Pollyana Christine Gomes Roque

@saomateus.ufes.br

Pos-doctoral fellow and Coordenação Universitária Norte do Espírito Santo (Ceunes)
Universidade Federal do Espírito Santo

Pollyana Christine Gomes Roque


               Download Compact PDF  
https://researchid.co/pollyanaroque

RESEARCH, TEACHING, or OTHER INTERESTS

Ecology, Animal Science and Zoology, Oceanography
11

Scopus Publications

Scopus Publications

  • Genetic Diversity and Population Structure of Squalus cf. mitsukurii (Chondrichthyes: Squalidae) Revealed by ddRAD Sequencing
    Aisni Mayumi Corrêa de Lima Adachi, Ailton Amarante Ariza, Giovana da Silva Ribeiro, Pollyana Christine Gomes Roque, Matheus Marcos Rotundo, Marcelo Vianna, Gabriela Delpiani, Sergio Matias Delpiani, Juan Martín Díaz de Astarloa, Claudio Oliveira, Fausto Foresti, Vanessa Paes da Cruz
    Animal Genetics, 2026
    Assessing genetic structure across ocean basins is essential to understand connectivity and guide conservation in data‐deficient open‐water sharks. In this study, we examined the population genomics of Squalus cf. mitsukurii by analyzing tissue samples collected from two distant regions: California, USA (Pacific Ocean) and Pernambuco, Brazil (Southwestern Atlantic Ocean). Using double‐digest restriction site‐associated DNA sequencing (ddRADseq), we generated 33 480 939 raw reads across 28 individuals and retained 1118 high‐quality single nucleotide polymorphisms (SNPs) after stringent filtering. Genetic diversity was moderate (He = 0.211–0.244), with low inbreeding levels ( F is = −0.005–0.106). DAPC and pairwise F st analyses revealed moderate genetic structure, indicating limited gene flow between the sampled populations. This study provides the first genomic evidence of population differentiation in S. cf. mitsukurii across these regions. Our findings underscore the importance of genome‐wide data and informing regionally conservation strategies, especially for exploited species commonly misidentified in commercial landings and lacking population management.
  • Differences in sagittal otoliths between two morid species: insights into the genus Physiculus (Moridae: Gadiformes)
    César Santificetur, Pollyana Christine Gomes Roque, Sara de Castro Loebens, Alessandra Maria Advíncula Pires, Danielle de Lima Viana, Paulo Guilherme Vasconcelos de Oliveira
    Neotropical Ichthyology, 2026
    Sagittae are widely used as tools in taxonomic and ecological studies of fishes, especially when external morphological characters are conserved or ambiguous. We analyzed morphological variation between two congeneric species of Physiculus: P. cirm, endemic to the Saint Peter and Saint Paul Archipelago, and P. kaupi, distributed along the Brazilian continental slope. Fifty adult specimens (25 per species) were examined using qualitative descriptions, morphometrics with shape indices, and 4th and 5th order wavelet contour decomposition. This work represents the first morphometric description and analysis of their sagittal otoliths. Both species exhibited sagitiform otoliths with a plane-convex profile and elliptical ostium, but the anterior region is more rounded in P. cirm and more peaked in P. kaupi, whereas the posterior region is lanceolate to rounded in P. cirm but lanceolate in P. kaupi. Statistically significant differences were found in otolith height/otolith length (OH/OL), otolith thickness/ otolith length (OT/OL), sulcus acusticus area/ otolith area (SA/OA), and otolith length/ total length (OL/TL). Classification using morphometric indices and 5th order wavelets achieved 98% accuracy, highlighting strong discriminative potential. These morphological differences likely reflect both taxonomic divergence and adaptation to deep-sea habitats, supporting otolith morphology for species identification and ecological inference in deep-sea fishes.
  • Biodiversity assessment using BRUVS in a port area of Pernambuco, northeast Brazil
    Edson Bortoletto Garciov-Filho, Andrey Paulo Cavalcanti Soares, Ilka Siqueira Lima Branco Nunes, Pollyana Christine Gomes Roque, Alessandra Fonseca Fischer, José Carlos Pacheco dos Santos, Natalia Priscila Alves Bezerra, Paulo Guilherme Vasconcelos de Oliveira
    Boletim do Instituto De Pesca, 2025
    Located in the second-largest estuary in Pernambuco, Brazil, the Suape Port Industrial Complex (CIPS) and its surroundings are regions of high environmental and economic importance that experience the effects of constant anthropogenic action. To understand the interference of these acts and the marine environment in this area, to identify its taxonomic diversity and abundance, and to guide management and mitigation measures for the fauna and marine ecosystem of this location, using the non-lethal baited remote underwater video system (BRUVS) method, between July 2021 and October 2022, we conducted a survey of the fauna in the coastal zone of the CIPS area, with a sampling area of 180 km2 and 800 sampling points. Among the 118 species identified, the most abundant were Haemulon aurolineatum, Decapterus macarellus, and Caranx crysos, with a greater association of individuals in the sand/gravel + phytobenthos substrate, sand/gravel, and limestone formation. Although no non-significant differences were observed in the diversity of species by lunar phase, the relative abundance was greater during the new moon. When evaluating the depth gradients, those with the highest relative abundance and diversity were observed in the extracts at 20–25 and 25–30 m.
