Selective cytotoxicity of solamargine via oxidative stress and caspase-independent mechanisms in human glioblastoma cells Arthur Barcelos Ribeiro, Marcela de Melo Junqueira, Ricardo Andrade Furtado, Jairo Kenupp Bastos, Denise Crispim Tavares Investigational New Drugs, 2026 Summary IDH-wild-type glioblastoma (IDH-wildtype GB) is an aggressive and genetically heterogeneous tumor characterized by intrinsic resistance to radiotherapy and chemotherapy, while surgical resection remains inherently limited by its diffuse infiltrative growth, leading to poor clinical outcomes. Natural products such as solamargine (SM), a steroidal glycoalkaloid with cytotoxic and antitumor properties, have emerged as potential adjuvant strategies. Here, we investigated the effects of SM on proliferation, clonogenic survival, morphology and migration of IDH-wildtype GB cell lines (U-87MG, U-251MG and T98-G) and non-tumoral astrocytes under normoxic and hypoxic conditions, as well as its interaction with temozolomide (TMZ). Under normoxia, SM reduced cell viability in a dose- and time-dependent manner, with IC₅₀ values between 5.04 and 9.53 μM and showed enhanced cytotoxicity under hypoxia. TMZ alone displayed modest activity, and its combination with SM produced predominantly antagonistic effects. Clonogenic assays confirmed the antiproliferative potential of SM, with significant inhibition of colony formation at 2.5 μM. SM induced marked morphological alterations but did not significantly impair migration in wound-healing assays. In U-87MG cells, SM triggered G₂/M cell-cycle arrest, increased intracellular reactive oxygen species generation, and elevated γH2AX protein expression, indicating oxidative stress-associated DNA damage. However, cleaved caspase-3 and p53 were not detected, suggesting a predominantly non-apoptotic mode of cell death. Together, these findings support SM as a promising candidate for IDH-wildtype GB therapy and underscore the need for further studies to clarify its mechanisms of action and optimize its therapeutic use.
Evaluation of anti-inflammatory activity of kaurenol: Experimental evaluation and mechanistic insights Ricardo Andrade Furtado, Samir A. Ross, Silvio de Almeida Junior, Rafael Paranhos de Mendonça, Cristiane Teixeira Vilhena Bernardes, et al. Fundamental and Clinical Pharmacology, 2024 BackgroundKaurenol, a diterpene alcohol found in Copaifera langsdorffii Desf. (known as “copaiba”), is historically used in traditional medicine for inflammatory conditions.ObjectivesThis study aims to comprehensively assess the potential anti‐inflammatory and antinociceptive properties of kaurenol.MethodsTo this end, the following experiments were conducted to evaluated toxicity: locomotor performance and acute toxicity; nociception: acetic acid‐induced writhing and formalin‐induced antinociception; and anti‐inflammatory activity: carrageenan and dextran‐induced paw edema at 10, 20, and 40 mg/kg, and measurement of nitric oxide (NO), tumor necrosis factor alpha (TNF‐α), interleukin‐6 (IL‐6), and interleukin‐10 (IL‐10) in macrophages at 1, 3, and 9 μg/ml.ResultsKaurenol did not show significant locomotor changes, acute toxicity, and central analgesic activity in the first phase of formalin test at dosages tested. Kaurenol showed 53%, 64%, 64%, and 58% of inhibition in the acetic acid‐induced writhing, second phase of formalin test, carrageenan and dextran‐induced paw edema, respectively.ConclusionThe anti‐inflammatory activity was associated with the regulation of NO release and probably with the regulation of mediators, such as serotonin and prostaglandin in vascular permeability, as well as by being associated with the regulation of IL‐6 and IL‐10. Kaurenol display anti‐inflammatory activity but has no analgesic activity.
