Genome-wide identification and characterization of Subtilisin-like Serine protease encoding genes in Vigna radiata L. Wilczek Poornima Singh, Brijesh Pandey, Shalini Purwar, Ramakrishnan Madhavan Nair, Aditya Pratap Scientific Reports, 2025 Subtilisin-like serine proteases (SBTs) are serine proteolytic enzymes that play various roles in plant growth, function and stress responses. Vigna glabrescens, a wild relative of mungbean known to be a potential donor of photo- and thermoperiod insensitivity, was characterized for thermotolerance through reproductive biology and gene expression profiling. Whole-genome sequencing of this species has not yet been performed; hence, genome-wide analysis of this species has not been explored. In the present study, a systematic analysis of SBT-encoding genes in the V. radiata (Vradi_SBT) genome was conducted, with a focus on their response during flower development under different temperature regimes, such as optimum temperature, heat and cold stresses, in Vigna radiata and a wild relative, Vigna glabrescens. Thirty-eight Vradi_SBT genes were identified in the V. radiata genome and were further grouped into five distinct subgroups. The key domain of the SBT peptidase, "peptidase_S8_53," was found in all 38 Vradi_SBT proteins, while 28 of them contained the "peptidase_S8" domain. Additionally, 30 of these proteins contained a maximum of 10 motifs. A total of 22 orthologous genes were identified in Arabidopsis thaliana, whereas paralogous gene pairs were detected as tandemly duplicated genes with V. unguiculata. Cis-acting element analysis revealed that these genes presented more stress-responsive promoter sequences than the other promoters. Furthermore, Vradi_SBT-1.9 was found significantly upregulated under both high- and low-temperature stresses. This study provides insights into SBT-encoding genes and their possible role in flower development and thermotolerance in Vigna species.
Exogenous application of γ-aminobutyric acid alleviates temperature stress in mungbean (Vigna radiata) and its wild non-progenitor (Vigna glabrescens) by regulating heat shock protein genes Poornima Singh, Brijesh Pandey, Shalini Purwar, Chandra Mohan Singh, Aditya Pratap, Awdhesh Kumar Mishra BMC Plant Biology, 2025 Abiotic stresses significantly affect plant growth and productivity. Identification of stress-tolerant genotypes is the best and an effective mitigation strategy. The present study evaluates the thermosensitive Vigna radiata cv. Shikha and the thermotolerant Vigna glabrescens accession TCR-20 under the control (non stress), cold stress, and heat stress conditions, without any treatment or foliar treatment with indole-3-acetic acid (IAA), salicylic acid (SA), or gamma-aminobutyric acid (GABA). Chlorophyll content revealed that TCR-20 maintained higher chlorophyll content under stress, whereas Shikha exhibited higher chlorophyll content upon foliar spray of GABA. Histochemical staining confirmed an increased oxidative stress under extreme temperatures, with GABA effectively mitigated the hydrogenperoxide and superoxide accumulation in both genotypes. Further, mining and comparative analysis of 96 heat shock proteins (HSPs), including HSP20, HSP60, HSP70, HSP90, and HSP100 were also done. Physicochemical characterization revealed varied stability, solubility, and thermostability of these proteins, which exhibited higher stress tolerance potential. All 96 HSPs were found widespread across the 11 chromosomes. Notably, the HSP70 family, particularly VrHSP-70.2 in TCR-20, exhibited the most robust response under both cold and heat stress, with significant upregulation, especially with GABA followed by IAA treatments. The genes such as VrHSP-70.2, VrHSP-60.22, and VrHSP-20.24 highlighted their significant upregulations in TCR-20 over Shikha. Overall, these findings provide valuable insights into the molecular and physiological mechanisms underlying thermotolerance in Vigna species, emphasizing the role of HSPs and stress-mitigating treatments for improving stress resilience in Vigna crops.
