Olexander Bevza

@biochemistry.org.ua

Biochemistry of muscles
Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Olexander Bevza


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https://researchid.co/ovbevza
Bevza O.V. in 1992 graduated from the Taras Shevchenko National University of Kyiv (Faculty of Biology, Department of Biochemistry) with a degree in biologist - biochemist, teacher of biology and chemistry. Since 1987, he has been working at the O.V. Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine in the following positions: laboratory assistant (1987-1990), engineer (1990-1997), acting junior research fellow (1997-1998), research fellow (since 1998), senior research fellow (since 2021). He defended his PhD thesis in 1998. Bevza O.V. is a specialist in the field of biochemistry. He is actively involved in the methods of preparative biochemistry, chemical and biochemical kinetics, biochemical membrane science, spectrophotometry, spectrofluorimetry, laser correlation spectroscopy, enzymology, and statistical analysis. He pays great attention to the implementation of modern computer modeling technologies. He is the author and co-author of more than 40 pub

EDUCATION

Taras Shevchenko Kyiv State University

RESEARCH, TEACHING, or OTHER INTERESTS

Biochemistry, Computer Science Applications
14

Scopus Publications

27

Scholar Citations

3

Scholar h-index

Scopus Publications

  • Thiacalix[4]arene С-1193 – a promising inhibitor of the sodium pump in the uterine smooth muscle cells
    , O. V. Maliuk, T. O. Veklich, , O. V. Tsymbalyuk, , O. V. Bevza, , S. O. Cherenok, , A. I. Selikhova, , V. I. Kalchenko, , S. O. Kosterin, and
    Ukrainian Biochemical Journal, 2025
    Thiacalix[4]arene C-1193 (25,27-dibutoxythiacalix[4]arene-bis-hydroxymethylphosphonic acid) was shown to inhibit the activity of Na+,K+-ATPase with a high efficiency (І0.5 = 42.1 ± 0.6 nM) with no effect on the activity of Mg2+-ATPase, Са2+-ATPase and Са2+,Mg2+-ATPase in the plasma membrane fraction of rat uterine smooth muscle cells. The kinetic regularities of the C-1193 inhibitory effect on Na+,K+-ATPase activity were investigated. It was demonstrated that C-1193 increased the enzyme activation constant by Na+ but not by K+ ions. The contractile activity of the rat uterine horns was investigated by tenzometric methods with the use of longitudinal uterine smooth muscle strips with intact endometrium. С-1193 induced a considerable increase in the amplitude of the acetylcholine-induced contractions as well as the maximal velocity of the contraction and relaxation phases. No effect of С-1193 on contractive activity induced by the selective agonist of М3-cholinoreceptors cevimeline was observed. The results of computer simulation showed that С-1193inhibitory effect must be related to the cooperative action of methylene bisphosphonate fragments on the upper rim of the calixarene platform, and the linker sulfur atoms of calixarene “cup” on the Na+,K+-ATPase macrostructure. Keywords: computer simulation, docking, K+-АТРase, myometrium, Na+, plasma membrane, smooth muscle cell, thiacalix[4]arenes
  • Тhiacalix[4]arene phosphonate C-800 as a novel fluorescent probe for zinc in living cells
    , V. I. Yavorovska, R. D. Labyntseva, , O. V. Bevza, , A. Y. Pugach, , A. B. Drapailo, , S. O. Cherenok, , V. I. Kalchenko, , S. O. Kosterin Kalchenko, and
    Ukrainian Biochemical Journal, 2021