Solanum glycoalkaloid solamargine inhibits growth of pediatric high-grade glioma cells under normoxia and hypoxia conditions AB Ribeiro, MM Junqueira, RA Furtado, JK Bastos, D Crispim Tavares Journal of Toxicology and Environmental Health, Part A 89 (5), 237-249 , 2026 2026
Selective cytotoxicity of solamargine via oxidative stress and caspase-independent mechanisms in human glioblastoma cells AB Ribeiro, M de Melo Junqueira, RA Furtado, JK Bastos, DC Tavares Investigational New Drugs, 1-9 , 2026 2026
Evaluation of the biological potential of crude extracts of Brazilian medicinal plants in a social context and for safe use in human health JL Damasceno, SD Ozelin, LF Leandro, MALV Ambrosio, LA Garcia, ... Journal of Ethnopharmacology, 120967 , 2025 2025 Citations: 2
In vitro acaricidal effects of essential oils against Rhipicephalus microplus via bioinformatics targeting of triosephosphate isomerase PC Colombo, AG Batista, JG Martins, GN de Oliveira, MT da Silva Oliveira, ... Veterinary Parasitology: Regional Studies and Reports, 101354 , 2025 2025
Anticoagulant activity of Eucalyptus essential oils: An in vitro approach and a bioinformatics-based pharmacokinetic-pharmacodynamic analysis JF Dos Santos, MED Possate, MB de Almeida, EM de Melo, RA Furtado, ... Journal of Pharmacological and Toxicological Methods 135, 108381 , 2025 2025 Citations: 3
Correlation between quality of life of elderly people and presence of pets FLC Faggioni, LCG do Nascimento, DC Bulgo, S de Almeida Junior, ... Caderno Pedagógico 22 (8), e17389-e17389 , 2025 2025
Protective effect of omega-3 on reducing doxorubicin toxicity in preclinical trials MH de Paiva, S de Almeida-Junior, CB Garcia, PM Pereira, JG Martins, ... Toxicology Reports 14, 102056 , 2025 2025 Citations: 1
Analgesic and Anti‐Inflammatory Effects of Free and Nanoencapsulated Green Propolis Extract From the Northeastern Brazilian Caatinga Biome JA Aldana‐Mejia, M Rezende Granzoto, E Maria de Melo, V Pena Ribeiro, ... Chemistry & Biodiversity 22 (6), e202402896 , 2025 2025 Citations: 2
Therapeutic efficacy of brown propolis from Araucaria sp. in modulating rheumatoid arthritis PM Pereira, S de Almeida-Junior, NN de Melo Taveira, EM de Melo, ... Inflammopharmacology 33 (2), 799-807 , 2025 2025 Citations: 5
PRÓPOLIS MARROM BRASILEIRA CONTRA DOENÇA DE ALZHEIMER EM MODELO IN SILICO LB Callafatti, MS Tavares, JF dos Santos, RA Furtado, ... ARACÊ 6 (4), 13473-13490 , 2024 2024
Cryopreservation of canine ovarian tissue by slow freezing and vitrification: evaluation of follicular morphology and apoptosis rate NAL Stábile, FR de Oliveira, RA Furtado, CBML Felippe, MR Tavares, ... Theriogenology 230, 8-14 , 2024 2024 Citations: 9
P13-10 Exploring the therapeutic potential of solamargine in glioblastoma: an in vitro study DC Tavares, AB Ribeiro, MM Junqueira, MRS Melo, RA Furtado, ... Toxicology Letters 399, S213-S213 , 2024 2024
In vivo anti-inflammatory activity of BACCHARIN from BRAZILIAN green PROPOLIS S Almeida-Junior, KRP de Oliveira, LP Marques, JG Martins, H Ubeda, ... Fitoterapia 175, 105975 , 2024 2024 Citations: 9
Evaluation of the toxicogenetic potential of visceral leishmaniasis in naturally infected dogs and salivary samples for definitive disease diagnosis CO Alvarenga, RA Furtado, RP de Mendonça, L de Freitas Pereira, ... Cuadernos de Educación y Desarrollo-QUALIS A4 16 (4), e3868-e3868 , 2024 2024 Citations: 1