Screening of Metabolites and Metabolic Pathways in Five Different Ocimum Species From the Same Origin Using GC-MS Ravi Prakash Jaiswal, Vishal Chugh, Sushil Nagar, Shalini Purwar, Akbare Azam, Ankit Verma Biochemistry Research International, 2025 This study presents the comparative and preliminary phytochemical analysis of essential oils extracted from 5 different Ocimum species, including Ocimum basilicum Linn, Ocimum canum Sims, Ocimum citriodorum, Ocimum gratissimum Linn and Ocimum sanctum Linn. The gas chromatography coupled with single quadrupole mass spectrometry was employed for the screening of the different metabolites. The present study investigates a total number of 111 bioactive compounds which were identified across the five Ocimum species, with O. citriodorum exhibiting the highest diversity. The analysis revealed significant variations in the chemical profiles, attributed to differing eco‐climatic conditions. Key bioactive compounds, such as α‐pinene, linalool and caryophyllene, were consistently found across species. The study also mapped these compounds to metabolic pathways, highlighting their roles in monoterpenoid, phenylpropanoid and sesquiterpenoid biosynthesis. Detailed analysis of O. basilicum, O. citriodorum, O. sanctum, O. canum and O. gratissimum oils demonstrated unique metabolic properties, with each species showing distinct pathway activations and dominant compounds. The findings were validated using principal component analysis and hierarchical clustering analysis, confirming the species’ chemical diversity and distinct clustering. This comprehensive characterization enhances our understanding of Ocimum spp. essential oils, offering valuable insights for their pharmaceutical, food and cosmetic applications.
Tissue culture's role in advancing vegetable crop genetics: Pre-breeding and distant hybridization Tissue Culture Techniques in Vegetable Crop Improvement, 2024
Structural and Functional Diversity of Soil Microbes in Response to Climate Change in Forest Ecosystem Soil Plant and Environmental Nexus Volume 01 Soil Health Management for Sustainable Development Goals, 2024
Antioxidant and anti-diabetic potential of rebaudioside A and a mixture of steviol glycosides in alloxan-induced diabetic rats Indian Journal of Natural Products and Resources, 2021
Assessment of genetic diversity among fenugreek (Trigonella foenum-graecum L.), using RAPD molecular markers Journal of Medicinal Plants Research, 2011
Effect of jasmonic acid on growth characteristics of calli derived from mature embryos of wheat cultivars showing differential resistance to Karnal bunt (Tilletia indica) Plant Cell Biotechnology and Molecular Biology, 2006
Chemo-Diversity Landscape Using Physico-Biochemical, Elementals, and Metabolic Profiling in Different Stages and Accessions of Madhuca longifolia Flowers for … S Purwar, A Verma, RP Jaiswal, V Mishra, V Chugh, CM Singh, A Azam, ... Preprints , 2026 2026
Multi-and Meta-omics Approaches to Unlock the Complexity of Functional Traits in Legumes S Purwar, S Yadav, A Maurya, S Gupta, CM Singh Meta-omics in Crop Improvement: Volume II: Applications and Implications, 67-103 , 2026 2026
Exogenous application of γ-aminobutyric acid alleviates temperature stress in mungbean (Vigna radiata) and its wild non-progenitor (Vigna glabrescens) by regulating heat shock … P Singh, B Pandey, S Purwar, CM Singh, A Pratap, AK Mishra BMC Plant Biology 25 (1), 1651 , 2025 2025 Citations: 1
MAHUA ( Madhuca indica ): The Multifunctional Tree of Tradition and Sustenance S Purwar, V Bisht, E Gupta Fasting Superfoods: Cultivation, Nutrition & Market Potential, 329-357 , 2025 2025
Physiological and Biochemical Responses of Chrysanthemum× morifolium to Salinity Stress under In-vitro and in vivo Conditions R Mishra, R Kumar, A Gupta, V Chugh, AK Singh, SK Jawla, S Purwar 2025
R-Genes for improving disease and Insect-Pest resistance in legumes S Purwar, P Singh, B Pandey, V Chugh, Chirag, S Kashyap, P Kaundal, ... Integrated improvement of food legumes, 193-224 , 2025 2025 Citations: 1
