Incidence and risk factors associated with human mastitis Pratiksha Gondkar, Hemant Kumar, Khushal Patel Health Sciences Review, 2024 Human mastitis is an infection that affects the mammary glands of females and mainly occurs during the puerperium, a common condition brought on by improper breastfeeding technique. Mastitis is differentiated into lactational and non-lactational types according to its symptoms. Based on the available data, the pooled incidence of mastitis across countries like the USA, UK, Australia, Denmark, Turkey, Finland and New Zealand is estimated to be 13.45%. Poor hygiene, sore and cracked nipples, nipple piercing, smoking and fitted innerwear are reported risk factors. Staphylococcus aureus and Staphylococcus epidermidis are predominant pathogens, with a prevalence range between 50 to 87%, respectively. Mastitis management is essential to reduce infection rates and discover alternate treatments. Studies indicate that antibiotic treatment may be substituted for herbal and probiotic therapies. In addition, if mastitis is not adequately diagnosed in females, there is a high risk of breast cancer. Mastitis is a significant health issue, but it is often overlooked in the field of human medicine. In this review, we have focused on the risk factors, the alternative therapeutic approaches, and the relationship of breast cancer associated with mastitis.
Isolation, Cytotoxicity, and In-silico Screening of Coumarins from Psoralea corylifolia Linn. Nancy Tripathi, Aarati Parmar, Nilesh Pandey, Nivedita Bhardwaj, Sanheeta Chakrabarty, Ruma Sarkar, Hemant Kumar, Shreyans K. Jain Chemistry and Biodiversity, 2024 Psoralea corylifolia (syn. Cullen corylifolium), commonly called bawachi, is a medicinal plant extensively used for skin conditions like leukoderma, vitiligo, and psoriasis. It is notably rich in valuable bioactive compounds, particularly coumarins and furanocoumarins. This study isolated fourteen coumarins from P. corylifolia which were tested for cytotoxicity using the MTT assay, with compound 10 showing good cytotoxicity against A549 cells (IC50 0.9 μM), while compound 1, compound 2, and compound 3 displaying potential cytotoxicity against MDA‐MB‐231 cells (IC50 0.49 μM, 0.56 μM, and 0.84 μM respectively). Additionally, the compounds′ interaction with Epidermal Growth Factor Receptor (EGFR) protein, highly expressed in both cell lines, was investigated through molecular modeling studies, that aligned well with cytotoxicity results. The findings revealed the remarkable cytotoxic potential of four coumarins 1, 2, 3, and 10 against A549 and MDA‐MB‐231 cell lines.
Antibacterial Activity of Ampelocissus latifolia Tuberous Root Extract against Diverse Microbes Kamal Kishor Rajak, Pavan M. Pahilani, Hemant Kumar Asian Journal of Chemistry, 2023 Microbial drug resistance is growing every day and novel drugs are need of the hour to counter the future threat of large pandemic. The present work for the first time explores the antibacterial activity of methanol, hydro-alcoholic and n-hexane extracts of Ampelocissus latifolia (A. latifolia) tuberous root against few laboratory and clinical isolates. A total of 10 microbes were screened; out of which 1 is a laboratory strain (acid-fast) and the rest 9 are clinical isolates (5 Gram-positive and 4 are Gram-negative), 2 clinical isolates were drug-resistant (1 Gram-positive and 1 Gram-negative). In this screening, methanol extract showed potential antibacterial activity against Gram-positive, Gram-negative and acid-fast bacteria with zones of inhibition measuring 18 mm, 10 mm and 8 mm, respectively. Hydro-alcoholic and n-hexane extract showed a smaller zone of inhibition compared to methanol extract and the positive control. The minimum inhibitory concentration (MIC) of methanol and the hydro-alcoholic extract of powdered tuber root was determined by the micro-broth dilution method. The minimum inhibitory concentration of methanol and hydro-alcoholic extract for Gram-positive and Gram-negative microbes varies from 0.18 mg/mL to 1.5 mg/mL. The methanol and hydro-alcoholic extracts were analyzed with FTIR, which revealed the presence of alcohol, alkanes, alkenes, alkyls, ketones and nitro groups in the methanol and hydro-alcoholic extracts of A. latifolia tuberous root. The high-performance thin-layer chromatography (HPTLC) analysis of the methanol extract indicates the presence of at least 5 different compounds with different Rf values.
