Physicochemical and Functional Characterization of Pearl Millet-Based Probiotic Beverage for Antiaging Potential in Caenorhabditis elegans Nova Henna Jemimah Kaila, Prakash M. Halami, Chethana Ramakrishna, Mamatha Singanahalli Shivaramu, Muthukumar Serva Peddha Foods, 2025 Probiotics like Lactobacillus sp. are extensively studied for their beneficial host interactions, including the gut–brain axis, anti-inflammatory effects, immune system interactions, restoration of gut dysbiosis, and anti-aging effects. In the current study, pearl millet was fermented with Lactobacillus plantarum strains DHCU 70 and MCC 5231, which enhanced the nutritional, bioactive, and functional properties of derived probiotic beverages. Compared to unfermented controls, fermented beverages exhibited increased protein content and vitamins B1, B2, and B3, with decreased carbohydrate and dietary fiber levels. The probiotics have maintained viability exceeding 12 log CFU/mL and showed resistance to harsh gastrointestinal conditions. Fermentation increased total phenolic content from 13.38 ± 0.40 mg GAE/100 g to 42.10 ± 2.65 mg GAE/100 g (LPDB) and 47.76 ± 1.37 mg GAE/100 g (LPMB) and total flavonoid content from 13.01 ± 1.18 mg QE/100 g to 23.12 ± 2.73 mg QE/100 g and 24.21 ± 0.98 mg QE/100 g, respectively. Antioxidant assays showed DPPH radical scavenging improved by 37%, ferrous ion chelation rose from 71.69 ± 0.09 mg TE/100 g to 91.45 ± 0.006 mg TE/100 g, ABTS scavenging increased from 71.62 mg TE/100 g to 82.51 ± 0.04 mg TE/100 g (LPDB) and 89.74 ± 0.04 mg TE/100 g (LPMB) and superoxide radical inhibition rose from 51.40 ± 0.98% to 81.77 ± 0.03% (LPDB) and 79.92 ± 0.02% (LPMB). In the in vivo model, Caenorhabditis elegans, fermented beverage treatments significantly improved health-span parameters like head-swing frequency (13.51% increase), body bend frequency (8.41% increase), pharyngeal pumping (8.15% increase) with reduced lipofuscin accumulation and intracellular reactive oxygen species while median lifespan extended beyond 24 days versus 14–16 days in controls (p < 0.05). Gompertz mortality modeling revealed a significant decrease in the aging rate parameter, indicating systemic mitigation of stress-induced physiological decline. These combined nutritional, bioactive, and in vivo longevity results underscore the potential of L. plantarum-fermented pearl millet beverages as functional nutraceuticals that target oxidative stress and promote healthy aging.
Exploitation of freshwater fish waste as feedstock and Fenneropeneus indicus shrimp shell as catalyst source for biodiesel production Sandesh Suresh Karkal, Dhnyaneshwar Raising Rathod, Akil Salim Jamadar, Mamatha S. Shivaramu, Tanaji G. Kudre Biofuels, 2024 The current article emphasize on valorizing fishery waste such as Fenneropeneus indicus shrimp shell to derive catalyst for biodiesel production from freshwater fish waste (FFW) oil as an alternate solution to overcome environmental pollution. The catalyst characterization revealed that the catalyst derived by calcination of shrimp shell at 900 °C for 4h was crystalline, mesoporous and exhibited crystallite size of 42.28 nm, a surface area of 5.9861 m2/g and excellent basic strength (12.2< H < 15). As per the adopted central composite design (CCD) of response Surface Methodology (RSM), 1:11 mol/mol oil to methanol molar ratio, 6 wt% catalyst concentration, 65 °C reaction temperature, and 150 min reaction time were the optimum condition for maximum biodiesel yield of 82.57 wt%. The free fatty acid content (0.4%), acid value (0.84 mg KOH/g), iodine value (107 g I2/100 g), flash point (115 °C), and viscosity (7.48 mm2/s) ensured the application of biodiesel prepared from freshwater fish waste oil to be used as fuel. The physicochemical properties of biodiesel were evaluated to validate its feasibility. The FFW oil and calcined shrimp shell catalyst could be used as an alternate feedstock and catalyst source for sustainable biodiesel production and to reduce environmental pollution.
