Functional and Histological Analysis of Stem Cell and Amniotic Membrane Implantation After Acute Myocardial Infarction with Left Ventricular Dysfunction: Experimental Study Isabella Cristina Mendes Rossa, Marcos Antônio Denk, Luize Kremer Gamba, Anna Clara Faidiga Silva, Julia Letícia de Bortolo, et al. International Journal of Molecular Sciences, 2026 Acute myocardial infarction (AMI) results from a lack of oxygen supply to the myocardium, leading to the loss of cardiomyocytes and their replacement with fibrotic scar tissue. This process is closely associated with the development of heart failure. Regenerative medicine has emerged as a promising strategy to enhance treatment outcomes in severe cases of heart failure. This study aimed to evaluate myocardial regeneration after AMI using a biomaterial composed of mononuclear stem cells and human amniotic membrane. A total of 120 Wistar rats were subjected to experimentally induced AMI. On the 7th day post-infarction, rats with an ejection fraction of <50% on echocardiography were randomized into four groups: (1) control; (2) stem cells; (3) amniotic membrane; and (4) amniotic membrane combined with stem cells. On the 30th day, the surviving animals underwent a second echocardiographic evaluation and were subsequently euthanized. The group treated with the combination of amniotic membrane and stem cells showed reduced systolic and diastolic ventricular volumes. Histological analysis revealed that these animals exhibited less fibrosis and a lower percentage of type I collagen. Based on the results of the study, it was concluded that the combination of human amniotic membrane and mononuclear stem cells decreased ventricular volumes and myocardial fibrosis, suggesting more favorable ventricular remodeling in this experimental model.
SM3DD with segmented PCA: A comprehensive method for interpreting 3D spatial transcriptomics Tony Blick, Aaron Kilgallon, James Monkman, Caroline Cooper, Chin Wee Tan, et al. Nar Genomics and Bioinformatics, 2026 We developed Standardised Minimum 3D Distance (SM3DD), an entirely cell segmentation/annotation-free approach to the analysis of spatial RNA datasets, using it to compare lung tissue from 16 clinically normal individuals to that of 18 SARS-CoV-2 patients who died from acute respiratory distress syndrome. RNA spatial coordinates were determined using the CosMx™ Spatial Molecular Imager (Bruker Spatial Biology, US). For each individual transcript location, we calculated the three-dimensional distances to the nearest transcript of each transcript type, standardising the distances to each transcript type. Mean SM3DDs were compared between normal and SARS-CoV-2 patients. Notably, hierarchical clustering of the directional log10(P) values organized genes by functionality, making it easier to interpret biological contexts, and for FKBP11, where a decrease in distance to MZT2A was the most significant difference, suggesting a role in interferon signalling. Using a segmented principal components analysis of the entire SM3DD dataset, we identified multiple pathways, including ‘SARS-CoV-2 infection’, even though the assay did not include any SARS-CoV-2 transcripts.
Effects of maternal arginine supplementation during lactation on offspring growth and wound healing in an experimental rat model of intrauterine malnutrition Ayrton Alves Aranha Junior, Jorge Eduardo Fouto Matias, Djanira Aparecida da Luz Veronez, Lucia de Noronha, João Carlos Repka, et al. Acta Cirurgica Brasileira, 2026 Purpose: To evaluate the effects of maternal arginine-enriched diet during lactation, following gestational undernutrition, on offspring growth and wound healing. Methods: Pregnant Wistar rats were divided into two groups during gestation: control (C; ad libitum diet) and undernourished (Und; 60% of control diet). After delivery, the undernourished mothers were reassigned to two subgroups: ad libitum + Impact® (Arg) and ad libitum + Nutren® (Hh). Evaluated parameters included offspring anthropometric measurements, serum albumin, tensile strength, and collagen morphometry at the surgical wound. Statistical analyses included analysis of variance (ANOVA) and Tukey’s, Student’s t-test, and Fisher’s test. Results: Maternal immunonutrition during lactation promoted recovery of offspring weight (C = 29.21 ± 2.86 g; Arg = 36.08 ± 5.38 g; p = 0.0009) and body length (C = 9.87 ± 0.32 cm; Arg = 10.91 ± 0.73 cm; p = 0.0008), shifting values from significantly lower at birth to higher than controls by weaning. Offspring healing parameters showed no significant differences compared with controls. Conclusion: An arginine-enriched diet provided to lactating rats malnourished during gestation significantly improved offspring anthropometric growth and supported wound healing following surgery at weaning.
