Ternopil state medical university, Ukraine
Pathophysiology Professional Doctoral degree/MD, 2011
L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine, Infection diseases Research Doctoral degree/PhD, 2006
Vinnytsya State Progov Medical University, Ukraine, Pediatrics, Master, 2001
National Pirogov Memorial Medical University, Vinnytsya, Ukraine, Doctor of Medicine, 2000
RESEARCH INTERESTS
Pathophysiology
Gut microbiome
Liver pathology
Brain-gut axis
6
Scopus Publications
297
Scholar Citations
9
Scholar h-index
6
Scholar i10-index
Scopus Publications
The gut microbiome and the brain Nadiia Rykalo, Lydia Riehl, Michaela Kress Current Opinion in Supportive and Palliative Care, 2024 Purpose of review The importance of the gut microbiome for human health and well-being is generally accepted, and elucidating the signaling pathways between the gut microbiome and the host offers novel mechanistic insight into the (patho)physiology and multifaceted aspects of healthy aging and human brain functions. Recent findings The gut microbiome is tightly linked with the nervous system, and gut microbiota are increasingly emerging as important regulators of emotional and cognitive performance. They send and receive signals for the bidirectional communication between gut and brain via immunological, neuroanatomical, and humoral pathways. The composition of the gut microbiota and the spectrum of metabolites and neurotransmitters that they release changes with increasing age, nutrition, hypoxia, and other pathological conditions. Changes in gut microbiota (dysbiosis) are associated with critical illnesses such as cancer, cardiovascular, and chronic kidney disease but also neurological, mental, and pain disorders, as well as chemotherapies and antibiotics affecting brain development and function. Summary Dysbiosis and a concomitant imbalance of mediators are increasingly emerging both as causes and consequences of diseases affecting the brain. Understanding the microbiota’s role in the pathogenesis of these disorders will have major clinical implications and offer new opportunities for therapeutic interventions.
Investigation of nuclear DNA content and cell cycle phases in rat liver cells under chlorpromazine administration N. A. Rykalo, O. V. Baylo Reports of Morphology, 2023 Hepatotoxicity of antipsychotic drugs remains an urgent problem of modern medicine. Therefore, the purpose of the study was to investigate the nuclear DNA content and cell cycle phases of rat liver cells under Сhlorpromazine administration at doses ranging from 3.5 mg/kg to 28 mg/kg for 30 and 60 days. The study was conducted on 60 sexually mature female rats. Chlorpromazine was administered once daily for 30 and 60 days at doses of 3.5 mg/kg, 7 mg/kg, 14 mg/kg, 21 mg/kg and 28 mg/kg. The DNA content in the nuclei of rat liver cells was determined by flow cytometry. Cytological analysis of cells was performed using FloMax software (Partec, Germany), where the percentage of nuclei in the G0G1 interval of the cell cycle, in the S phase, G2M interval, and the apoptosis index – SUB-G0G1 area on DNA histograms were determined. Statistical processing of the results was performed using the Mann-Whitney U test. The results of the study showed that Сhlorpromazine has a dose-dependent hepatotoxic effect: with an increase in the dose of this drug in rats from 7 to 28 mg/kg, the percentage of fragmented nuclei in liver tissue significantly increased, which is a sign of hepatocyte death by apoptosis. It was found that Сhlorpromazine at a dose of 3.5 mg/kg did not increase hepatocyte apoptosis, while at a dose of 21 and 28 mg/kg the drug showed the highest hepatotoxicity, increasing the level of apoptosis by 1.9 and 2.1 (p˂0.05) times, respectively. The hepatotoxic effect is enhanced by the use of Сhlorpromazine for 60 days, which is manifested in a significant increase in hepatocyte nuclear DNA fragmentation, which, in our opinion, should be taken into account when conducting long-term therapy in patients.