Prospects of novel heterogeneous base catalysts and nanocatalysts in achieving sustainable biodiesel production Dhnyaneshwar Raising Rathod, Sandesh Suresh Karkal, Akil Salim Jamadar, Aliaa M.A. Hashem, P. V Suresh, S.S Mamatha, Tanaji G. Kudre International Journal of Green Energy, 2024 The present review primarily focuses on the perspectives and state-of-the-art of heterogeneous catalysts, nanocatalysts, biocatalysts, bifunctional catalysts, metal-organic frameworks (MOF), and covalent organic frameworks (COF) for biodiesel production. The environmental concern associated with nonrenewable fossil fuels has led to finding alternative energy sources that can be used to meet global energy demands. Biofuels such as biodiesel are one of the energy sources that could replace fossil fuels. The homogeneous acid and base catalysts are generally used for commercial biodiesel production. However, homogeneous catalysts have downsides such as toxicity, corrosion, soap formation, high wastewater output, and non-reusability. Consequently, heterogeneous acid and base catalysts have been introduced that are less sensitive to moisture and free fatty acids (FFAs), easily separated and recovered, and reusable. Recently, novel catalysts such as waste biomass-derived mesoporous heterogeneous catalysts, chemically synthesized heterogeneous catalysts, metal ion-doped heterogeneous catalysts, bifunctional acid-base catalysts, and carbonaceous char-supported hetero catalysts, nanocatalysts, MOF and COF catalysts have potential to replace homogeneous base catalysts, aid in sustainable and cost-effective biodiesel production. This review provides insights into the recent advancement of various catalysts, catalyst preparation and operations, type of catalysts and suitability, catalyst efficiency, life cycle assessment, catalyst-associated challenges, and prospects for sustainable biodiesel production.
Comparative assessment of amino acids composition in two types of marine fish silage Mukund Gauthankar, Rakhee Khandeparker, Mamatha S. Shivaramu, Komal Salkar, Rayadurga Anantha Sreepada, Mandar Paingankar Scientific Reports, 2021 Fish silage is a brown liquefied product achieved by the action of enzymes when finely grounded whole/parts of either single or mixed fish types are subjected to acidification. This study made a comparative assessment of biochemical and nutritive properties, especially the amino acid composition in supernatant phase of formic acid silages prepared from two fish types, Indian mackerel (Rastrelliger kanagurta) and false travely (Lactarius lactarius) representing fat fish (FF, fat content > 5%) and lean fish (LF, fat content < 5%), respectively during 35 days of fermentation (DoF). Significantly higher content of total amino acid (TAA) and free amino acids (FAA) were recorded in FFS (TAA, 41.2 ± 0.03 mg/g; FAA, 31.3 ± 0.003 mg/g) compared to LFS (TAA, 35.8 ± 0.07 mg/g; FAA, 18.26 ± 0.003 mg/g; FAA, 31.3 ± 0.003 mg/g) (p < 0.05). At the end of 35 DoF, the concentrations of amino acids such as asparagine, histidine, isoleucine, valine, cysteine, serine, lysine and arginine were significantly higher in FFS as compared to LFS. The relative amino acid composition of FFS and LFS varied in accordance with DoF and the relationship was found to be highly significant (ANOVA, p < 0.00001). High concentrations of l-amino acids such as leucine, glutamic acid and arginine were recorded in both FFS and LFS. In conclusion, the analysis suggested that a fermentation period of 25–30 days showed a significant effect on the composition of amino acids in both types of ensilage compared to other fermentation periods (p < 0.05). Considering the role of amino acids in enhancing the plant growth and proliferation, the findings of the present study are quite useful.