Plant Protease Inhibitors Towards Modulating Resistance Response to Insect-Pests in Legumes V Chugh, V Mishra, S Purwar, AK Singh Integrated Improvement of Food Legumes, 77-99 , 2025 2025
Physiological and biochemical adaptations for salt stress tolerance in eggplant ( Solanum melongena ) A Fiza, V Chugh, AC Mishra, V Mishra, S Purwar, SV Dwivedi, A Mishra, ... Plant Physiology Reports 30 (2), 352-368 , 2025 2025 Citations: 8
Genome-wide identification and characterization of Subtilisin-like Serine protease encoding genes in Vigna radiata L. Wilczek P Singh, B Pandey, S Purwar, RM Nair, A Pratap Scientific Reports 15 (1), 13284 , 2025 2025 Citations: 2
Genome-wide identification and characterization of PGIP gene family in Vigna radiata L. Wilczek and its expression in wild non-progenitor, Vigna umbellata L … R Kumar, R Pandey, S Purwar, MK Mishra, A Rai, CM Singh Journal of Plant Biochemistry and Biotechnology 34 (1), 224-236 , 2025 2025 Citations: 3
Screening of Metabolites and Metabolic Pathways in Five Different Ocimum Species From the Same Origin Using GC‐MS RP Jaiswal, V Chugh, S Nagar, S Purwar, A Azam, A Verma Biochemistry Research International 2025 (1), 7121687 , 2025 2025 Citations: 2
Salicylic Acid-Induced Expression Profiles of LRR and LRR-RLK Candidate Genes Modulate Mungbean Yellow Mosaic India Virus Resistance in Blackgram and Its … M Shukla, P Kaundal, S Purwar, M Kumar, C Maurya, Chirag, AK Mishra, ... Plants 13 (24), 3601 , 2024 2024 Citations: 1
DUS Characterization in Lentil (Lens culinaris Medik) using Morphological Descriptors ASSPS 4. Dharm Veer Singh, Vijay Pratap, Kamaluddin, Shalini Purwar ... Journal of Advances in Biology & Biotechnology. 27, 167-177 , 2024 2024
Identification and characterization of potential sources of waterlogging tolerance in Vigna species as revealed by physiological, biochemical and gene expression … S Kumar, CM Singh, M Kumar, S Purwar, H Kumar, A Mishra, A Rai, ... Journal of Plant Biochemistry and Biotechnology 33 (4), 657-673 , 2024 2024 Citations: 2
In silico profiling, docking analysis, and protein interactions of secondary metabolites in Musa spp. Against the SGE1 protein of Fusarium oxysporum f. sp. cubense P Sonkar, S Purwar, P Bhargva, RP Singh, J Alkahtani, A Al-Hashimi, ... Computational Biology and Chemistry 113, 108230 , 2024 2024 Citations: 5
Adaptations of psychrophilic microorganism to low-temperature environments S Purwar, S Srivastava Applied Microbiology: Theory & Technology, 168-188 , 2024 2024 Citations: 6
Comprehensive characterization of protease inhibiting gene family, cis -regulatory elements, and protein interaction network in linseed and their expression upon … CM Singh, BK Singh, S Purwar, B Nair, Ruchi, A Patel, S Singh, V Kaur Scientific Reports 14 (1), 17907 , 2024 2024 Citations: 2
Genome-wide identification and analysis of Subtilisin-like serine protease gene family in banana ( Musa accuminta L.) and their expression under abiotic stresses S Purwar, V Chugh, P Singh, AK Srivastava, AK Singh, AC Mishra, ... Plant Biotechnology Reports 18 (1), 143-160 , 2024 2024 Citations: 6
Article Not peer-reviewed version AB Porcaro, A Bianchi, S Gallina, E Serafin, S Costantino, F Montanaro, ... 2024
Tissue culture's role in advancing vegetable crop genetics: Pre-breeding and distant hybridization. AP Shalini Purwar, Kapil Gupta, Poornima Singh, Jyoti Singh, Ira Sharma ... Tissue Culture Techniques in Vegetable Crop Improvement. https://doi.org/10 … , 2024 2024
MOST CITED SCHOLAR PUBLICATIONS
Nutritional and therapeutic values of Stevia rebaudiana: A review E Gupta, S Purwar, S Sundaram, GK Rai Journal of Medicinal Plants Research 7 (46), 3343-3353 , 2013 2013 Citations: 306
Potential applications of food derived bioactive peptides in management of health A Dhaval, N Yadav, S Purwar International Journal of Peptide Research and Therapeutics 22 (3), 377-398 , 2016 2016 Citations: 79
