Bruno Alexandre Das Neves Pereira

@laqv.requimte.pt

PhD student, Chemistry Department
Faculty NOVA sciences and technology

Bruno Alexandre Das Neves Pereira


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https://researchid.co/pealbno

RESEARCH, TEACHING, or OTHER INTERESTS

Chemical Engineering, Biotechnology, Industrial and Manufacturing Engineering, Microbiology
4

Scopus Publications

91

Scholar Citations

2

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • Recovery of C-phycocyanin from a natural deep eutectic solvent extractant
    Bruno A. Pereira, Luís Costa, João G. Crespo, Carla Brazinha
    Separation and Purification Technology, 2026
  • Fast centrifugal partition chromatography for C-Phycocyanin purification from Limnospira platensis using eutectic solvent-based biphasic systems
    Bruno A. Pereira, Alexandra Conde, João Vasco Valente, Mara G. Freire, Sónia P.M. Ventura
    Separation and Purification Technology, 2026
    The development of sustainable separation strategies for high-value bioproducts from microalgae is essential for advancing integrated biorefinery approaches. This study investigates the use of eutectic solvent (ES)-based biphasic systems for the selective separation of C-phycocyanin (C-PC), a water-soluble pigment-protein complex, from the crude extract of Limnospira platensis ( L. platensis ). Two ES combinations were selected based on phase stability and partitioning behavior: (i) a hydrophilic ES composed of glucose:glycerol:water (1:2:2.5) diluted with deionized water and paired with the hydrophobic ES decanoic acid:menthol (2:1), and (ii) the same hydrophilic ES diluted with phosphate buffer solution (14 wt% ) and combined with a hydrophobic ES comprising octanoic acid:decanoic acid:dodecanoic acid (3:2:1). These systems were evaluated using ultra-fast centrifugal partition chromatography (FCPC) under optimized conditions to enhance phase retention and resolution. The first system demonstrated effective separation with partition coefficients of 1.53 for C-PC and 0.50 for chlorophyll, achieving high purity at low pigment concentrations (15.9 mg C-PC ∙L −1 purity 5 times higher towards total proteins and chlorophylls). In contrast, the second system yielded more concentrated fractions (up to 615.9 mg C-PC ∙L −1 purity 5 and 0.87 times higher towards total proteins and chlorophylls, respectively) but exhibited low selectivity, with both pigments presenting similar partition behavior (K p ≈ 0.03). Overall, the results feature the potential of ES-based systems for pigment purification and highlight the trade-offs between resolution, purity, and concentration. • Valorization of L. platensis by recovering C-phycocyanin. • ES-based biphasic systems to separate C-PC from proteins and chlorophylls . • FCPC as a simple, rapid, and feasible technology to purify C-phycocyanin. • FCPC as a technology with a straightforward scale-up.
  • Sustainable processing of microalgae protein: Design of biphasic partitioning systems based on natural deep eutectic solvents for C-phycocyanin recovery from model aqueous solutions
    Bruno A. Pereira, Cristina T. Matos, Luís Costa, Luísa M. Ferreira, João G. Crespo, Carla Brazinha
    Separation and Purification Technology, 2025
  • An integrated process for mixed culture production of 3-hydroxyhexanoate-rich polyhydroxyalkanoates from fruit waste
    Fernando Silva, Mariana Matos, Bruno Pereira, Cláudia Ralo, Daniela Pequito, Nuno Marques, Gilda Carvalho, Maria A.M. Reis
    Chemical Engineering Journal, 2022

RECENT SCHOLAR PUBLICATIONS

  • Recovery of C-phycocyanin from a natural deep eutectic solvent extractant
    BA Pereira, L Costa, JG Crespo, C Brazinha
    Separation and Purification Technology, 137837 , 2026
    2026
  • Fast centrifugal partition chromatography for C-Phycocyanin purification from Limnospira platensis using eutectic solvent-based biphasic systems
    BA Pereira, A Conde, JV Valente, MG Freire, SPM Ventura
    Separation and Purification Technology 382, 135616 , 2025
    2025
    Citations: 1
  • Sustainable processing of microalgae protein: Design of biphasic partitioning systems based on natural deep eutectic solvents for C-phycocyanin recovery from model aqueous …
    BA Pereira, CT Matos, L Costa, LM Ferreira, JG Crespo, C Brazinha
    Separation and Purification Technology 353, 128510 , 2025
    2025
    Citations: 18
  • An integrated process for mixed culture production of 3-hydroxyhexanoate-rich polyhydroxyalkanoates from fruit waste
    F Silva, M Matos, B Pereira, C Ralo, D Pequito, N Marques, G Carvalho, ...
    Chemical Engineering Journal 427, 131908 , 2022
    2022
    Citations: 72
  • Effect of phosphate and sulphate loading rate on the selection of PHA accumulating phototrophic mixed cultures
    BA das Neves Pereira
    PQDT-Global , 2018
    2018

MOST CITED SCHOLAR PUBLICATIONS

  • An integrated process for mixed culture production of 3-hydroxyhexanoate-rich polyhydroxyalkanoates from fruit waste
    F Silva, M Matos, B Pereira, C Ralo, D Pequito, N Marques, G Carvalho, ...
    Chemical Engineering Journal 427, 131908 , 2022
    2022
    Citations: 72
  • Sustainable processing of microalgae protein: Design of biphasic partitioning systems based on natural deep eutectic solvents for C-phycocyanin recovery from model aqueous …
    BA Pereira, CT Matos, L Costa, LM Ferreira, JG Crespo, C Brazinha
    Separation and Purification Technology 353, 128510 , 2025
    2025
    Citations: 18
  • Fast centrifugal partition chromatography for C-Phycocyanin purification from Limnospira platensis using eutectic solvent-based biphasic systems
    BA Pereira, A Conde, JV Valente, MG Freire, SPM Ventura
    Separation and Purification Technology 382, 135616 , 2025
    2025
    Citations: 1
  • Recovery of C-phycocyanin from a natural deep eutectic solvent extractant
    BA Pereira, L Costa, JG Crespo, C Brazinha
    Separation and Purification Technology, 137837 , 2026
    2026
  • Effect of phosphate and sulphate loading rate on the selection of PHA accumulating phototrophic mixed cultures
    BA das Neves Pereira
    PQDT-Global , 2018
    2018

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)


10.1016/j.