Comparative assessment of antioxidant potential of different body parts of the yellow seahorse, Hippocampus kuda (Bleeker, 1852): Source of traditional medicine Indian Journal of Geo Marine Sciences, 2020
Implications of Microbial Thiosulfate Utilization in Red Clay Sediments of the Central Indian Basin: The Martian Analogy Tanya Singh, Pranav R. Kshirsagar, Anindita Das, Kunal Yadav, Sweta Mallik, M. B. L. Mascarenhas‐Pereira, Tresa Remya A. Thomas, Mamatha S. Shivaramu, LokaBharathi P. A., N. H. Khadge, B. Nagender Nath, Prashant K. Dhakephalkar, Sridhar D. Iyer, Dwijesh Ray, A. B. Valsangkar, Anita Garg, C. Prakash Babu, Ravindra J. Waghole, Shailesh S. Waghmare, Jyutika M. Rajwade, Kishore M. Paknikar Geochemistry Geophysics Geosystems, 2019
Sustainable insect pest management using bioactive compounds derived from marine macroalgae: A comprehensive review JA Varghese, ES Vendan, Y Rajashekar, MS Shivaramu Bioresource Technology, 134672 , 2026 2026
Physicochemical and Functional Characterization of Pearl Millet-Based Probiotic Beverage for Antiaging Potential in Caenorhabditis elegans NHJ Kaila, PM Halami, C Ramakrishna, MS Shivaramu, MS Peddha Foods 14 (20), 3460 , 2025 2025 Citations: 1
Dual-action of 2-Methyltetrahydrofuran-3-one (Coffee Furanone) as a natural fumigant and antimicrobial agent for stored grain protection CSVBYR S T. Suresh., M D. Gajendra. K H. Eraiah., J A. Varghese., M S ... Food Bioscience 74, 107943 , 2025 2025 Citations: 1
Characterization of fatty acid composition of healthy and diseased coral mucus released from host and/or endosymbiont for their possible role in coral health PV Bhagwat, C Ravindran, V Keerthana, SS Mamatha Marine Biology 172 (1), 19 , 2025 2025 Citations: 2
Prospects of novel heterogeneous base catalysts and nanocatalysts in achieving sustainable biodiesel production D Raising Rathod, S Suresh Karkal, AS Jamadar, AMA Hashem, ... International Journal of Green Energy 21 (5), 1017-1042 , 2024 2024 Citations: 23
Exploitation of freshwater fish waste as feedstock and Fenneropeneus indicus shrimp shell as catalyst source for biodiesel production SS Karkal, DR Rathod, AS Jamadar, MS Shivaramu, TG Kudre Biofuels 15 (1), 1-15 , 2024 2024 Citations: 11
Production optimization, scale-up, and characterization of biodiesel from marine fishmeal plant oil using Portunus sanguinolentus crab shell derived heterogeneous catalyst SS Karkal, DR Rathod, AS Jamadar, SS Mamatha, TG Kudre Biocatalysis and Agricultural Biotechnology 47, 102571 , 2023 2023 Citations: 29
Comparative assessment of amino acids composition in two types of marine fish silage M Gauthankar, R Khandeparker, MS Shivaramu, K Salkar, RA Sreepada, ... Scientific Reports 11 (1), 15235 , 2021 2021 Citations: 17
Chemoautotrophy at the Expense of Thiosulphate: A Supplementary Nutritional Source to the Mangrove Clam-Polymesoda Erosa TRA Thomas, T Singh, TS Kalaimani, NH Khadge, MS Shivaramu, ... 2021 Citations: 1
Evaluation of the physiological bacterial groups in a tropical biosecured, zero-exchange system growing whiteleg shrimp, Litopenaeus vannamei EA Sabu, MJ Gonsalves, RA Sreepada, MS Shivaramu, N Ramaiah Microbial ecology 81 (2), 335-346 , 2021 2021 Citations: 5
Characterization of coral associated ciliates and their interactions with disease lesion progression of Indian scleractinian corals C Ravindran, PV Bhagwat, KA Silveira, MS Shivaramu Microbial pathogenesis 149, 104472 , 2020 2020 Citations: 12
