Study on MTHFR (C677T & A1298C) Gene Polymorphisms in the Condition of Glucose Intolerance During Pregnancy Bunga Papa Kusuma, D. Lakshmi Lalitha, Nagarjuna Sivaraj, Vijaya Sirisha Balaga, Arun Kumar Rao Panchanani, L V Simhachalam Kutikuppala, S. Sai Roshan, K. Kavya, Golla Varshitha American Journal of Reproductive Immunology, 2025 IntroductionGestational diabetes mellitus (GDM) is a prevalent medical complication in pregnancy, characterized by glucose intolerance. The global expected prevalence of GDM is approximately 15.1%. This study builds upon previous research by investigating elevated hematological parameters and exploring MTHFR gene polymorphisms in GDM.Materials and MethodsThis study included 304 pregnant women, comprising 152 patients with GDM and an equal number of normal pregnant women. Employing PCR‐RFLP techniques, we identified MTHFR gene polymorphisms (C677T & A1298C) associated with gestational diabetes.ResultsSignificant associations were found in gestational age and platelet count, indicating their relevance to GDM risk. The odds ratios for both MTHFR A1298C (p value: 0.034; OR = 0.7; 95% CI: 0.5021–0.9758) and MTHFR C677T (p value: 0.008; OR = 0.5453; 95% CI: 0.3292–0.9011) gene polymorphisms demonstrated an increased risk of GDM development.ConclusionThe study reveals that elevated platelet count and polymorphs increase the risk of developing GDM in pregnant women. Gestational age also plays a role. The study also finds a link between the MTHFR C677T SNP and the MTHFR A1298C gene polymorphism and the risk of gestational diabetes in Andhra Pradesh. This gives us new information about genetic and hematological factors.
Prevalence of resistant hypertension and its relation with plasma lipoprotein‑associated phospholipase A2 Sri Harsha Boppana, Nikhil Reddy Ravula, L. V. Simhachalam Kutikuppala, Nagarjuna Sivaraj, C. H. Raja Hamsa, Sidharth Mahajan, P. Siva Chaitanya International Journal of Noncommunicable Diseases, 2023 Background: A major modifiable risk factor for cardiovascular disease is hypertension. Resistant hypertension is observed to be associated with an increased likelihood of deleterious effects such as cardiovascular events, cerebrovascular accident, and renal dysfunction. The raised levels of inflammatory cytokines in the plasma like the C-reactive protein (CRP) have previously been observed to be significantly raised in hypertensive patients, but the overlying mechanisms corresponding to these processes remain unknown. Materials and Methods: In essence, this was an observational study with data collected from a population at a specific moment in the time to assess the relationships between the variables analyzed. A total of 200 individuals with arterial hypertension took part in the study. Fasting venous blood was obtained to evaluate plasma lipoprotein-related phospholipase A2 (Lp-PLA2) activity, and baseline data were collected. To diagnose RH, a 24-h ambulatory blood pressure management was done. Results: RH was observed among 100 patients and found to be predominant among males and older individuals, who were smokers and having hypertension for a longer period of time with higher Lp-PLA2 activity. More RH patients used calcium channel blockers and diuretics, while a fewer used angiotensin-converting enzyme inhibitors, angiotensin receptor blockers, and statins (P 0.05 for all comparisons). Conclusion: The plasma Lp-PLA2 activity beyond a certain threshold suggests a raised risk of RH, and the statins can help in reducing the RH incidence among persons with high Lp-PLA2 levels.