Histopathological evaluation in post-mortem renal biopsies of patients with covid-19 and comorbidities: A case-control study Samya Hamad Mehanna, Mayara Pezzini Arantes, Henrique Machado de Souza Proença, Rafael Weissheimer, Sérgio Ossamu Ioshii, et al. Sao Paulo Medical Journal, 2026 BACKGROUND: Acute kidney injury is one of the main systemic complications of severe coronavirus disease 2019 (COVID-19). OBJECTIVES: To examine histopathological changes in post-mortem kidney biopsies of patients who died as a result of the disease caused by SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). DESIGN AND SETTING: A case-control study was conducted at a tertiary hospital located in Curitiba, Paraná, Brazil. METHODS: The study group, called "COVID," consisted of kidney biopsy samples obtained from deceased patients with COVID-19, with a "Control" group included for comparison. Samples were selected based on sex, age, and comorbidities, with an emphasis on diabetes mellitus and systemic arterial hypertension (SAH). Morphological evaluation was performed by pathologists using preestablished criteria with glomerular, tubular, and vascular characteristics among the parameters. RESULTS: Tubular atrophy and interstitial fibrosis, markers of chronic kidney injury, were observed with equal frequency in both groups, probably because of the initial pairing of the samples. These findings are in line with what would be expected from chronic exposure to proteinuria. In relation to SAH, the main identification was interstitial vascular damage, particularly arteriolosclerosis/arteriosclerosis. Acute tubular injury was the most frequently observed feature in patients in the COVID group, which was probably related to ischemic damage. CONCLUSION: This study demonstrated that the main change identified in the renal parenchyma of patients with COVID-19 was acute tubular injury, which was expected considering the context of severe systemic ischemia to which these patients are subjected, with the other findings being the consequences of chronic damage.
Chikungunya virus-induced persistent allodynia is accompanied by joint microenvironmental changes and involves cartilage degradation and TRPA1 activation Liziane C. M. da Silva, Andressa C. dos Santos Maia, Nágila C. F. de Sousa, Catielen P. Pavi, Beatriz P. Savi, et al. British Journal of Pharmacology, 2026 Background and purpose Chikungunya virus (CHIKV) causes debilitating chronic joint pain. CHIKV‐induced pain mechanisms are not completely understood and models designed to resemble CHIKV chronic pain are critical for the development of novel therapeutic approaches. Experimental approach Mechanical allodynia was induced by injection of either inactivated CHIKV or its viral RNA into the mouse ipsilateral knee. Both ipsilateral and contralateral joints were collected for histological analysis, collagen Type I content and immunohistochemistry. The effects of paracetamol and sodium diclofenac were assessed and the role of the transient receptor potential ankyrin 1 (TRPA1) was investigated using the antagonist HC‐030031 and TRPA1 knockout (KO) mice. CHIKV‐induced Ca 2+ influx was also assessed in mouse dorsal root ganglion (DRG) neurones. Key results CHIKV or its RNA caused pronounced and time‐dependent bilateral secondary mechanical allodynia. CHIKV caused structural damage in the ipsilateral joint and increased collagen type I content. Higher IL‐10, VEGF and TGF β expressions were observed in the affected joints. Paracetamol or sodium diclofenac reduced allodynia, which was also absent in TRPA1 KO and HC‐030031‐treated mice. TRPA1 expression was up‐regulated in CHIKV‐injected joints whilst CHIKV envelope E2 protein or RNA induced Ca 2+ influx in DRG neurones was antagonized by HC‐030031. Conclusion and implications Insights into CHIKV‐associated pain have been made giving a guide to targeted analgesic strategies. CHIKV or its RNA injection triggers bilateral secondary mechanical allodynia, accompanied by knee joint damage and a TRPA1‐dependent mechanism. Indicating TRPA1 blockers have the potential to be repurposed or further investigated for the clinical management of CHIKV‐associated chronic pain.
