Dr. Supriya Kumari is a postdoctoral researcher with extensive experience in molecular biology, microbiology, and chemical biology. She earned her Ph.D. in Life Science from the National Institute of Technology, Rourkela, where she studied the bioremediation potential of marine bacteria. Her postdoctoral work at the National Institute of Science Education and Research (NISER), Bhubaneswar, focused on cancer biology, bacterial gene regulation, and CRISPR-based molecular systems. Dr. Kumari has contributed to over 20 peer-reviewed publications in reputed journals and has received awards including the DST Young Scientist Travel Award and an outstanding poster award at an international conference in Spain. Her technical expertise spans microbial and mammalian cell culture, gene editing, fluorescence/confocal microscopy, and molecular assays.
EDUCATION
ph.d (life science)
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Agricultural and Biological Sciences, Animal Science and Zoology, Molecular Biology
Taxonomy and Characterization of Biofilm Forming Polycyclic Aromatic Hydrocarbon Degrading Bacteria from Marine Environments Neelam Mangwani, Supriya Kumari, Surajit Das Polycyclic Aromatic Compounds, 2021 Marine environment is a dynamic habitat for survival of many microbes with unique metabolic potential. Biofilm formation benefits the marine bacteria to thrive and persist even in hostile environments. Besides, biofilm formation ability of bacteria enhances the efficiency of bioremediation of organic pollutants. In the present study, biofilm forming and polycyclic aromatic hydrocarbon (phenanthrene and pyrene) degrading bacteria were isolated and taxonomic identification was carried out by 16S rRNA gene sequencing. The isolates were able to grow on multiple aromatic hydrocarbons such as toluene, biphenyl, anthracene and naphthalene. The potential isolates were characterized by 16S rRNA gene sequencing. The isolates were identified and belong to the genera Pseudomonas, Stenotrophomonas, Paenibacillus, Alcaligenes, Sporosarcina and Lysinibacillus. Proteobacteria were found to be leading bacterial group at the pollutant sites. 75% of the isolates screened were able to establish biofilm and grow on either of aromatic hydrocarbon. This study shows the dominance of biofilm forming bacteria with ability to grow on multiple organic compounds in the marine environment.
Diversity, structure and regulation of microbial metallothionein: Metal resistance and possible applications in sequestration of toxic metals Shreosi Chatterjee, Supriya Kumari, Sonalin Rath, Monika Priyadarshanee, Surajit Das Metallomics, 2020 Metallothioneins (MTs) are a group of cysteine-rich, universal, low molecular weight proteins distributed widely in almost all major taxonomic groups ranging from tiny microbes to highly organized vertebrates. The primary function of this protein is storage, transportation and binding of metals, which enable microorganisms to detoxify heavy metals. In the microbial world, these peptides were first identified in a cyanobacterium Synechococcus as the SmtA protein which exhibits high affinity towards rising level of zinc and cadmium to preserve metal homeostasis in a cell. In yeast, MTs aid in reserving copper and confer protection against copper toxicity by chelating excess copper ions in a cell. Two MTs, CUP1 and Crs5, originating from Saccharomyces cerevisiae predominantly bind to copper though are capable of binding with zinc and cadmium ions. MT superfamily 7 is found in ciliated protozoa which show high affinity towards copper and cadmium. Several tools and techniques, such as western blot, capillary electrophoresis, inductively coupled plasma, atomic emission spectroscopy and high performance liquid chromatography, have been extensively utilized for the detection and quantification of microbial MTs which are utilized for the efficient remediation and sequestration of heavy metals from a contaminated environment.
Half sandwich based rhodamine - hydrazone single molecule probe: Light responsive, metal sensing and imaging properties S. Dewangan, T. Barik, S. Mishra, S. Mawatwal, S. Kumari, S. Giri, S. Das, R. Dhiman, C. Wölper, S. Chatterjee Applied Organometallic Chemistry, 2018 Functionalization of cymantrenyl hydrazone system with rhodamine based fluorescent tag led to the formation of a significant molecule containing both, a half sandwich and a rhodamine fragments, [(CO)3Mn{(η5‐C5H4) C (CH3)═NNC (O)‐R}] (R = Rhodamine‐6G (1), Rhodamine B (2)), with selective metal ion sensing, light responsive and molecular switching behavior. The molecule (1) was structurally characterized by single crystal X‐ray diffraction study. We have also explored Hg2+ recognition and photo ‐ sensing properties of compound 1 within a bacterial strain and THP‐1 cancer cell lines to understand their potential in applications related to bioimaging, uptake and bioremediation activities. The study showed some significant intracellular metal recognition and imaging characteristics at different metal concentration.
Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen Aeromonas hydrophila by tannic acid, a potent quorum quencher Bhakti Patel, Supriya Kumari, Rajanya Banerjee, Mrinal Samanta, Surajit Das Biofouling, 2017 The quorum sensing (QS) phenomenon regulates a myriad of pathogenic traits in the biofilm forming fish pathogen, Aeromonas hydrophila. Blocking the QS mechanism of A. hydrophila is a novel strategy to prevent disease in fish. This study evaluated the effect of tannic acid, a QS inhibitor, on A. hydrophila-associated QS regulated phenomena. A streaking assay with Chromobacterium violaceum (CVO26) reported the presence of N-acyl homoserine lactone (AHL) in A. hydrophila, which was confirmed by HPLC and GC-MS analysis. Tannic acid-treated A. hydrophila showed a considerable reduction in violacein production, blood haemolysis activity and the pattern of swarming motility. Biofilm formation was significantly reduced (p < 0.001) (up to 95%), after tannic acid treatment for 48 h. Analysis by qRT-PCR revealed significant downregulation (p < 0.001) of AhyI and AhyR transcripts in A. hydrophila after tannic acid treatment. Co-stimulation of Catla catla with A. hydrophila and tannic acid attenuated pathogen-induced skin haemorrhages and increased the relative survival rate up to 86.6%. The study provides a mechanistic basis of tannic acid as a QS blocker and indicates its therapeutic potential against A. hydrophila-induced pathogenesis.
Bacterial biofilms and quorum sensing: fidelity in bioremediation technology Neelam Mangwani, Supriya Kumari, Surajit Das Biotechnology and Genetic Engineering Reviews, 2017 Increased contamination of the environment with toxic pollutants has paved the way for efficient strategies which can be implemented for environmental restoration. The major problem with conventional methods used for cleaning of pollutants is inefficiency and high economic costs. Bioremediation is a growing technology having advanced potential of cleaning pollutants. Biofilm formed by various micro-organisms potentially provide a suitable microenvironment for efficient bioremediation processes. High cell density and stress resistance properties of the biofilm environment provide opportunities for efficient metabolism of number of hydrophobic and toxic compounds. Bacterial biofilm formation is often regulated by quorum sensing (QS) which is a population density-based cell–cell communication process via signaling molecules. Numerous signaling molecules such as acyl homoserine lactones, peptides, autoinducer-2, diffusion signaling factors, and α-hydroxyketones have been studied in bacteria. Genetic alteration of QS machinery can be useful to modulate vital characters valuable for environmental applications such as biofilm formation, biosurfactant production, exopolysaccharide synthesis, horizontal gene transfer, catabolic gene expression, motility, and chemotaxis. These qualities are imperative for bacteria during degradation or detoxification of any pollutant. QS signals can be used for the fabrication of engineered biofilms with enhanced degradation kinetics. This review discusses the connection between QS and biofilm formation by bacteria in relation to bioremediation technology.
Global Scenario of Antimicrobial Resistance: A Review of the Homoeopathic Perspective in Mitigating this Menace. RH Narula, A Nagar, S Kumari, R Mittal, S Ramteke, S Kaushik Tropical Journal of Natural Product Research 9 (10) , 2025 2025
PIDA-mediated synthesis of kynurenine derivatives by oxidative fragmentation of the tryptophan scaffold CK Jena, A Patnaik, SS Nayak, S Kumari, A Panda, M Dixit, NK Sharma Organic & Biomolecular Chemistry 23 (13), 3208-3223 , 2025 2025
Synthesis and DNA cleavage studies of rationally designed metallopeptides from N-salicyl-AA-picolamide (SAP) and Cu (ii) ions SS Panda, S Kumari, M Dixit, NK Sharma New Journal of Chemistry 48 (45), 19188-19194 , 2024 2024 Citations: 1
N- Salicyl-AA n - picolamide Foldameric Peptides Exhibit Quorum Sensing Inhibition of Pseudomonas aeruginosa (PA14) SS Panda, S Kumari, M Dixit, NK Sharma ACS omega 8 (33), 30349-30358 , 2023 2023 Citations: 8