  • Genetic Population Structure and Diversity of the Whitetail Dogfish Squalus albicaudus (Chondrichthyes, Squaliformes) along the Brazilian Coast as Identified by SNP Markers
    Aisni M. C. L. Adachi, Pollyana C. G. Roque, Fabio H. V. Hazin, Marcelo Vianna, Matheus M. Rotundo, Claudio Oliveira, Fausto Foresti, Vanessa P. Cruz
    Fishes, 2023
    The shark Squalus albicaudus, categorized by the International Union for Conservation of Nature red list as Data Deficient due to lack of minimal information for classification, is distributed throughout the Brazilian coast. High pressures such as overfishing and anthropic activities, as well as certain biological characteristics, including k strategists, comprise influential shark stocks reduction agents. However, genetic diversity, population structure, connectivity, and effective population size data are still limited for S. albicaudus, indicating the need for further studies. In this context, the genetic variability and population structure of S. albicaudus were investigated herein to test for panmixia. Samples were obtained from coasts of the Brazilian states of Pernambuco, Rio de Janeiro, and São Paulo along the species distribution range, and single nucleotide polymorphisms (SNPs) were assessed by the ddRADseq method. The findings revealed a panmitic S. albicaudus population, explained by certain life strategies, such as polyandry and migratory behavior. Based on the genomic findings reported herein, a single S. albicaudus population should be considered in the study area, indicating the need for specific management and conservation plans at the regional scale.
  • Abundance and diversity of deep-sea crustaceans (Decapoda and Isopoda) in the upper slope of state of Pernambuco–Brazil: With five new records
    Rômulo Costa Pires Ferreira, Danielle de Lima Viana, Paulo Guilherme Vasconcelos de Oliveira, Pollyana Christine Gomes Roque, Ilka Siqueira Lima Branco-Nunes, Alessandra Maria Advincula Pires, Diogo Martins Nunes, Fábio Hissa Vieira Hazin
    Regional Studies in Marine Science, 2023
  • Weak range-wide population structure in the blackfin tuna (Thunnus atlanticus) revealed by analysis of genome-wide SNPs
    Pavel Dimens, Lionel Reynal, Cedric Pau, Freddy Arocha, Fabio Hazin, Pollyana Roque, Nancie J Cummings, James S Franks, Kenneth L Jones, Eric A Saillant
    ICES Journal of Marine Science, 2023
    Blackfin tuna (Thunnus atlanticus) is a small tuna distributed in the western Atlantic Ocean where it is exploited by growing recreational and commercial regional fisheries. In this work, genome-wide genetic variation was analysed to investigate the occurrence of stock subdivision. A de novo assembly of the blackfin tuna genome was generated using Illumina paired-end sequencing data and applied as a reference for population genomic analysis of specimens from nine localities (average sample size per locality n = 72) spanning most of the blackfin tuna distribution range. A total of 2139 single-nucleotide polymorphisms were discovered and genotyped using the double-digest restriction associated DNA sequencing. Pairwise exact homogeneity tests were significant in 24 out of 36 population pairs and significant spatial autocorrelation of genotypes was observed for specimens collected within 2250 km of each other. However, divergence among locality samples was very low (pairwise FST range 0.0002–0.0025) and significant temporal variations were detected in localities sampled multiple times. Approaches to detect cryptic groups de novo were unsuccessful. Additional sampling is warranted to determine if multiple stocks need to be defined for management and assess temporal and spatial patterns of gene flow connecting them.
  • Population structure of blackfin tuna (Thunnus atlanticus) in the western Atlantic Ocean inferred from microsatellite loci
    Eric A. Saillant, Patricia L. Luque, Emily Short, Luca Antoni, Lionel Reynal, Cedric Pau, Freddy Arocha, Pollyana Roque, Fabio Hazin
    Scientific Reports, 2022
    The blackfin tuna,Thunnus atlanticus, is a small tropical tuna exploited by recreational and commercial fisheries in various parts of its range. Information on stock structure is needed to develop management plans for this species but is currently lacking. In this work, 470 blackfin tuna from nine geographic populations were assayed at 13 homologous microsatellite markers to provide a first assessment of stock structure across the species range. The overall divergence among locality samples was very low (overall FST = 0.0004) indicating high connectivity of blackfin tuna across their range. No clear grouping of localities in differentiated units was inferred but structuring followed a weak isolation by distance pattern (r = 0.16, P = 0.032). Pairwise exact tests and spatial analysis of molecular variance suggested divergence of the sample collected offshore Baía Formosa (Brazil) possibly reflecting reproductive isolation of Brazilian populations from those in the Caribbean region and further north. Further study of the status of Brazilian populations and the transition between this region and the Caribbean is warranted. Cryptic subdivision within the Northern Hemisphere part of the range is possible and should be evaluated using increased marker density and a more comprehensive geographic coverage.