Evaluation of anti‐inflammatory activity of kaurenol: Experimental evaluation and mechanistic insights RA Furtado, SA Ross, S de Almeida Junior, RP de Mendonça, ... Fundamental & Clinical Pharmacology 38 (2), 252-261 , 2024 2024 Citations: 6
Uso do óleo essencial de hortelã pimenta associado à suplementação no desempenho de atletas do handebol masculino MC de Andrade, RA Furtado, FB Sarrassini, G Franco, MGM Pina Revista Brasileira de Nutriçao Esportiva 18 (112), 516-524 , 2024 2024
IDENTIFICAÇÃO DE TRANSTORNOS MENTAIS POR MEIO DE WINNIE-THE-POOH S de Almeida Junior Universidade de Franca , 2024 2024
Advances in the phytochemical screening and biological potential of propolis S de Almeida‐Junior, MVF Ferraz, AR de Oliveira, FP Maniglia, JK Bastos, ... Fundamental & Clinical Pharmacology 37 (5), 886-899 , 2023 2023 Citations: 15
Polyalthic Acid from Copaifera lucens Demonstrates Anticariogenic and Antiparasitic Properties for Safe Use MB Santiago, VCO Dos Santos, SC Teixeira, NBS Silva, PF de Oliveira, ... Pharmaceuticals 16 (10), 1357 , 2023 2023 Citations: 10
A review on the anti‐inflammatory activities of Brazilian green, brown and red propolis FF Dos Santos, RP Morais‐Urano, WR Cunha, SG de Almeida, ... Journal of food biochemistry 46 (10), e14350 , 2022 2022 Citations: 40
MOST CITED SCHOLAR PUBLICATIONS
Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration HGO Barud, HS Barud, M Cavicchioli, TS do Amaral, ... Carbohydrate Polymers 128, 41-51 , 2015 2015 Citations: 282
Antimutagenicity of rosmarinic acid in Swiss mice evaluated by the micronucleus assay MA Furtado, LCF de Almeida, RA Furtado, WR Cunha, DC Tavares Mutation Research/Genetic Toxicology and Environmental Mutagenesis 657 (2 … , 2008 2008 Citations: 153
Ursolic acid and oleanolic acid suppress preneoplastic lesions induced by 1, 2-dimethylhydrazine in rat colon RA Furtado, EP Rodrigues, FRR Araujo, WL Oliveira, MA Furtado, ... Toxicologic pathology 36 (4), 576-580 , 2008 2008 Citations: 119
In vivo analgesic and anti-inflammatory activities of Rosmarinus officinalis aqueous extracts, rosmarinic acid and its acetyl ester derivative R Lucarini, WA Bernardes, DS Ferreira, MG Tozatti, R Furtado, JK Bastos, ... Pharmaceutical Biology 51 (9), 1087-1090 , 2013 2013 Citations: 105
Protective effect of rosmarinic acid on V79 cells evaluated by the micronucleus and comet assays RA Furtado, FRR De Araujo, FA Resende, WR Cunha, DC Tavares Journal of Applied Toxicology: An International Journal 30 (3), 254-259 , 2010 2010 Citations: 91
Effectiveness of a back school program in low back pain LH Ribeiro, F Jennings, A Jones, R Furtado, J Natour Clinical & Experimental Rheumatology 26 (1), 81 , 2008 2008 Citations: 85
Antibacterial and antiproliferative activities of the fresh leaf essential oil of Psidium guajava L. (Myrtaceae) EAJ Silva, EBB Estevam, TS Silva, HD Nicolella, RA Furtado, CCF Alves, ... Brazilian Journal of Biology 79 (4), 697-702 , 2018 2018 Citations: 66
Chemopreventive effects of rosmarinic acid on rat colon carcinogenesis RA Furtado, BR Oliveira, LR Silva, SS Cleto, CC Munari, WR Cunha, ... European Journal of Cancer Prevention 24 (2), 106-112 , 2015 2015 Citations: 53