Cu‐Catalyzed Synthesis of Alkylaminotroponyl Sulfones as Pseudomonas Aeruginosa Quorum Sensing Inhibitors Targeting lasI/R QS Circuitry S Meher, S Kumari, M Dixit, NK Sharma Chemistry–An Asian Journal 17 (23), e202200866 , 2022 2022 Citations: 8
Nonbenzenoid BODIPY Analogues: Synthesis, Structural Organization, Photophysical Studies, and Cell Internalization of Biocompatible N - Alkyl -Aminotroponyl Difluoroboron … B Bhusana Palai, S Kumari, M Dixit, NK Sharma ACS omega 7 (31), 27347-27358 , 2022 2022 Citations: 3
Taxonomy and characterization of biofilm forming polycyclic aromatic hydrocarbon degrading bacteria from marine environments N Mangwani, S Kumari, S Das Polycyclic Aromatic Compounds 41 (6), 1249-1262 , 2021 2021 Citations: 28
Naphthalene catabolism by biofilm forming marine bacterium Pseudomonas aeruginosa N6P6 and the role of quorum sensing in regulation of dioxygenase gene S Kumari, N Mangwani, S Das Journal of applied microbiology 130 (4), 1217-1231 , 2021 2021 Citations: 28
Diversity, structure and regulation of microbial metallothionein: metal resistance and possible applications in sequestration of toxic metals S Chatterjee, S Kumari, S Rath, M Priyadarshanee, S Das Metallomics 12 (11), 1637-1655 , 2020 2020 Citations: 116
Expression of metallothionein encoding gene bmtA in biofilm-forming marine bacterium Pseudomonas aeruginosa N6P6 and understanding its involvement in Pb … S Kumari, S Das Environmental Science and Pollution Research 26 (28), 28763-28774 , 2019 2019 Citations: 76
Half sandwich based rhodamine‐hydrazone single molecule probe: Light responsive, metal sensing and imaging properties S Dewangan, T Barik, S Mishra, S Mawatwal, S Kumari, S Giri, S Das, ... Applied Organometallic Chemistry 32 (12), e4612 , 2018 2018 Citations: 18
Bioremediation potential of biofilm forming lead resistant and naphthalene utilizing marine bacteria S Kumari 2018
Ca-alginate as a support matrix for Pb (II) biosorption with immobilized biofilm associated extracellular polymeric substances of Pseudomonas aeruginosa N6P6 S Kumari, S Mahapatra, S Das Chemical Engineering Journal 328, 556-566 , 2017 2017 Citations: 97
Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen Aeromonas hydrophila by tannic acid, a potent quorum quencher B Patel, S Kumari, R Banerjee, M Samanta, S Das Biofouling 33 (7), 580-590 , 2017 2017 Citations: 51
Marine bacterial biofilms in bioremediation of polycyclic aromatic hydrocarbons (PAHs) under terrestrial condition in a soil microcosm N Mangwani, S Kumari Pedosphere 27 (3), 548-558 , 2017 2017 Citations: 65
Interaction of Pb(II) and biofilm associated extracellular polymeric substances of a marine bacterium Pseudomonas pseudoalcaligenes NP103 S Kumari, N Mangwani, S Das Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 173, 655-665 , 2017 2017 Citations: 45
Effect of synthetic N-acylhomoserine lactones on cell–cell interactions in marine Pseudomonas and biofilm mediated degradation of polycyclic aromatic hydrocarbons N Mangwani, S Kumari, S Das Chemical Engineering Journal 302, 172-186 , 2016 2016 Citations: 65
Marine bacterial exopolysaccharides: functional diversity and prospects in environmental restoration J Chakraborty, N Mangwani, HR Dash, S Kumari, H Kumar, S Das Marine glycobiology, 255-274 , 2016 2016 Citations: 8
Synergistic effect of quorum sensing genes in biofilm development and PAHs degradation by a marine bacterium S Kumari, N Mangwani, S Das Bioengineered 7 (3), 205-211 , 2016 2016 Citations: 43
Bacterial biofilms and quorum sensing: fidelity in bioremediation technology N Mangwani, S Kumari, S Das Biotechnology and Genetic Engineering Reviews 32 (1-2), 43-73 , 2016 2016 Citations: 169
MOST CITED SCHOLAR PUBLICATIONS
Marine bacteria: potential candidates for enhanced bioremediation HR Dash, N Mangwani, J Chakraborty, S Kumari, S Das Applied microbiology and biotechnology 97 (2), 561-571 , 2013 2013 Citations: 355
Bacterial biofilms and quorum sensing: fidelity in bioremediation technology N Mangwani, S Kumari, S Das Biotechnology and Genetic Engineering Reviews 32 (1-2), 43-73 , 2016 2016 Citations: 169
