In vitro and in vivo treatment with propranolol influences the number of early preantral follicles in mice† Stella Pollyanne Oliveira, Camila Stefane Ferreira, Emily Joy Zaniker-Gomez, Mariam Charles, Larissa Aline Freitas, Franciel Batista Felix, Luiza Aparecida Ansaloni Chagas Pereira, Whocely Victor de Castro, Vanessa Pinho, Francesca E Duncan, Paulo Henrique Almeida Campos-Junior Biology of Reproduction, 2026 Stimulating follicle growth could have utility for fertility preservation before follicular culture or as part of ovarian stimulation protocol therapies for women with a limited ovarian reserve. Propranolol is a clinically approved beta-blocker. Beyond its beta-adrenergic receptors, recent investigations have suggested an alternative role in activating Mammalian target of rapamycin (mTOR). Therefore, this study aimed to evaluate the efficacy of propranolol treatment on early follicle growth, both in vitro and in vivo. For in vitro studies, neonatal mouse ovaries were cultured for up to 3 days in the presence or absence of propranolol and then processed for histological analysis, quantitative PCR (qPCR), RNA in situ hybridization, and western blot (WB). For in vivo experiments, mice were treated with a low or a high dose of propranolol or no drug for 15 days. Then, analyses were performed, including body and ovarian weight measurements, histological analyses, WB, and qPCR. In vitro experiments demonstrated that treatment decreased primordial follicles and increased transitional, primary, and secondary follicles. Consistent with this, propranolol treatment resulted in the downregulation of Pten and an increased presence of Cpeb1, a novel biomarker of follicle activation, in primordial follicles. In our in vivo studies, propranolol promoted follicle activation, increased PI3K-p110 levels, and decreased Pten expression. In summary, propranolol increases the transition of primordial follicles to more advanced stages of development. These findings highlight propranolol as a potential drug to induce, in vivo and in vitro, early preantral follicle growth, potentially improving fertility preservation techniques and studies.
Chronic fat consumption affects metabolic aspects of murine physiology and influences ovarian follicle atresia Ayodeji Ojo Oteyola, Isadora Maria Sátiro de Oliveira, Jonathas Medeiros de Almeida, Lucas Carvalho Cardoso, Thais de Merici Domingues e Paula, Julia Meireles Nogueira, Jade Carceroni de Sousa Carvalho, Henrique Martins Nogueira, Brenda Lee Simas Porto, Ana Paula Lucas Mota, Paulo Henrique Almeida Campos-Junior, Erika Cristina Jorge, Fernanda Radicchi Campos Lobato de Almeida Reproductive Toxicology, 2025
The benefits of high-intensity physical exercise before and after Parkinson’s disease induction in rats S. V. Silva, G. M. S. Moreira, P. H. A. Campos-Junior, L. C. M. Damázio Brazilian Journal of Biology, 2024 High-intensity physical activity is a non-pharmacological intervention that has been tested as a treatment for Parkinson’s disease (PD). The objective of the study was to investigate the benefits of high-intensity physical exercise on the number of neurons and astrocytes in a a rat model of Parkinson’s disease submitted to training before and after the inducing injury. Seventy Wistar rats were used, distributed as follows: nine rats trained before PD induction (DP-Exa), nine trained after PD induction (DP-Exd), 10 trained before and after PD induction (DP-Exad), and nine sedentary rats (DP-Sed). There were also the same groups but with the rats exposed to the sham surgery (control). High-intensity physical exercise on a vertical ladder was performed before and/or after PD induction for 5 days/week, 30-45 min a day, for 4 weeks. PD was induced with an electrolytic lesion (AP -4.9, ML 1.7, and DV 8.1). At the end of the experiment, the brain was removed for Nissl staining and immunohistochemistry of glial fibrillary acidid protein (GFAP) in the substantia nigra and striatum. The DP-Exa, Sham-Exa, DP-Exad, and Sham-Exad groups showed a greater number of neurons and higher expression of GFAP in the substantia nigra and stiatum compared with the the DP-Exd, Sham-Exd, DP-Sed, and Sham-Sed groups. Thus, rats that performed high-intensity training before or before and after PD induction had higher densities of neurons and astrocytes.