    Zn ions are significant for maintaining the proper human organism functioning, thus monitoring the zinc content in living cells and the development of sensitive tracking systems and sensors for Zn is particularly important. The purpose of the work was to study the properties of synthetic thiacalix Our studies demonstrated that thiacalix Fluorescence intensity of thiacalix Computer modeling demonstrated that two Zn cations interact with the oxygen atoms of four hydroxy-ethoxyphosphonylmethyl groups. It was shown that thiacalix The addition of Zn 2+ to cells, stained with thiacalix[4]arene C-800, was followed an even greater increase of intracellular fluorescent signal intensity. No effect of thiacalix Thus, thiacalix[4]arene C-800 can potentially be used as a selective fluorescent probe for the detection of Zn 2+ in living cells.
  • CALIX[4]ARENES AS THE EFFECTORS OF SMOOTH MUSCLE MYOSIN ATPASE
    Encyclopedia of Cell Biology New Research 9 Volume Set, 2020
  • Calix[4]arenes as the effectors of smooth muscle myosin ATPase
    Myosin Biosynthesis Classes and Function, 2018
  • Thiacalix[4]arenes Remove the Inhibitory Effects of Zn Cations on the Myosin ATPase Activity
    Raisa Labyntsevа, Viktoriia Yavorovska, Olexander Bevza, Andriy Drapaylo, Vitaly Kalchenko, Sergiy Kosterin
    Nanoscale Research Letters, 2018
    Numerous female reproductive abnormalities are caused by uterine smooth muscle (myometrium) disorders. Heavy metals have an adverse effect on the contractility of the uterine smooth muscle. Although zinc is an essential biogenic element for most of the organisms, high doses of this element are toxic. The study of 0.5−5 mM Zn2+ effect on myosin S1 ATPase activity from the uterus found that 5 mM Zn2+ cations have the most pronounced inhibitory effect. The calculation of the kinetic parameters (Km and Vmax, ATP) revealed that the apparent maximum velocity of the hydrolysis ATP catalyzed by myosin in the presence of 5 mM Zn2+ decreased by 1.6 times. The value of Кm for ATP hydrolysis by myosin S1 in the presence of Zn2+ does not change statistically, although it tends to decrease. It was determined that uterine myosin S1 ATPase activity does not depend on the concentration of Mg2+ in the presence of 5 mM Zn2+. Also, it was demonstrated that tetrahydroxythiacalix[4]arene-tetrasulfosphonate (C-798) and tetrahydroxythiacalix[4]arene-tetraphosphonate (C-800) restored myosin S1 ATPase activity to the control level in the presence of 5 mM Zn2+. One of the most probable mechanisms of restoring the action of these thiacalix[4]arenes protective effect is based on its ability to chelate heavy metal cations from the incubation medium. The molecular docking of C-798 and C-800 into the myosin S1 region showed that these thiacalix[4]arenes could interact with Zn cation bond by myosin amino acid residues near the ATPase active site. Therefore, thiacalix[4]arenes may weaken the interaction between this cation and myosin S1. It was speculated that the obtained results could be used for further research with the aim of using this thiacalix[4]arenes as pharmacological compounds in the case of poisoning with high concentrations of zinc.
  • Calix[4]arene C-90 and its analogs activate at pase of the myometrium myosin subfragment-1
    , R. D. Labyntseva, O. V. Bevza, , K. V. Lytvyn, , M. O. Borovyk, , R. V. Rodik, , V. I. Kalchenko, , S. O. Kosterin, and
    Ukrainian Biochemical Journal, 2016