Improving Drought Tolerance in Mungbean ( Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives CM Singh, P Singh, C Tiwari, S Purwar, M Kumar, A Pratap, S Singh, ... Agronomy 11 (8), 1534 , 2021 2021 Citations: 66
Anticancer potential of steviol in MCF-7 human breast cancer cells E Gupta, S Kaushik, S Purwar, R Sharma, AK Balapure, S Sundaram Pharmacognosy Magazine 13 (51), 345 , 2017 2017 Citations: 65
Nutritional and medicinal potential of Grewia subinaequalis DC.(syn. G. asiatica.)(Phalsa) J Sinha, S Purwar, SK Chuhan, G Rai Journal of Medicinal Plants Research 9 (19), 594-612 , 2015 2015 Citations: 62
Withania som nifera (Ashvvagandha) to Bacterial Pathogens S Sundaram, P Dwivedi, S Purwar Asian Journal of Biotechnology 3 (2), 194-199 , 2011 2011 Citations: 60
Stevioside and rebaudioside A-predominant ent-kaurene diterpene glycosides of therapeutic potential: a review E Gupta, S Purwar, S Sundaram, P Tripathi, G Rai Czech Journal of Food Sciences 34 (4), 281-299 , 2016 2016 Citations: 58
Assessment of genetic diversity among fenugreek (Trigonella foenum-graecum L.), using RAPD molecular markers S Sundaram, S Purwar Journal of Medicinal Plants Research 5 (9), 1543-1548 , 2011 2011 Citations: 48
Applications of molecular markers for developing abiotic-stress-resilient oilseed crops V Chugh, D Kaur, S Purwar, P Kaushik, V Sharma, H Kumar, A Rai, ... Life 13 (1), 88 , 2022 2022 Citations: 39
Breeding for enhancing Legumovirus resistance in mungbean: Current understanding and future directions CM Singh, P Singh, A Pratap, R Pandey, S Purwar, CA Douglas, KH Baek, ... Agronomy 9 (10), 622 , 2019 2019 Citations: 39
Sensory evaluation and nutritional composition of developed papaya-gooseberry jam E Gupta, S Purwar, P Jaiswal, R Chaturvedi, GK Rai Food and Nutrition Sciences 7 (7), 600-608 , 2016 2016 Citations: 35
Influence of jasmonic acid as potential activator of induced resistance against Karnal bunt in developing spikes of wheat MK Mandal, D Pandey, S Purwar, US Singh, A Kumar Journal of biosciences 31 (5), 607-616 , 2006 2006 Citations: 34
Antibacterial activities of crude extracts of Chlorophytum borivilianum to bacterial pathogens S Sundaram, P Dwivedi, S Purwar Research Journal of Medicinal Plant 5 (3), 343-347 , 2011 2011 Citations: 33
Enzymes of phenylpropanoid metabolism involved in strengthening the structural barrier for providing genotype and stage dependent resistance to karnal bunt in wheat S Purwar, SM Gupta, A Kumar American Journal of Plant Sciences 3 (2), 261-267 , 2012 2012 Citations: 31
Chironji (Buchanania lanzan) Wonder Tree: Nutritional and Therapeutic Values VBSP Neeraj International Journal of Current Microbiology and Applied Sciences ISSN … , 2020 2020 Citations: 29
Induction of a wheat β-1,3-glucanase gene during the defense response to Bipolaris sorokiniana R Aggarwal, S Purwar, L Kharbikar, S Gupta Acta Phytopathologica et Entomologica Hungarica 46 (1), 39-47 , 2011 2011 Citations: 29
Basal expression studies of cystatins during specific growth stages of wheat spikes for defining their possible role in differential and stage dependent immunity against Karnal … S Purwar, SS Marla, US Singh, A Kumar Molecular biology reports 37 (3), 1377-1389 , 2010 2010 Citations: 17
Expression and in silico characterization of Phenylalanine ammonium lyase against karnal bunt (Tilletia indica) in wheat (Triticum aestivum) S Purwar, S Sundaram, S Sinha, A Gupta, N Dobriyall, A Kumar Bioinformation 9 (20), 1013 , 2013 2013 Citations: 15
Genome-Wide Identification and Analysis of NBS-LRR -Encoding Genes in Mungbean ( Vigna radiata L. Wilczek) and Their Expression in Two Wild Non-progenitors Reveal … S Purwar, CM Singh, M Kumar, AK Singh, A Pratap, P Singh, PG Gore, ... Journal of Plant Growth Regulation 42 (10), 6667-6680 , 2023 2023 Citations: 14
Evaluation of Nutritional, Anti-Nutritional and Bioactive Compounds in Juice and Powder of Stevia rebaudiana E Gupta, S Purwar, A Singh, S Sundaram, GK Rai, S Sundaram Indian Journal of Natural Sciences 5 (28), 3308-3317 , 2015 2015 Citations: 14