Comparative assessment of antioxidant potential of different body parts of the yellow seahorse, Hippocampus kuda (Bleeker, 1852): Source of traditional medicine SV Sanaye, AP Pawar, NM Pise, RA Sreepada, SS Mamatha, HB Pawar Indian J Mar Sci 49 (5), 804-811 , 2020 2020 Citations: 1
Morphological and molecular evidence for first records and range extension of the Japanese seahorse, Hippocampus mohnikei (Bleeker 1853) in a bay-estuarine … SV Sanaye, R Khandeparker, AS Rayadurga, MS Shivaramu, ... PLoS One 15 (3), e0220420 , 2020 2020 Citations: 9
Preliminary study on the response of marine fungoid protists, the thraustochytrids, to lipid extracts of diatoms VS Damare, PM D’Costa, MS Shivaramu, V Borges, M Fernandes, ... Aquatic Ecology 54 (1), 355-367 , 2020 2020 Citations: 5
Dynamics and succession of plankton communities with changing nutrient levels in tropical culture ponds of whiteleg shrimp V Fernandes, EA Sabu, MS Shivaramu, MJBD Gonsalves, RA Sreepada Aquaculture Environment Interactions 11, 639-655 , 2019 2019 Citations: 32
Morphological and molecular evidence for range extension and first occurrence of the Japanese seahorse, Hippocampus mohnikei (Bleeker 1853) in a bay … S Sanaye, R Khandeparker, RA Sreepada, MS Shivaramu, H Kankonkar, ... bioRxiv, 705814 , 2019 2019
Improvement in biomass, lipid production and biodiesel properties of a euryhaline Chlorella vulgaris NIOCCV on mixotrophic cultivation in wastewater from a fish processing plant T Trivedi, D Jain, NSS Mulla, SS Mamatha, SR Damare, RA Sreepada, ... Renewable energy 139, 326-335 , 2019 2019 Citations: 43
Diversity and extracellular enzyme activities of heterotrophic bacteria from sediments of the Central Indian Ocean Basin VS Gawas, MS Shivaramu, SR Damare, D Pujitha, RM Meena, ... Scientific Reports 9 (1), 9403 , 2019 2019 Citations: 20
Implications of microbial thiosulfate utilization in red clay sediments of the Central Indian Basin: the Martian analogy T Singh, PR Kshirsagar, A Das, K Yadav, S Mallik, ... Geochemistry, Geophysics, Geosystems 20 (2), 708-729 , 2019 2019 Citations: 7
CO2 fixation and production of biodiesel by Chlorella vulgaris NIOCCV under mixotrophic cultivation D Jain, SS Ghonse, T Trivedi, GL Fernandes, LD Menezes, SR Damare, ... Bioresource technology 273, 672-676 , 2019 2019 Citations: 79
MOST CITED SCHOLAR PUBLICATIONS
CO2 fixation and production of biodiesel by Chlorella vulgaris NIOCCV under mixotrophic cultivation D Jain, SS Ghonse, T Trivedi, GL Fernandes, LD Menezes, SR Damare, ... Bioresource technology 273, 672-676 , 2019 2019 Citations: 79
Medium Optimization of Gamma Linolenic Acid Production in Mucor rouxii CFR -G15 using RSM SS Mamatha, R Ravi, G Venkateswaran Food and Bioprocess Technology 1 (4), 405-409 , 2008 2008 Citations: 67
Improvement in biomass, lipid production and biodiesel properties of a euryhaline Chlorella vulgaris NIOCCV on mixotrophic cultivation in wastewater from a fish processing plant T Trivedi, D Jain, NSS Mulla, SS Mamatha, SR Damare, RA Sreepada, ... Renewable energy 139, 326-335 , 2019 2019 Citations: 43
Dynamics and succession of plankton communities with changing nutrient levels in tropical culture ponds of whiteleg shrimp V Fernandes, EA Sabu, MS Shivaramu, MJBD Gonsalves, RA Sreepada Aquaculture Environment Interactions 11, 639-655 , 2019 2019 Citations: 32
Production optimization, scale-up, and characterization of biodiesel from marine fishmeal plant oil using Portunus sanguinolentus crab shell derived heterogeneous catalyst SS Karkal, DR Rathod, AS Jamadar, SS Mamatha, TG Kudre Biocatalysis and Agricultural Biotechnology 47, 102571 , 2023 2023 Citations: 29
