancient symbionts of legumes CYRIL BONTEMPS, GEOFFREY N. ELLIOTT, MARCELO F. SIMON, FÁBIO B. DOS REIS JÚNIOR, EDUARDO GROSS, REBECCA C. LAWTON, NICOLAU ELIAS NETO, MARIA de FÁTIMA LOUREIRO, SERGIO M. DE FARIA, JANET I. SPRENT, EUAN K. JAMES, J. PETER W. YOUNG Molecular Ecology, 2010