Morphological changes in the liver of rats after administration of chlorpromazine, depending on the dose and duration of administration O. V. Bailo, N. A. Rykalo Reports of Morphology, 2023 Chlorpromazine (CPZ) remains a widely used drug in psychiatric practice today. The drug has a hepatotoxic effect, but the possible mechanisms of this side effect have not yet been fully elucidated. The aim of the study was to determine morphological changes in rat liver tissue under chronic toxic effects of chlorpromazine, depending on the dose and duration of its administration. The study was conducted on 60 sexually mature male rats. CPP was administered intragastrically at different doses (3.5, 7.0, 14.0 and 21.0 mg/kg) for 30 and 60 days. The material was fixed in a 10 % solution of neutral formalin (pH 7.2-7.4) for 24-48 hours, then passed through alcohols of increasing concentration and embedded in paraffin. Serial sections (6-7 µm thick) were prepared from the paraffin blocks and stained with hematoxylin-eosin and picrofuchsin by Van Gieson to determine the degree of fibrotic changes in liver tissue, as well as with Giemsa III to detect fatty degeneration of hepatocytes. The microscopic structure of the hepatic parenchyma was studied using an OLIMPUS BX41 light microscope at 100, 200 and 400x magnification. Morphometric parameters of structural changes were determined using an ocular grid and Image Tulsa 3.6 software. The data were statistically processed by descriptive statistics using the Microsoft Office Excel 2010 spreadsheet processor. When CPZ was administered in different doses and duration, pathological changes of varying severity developed in the liver tissue of rats. In the liver tissue, signs of intracellular and intra-tubular cholestasis are found mainly in the central lobes, accompanied by focal desquamation and proliferation of the biliary epithelium, formation of small-focal, less frequently zonal necrosis of hepatocytes, inflammatory infiltration of portal tracts with its spread to the interlobular stroma and parenchyma. Mitotically active binucleated hepatocytes are the key to the reparative process. Periductal fibrosis develops in the portal sections, marginal proliferation of the bile ducts, hepatocytes with signs of granular and/or fatty dystrophy are noted. In the central veins and vessels of the portal areas, moderate initial sclerotic changes were found, signs of their capillarisation in sinusoids, and the endothelium of the vessels had focal destructive changes. In all portal zones, proliferation of bile ducts and formation of bile pseudo-ducts were observed. Thus, the analysis of the morphometric study data showed that within 60 days of CPZ administration there is a significant increase in the relative volume of connective tissue and stromal-parenchymal index due to a significant decrease in the volume of hepatocytes.
The importance of the gut microbiome and its signals for a healthy nervous system and the multifaceted mechanisms of neuropsychiatric disorders Lydia Riehl, Johannes Fürst, Michaela Kress, Nadiia Rykalo Frontiers in Neuroscience, 2023 Increasing evidence links the gut microbiome and the nervous system in health and disease. This narrative review discusses current views on the interaction between the gut microbiota, the intestinal epithelium, and the brain, and provides an overview of the communication routes and signals of the bidirectional interactions between gut microbiota and the brain, including circulatory, immunological, neuroanatomical, and neuroendocrine pathways. Similarities and differences in healthy gut microbiota in humans and mice exist that are relevant for the translational gap between non-human model systems and patients. There is an increasing spectrum of metabolites and neurotransmitters that are released and/or modulated by the gut microbiota in both homeostatic and pathological conditions. Dysbiotic disruptions occur as consequences of critical illnesses such as cancer, cardiovascular and chronic kidney disease but also neurological, mental, and pain disorders, as well as ischemic and traumatic brain injury. Changes in the gut microbiota (dysbiosis) and a concomitant imbalance in the release of mediators may be cause or consequence of diseases of the central nervous system and are increasingly emerging as critical links to the disruption of healthy physiological function, alterations in nutrition intake, exposure to hypoxic conditions and others, observed in brain disorders. Despite the generally accepted importance of the gut microbiome, the bidirectional communication routes between brain and gut are not fully understood. Elucidating these routes and signaling pathways in more detail offers novel mechanistic insight into the pathophysiology and multifaceted aspects of brain disorders.