Chemopreventive effect of Copaifera langsdorffii leaves hydroalcoholic extract on 1,2-dimethylhydrazine-induced DNA damage and preneoplastic lesions in rat colon JM Senedese, JM Alves, IMS Lima, EAP de Andrade, RA Furtado, ... BMC complementary and alternative medicine 13 (1), 3 , 2013 2013 Citations: 53
I n vivo protective effect of Copaifera langsdorffii hydroalcoholic extract on micronuclei induction by doxorubicin JM Alves, CC Munari, M de Azevedo Bentes Monteiro Neto, RA Furtado, ... Journal of Applied Toxicology 33 (8), 854-860 , 2013 2013 Citations: 50
Antiproliferative activity of essential oils from three plants of the Brazilian Cerrado: Campomanesia adamantium (Myrtaceae), Protium ovatum (Burseraceae) and Cardiopetalum … CCF Alves, JD Oliveira, EBB Estevam, MN Xavier, HD Nicolella, ... Brazilian Journal of Biology 80 (2), 290-294 , 2019 2019 Citations: 47
Chemical composition and in vitro antibacterial and antiproliferative activities of the essential oil from the leaves of Psidium myrtoides O. Berg (Myrtaceae) ALB Dias, HRF Batista, EBB Estevam, CCF Alves, MR Forim, HD Nicolella, ... Natural product research 33 (17), 2566-2570 , 2019 2019 Citations: 45
18-Des-hydroxy Cytochalasin: an antiparasitic compound of Diaporthe phaseolorum -92C, an endophytic fungus isolated from Combretum lanceolatum Pohl ex … ER Brissow, IP da Silva, KA de Siqueira, JA Senabio, LP Pimenta, ... Parasitology research 116 (7), 1823-1830 , 2017 2017 Citations: 43
A review on the anti‐inflammatory activities of Brazilian green, brown and red propolis FF Dos Santos, RP Morais‐Urano, WR Cunha, SG de Almeida, ... Journal of food biochemistry 46 (10), e14350 , 2022 2022 Citations: 40
Assessment of genotoxic activity of oleoresins and leaves extracts of six Copaifera species for prediction of potential human risks RA Furtado, PF de Oliveira, JM Senedese, SD Ozelin, LDR de Souza, ... Journal of Ethnopharmacology 221, 119-125 , 2018 2018 Citations: 38
Flavonoids and Methoxy-galloylquinic Acid Derivatives from the Leaf Extract of Copaifera langsdorffii Desf. MS Nogueira, RA Furtado, JK Bastos Journal of agricultural and food chemistry 63 (31), 6939-6945 , 2015 2015 Citations: 38
Lipophilic gold (I) complexes with 1, 3, 4-oxadiazol-2-thione or 1, 3-thiazolidine-2-thione moieties: synthesis and their cytotoxic and antimicrobial activities AM de Almeida, BA de Oliveira, PP de Castro, CC de Mendonça, ... Biometals 30 (6), 841-857 , 2017 2017 Citations: 37
Effect of the dibenzylbutyrolactone lignan (−)-hinokinin on doxorubicin and methyl methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts FA Resende, IM Tomazella, LC Barbosa, M Ponce, RA Furtado, ... Mutation Research/Genetic Toxicology and Environmental Mutagenesis 700 (1-2 … , 2010 2010 Citations: 33
COVID-19 e a infecção por SARS-CoV-2 em um panorama geral/COVID-19 and infection by SARS-CoV-2 in an overview S de Almeida Júnior, RCOM Kairala, AG Pereira, GB da Costa, RCR Cruz, ... Brazilian Journal of Health Review 3 (2), 3508-3522 , 2020 2020 Citations: 32
Chemopreventive role of Copaifera reticulata Ducke oleoresin in colon carcinogenesis JM Senedese, F Rinaldi-Neto, RA Furtado, HD Nicollela, LDR de Souza, ... Biomedicine & Pharmacotherapy 111, 331-337 , 2019 2019 Citations: 31