Virtual screening and molecular simulation study of natural products database for lead identification of novel coronavirus main protease inhibitors Nancy Tripathi, Bharat Goel, Nivedita Bhardwaj, Bharat Sahu, Hemant Kumar, Shreyans K. Jain Journal of Biomolecular Structure and Dynamics, 2022 3CL like protease (3CLpro or Mpro) is one of the main proteases of 2019-nCoV. The 3CLpro is a nonstructural protein of SARS-CoV and has an essential role in viral replication and transcription, thus, could be a potential target for anti-SARS drug development. The present study employed ligand- and structure-based approaches to identify the potent inhibitors of 2019-nCoV protease. The e-pharmacophore developed from 3CLpro-1 yielded virtual hits, that were subjected through drug likeliness and PAINS filters to remove interfering compounds. Further comprehensive docking studies, free energy calculations and ADMET studies resulted in two virtual leads- MolPort-000-410-348 and MolPort-002-530-156. The compounds MolPort-000-410-348 and MolPort-002-530-156 displayed good docking score of -12.09 and -13.38 Kcal/mol and free binding energy of -63.34 ± 2.03 and -61.52 ± 2.24 Kcal/mol, respectively. The compounds also exhibited satisfactory predicted ADMET profile and were subjected to molecular dynamic (MD) studies. The MD simulation produced stable complexes of these ligands with 3CLpro protein and ligand RMSD in acceptable limits. Communicated by Ramaswamy H. Sarma.
Retraction: Withdrawal: A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant (Journal of Biological Chemistry (2010) 285 (26532-26544) DOI: 10.1074/jbc.M110.111021) Vikas Yadav, Manoj Kumar, Deepak Kumar Deep, Hemant Kumar, Ruby Sharma, Takshashila Tripathi, Narendra Tuteja, Ajay Kumar Saxena, Atul Kumar Johri Journal of Biological Chemistry, 2021 VOLUME 285 (2010) PAGES 26532–26544 This article has been withdrawn by the authors except Ajay Kumar Saxena and Deepak Kumar Deep who could not be contacted. Fig. 5C panel 3 is duplicated in Fig. 10A, b iii. Fig. 10A, a iii is duplicated as Fig. 1 in Microbiology (2009), 155: 780–790. Fig. 5C panel 6 is duplicated as Fig. 1A, b ii right, Fig. 10A, a iii is duplicated as Fig. 1A, a ii right, and Fig. 10A, c is duplicated in Fig. 1A, d in Plant Signaling & Behavior (2011) 6(5): 723–725, respectively representing different experimental conditions. Plant Signaling & Behavior as well as Microbiology have replaced and corrected the figures which were overlapping with the JBC figures as mentioned above. Both Plant Signaling & Behavior and Microbiology have published a corrigendum related to the respective figures. A Phosphate Transporter from the Root Endophytic Fungus Piriformospora indica Plays a Role in Phosphate Transport to the Host PlantJournal of Biological ChemistryVol. 285Issue 34PreviewBecause pure cultures and a stable transformation system are not available for arbuscular mycorrhizal fungi, the role of their phosphate transporters for the symbiotic interaction with the plant up till now could not be studied. Here we report the cloning and the functional analysis of a gene encoding a phosphate transporter (PiPT) from the root endophytic fungus Piriformospora indica, which can be grown axenically. The PiPT polypeptide belongs to the major facilitator superfamily. Homology modeling reveals that PiPT exhibits twelve transmembrane helices divided into two halves connected by a large hydrophilic loop in the middle. Full-Text PDF Open Access
Crystal structure of a ligand-bound LacY–Nanobody complex Hemant Kumar, Janet S. Finer-Moore, Xiaoxu Jiang, Irina Smirnova, Vladimir Kasho, Els Pardon, Jan Steyaert, H. Ronald Kaback, Robert M. Stroud Proceedings of the National Academy of Sciences of the United States of America, 2018
Structure of sugar-bound LacY Hemant Kumar, Vladimir Kasho, Irina Smirnova, Janet S. Finer-Moore, H. Ronald Kaback, Robert M. Stroud Proceedings of the National Academy of Sciences of the United States of America, 2014
Crystal structure of a eukaryotic phosphate transporter Bjørn P. Pedersen, Hemant Kumar, Andrew B. Waight, Aaron J. Risenmay, Zygy Roe-Zurz, Bryant H. Chau, Avner Schlessinger, Massimiliano Bonomi, William Harries, Andrej Sali, Atul K. Johri, Robert M. Stroud Nature, 2013