Expression of Parkin, APC, APE1, and Bcl-xL in Colorectal Polyps Rosimeri Kühl Svoboda Baldin, Carmen Austrália Paredes Marcondes Ribas, Lúcia de Noronha, Claudia Caroline Veloso da Silva-Camargo, Vanessa Santos Sotomaior, et al. Journal of Histochemistry and Cytochemistry, 2021
Intrauterine transmission of SARS-CoV-2 Emanuele T. S. Stonoga, Laura de Almeida Lanzoni, Patricia Zadorosnei Rebutini, André Luiz Permegiani de Oliveira, Jullie Anne Chiste, et al. Emerging Infectious Diseases, 2021
Zika virus and pregnancy: An overview Newton Sérgio de Carvalho, Beatriz Freitas de Carvalho, Bruna Dóris, Evellyn Silverio Biscaia, Cyllian Arias Fugaça, et al. American Journal of Reproductive Immunology, 2017
Bone marrow derived mononuclear stromal cells and experimental model of deep endometriosis International Journal of Clinical and Experimental Pathology, 2016
Obesity and abdominal wound healing in rats Maria de Lourdes Pessole Biondo-Simões, Guilherme Roberto Zammar, Rodrigo dos Santos Fernandes, Rachel Biondo-Simões, Flavia Stica Ritzdorf de Mello, et al. Acta Cirurgica Brasileira, 2010
Histopathological and morphometric analysis in the diagnosis of "new" broncopulmonar dysplasia and clinical and pathological comparison with the classic form of the disease Jornal Brasileiro De Patologia E Medicina Laboratorial, 2009
Griscelli syndrome and electroencephalography pattern Simone C. Vieira-Karuta, Izabella C. Bertoldo Silva, Nádia Aparecida P. Almeida, Lúcia de Noronha, Mara Lúcia S.F. Santos, et al. Arquivos De Neuro Psiquiatria, 2008
Abdominal wall healing in reoperated rats Zacarias Alves de Souza Filho, Fernando Hintz Greca, Lúcia de Noronha, André Souza de Albuquerque Maranhão, Ana Paula Calil, et al. Acta Cirurgica Brasileira, 2007
Vascular radiolesion as a deleterious effect of high-dose-rate intraarterial brachytherapy with samarium-153 in hypercholesterolemic rabbits Arquivos Brasileiros De Cardiologia, 2006
Comparative study of histopathological abnormalities induced by CO2 and erbium laser on the skin of wistar rats Jornal Brasileiro De Patologia E Medicina Laboratorial, 2001
Subcutaneous panniculitic T cell lymphoma: A case report affecting a child Jornal Brasileiro De Patologia E Medicina Laboratorial, 2001
April 2001: A 70 year old woman with recurrent meningioma Brain Pathology, 2001
Epidemiological study of 662 pediatric cases of skin lesions, with histological analysis and revision of literature Anais Brasileiros De Dermatologia, 2001
Optic nerve gliomas: A study of 11 cases Luiz Fernando Bleggi Torres, Bruno Carneiro De Medeiros, Lúcia De Noronha, Ari Pedroso, Ricardo Ramina Arquivos De Neuro Psiquiatria, 1996
Sacrococcygeal chordoma in a 9-year-old boy. Lúcia de Noronha, Betina Werner, Carmem Maria C. Mendonça, Luiz Nomura, Luiz Fernando Bleggi-Torres Arquivos De Neuro Psiquiatria, 1995