Association of TNFSF11 rs2200287 and TNFSF11 rs2148072 gene polymorphisms in preeclampsia Nagarjuna Sivaraj, Bunga Papa Kusuma, L V Simhachalam Kutikuppala, K Vijaya Rachel, Vijaya Sirisha Balaga, Samhitha Gundakaram American Journal of Reproductive Immunology, 2022 BACKGROUND Various cytokines released by white blood cells like lymphocytes are linked to the immune system during pregnancy. The polymorphism of the TNFSF11 (rs2200287 and rs2148072) gene is related to the preeclampsia of pregnancy. MATERIALS AND METHODS This study was a prospective study involving 304 pregnant women with preeclampsia (n = 152) and controls (non-preeclamptic pregnant women) (n = 152). To investigate the rs2200287 and rs2148072 SNP's of TNFSF11 gene polymorphism by using the PCR - RFLP techniques. RESULTS A significantly different genotype distribution of TNFSF11 (rs2200287 and rs2148072) polymorphisms were observed between the two groups, with the G allele of variant rs2200287 was highly significant in the preeclamptic group (P = 000; 0.5814; OR = 0.5814; 95% CI = 0.4211 - 0.8012). And the C allele of variant rs2148072 was also highly significant in the preeclamptic group (P = 000; OR = 0.5076; 95% CI - 0.362 - 0.71) in this study. CONCLUSION The outcomes of the present study indicate that there was an association in TNFSF 11 (rs2200287 and rs2148072) gene polymorphism with preeclampsia compared to non - preeclampsia women. This article is protected by copyright. All rights reserved.
Association Between Red Cell Distribution Width and Glycated Hemoglobin in Type 2 Diabetes Mellitus: A Case-Control Study S Afzal, A Thomas, S Devulapalli, A Khare, VSBP Konakanchi, NA Kumar, ... Cureus 18 (4) , 2026 2026
Prevalence, Risk Factors, and Management Strategies of Hyperlipidemia Among Adolescents of a Tertiary Care Rural Indian Setting: A Cross-Sectional Study DL Lalitha, B Charishma, N Sivaraj, K Veerapaneni, LVS Kutikuppala, ... Cureus 17 (8) , 2025 2025
Study on MTHFR (C677T & A1298C) Gene Polymorphisms in the Condition of Glucose Intolerance During Pregnancy GV Bunga Papa Kusuma, D. Lakshmi Lalitha, Nagarjuna Sivaraj, Vijaya Sirisha ... American journal of reproductive immunology 93 (5), e70081 , 2025 2025
Health-care-seeking Behavior of Postmenopausal Women in an Urban Slum of Srikakulam Town P Roy, S Bose, TK Suvvari, N Sivaraj Apollo Medicine 21 (2), 127-131 , 2024 2024
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Association of MTHFD1 G1958A Polymorphism with Gestational Diabetes Mellitus AP Papa Kusuma Bunga , Vijaya Sirisha Balaga , Riya Raju , Tarun Kumar ... Cureus 16 (1), e53287 , 2024 2024 Citations: 2
Association of Tumor Necrosis Factor-Alpha (TNF-α) rs1800629 Polymorphism in Chronic Kidney Disease VRK Subhashini Mudapaka, A Arji, R Raju, TK Suvvari, N Sivaraj 2024
A Cross-Sectional Study to Evaluate the Apron Contamination among Health-Care Professionals of a Rural Tertiary Care Setting SH Boppanna, LVS Kutikuppala, N Sivaraj, KV Raman, B Radhika APIK Journal of Internal Medicine 11 (4), 237-239 , 2023 2023 Citations: 1
Prevalence of resistant hypertension and its relation with plasma lipoprotein‑associated phospholipase A2 NS Sri Harsha Boppana, Nikhil Reddy Ravula, L. V. Simhachalam Kutikuppala, C ... International Journal of Noncommunicable Diseases 8 (1), 46-50 , 2023 2023