  • DNA Barcoding and Species Delimitation for Dogfish Sharks Belonging to the Squalus Genus (Squaliformes: Squalidae)
    Ailton A. Ariza, Aisni M. C. L. Adachi, Pollyana Roque, Fabio H. V. Hazin, Marcelo Vianna, Matheus M. Rotundo, Sergio M. Delpiani, Juan M. Díaz de Astarloa, Gabriela Delpiani, Claudio Oliveira, Fausto Foresti, Vanessa P. Cruz
    Diversity, 2022
    The Squalus genus comprises a group of small demersal sharks occurring circumglobally, popularly known as dogfish sharks. This genus exhibits a conserved morphology, thus making correct morphological identification difficult. Considering these taxonomic problems and the scarcity of molecular data, the present study aimed to identify Squalus genus MOTUs, using DNA barcoding for species delimitation via ABGD (automatic barcode gap discovery), PTP (Poisson tree process), and GMYC (general mixed Yule coalescent) employing the mitochondrial COI gene. A total of 69 sequences were generated from samples obtained from the American coast in both the Atlantic and Pacific Oceans. The ABGD analysis was the most conservative among the three applied delimitations, indicating three taxonomic units, while the PTP analysis revealed nine MOTUs, with two conflicting units noted between S. clarkae + S. mitsukurii and S. albicaudus + S. cubensis. The GMYC analysis indicated an excessive division, with S. acanthias and S. mitsukurii subdivided into six MOTUs each and S. blainville, into four. These findings demonstrated that Squalus presents a complex of previously defined species, with misidentified samples deposited in databases leading to difficulties in analyzing the real distribution and diversity of species belonging to this genus. Thus, further efforts to highlight possible new species are recommended.
  • Equatorial Atlantic pelagic predators reveal low content of PBDEs in contrast to MeO-BDEs: An analysis of brominated diphenyl ethers in blue shark and yellowfin tuna
    Dhoone Menezes-Sousa, Mariana Batha Alonso, Ana Carolina Pizzochero, Danielle Viana, Pollyana Roque, Fábio Hissa Vieira Hazin, João Paulo Machado Torres
    Science of the Total Environment, 2021
  • Morphological analysis and description of the ovaries of female silky sharks, carcharhinus falciformis (Müller & Henle, 1839)
    Mariana G. Rêgo, Fábio H. V. Hazin, Joaquim Evêncio Neto, P. G. V. Oliveira, Maria Goretti Soares, Keilla Regina L. S. Torres, Fernanda O. Lana, Pollyana C. G. Roque, Natália L. Santos, Rui Coelho
    Neotropical Ichthyology, 2013
    This work aims to study the female reproductive tract of silky sharks, Carcharhinus falciformis, captured in the South and Equatorial Atlantic Ocean. Samples were collected between January 2008 and March 2010 through oceanic commercial vessels that targeted tuna and swordfish, with a total of 17 females collected. The methodologies followed for analyzing the ovaries of those females included both macroscopic and histological analysis. Macroscopically, it was possible to determine that the ovaries on these sharks is suspended by mesenteries in the anterior section of the body cavity, heavily irrigated by blood vessels, and contains a wide range of oocytes. Ovaries were found in three distinct maturational stages: Stage I (Immature), Stage II (Maturing) and Stage III (Mature). Immature ovaries were small, with widths ranging from 1.0 to 3.1 cm, and had a gelatinous or granulose internal structure; maturing ovaries were slightly larger, ranging in width between 5.2 and 6.0 cm; mature ovaries ranged in width between 6.5 and 7.8 cm, and had a more rounded shape and the presence of large and well developed oocytes. Under microscopic examination, it was observed that the ovaries were covered with simple epithelial tissue during the early development stages and a simple cubic epithelium in the final stages of maturation. During the initial maturation stages the epigonal organ was not differentiated from the ovary. In mature specimens, the ovary showed a simple cubic epithelium and just below this epithelium there was a layer of dense connective tissue and muscle with the presence of vitellogenic oocytes and fat cells. A thin yolk membrane enclosing the oocytes was also evident. Finally, it was possible to distinguish a zona pellucida, separating the oocytes from the follicle wall and a basal lamina between the granular layers and the teak layer.
  • Reproductive aspects of the oceanic whitetip shark, Carcharhinus longimanus (Elasmobranchii: Carcharhinidae), in the equatorial and southwestern Atlantic Ocean
    Mirna Regina dos Santos Tambourgi, Fábio H.V. Hazin, Paulo G.V. Oliveira, Rui Coelho, George Burgess, Pollyana C. G. Roque
    Brazilian Journal of Oceanography, 2013