Novel anti-NS1 human single domain antibody from dengue infected NS1 positive patients OM Bhikhabhai, H Kumar Protein Expression and Purification, 106875 , 2025 2025
Methods for Identifying Candida albicans in the Sputum of Pneumonia Patients D Baidya, K Patel, P Gondkar, A Tyagi, DH Patel, H Kumar Indian Journal of Natural Sciences 16 (92), 101451-101464 , 2025 2025
Challenges in Devising a Diagnostic Approach for Idiopathic Conditions Surging to Pandemic Situations D Baidya, K Patel, H Kumar, K Panchal Novel Diagnostic Methods in Clinical Microbiology 3, 485-503 , 2025 2025
Gardenin A alleviates alcohol-induced oxidative stress and inflammation in HepG2 and Caco2 cells via AMPK/Nrf2 pathway P Chadha, H Aghara, D Johnson, D Sharma, M Odedara, M Patel, ... Bioorganic Chemistry 161, 108543 , 2025 2025 Citations: 8
Evaluating the Effect of Aeration on Growth Conditions for Commercial Baker’s Yeast: A Comparative Study on Batch Fermentation Processes with Saccharomyces cerevisiae PS Ukani, P Gondkar, D Baidya, K Patel, DH Patel, H Kumar Indian Journal of Natural Sciences 14 (82), 69241-69248 , 2024 2024
Correction to: Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques JH Bera, H Kumar, N Pandey, DN Patel Archives of Microbiology 206 (12), 454 , 2024 2024
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica H Kumar, A Bajaj, P Kumar, R Aggarwal, V Chalia, RK Pradhan, R Yadav, ... Protein Expression and Purification 223, 106559 , 2024 2024 Citations: 2
Incidence and risk factors associated with human mastitis P Gondkar, H Kumar, K Patel Health Sciences Review 12, 100191 , 2024 2024 Citations: 12
Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques JH Bera, LS Raj. A, H Kumar, N Pandey, DN Patel Archives of Microbiology 206 (9), 369 , 2024 2024 Citations: 3
Isolation, Cytotoxicity, and In‐silico Screening of Coumarins from Psoralea corylifolia Linn. N Tripathi, A Parmar, N Pandey, N Bhardwaj, S Chakrabarty, R Sarkar, ... Chemistry & Biodiversity 21 (2), e202301841 , 2024 2024 Citations: 8
Green synthesis of silver nanoparticles using Curcuma longa flower extract and antibacterial activity K Kishor Rajak, P Pahilani, H Patel, B Kikani, R Desai, H Kumar arXiv e-prints, arXiv: 2304.04777 , 2023 2023
Identification and Characterization of Anti-mycobacterial Compound from Piper nigrum HK Kamal Kishor Rajak#, Odedara Mitalben Bhikhabhai#, Sanheeta Chakrabarty ... Indian Journal of Natural Sciences, 14 (82), 69249-69258 , 2023 2023
Antibacterial Activity of Ampelocissus latifolia Tuberous Root Extract against Diverse Microbes HK Kamal Kishor Rajak, Pavan M Pahilani Asian Journal of Chemistry 35 (08) , 2023 2023 Citations: 4
Virtual screening and molecular simulation study of natural products database for lead identification of novel coronavirus main protease inhibitors N Tripathi, B Goel, N Bhardwaj, B Sahu, H Kumar, SK Jain Journal of Biomolecular Structure and Dynamics 40 (8), 3655-3667 , 2022 2022 Citations: 28
Diversity in kinetics correlated with structure in nano body-stabilized LacY H Kumar, J Finer-Moore, I Smirnova, V Kasho, E Pardon, J Steyaert, ... PLoS One 15 (5), e0232846 , 2020 2020 Citations: 3
Crystal structure of a ligand-bound LacY–Nanobody complex H Kumar, JS Finer-Moore, X Jiang, I Smirnova, V Kasho, E Pardon, ... Proceedings of the National Academy of Sciences 115 (35), 8769-8774 , 2018 2018 Citations: 40
Lactose Permease: Mechanism through Structures H Kumar, HR Kaback, RM Stroud Biophysical Journal 114 (3), 333a , 2018 2018
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site H Kumar, JS Finer-Moore, HR Kaback, RM Stroud Proceedings of the National Academy of Sciences 112 (29), 9004-9009 , 2015 2015 Citations: 58
Structure of sugar-bound LacY H Kumar, V Kasho, I Smirnova, JS Finer-Moore, HR Kaback, RM Stroud Proceedings of the National Academy of Sciences 111 (5), 1784-1788 , 2014 2014 Citations: 133
Crystal structure of a eukaryotic phosphate transporter BP Pedersen, H Kumar, AB Waight, AJ Risenmay, Z Roe-Zurz, BH Chau, ... Nature 496 (7446), 533-536 , 2013 2013 Citations: 280
MOST CITED SCHOLAR PUBLICATIONS