Prospects of novel heterogeneous base catalysts and nanocatalysts in achieving sustainable biodiesel production D Raising Rathod, S Suresh Karkal, AS Jamadar, AMA Hashem, ... International Journal of Green Energy 21 (5), 1017-1042 , 2024 2024 Citations: 23
Diversity and extracellular enzyme activities of heterotrophic bacteria from sediments of the Central Indian Ocean Basin VS Gawas, MS Shivaramu, SR Damare, D Pujitha, RM Meena, ... Scientific Reports 9 (1), 9403 , 2019 2019 Citations: 20
Identification and characterization of the n ‐6 fatty acid‐producing Mucor rouxii native isolate CFR‐G15 SS Mamatha, PM Halami, G Venkateswaran European journal of lipid science and technology 112 (3), 380-389 , 2010 2010 Citations: 19
Comparative assessment of amino acids composition in two types of marine fish silage M Gauthankar, R Khandeparker, MS Shivaramu, K Salkar, RA Sreepada, ... Scientific Reports 11 (1), 15235 , 2021 2021 Citations: 17
Assessment of physicochemical and bacteriological drinking water quality of different sources of HD Kote town Mysore district C Roopavathi, SS Mamatha, NS Raju Int J Eng Res Appl, 6 (7), 45-51 , 2016 2016 Citations: 16
Polyunsaturated fatty acids (PUFAs) of Mucor sp. with special reference to gamma linolenic acid (GLA) SS Mamatha University of Mysore , 2009 2009 Citations: 14
Interactions between trophic levels in upwelling and non-upwelling regions during summer monsoon A Malik, CEG Fernandes, MJBD Gonsalves, NS Subina, SS Mamatha, ... Journal of sea research 95, 56-69 , 2015 2015 Citations: 13
Characterization of coral associated ciliates and their interactions with disease lesion progression of Indian scleractinian corals C Ravindran, PV Bhagwat, KA Silveira, MS Shivaramu Microbial pathogenesis 149, 104472 , 2020 2020 Citations: 12
Alkaline phosphatase activity at the southwest coast of India: A comparison of locations differently affected by upwelling SS Mamatha, A Malik, S Varik, V Parvathi, VK Jineesh, MU Gauns, ... Journal of Sea Research 95, 196-205 , 2015 2015 Citations: 12
Exploitation of freshwater fish waste as feedstock and Fenneropeneus indicus shrimp shell as catalyst source for biodiesel production SS Karkal, DR Rathod, AS Jamadar, MS Shivaramu, TG Kudre Biofuels 15 (1), 1-15 , 2024 2024 Citations: 11
Phosphate solubilizing bacteria and alkaline phosphatase activity in coastal waters off Trivandrum SS Mamatha, A Gobika, P Janani Centre for Ocean and Environmental Studies, New Delhi , 2012 2012 Citations: 11
Morphological and molecular evidence for first records and range extension of the Japanese seahorse, Hippocampus mohnikei (Bleeker 1853) in a bay-estuarine … SV Sanaye, R Khandeparker, AS Rayadurga, MS Shivaramu, ... PLoS One 15 (3), e0220420 , 2020 2020 Citations: 9
Implications of microbial thiosulfate utilization in red clay sediments of the Central Indian Basin: the Martian analogy T Singh, PR Kshirsagar, A Das, K Yadav, S Mallik, ... Geochemistry, Geophysics, Geosystems 20 (2), 708-729 , 2019 2019 Citations: 7
Spatiotemporal variation of alkaline phosphatase activity in coastal waters off Trivandrum MS Shivaramu, AK Randive, R Kumari, M Gauns, LBA Ponnapakkam Oceanologia 61 (1), 170-177 , 2019 2019 Citations: 7
Astrobiological implications of dim light phototrophy in deep-sea red clays A Das, T Singh, PA LokaBharathi, PK Dhakephalkar, S Mallik, ... Life sciences in space research 12, 39-50 , 2017 2017 Citations: 6