Understanding molecular identification and polyphasic taxonomic approaches for genetic relatedness and phylogenetic relationships of microorganisms S Das, HR Dash, N Mangwani, J Chakraborty, S Kumari Journal of microbiological methods 103, 80-100 , 2014 2014 Citations: 166
Characterization of Stenotrophomonas acidaminiphila NCW‐702 biofilm for implication in the degradation of polycyclic aromatic hydrocarbons N Mangwani, SK Shukla, S Kumari, TS Rao, S Das Journal of applied microbiology 117 (4), 1012-1024 , 2014 2014 Citations: 118
Diversity, structure and regulation of microbial metallothionein: metal resistance and possible applications in sequestration of toxic metals S Chatterjee, S Kumari, S Rath, M Priyadarshanee, S Das Metallomics 12 (11), 1637-1655 , 2020 2020 Citations: 116
Involvement of quorum sensing genes in biofilm development and degradation of polycyclic aromatic hydrocarbons by a marine bacterium Pseudomonas aeruginosa N6P6 N Mangwani, S Kumari, S Das Applied Microbiology and Biotechnology 99 (23), 10283-10297 , 2015 2015 Citations: 102
Ca-alginate as a support matrix for Pb (II) biosorption with immobilized biofilm associated extracellular polymeric substances of Pseudomonas aeruginosa N6P6 S Kumari, S Mahapatra, S Das Chemical Engineering Journal 328, 556-566 , 2017 2017 Citations: 97
Expression of metallothionein encoding gene bmtA in biofilm-forming marine bacterium Pseudomonas aeruginosa N6P6 and understanding its involvement in Pb … S Kumari, S Das Environmental Science and Pollution Research 26 (28), 28763-28774 , 2019 2019 Citations: 76
Effect of biofilm parameters and extracellular polymeric substance composition on polycyclic aromatic hydrocarbon degradation N Mangwani, SK Shukla, S Kumari, S Das, TS Rao RSC advances 6 (62), 57540-57551 , 2016 2016 Citations: 75
Marine bacterial biofilms in bioremediation of polycyclic aromatic hydrocarbons (PAHs) under terrestrial condition in a soil microcosm N Mangwani, S Kumari Pedosphere 27 (3), 548-558 , 2017 2017 Citations: 65
Effect of synthetic N-acylhomoserine lactones on cell–cell interactions in marine Pseudomonas and biofilm mediated degradation of polycyclic aromatic hydrocarbons N Mangwani, S Kumari, S Das Chemical Engineering Journal 302, 172-186 , 2016 2016 Citations: 65
Disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen Aeromonas hydrophila by tannic acid, a potent quorum quencher B Patel, S Kumari, R Banerjee, M Samanta, S Das Biofouling 33 (7), 580-590 , 2017 2017 Citations: 51
Interaction of Pb(II) and biofilm associated extracellular polymeric substances of a marine bacterium Pseudomonas pseudoalcaligenes NP103 S Kumari, N Mangwani, S Das Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 173, 655-665 , 2017 2017 Citations: 45
Synergistic effect of quorum sensing genes in biofilm development and PAHs degradation by a marine bacterium S Kumari, N Mangwani, S Das Bioengineered 7 (3), 205-211 , 2016 2016 Citations: 43
Phenotypic Switching in Biofilm-Forming Marine Bacterium Paenibacillus lautus NE3B01 N Mangwani, S Kumari, SK Shukla, TS Rao, S Das Current microbiology 68 (5), 648-656 , 2014 2014 Citations: 32
Taxonomy and characterization of biofilm forming polycyclic aromatic hydrocarbon degrading bacteria from marine environments N Mangwani, S Kumari, S Das Polycyclic Aromatic Compounds 41 (6), 1249-1262 , 2021 2021 Citations: 28
Naphthalene catabolism by biofilm forming marine bacterium Pseudomonas aeruginosa N6P6 and the role of quorum sensing in regulation of dioxygenase gene S Kumari, N Mangwani, S Das Journal of applied microbiology 130 (4), 1217-1231 , 2021 2021 Citations: 28
Half sandwich based rhodamine‐hydrazone single molecule probe: Light responsive, metal sensing and imaging properties S Dewangan, T Barik, S Mishra, S Mawatwal, S Kumari, S Giri, S Das, ... Applied Organometallic Chemistry 32 (12), e4612 , 2018 2018 Citations: 18
Low-voltage producing microbial fuel cell constructs using biofilm-forming marine bacteria S Kumari, N Mangwani, S Das Current Science, 925-932 , 2015 2015 Citations: 16
N- Salicyl-AA n - picolamide Foldameric Peptides Exhibit Quorum Sensing Inhibition of Pseudomonas aeruginosa (PA14) SS Panda, S Kumari, M Dixit, NK Sharma ACS omega 8 (33), 30349-30358 , 2023 2023 Citations: 8