Possibilities for implementing of anti-fibrotic and anti-inflammatory effects of metabolic therapy in acute alcoholic disorders under experimental conditions N. A. Rykalo, I. V. Romanenko Zaporozhye Medical Journal, 2022 The aim of the work was to study antifibrotic and anti-inflammatory effects of metabolic therapy and mechanisms of regeneration in acute alcoholic liver damage (AALD) in rats under experimental conditions. Materials and methods. The experiment involved 66 white non-linear male rats with a mass of 120–130 g, which were divided into 5 groups: 1 – intact animals (n = 10); 2 – animals with AALD (n = 20), 3 – animals (n = 12) with AALD and intraperitoneally injected with Corvitin, 4 – animals (n = 12) with AALD and injected with Glutargin, 5 – аnimals with AALD (n = 12) and injected with Corvitin and Glutargin. The pro-inflammatory cytokines and cell cycle phases were analyzed. Results. The level of IGF-1 was significantly 24.1 % higher in group 2 compared to the control. In animals of group 3, the level of IGF-1 was 20.2 % decreased compared with group 2. The level of IGF-1 was significantly 9.7 % decreased in group 5 animals compared with group 2. There was a 31.6% increase in the level of TGF-β in animals of group 2 in comparison with the control ones. The level of TGF-β was 22.8 % decreased in group 3 compared with group 2. In group 5 animals, the value was 12.0 % lower than in group 2. The percentage of cell nuclei in the presynthetic phase in group 2 rats was 8.3 % higher than in controls. In animals of group 2, the number of cell nuclei in the phase of DNA synthesis were 33.3 % larger than in group 1. The rate of DNA fragmentation in AALD exceeded the corresponding value in control group by 27.5 %. Conclusions. AALD was accompanied by an increase in the serum IGF-1 and TGF-β in animals. The administration of corvitin decreased the level of IGF-1 in rats with AALD, and the use of glutargine mainly decreased the level of TGF-β. Combined use of the drugs did not show significant effectiveness. Compensatory regeneration mechanisms were activated in AALD processes and apoptotic cell death was evidenced by the increased indicators of nuclear DNA fragmentation.
The gut microbiome and the brain N Rykalo, L Riehl, M Kress Current Opinion in Supportive and Palliative Care 18 (4), 282-291 , 2024 2024 Citations: 11
The importance of the gut microbiome and its signals for a healthy nervous system and the multifaceted mechanisms of neuropsychiatric disorders L Riehl, J Fürst, M Kress, N Rykalo Frontiers in Neuroscience 17, 1-20 , 2024 2024 Citations: 31
Investigation of nuclear DNA content and cell cycle phases in rat liver cells under chlorpromazine administration NA Rykalo, OV Baylo Reports of Morphology 29 (3), 26-31 , 2023 2023
Morphological changes in the liver of rats after administration of chlorpromazine, depending on the dose and duration of administration O Bailo, N Rykalo Reports of Morphology 29 (1), 67-76 , 2023 2023 Citations: 4
Вплив лізиноприлу та L-аргініну L-глутамату на біохімічні маркери фіброзу в організмі щурів за хронічного токсичного гепатиту НА Рикало, ЮМ Олійник Фармакологія та лікарська токсикологія 16 (3), 187-194 , 2022 2022 Citations: 1
Можливості реалізації антифібротичних і протизапальних ефектів метаболічної терапії гострого алкогольного ураження печінки в умовах експерименту NA Rykalo, IV Romanenko Zaporozhye Medical Journal 24 (4), 396-401 , 2022 2022 Citations: 1