A phosphate transporter from the root endophytic fungus Piriformospora indica plays a role in phosphate transport to the host plant V Yadav, M Kumar, DK Deep, H Kumar, R Sharma, T Tripathi, N Tuteja, ... Journal of Biological Chemistry 285 (34), 26532-26544 , 2010 2010 Citations: 407
Crystal structure of a eukaryotic phosphate transporter BP Pedersen, H Kumar, AB Waight, AJ Risenmay, Z Roe-Zurz, BH Chau, ... Nature 496 (7446), 533-536 , 2013 2013 Citations: 280
Structure of sugar-bound LacY H Kumar, V Kasho, I Smirnova, JS Finer-Moore, HR Kaback, RM Stroud Proceedings of the National Academy of Sciences 111 (5), 1784-1788 , 2014 2014 Citations: 133
Piriformospora indica enhances plant growth by transferring phosphate M Kumar, V Yadav, H Kumar, R Sharma, A Singh, N Tuteja, AK Johri Plant signaling & behavior 6 (5), 723-725 , 2011 2011 Citations: 111
Role of pilus proteins in adherence and invasion of Streptococcus agalactiae to the lung and cervical epithelial cells P Sharma, H Lata, DK Arya, AK Kashyap, H Kumar, M Dua, A Ali, AK Johri Journal of Biological Chemistry 288 (6), 4023-4034 , 2013 2013 Citations: 75
Structure of LacY with an α-substituted galactoside: Connecting the binding site to the protonation site H Kumar, JS Finer-Moore, HR Kaback, RM Stroud Proceedings of the National Academy of Sciences 112 (29), 9004-9009 , 2015 2015 Citations: 58
Crystal structure of a ligand-bound LacY–Nanobody complex H Kumar, JS Finer-Moore, X Jiang, I Smirnova, V Kasho, E Pardon, ... Proceedings of the National Academy of Sciences 115 (35), 8769-8774 , 2018 2018 Citations: 40
Virtual screening and molecular simulation study of natural products database for lead identification of novel coronavirus main protease inhibitors N Tripathi, B Goel, N Bhardwaj, B Sahu, H Kumar, SK Jain Journal of Biomolecular Structure and Dynamics 40 (8), 3655-3667 , 2022 2022 Citations: 28
Incidence and risk factors associated with human mastitis P Gondkar, H Kumar, K Patel Health Sciences Review 12, 100191 , 2024 2024 Citations: 12
Gardenin A alleviates alcohol-induced oxidative stress and inflammation in HepG2 and Caco2 cells via AMPK/Nrf2 pathway P Chadha, H Aghara, D Johnson, D Sharma, M Odedara, M Patel, ... Bioorganic Chemistry 161, 108543 , 2025 2025 Citations: 8
Isolation, Cytotoxicity, and In‐silico Screening of Coumarins from Psoralea corylifolia Linn. N Tripathi, A Parmar, N Pandey, N Bhardwaj, S Chakrabarty, R Sarkar, ... Chemistry & Biodiversity 21 (2), e202301841 , 2024 2024 Citations: 8
Antibacterial Activity of Ampelocissus latifolia Tuberous Root Extract against Diverse Microbes HK Kamal Kishor Rajak, Pavan M Pahilani Asian Journal of Chemistry 35 (08) , 2023 2023 Citations: 4
Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques JH Bera, LS Raj. A, H Kumar, N Pandey, DN Patel Archives of Microbiology 206 (9), 369 , 2024 2024 Citations: 3
Diversity in kinetics correlated with structure in nano body-stabilized LacY H Kumar, J Finer-Moore, I Smirnova, V Kasho, E Pardon, J Steyaert, ... PLoS One 15 (5), e0232846 , 2020 2020 Citations: 3
Biochemical characterization of a high affinity phosphate transporter (PiPT) from root endophyte fungus Piriformospora indica H Kumar, A Bajaj, P Kumar, R Aggarwal, V Chalia, RK Pradhan, R Yadav, ... Protein Expression and Purification 223, 106559 , 2024 2024 Citations: 2
Novel anti-NS1 human single domain antibody from dengue infected NS1 positive patients OM Bhikhabhai, H Kumar Protein Expression and Purification, 106875 , 2025 2025
Methods for Identifying Candida albicans in the Sputum of Pneumonia Patients D Baidya, K Patel, P Gondkar, A Tyagi, DH Patel, H Kumar Indian Journal of Natural Sciences 16 (92), 101451-101464 , 2025 2025
Challenges in Devising a Diagnostic Approach for Idiopathic Conditions Surging to Pandemic Situations D Baidya, K Patel, H Kumar, K Panchal Novel Diagnostic Methods in Clinical Microbiology 3, 485-503 , 2025 2025
Evaluating the Effect of Aeration on Growth Conditions for Commercial Baker’s Yeast: A Comparative Study on Batch Fermentation Processes with Saccharomyces cerevisiae PS Ukani, P Gondkar, D Baidya, K Patel, DH Patel, H Kumar Indian Journal of Natural Sciences 14 (82), 69241-69248 , 2024 2024
Correction to: Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques JH Bera, H Kumar, N Pandey, DN Patel Archives of Microbiology 206 (12), 454 , 2024 2024