Association of SNP rs163550 in IL5RA Gene with Risk of Preeclampsia N Sivaraj, K Vijaya Rachel, Tarun Kumar Suvvari , Shilaja Prasad , Sriharsha ... Journal of the Scientific Society 49 (3), 272-276 , 2022 2022
Association of TNFSF11 rs2200287 and TNFSF11 rs2148072 gene polymorphisms in preeclampsia N Sivaraj, BP Kusuma, LVS Kutikuppala, KV Rachel, VS Balaga, ... American Journal of Reproductive Immunology 88 (4), e13604 , 2022 2022 Citations: 4
Evaluation of the Association of Single Nucleotide Polymorphism rs2229238 in Interleukin 6 Receptor Alpha (IL6RA) Gene With the Risk of Preeclampsia N Sivaraj, Vijaya Rachel K, Tarun Kumar Suvvari, Shilaja Prasad, Boppana Sri ... Cureus 14 (5), 1-5 , 2022 2022 Citations: 1
Association of IL1R1 gene (SNP rs2071374) with the risk of preeclampsia N Sivaraj, KV Rachel, TK Suvvari, S Prasad, SH Boppana, PK Vegi Journal of Reproductive Immunology 149, 103463 , 2022 2022 Citations: 8
Antiviral Therapy for Herpes Simplex Virus Encephalitis: Systematic Review and Meta-Analysis of Randomized Control Trials S Amalakanti, SH Boppana, N Sivaraj, KVR Arepalli, TK Suvvari Journal of Applied Sciences and Clinical Practice 2 (3), 74-78 , 2021 2021
Cervical cancer metabolism: Major reprogramming of metabolic pathways and cellular energy production KV Rachel, N Sivaraj A Theranostic and Precision Medicine Approach for Female-Specific Cancers … , 2021 2021 Citations: 3
Assessment of protein carbonyl in type II diabetes mellitus in a tertiary care hospital NS G Ravi kumar1, S Hari Priya2 MedPulse International Journal of Biochemistry. 10 (2), 28-30. , 2019 2019
Incidence of HIV, HBV and HCV infections and association of ABO and Rh blood groups with these infections among blood donors in tertiary care hospital of coastal area of Andhra … S Konuri, K Rao, S Ausavi, N Sivaraj, M Siraz MedPulse International Journal of Microbiology 5 (3), 15-19 , 2018 2018 Citations: 2
Association of maternal angiotensinogen gene M235T polymorphism with preeclampsia in South India: A tertiary care hospital based case-control study K Chengalvala, P Kotur, M Shetty, P Kumar, J TV, N Sivaraj, ... Meta Gene 11, 108-110 , 2017 2017 Citations: 5
A study on evaluation of phospholipase A2 gene polymorphism and the expression in terms of phospholipase A2 enzyme in preeclampsia N Sivaraj 2017
Relationship of serum leptin, lactate dehydrogenase levels and severity in preeclampsia UD Kantipudi 2016 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Association of IL1R1 gene (SNP rs2071374) with the risk of preeclampsia N Sivaraj, KV Rachel, TK Suvvari, S Prasad, SH Boppana, PK Vegi Journal of Reproductive Immunology 149, 103463 , 2022 2022 Citations: 8
Antibacterial activity of flavonoids extracted from seeds of Pongamia pinnata Linn on methicillin resistant Staphylococcus aureus M Inala, C Dayanand, N Sivaraj, P Beena, A Kutty British Microbiology Research Journal 10 (1), 1-8 , 2015 2015 Citations: 7
Association of tumor necrosis Factor-Alpha (TNF-α) rs1800629 polymorphism in chronic kidney disease S Lanka, A Arji, R Raju, TK Suvvari, M Thakwani, BC Meenavilli, ... Cureus 16 (5) , 2024 2024 Citations: 6
Association of maternal angiotensinogen gene M235T polymorphism with preeclampsia in South India: A tertiary care hospital based case-control study K Chengalvala, P Kotur, M Shetty, P Kumar, J TV, N Sivaraj, ... Meta Gene 11, 108-110 , 2017 2017 Citations: 5
Association of TNFSF11 rs2200287 and TNFSF11 rs2148072 gene polymorphisms in preeclampsia N Sivaraj, BP Kusuma, LVS Kutikuppala, KV Rachel, VS Balaga, ... American Journal of Reproductive Immunology 88 (4), e13604 , 2022 2022 Citations: 4