    Numerous female reproductive abnormalities are consequences of disorders in uterus smooth muscle (myometrium) contractile function. In this work, we described activators of ATPase, which could be used for development of effective treatments for correcting this dysfunction. Myosin ATPase localized in the catalytic domain of myosin subfragment-1 transforms a chemical energy deposited in macroergic bonds of ATP into mechanical movement. It was shown that сalix[4]arene C-90 and its structural analogs functionalized at the upper rim of macrocycle with four or at least two N-phenylsulfonуltrifluoroacetamidine groups, are able to activate ATP hydrolysis catalyzed by myometrium myosin subfragment-1. It was shown with the method of computer modeling that N-phenylsulfonуltrifluoroacetamidine groups of calix[4]arene C-90 interact with responsible for binding, coordination and the hydrolysis of ATP amino acid residues of myosin subfragment-1. The results can be used for further research aimed at using calix[4]arene C-90 and its analogs as pharmacological compounds that can effectively normalize myometrium contractile hypofunction.
  • Protective effect of tiacalix[4]arene-tetrasulphonate on heavy metal inhibition of myometrium myosin subfragment-1 ATP-hydrolase activity
    and R. D. Labyntsevа
    Ukrainian Biochemical Journal, 2014
    Heavy metals have a negative effect on the contractility of uterine smooth muscles (myometrium), these effects can lead to various pathologies of a women reproductive system. To overcome these effects the methods for correcting the myometrium contractile activity are to be developed. Catalyzed by myosin ATPase ATP hydrolysis is the most important reaction in the molecular mechanism of myometrium contraction. We have found an inhibitory effect of 0.03-0.3 mM Ni2+, Pb2+ and Cd2+ on enzymatic hydrolysis of ATP by myosin subfragment-1 obtained from swine uterine smooth muscles. We have demonstrated that 100 μM thiacalix[4]arene-tetrasulphonate (C-798) recovered to the control level of ATPase activity of myosin subfragment-1 in the presence of heavy metal cations. One of the most probable mechanisms of C-798 corrective activity is based on its ability to chelate heavy metals, thus cations Pb, Cd and Ni can be removed from the incubation medium. Computer simulation has demonstrated that the protective effect of C-798 may also be the result of weakening the interaction of heavy metal ions with amino acid residues of the myosin molecule near the active site of ATP hydrolase. The obtained results can be used for further research aimed at assessing the prospects of thiacalix[4]arene-tetrasulfonate as pharmacological compounds.
  • [The calix[4]arene C-107 is highly effective supramolecular inhibitor of the Na+,K(+)-ATPase of plasma membranes].
    Ukrainski Biokhimicheski Zhurnal, 2013
  • Kinetic regularities and mechanisms of action of calix[4]arene C-99 on ATPase activity of myosin subfragment-1 of myometrium
    Ukrain Skyi Biokhimichnyi Zhurnal, 2010
  • Computer design of interaction of calix[4]arene C-99 with subfragment-1 of myometrium myosin
    Ukrain Skyi Biokhimichnyi Zhurnal, 2010
  • Ca2+/H+ exchange through a plasma membrane in the system of mechanisms of Ca2+ and H+ transport
    Ukrain Skyi Biokhimichnyi Zhurnal, 2003
  • Thermobiosensors: features of design and function and prospects for their use
    Ukrainskii Biokhimicheskii Zhurnal, 2002
  • Effect of H+ Extra- And Intracell Buffering Capacity on the Activity Ca2+/H+ Exchange in Lymphocytes' Plasma Membrane
    Ukrain Skyi Biokhimichnyi Zhurnal, 1997
  • Effect of H+ extracellular and intracellular buffering capacity on the activity Ca2+/H+ exchange in the lymphocyte plasma membrane
    Ukrainskii Biokhimicheskii Zhurnal, 1997