Медикаментозна корекція синдромів цитолізу та холестазу при гострому алкогольному гепатиті в експерименті НА Рикало, ІВ Романенко Актуальні проблеми транспортної медицини , 2022 2022 Citations: 1
Possibilities for implementing of anti-fibrotic and anti-inflammatory effects of metabolic therapy in acute alcoholic disorders under experimental conditions N Rykalo, I Romanenko Zaporozhye medical journal 24 (4), 396-401 , 2022 2022 Citations: 2
НАПРЯМКИ ПІДГОТОВКИ СТУДЕНТІВ-МЕДИКІВ ДО СКЛАДАННЯ ЛІЦЕНЗІЙНОГО ІСПИТУ «КРОК-1». НА Рикало, ВВ Піліпонова EDITORIAL BOARD, 552 , 2021 2021
ВИКОРИСТАННЯ МЕТОДИК СТРУКТУРНО-ЛОГІЧНИХ ТЕХНОЛОГІЙ ПРИ ВИКЛАДАННІ СТУДЕНТАМ ЗАОЧНОЇ ФОРМИ НАВЧАННЯ У МЕДИЧНИХ ВУЗАХ НА Рикало, АО Іваниця The 4 th International scientific and practical conference―Priority … , 2020 2020
STUDY OF HEPATOTOXICITY OF DIFFERENT DOSES OF CHLORPROMAZINE IN A CHRONIC EXPERIMENT IN RATS RN Anatoliivna, BO Viktorivna The 9th International scientific and practical conference “Modern science … , 2020 2020
ПЕПТИДНО-ЗВ'ЯЗАНИЙ ГІДРОКСИПРОЛІН ЯК МАРКЕР ФІБРОТИЧНИХ ЗМІН ТКАНИНИ ПЕЧІНКИ НА Рикало, ЮМ Береговенко ВІД ЕКСПЕРИМЕНТАЛЬНОЇ ТА КЛІНІЧНОЇ ПАТОФІЗІОЛОГІЇ ДО ДОСЯГНЕНЬ СУЧАСНОЇ … , 2020 2020
Сoncentration of IGF-1 and TGF-β in the blood plasma of rats with chronic toxic hepatitis and its correction with lisinopril NA Rykalo, SA Semenchuk, AO Ivanytsia, OY Mikhailivna Азербайджанский медицинский журнал, 89-95 , 2020 2020 Citations: 2
Morphological changes in kidney glomeruli of rats with chronic toxic hepatitis and its correction with lisinopril, L-arginine-L-glutamate and their combination NA Rykalo, YM Berehovenko, OV Androshchuk Journal of Education, Health and Sport 9 (5), 632-639 , 2019 2019 Citations: 3
Шляхи оптимізації викладання предмету" Патофізіологія" та підготовка майбутніх лікарів на базі Вінницького національного медичного університету ім. МІ Пирогова НА Рикало, ВВ Піліпонова Вісник ВНМУ , 2019 2019
СТРУКТУРНІ ЗМІНИ ПЕЧІНКИ ПРИ ГОСТРОМУ АЛКОГОЛЬНОМУ ГЕПАТИТІ ТА ЗА УМОВ МЕДИКАМЕНТОЗНОЇ КОРЕКЦІЇ НА Рикало, ІВ Романенко БЮЛЛЕТЕНЬ XVII ЧТЕНИЙ ИМ. ВВ ПОДВЫСОЦКОГО, 146 , 2018 2018 Citations: 1
Morphological changes in kidney tissues of rats with acute ethanol-induced injury and after drug correction NA Rikalo, IV Romanenko Journal of Education, Health and Sport 8 (2), 272-279 , 2018 2018 Citations: 3
СТРУКТУРНІ ЗМІНИ КАНАЛЬЦЕВОГО КОМПОНЕНТУ НИРОК ЩУРІВ ПРИ ХРОНІЧНОМУ ТОКСИЧНОМУ ГЕПАТИТІ ТА ЙОГО МЕДИКАМЕНТОЗНІЙ КОРЕКЦІЇ НА РИКАЛО, ЮМ БЕРЕГОВЕНКО BBK 72 D730, 83 , 2018 2018
Вплив протизапального цитокіна ІGF-1 на репаративно-регенеративні процеси печінки у щурів різного віку на тлі хронічної алкогольної інтоксикації та корекції. НА Рикало, ЛО Яровенко Biobank Association of Ukraine , 2018 2018
КЛЮЧОВІ АСПЕКТИ ВИКЛАДАННЯ ПАТОЛОГІЧНОЇ ФІЗІОЛОГІЇ СТУДЕНТАМ СТОМАТОЛОГІЧНОГО ФАКУЛЬТЕТУ Н Рикало, О Андрощук Modern Information Technologies and Innovation Methodologies of Education in … , 2018 2018
MOST CITED SCHOLAR PUBLICATIONS
The importance of the gut microbiome and its signals for a healthy nervous system and the multifaceted mechanisms of neuropsychiatric disorders L Riehl, J Fürst, M Kress, N Rykalo Frontiers in Neuroscience 17, 1-20 , 2024 2024 Citations: 31
Синдром недиференційованої дисплазії сполучної тканини: від крнцепції патогенезу до стратегії лікування.: Навчальний посібник для ВМНЗ ІІІ-ІV р. а. ОВ Солєйко, НА Рикало Нова Книга , 2014 2014 Citations: 18