Evaluation of maternal thyroid hormone status and atherogenic potency in pre eclampsia in Kolar District-A South West of India SR Sheela, CD Dayanand, N Sivaraj British Journal of Medicine and Medical Research 10 (4), 1-8 , 2015 2015 Citations: 4
Cervical cancer metabolism: Major reprogramming of metabolic pathways and cellular energy production KV Rachel, N Sivaraj A Theranostic and Precision Medicine Approach for Female-Specific Cancers … , 2021 2021 Citations: 3
Is ischemia modified albumin is a marker in Preeclampsia? A case control study in Kolar Population-a South West of India CD Dayanand 2015 Citations: 3
Association of MTHFD1 G1958A Polymorphism with Gestational Diabetes Mellitus AP Papa Kusuma Bunga , Vijaya Sirisha Balaga , Riya Raju , Tarun Kumar ... Cureus 16 (1), e53287 , 2024 2024 Citations: 2
Incidence of HIV, HBV and HCV infections and association of ABO and Rh blood groups with these infections among blood donors in tertiary care hospital of coastal area of Andhra … S Konuri, K Rao, S Ausavi, N Sivaraj, M Siraz MedPulse International Journal of Microbiology 5 (3), 15-19 , 2018 2018 Citations: 2
Relationship of serum leptin, lactate dehydrogenase levels and severity in preeclampsia UD Kantipudi 2016 Citations: 2
A Cross-Sectional Study to Evaluate the Apron Contamination among Health-Care Professionals of a Rural Tertiary Care Setting SH Boppanna, LVS Kutikuppala, N Sivaraj, KV Raman, B Radhika APIK Journal of Internal Medicine 11 (4), 237-239 , 2023 2023 Citations: 1
Evaluation of the Association of Single Nucleotide Polymorphism rs2229238 in Interleukin 6 Receptor Alpha (IL6RA) Gene With the Risk of Preeclampsia N Sivaraj, Vijaya Rachel K, Tarun Kumar Suvvari, Shilaja Prasad, Boppana Sri ... Cureus 14 (5), 1-5 , 2022 2022 Citations: 1
Association Between Red Cell Distribution Width and Glycated Hemoglobin in Type 2 Diabetes Mellitus: A Case-Control Study S Afzal, A Thomas, S Devulapalli, A Khare, VSBP Konakanchi, NA Kumar, ... Cureus 18 (4) , 2026 2026
Prevalence, Risk Factors, and Management Strategies of Hyperlipidemia Among Adolescents of a Tertiary Care Rural Indian Setting: A Cross-Sectional Study DL Lalitha, B Charishma, N Sivaraj, K Veerapaneni, LVS Kutikuppala, ... Cureus 17 (8) , 2025 2025
Study on MTHFR (C677T & A1298C) Gene Polymorphisms in the Condition of Glucose Intolerance During Pregnancy GV Bunga Papa Kusuma, D. Lakshmi Lalitha, Nagarjuna Sivaraj, Vijaya Sirisha ... American journal of reproductive immunology 93 (5), e70081 , 2025 2025
Health-care-seeking Behavior of Postmenopausal Women in an Urban Slum of Srikakulam Town P Roy, S Bose, TK Suvvari, N Sivaraj Apollo Medicine 21 (2), 127-131 , 2024 2024
Association of Tumor Necrosis Factor-Alpha (TNF-α) rs1800629 Polymorphism in Chronic Kidney Disease VRK Subhashini Mudapaka, A Arji, R Raju, TK Suvvari, N Sivaraj 2024
Prevalence of resistant hypertension and its relation with plasma lipoprotein‑associated phospholipase A2 NS Sri Harsha Boppana, Nikhil Reddy Ravula, L. V. Simhachalam Kutikuppala, C ... International Journal of Noncommunicable Diseases 8 (1), 46-50 , 2023 2023
Association of SNP rs163550 in IL5RA Gene with Risk of Preeclampsia N Sivaraj, K Vijaya Rachel, Tarun Kumar Suvvari , Shilaja Prasad , Sriharsha ... Journal of the Scientific Society 49 (3), 272-276 , 2022 2022