RECENT SCHOLAR PUBLICATIONS

  • Тhiacalix [4] arene phosphonate C-800 as a novel fluorescent probe for zinc in living cells
    VI Yavorovska, RD Labyntseva, OV Bevza, AY Pugach, SO Kosterin, ...
    The Ukrainian Biochemical Journal 93 (4), 26-36 , 2021
    2021.0
    Citations: 2
  • Thiacalix [4] arenes Remove the Inhibitory Effects of Zn Cations on the Myosin ATPase Activity
    R Labyntsevа, V Yavorovska, O Bevza, A Drapaylo, V Kalchenko, ...
    Nanoscale Research Letters 13 (1), 224 , 2018
    2018.0
    Citations: 1
  • Calix| 4| arene C-90 and its analogs activate ATPase of the myometrium myosin subfragment-1
    RD Labyntseva, OV Bevza, KV Lytvyn, MO Borovyk, RV Rodik, ...
    The Ukrainian Biochemical Journal, 48-61 , 2016
    2016.0
    Citations: 4
  • [Protective effect of tiacalix [4] arene-tetrasulphonate on heavy metal inhibition of myometrium myosin subfragment-1 ATP-hydrolase activity].
    RD Labyntseva, OV Bevza, AA Bevza, AO Liul'ko, SH Kharchenko, ...
    Ukrainian Biochemical Journal 86 (6), 154-166 , 2014
    2014.0
    Citations: 2
  • [The calix [4] arene C-107 is highly effective supramolecular inhibitor of the Na+, K (+)-ATPase of plasma membranes].
    OV Bevza, TO Veklich, OA Shkrabak, RV Rodik, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 85 (2), 5-19 , 2013
    2013.0
    Citations: 2
  • [Structural and functional bases of the intermolecular interaction of calix [4] arene C-97 with myosin subfragment-1 of myometrium].
    RD Labyntseva, AA Bevza, OV Bevza, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 84 (1), 34-44 , 2012
    2012.0
    Citations: 2
  • [Kinetic regularities and mechanisms of action of calix [4] arene C-99 on ATPase activity of myosin subfragment-1 of myometrium].
    AA Bevza, RD Labyntseva, OV Bevza, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 82 (6), 22-32 , 2010
    2010.0
    Citations: 4
  • [Computer modeling of interaction of calix [4] arene C-99 with subfragment-1 of myometrium myosin].
    OV Bevza, AA Bevza, RD Labyntseva, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 82 (2), 85-93 , 2010
    2010.0
  • Computer design of interaction of calix [4] arene C-99 with subfragment-1 of myometrium myosin
    OV Bevza, AA Bevza, RD Labintseva, SO Kosterin, SO Cherenok, ...
    Украïнський бiохiмiчний журнал 82 (2), 85-93 , 2010
    2010.0
    Citations: 3
  • Ca2+/H+ exchange through plasma membrane in the system of transport of calcium and hydrogen ions
    OV Bevza
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 75 (5), 28-40 , 2003
    2003.0
  • Ca 2+ /H + exchange through a plasma membrane in the system of mechanisms of Ca 2+ and H + transport
    OV Bevza
    Украïнський бiохiмiчний журнал 75 (5), 28-40 , 2003
    2003.0
  • Thermobiosensors: features of design and function and prospects for their use
    OV Bevza, OM Shmyr'ova, MF Starodub
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 74 (2), 10-20 , 2002
    2002.0
    Citations: 1
  • Thermobiosensors: peculiarities of construction, functioning and perspective of practical application
    OV Bevza, AN Shmireva, NF Starodub
    Ukr. Biochem. J 74 (2), 10-20 , 2002
    2002.0
    Citations: 4
  • Thermobiosensors: constructive and functioning properties and practical their utilization
    OV Bevza, ON Shmyreva, NF Starodub
    UKRAINSKII BIOKHIMICHESKII ZHURNAL 74 (2), 10-20 , 2002
    2002.0
  • Effect of H + Extra- And Intracell Buffering Capacity on the Activity Ca 2+ /H + Exchange in Lymphocytes' Plasma Membrane
    OV Bevza, SM Kucherenko
    Украïнський бiохiмiчний журнал 69 (5-6), 91-91 , 1997
    1997.0
  • Effect of H+ extracellular and intracellular buffering capacity on the activity Ca2+/H+ exchange in the lymphocyte plasma membrane
    OV Bevza, SM Kucherenko
    Украïнський бiохiмiчний журнал 69 (5-6), 85-91 , 1997
    1997.0
    Citations: 1
  • Thiacalix [4] arene С-1193–a promising inhibiTor of The sodium pump in The uTerine smooTh muscle cells
    OV MalIUk, TO VeklIch, OV TSyMBalyUk, OV BeVza
    Citations: 1
  • EFFECT OF H+ EXTRA-AND INTRACELL BUFFERING CAPACITY ON THE ACTIVITY CA2+
    OV BEVZA, SM KUCHERENKO