Експериментальна модель хронічного тетрахлорметанового гепатиту та цирозу печінки у нестатевозрілих щурів НА Рикало Актуальні проблеми сучасної медицини: Вісник української медичної … , 2009 2009 Citations: 14
The gut microbiome and the brain N Rykalo, L Riehl, M Kress Current Opinion in Supportive and Palliative Care 18 (4), 282-291 , 2024 2024 Citations: 11
Експериментальна модель хронічного медикаментозного гепатиту у статевонезрілих щурів НА Рикало, ОЮ Гумінська, ОВ Андрощук Таврический медико-биологический вестник 15 (3-1), 283-286 , 2012 2012 Citations: 11
Syndrome of undifferentiated connective tissue dysplasia: from the concept of pathogenesis to treatment strategy OV Solyeyko, NA Rykalo, IP Osypenko, LP Soleyko Tutorial. Vinnytsia, Ukraine: Nova Knyha , 2014 2014 Citations: 10
Патогенез хронічних вірусних гепатитів В і С у дітей: вікові особливості, патогенетична терапія (експериментально-клінічне дослідження) НА РИКАЛО Вінниця , 2011 2011 Citations: 9
Experimental model of chronic tetrachloromethane hepatitis and liver cirrhosis in immature rats NA Rykalo Actual problems of modern medicine: Bulletin of the Ukrainian Medical … , 2009 2009 Citations: 9
Сучасні погляди на патогенез гострого алкогольного гепатиту і можливості його лікування НА Рикало, ІВ Романенко Вісник Вінницького національного медичного університету, 641-645 , 2014 2014 Citations: 8
Особливості перебігу та лікування сальмонельозу та клебсієльозу у дітей в залежності від характеру мікрофлори біотопів НА Рикало Київ, 2006.–20 с , 2006 2006 Citations: 8
Eksperymentalʹna modelʹ khronichnoho tetrakhlormetanovoho hepatytu ta tsyrozu pechinky u nestatevozrilykh shchuriv NA Rykalo Visnyk Ukrayinsʹkoyi medychnoyi stomatolohichnoyi akademiyi 9 (2), 116-118 , 2009 2009 Citations: 7
Взаємозв’язки між показниками кардюінтервалографії та антропо-соматотипологічними параметрами у здорових міських юнаків і дівчат Поділля ВВ Пилипонова, НА Рикало Актуальні проблеми сучасної медицини: Вісник української медичної … , 2011 2011 Citations: 6
Вплив гепатопротекторів на клітинні механізми репаративної регенерації тканини печінки при хронічному токсичному гепатиті у статевонезрілих щурів ВМ Мороз, НА Рикало Журнал АМН України 16 (4), 701-712 , 2010 2010 Citations: 6
ОСОБЛИВОСТІ МОРФОЛОГІЧНОЇ БУДОВИ СЕЛЕЗІНКИ СТАТЕВО-НЕЗРІЛИХ ЩУРІВ НА ТЛІ ХРОНІЧНОГО МЕДИКАМЕНТОЗНОГО ГЕПАТИТУ НА Рикало, ОЮ Гумінська, ОІ Тереховська Вісник наукових досліджень , 2013 2013 Citations: 5
Влияние инсулиноподобного фактора-1 на реперативную регенерацию печени при хроническом медикаментозном гепатите у неполовозрелых крыс НА Рыкало, ОВ Андрощук Современная медицина: актуальные вопросы, 150-160 , 2013 2013 Citations: 5
Morphological changes in the liver of rats after administration of chlorpromazine, depending on the dose and duration of administration O Bailo, N Rykalo Reports of Morphology 29 (1), 67-76 , 2023 2023 Citations: 4
Патоморфологічні зміни печінки та біохімічні зміни сироватки крові при гострому алкогольному гепатиті в умовах експерименту НА Рикало, ІВ Романенко Експериментальна і клінічна медицина 71 (2), 156-160 , 2016 2016 Citations: 4
Features of reparative regeneration of the liver tissue in rats during experimental tetrachloromethanic and alcoholic hepatitis NA Rikalo, LO Yarovenko Pathologia, 84-89 , 2015 2015 Citations: 4
Інсуліноподібний фактор росту-1 у патогенезі хронічного алкогольного ушкодження печінки щурів різних вікових груп НА Рикало, ЛО Яровенко Мир медицины и биологии 11 (4-1 (53)), 129-136 , 2015 2015 Citations: 4