The Effects of Resistance Exercise on the Cardiorespiratory Tissue of Rats with Parkinson’s Disease Graziele Mayra Santos Moreira, Laila Cristina Moreira Damázio, Silvana Venâncio da Silva, Augusto Targino Silveira, Isabella Giordano Mesquita, Luana Aparecida de Sousa Silva, Luan Alves Pereira, Ana Clara Silva Costa, Ismael Augusto Lima Santos, Maria Eduarda Paiva Campos, Luna Sara Campos Vaz, Zenaide Alves Cardoso, José Victor Ribeiro Silva Gomes, Paulo Henrique Almeida Campos Júnior, Liliam Midori Ide International Journal of Environmental Research and Public Health, 2023 Parkinson’s Disease (PD) affects the cardiorespiratory system, causing an increase in the left ventricular mass in the heart and respiratory muscle weakness in relation to healthy individuals. The objective of this study was to investigate the effects of progressive resistive physical exercise on the vertical ladder on the histomorphometry of cardiac tissue and respiratory muscles in rats with PD. Seventy male Wistar rats, aged 40 days old, were divided into Parkinson’s (PD) and Sham (SH) groups; they were also subdivided into groups that performed progressive resistive physical exercise on the vertical ladder Before Surgery (ExBS), After Surgery (ExAS), and Before and After Surgery (ExBAS). The physical training was carried out before and/or after PD induction. Exercise was performed five times a week for 25 min/day for four or eight weeks. PD induction was conducted via electrolytic stimulation of the Substantia nigra of the animals’ brains, adopting the following stereotaxic table coordinates: −4.9; lateral medium equal to 1.7, and dorsoventral equal to 8.1. For the morphometric analysis of the heart, the following variables were calculated: relative weight and diameter and thickness of the left ventricle. The diaphragm and the myocardial, intercostal, and abdominal muscles were stained with Hematoxylin and Eosin (HE). The histomorphometric analysis was performed using the ImageJ software to assess the cross-sectional area of muscles and the number of muscle fibers. Progressive resistance exercise promoted the hypertrophy of respiratory muscles and the left ventricle in animals with PD.
Survey of third-party parenting options associated with fertility preservation available to patients with cancer around the globe Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C.M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Cláudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sánchez, Guillermo Terrado, Jhenifer K. Rodrigues, Joao Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Lígia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, María del Mar Andrés, Maria T. Bourlon, Mario Vega, Maurício Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff Journal of Global Oncology, 2020
Survey of fertility preservation options available to patients with cancer around the globe Alexandra S. Rashedi, Saskia F. de Roo, Lauren M. Ataman, Maxwell E. Edmonds, Adelino Amaral Silva, Anibal Scarella, Anna Horbaczewska, Antoinette Anazodo, Ayse Arvas, Bruno Ramalho de Carvalho, Cassio Sartorio, Catharina C.M. Beerendonk, Cesar Diaz-Garcia, Chang Suk Suh, Cláudia Melo, Claus Yding Andersen, Eduardo Motta, Ellen M. Greenblatt, Ellen Van Moer, Elnaz Zand, Fernando M. Reis, Flor Sánchez, Guillermo Terrado, Jhenifer K. Rodrigues, João Marcos de Meneses e Silva, Johan Smitz, Jose Medrano, Jung Ryeol Lee, Katharina Winkler-Crepaz, Kristin Smith, Lígia Helena Ferreira Melo e Silva, Ludwig Wildt, Mahmoud Salama, María del Mar Andrés, Maria T. Bourlon, Mario Vega, Maurício Barbour Chehin, Michel De Vos, Mohamed Khrouf, Nao Suzuki, Osama Azmy, Paula Fontoura, Paulo Henrique Almeida Campos-Junior, Peter Mallmann, Ricardo Azambuja, Ricardo M. Marinho, Richard A. Anderson, Robert Jach, Roberto de A. Antunes, Rod Mitchell, Rouhollah Fathi, Satish Kumar Adiga, Seido Takae, Seok Hyun Kim, Sergio Romero, Silvana Chedid Grieco, Talya Shaulov, Tatsuro Furui, Teresa Almeida-Santos, Willianne Nelen, Yasmin Jayasinghe, Yodo Sugishita, Teresa K. Woodruff Journal of Global Oncology, 2020
Spermatogonial Stem Cell Markers and Niche in Equids Guilherme M. J. Costa, Gleide F. Avelar, José V. Rezende-Neto, Paulo Henrique A. Campos-Junior, Samyra M. S. N. Lacerda, Bruno S. C. Andrade, Ralph Gruppi Thomé, Marie-Claude Hofmann, Luiz R. Franca Plos One, 2012