MOST CITED SCHOLAR PUBLICATIONS

  • Calix| 4| arene C-90 and its analogs activate ATPase of the myometrium myosin subfragment-1
    RD Labyntseva, OV Bevza, KV Lytvyn, MO Borovyk, RV Rodik, ...
    The Ukrainian Biochemical Journal, 48-61 , 2016
    2016.0
    Citations: 4
  • [Kinetic regularities and mechanisms of action of calix [4] arene C-99 on ATPase activity of myosin subfragment-1 of myometrium].
    AA Bevza, RD Labyntseva, OV Bevza, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 82 (6), 22-32 , 2010
    2010.0
    Citations: 4
  • Thermobiosensors: peculiarities of construction, functioning and perspective of practical application
    OV Bevza, AN Shmireva, NF Starodub
    Ukr. Biochem. J 74 (2), 10-20 , 2002
    2002.0
    Citations: 4
  • Computer design of interaction of calix [4] arene C-99 with subfragment-1 of myometrium myosin
    OV Bevza, AA Bevza, RD Labintseva, SO Kosterin, SO Cherenok, ...
    Украïнський бiохiмiчний журнал 82 (2), 85-93 , 2010
    2010.0
    Citations: 3
  • Тhiacalix [4] arene phosphonate C-800 as a novel fluorescent probe for zinc in living cells
    VI Yavorovska, RD Labyntseva, OV Bevza, AY Pugach, SO Kosterin, ...
    The Ukrainian Biochemical Journal 93 (4), 26-36 , 2021
    2021.0
    Citations: 2
  • [Protective effect of tiacalix [4] arene-tetrasulphonate on heavy metal inhibition of myometrium myosin subfragment-1 ATP-hydrolase activity].
    RD Labyntseva, OV Bevza, AA Bevza, AO Liul'ko, SH Kharchenko, ...
    Ukrainian Biochemical Journal 86 (6), 154-166 , 2014
    2014.0
    Citations: 2
  • [The calix [4] arene C-107 is highly effective supramolecular inhibitor of the Na+, K (+)-ATPase of plasma membranes].
    OV Bevza, TO Veklich, OA Shkrabak, RV Rodik, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 85 (2), 5-19 , 2013
    2013.0
    Citations: 2
  • [Structural and functional bases of the intermolecular interaction of calix [4] arene C-97 with myosin subfragment-1 of myometrium].
    RD Labyntseva, AA Bevza, OV Bevza, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 84 (1), 34-44 , 2012
    2012.0
    Citations: 2
  • Thiacalix [4] arenes Remove the Inhibitory Effects of Zn Cations on the Myosin ATPase Activity
    R Labyntsevа, V Yavorovska, O Bevza, A Drapaylo, V Kalchenko, ...
    Nanoscale Research Letters 13 (1), 224 , 2018
    2018.0
    Citations: 1
  • Thermobiosensors: features of design and function and prospects for their use
    OV Bevza, OM Shmyr'ova, MF Starodub
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 74 (2), 10-20 , 2002
    2002.0
    Citations: 1
  • Effect of H+ extracellular and intracellular buffering capacity on the activity Ca2+/H+ exchange in the lymphocyte plasma membrane
    OV Bevza, SM Kucherenko
    Украïнський бiохiмiчний журнал 69 (5-6), 85-91 , 1997
    1997.0
    Citations: 1
  • Thiacalix [4] arene С-1193–a promising inhibiTor of The sodium pump in The uTerine smooTh muscle cells
    OV MalIUk, TO VeklIch, OV TSyMBalyUk, OV BeVza
    Citations: 1
  • [Computer modeling of interaction of calix [4] arene C-99 with subfragment-1 of myometrium myosin].
    OV Bevza, AA Bevza, RD Labyntseva, SO Cherenok, VI Kal'chenko, ...
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 82 (2), 85-93 , 2010
    2010.0
  • Ca2+/H+ exchange through plasma membrane in the system of transport of calcium and hydrogen ions
    OV Bevza
    Ukrains' kyi Biokhimichnyi Zhurnal (1999) 75 (5), 28-40 , 2003
    2003.0
  • Ca 2+ /H + exchange through a plasma membrane in the system of mechanisms of Ca 2+ and H + transport
    OV Bevza
    Украïнський бiохiмiчний журнал 75 (5), 28-40 , 2003
    2003.0
  • Thermobiosensors: constructive and functioning properties and practical their utilization
    OV Bevza, ON Shmyreva, NF Starodub
    UKRAINSKII BIOKHIMICHESKII ZHURNAL 74 (2), 10-20 , 2002
    2002.0
  • Effect of H + Extra- And Intracell Buffering Capacity on the Activity Ca 2+ /H + Exchange in Lymphocytes' Plasma Membrane
    OV Bevza, SM Kucherenko
    Украïнський бiохiмiчний журнал 69 (5-6), 91-91 , 1997
    1997.0
  • EFFECT OF H+ EXTRA-AND INTRACELL BUFFERING CAPACITY ON THE ACTIVITY CA2+
    OV BEVZA, SM KUCHERENKO