Kelly Cristine Borsatto

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Kelly Cristine Borsatto


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https://researchid.co/kellyborsatto
Bachelor in Biological Sciences from the São Paulo State University (UNESP) Júlio de Mesquita Filho on the campus of São José do Rio Preto, São Paulo (IBILCE). He is currently developing a doctoral project entitled: Analysis of the Parasite-Vector Interaction of Chagas Disease - Metabolic Profile – (FAPESP Scholarship), under the guidance of Professor Raghuvir Krishnaswamy Arni at the Graduate Program in Microbiology at IBILCE/UNESP.

RESEARCH, TEACHING, or OTHER INTERESTS

Cell Biology, Insect Science, Molecular Biology, Parasitology
13

Scopus Publications

95

Scholar Citations

6

Scholar h-index

3

Scholar i10-index

Scopus Publications

  • Omics Tools Applied to the Study of Chagas Disease Vectors: Proteomics and Metabolomics
    Kelly Cristine Borsatto, Monika Aparecida Coronado, Cleber Galvão, Raghuvir Krishnaswamy Arni, Kaio Cesar Chaboli Alevi
    American Journal of Tropical Medicine and Hygiene, 2025
    Recent omics studies (cytogenomics, genomics, and transcriptomics) have provided extensive information on Chagas disease (CD) vectors. Here, we conduct a review of proteomic and metabolomic studies on these insect vectors. We address proteomic studies in triatomines, focusing on the identification and characterization of proteins in their saliva, midgut, and salivary glands, emphasizing their role in blood feeding by providing vasodilatory and anticoagulant substances, along with anti-inflammatory and antiplatelet activities. In addition, the review investigates protein diversity in different triatomine species, highlighting the importance of proteomic analysis in understanding adaptation to hematophagy, genetic evolution, and vector defense mechanisms. Metabolomic studies on CD vectors have revealed crucial information about their biology because metabolites correlate with the presence of Trypanosoma cruzi (T. cruzi), suggesting roles in the parasite–vector interaction. Additionally, some studies have predicted T. cruzi infection and observed altered metabolomes after feeding on infected blood, providing information about vector exposure to the parasite.
  • HR-MAS NMR Metabolomics Profile of Vero Cells under the Influence of Virus Infection and nsP2 Inhibitor: A Chikungunya Case Study
    Rafaela dos S. Peinado, Lucas G. Martins, Carolina C. Pacca, Marielena V. Saivish, Kelly C. Borsatto, et al.
    International Journal of Molecular Sciences, 2024
    The arbovirus Chikungunya (CHIKV) is transmitted by Aedes mosquitoes in urban environments, and in humans, it triggers debilitating symptoms involving long-term complications, including arthritis and Guillain-Barré syndrome. The development of antiviral therapies is relevant, as no efficacious vaccine or drug has yet been approved for clinical application. As a detailed map of molecules underlying the viral infection can be obtained from the metabolome, we validated the metabolic signatures of Vero E6 cells prior to infection (CC), following CHIKV infection (CV) and also upon the inclusion of the nsP2 protease inhibitor wedelolactone (CWV), a coumestan which inhibits viral replication processes. The metabolome groups evidenced significant changes in the levels of lactate, myo-inositol, phosphocholine, glucose, betaine and a few specific amino acids. This study forms a preliminary basis for identifying metabolites through HR-MAS NMR (High Resolution Magic Angle Spinning Nuclear Magnetic Ressonance Spectroscopy) and proposing the affected metabolic pathways of cells following viral infection and upon incorporation of putative antiviral molecules.
  • First evidence of gonadal hybrid dysgenesis in Chagas disease vectors (Hemiptera, Triatominae): gonad atrophy prevents events of interspecific gene flow and introgression
    Luísa Martins Sensato Azevedo, Natália Regina Cesaretto, Jader de Oliveira, Amanda Ravazi, Yago Visinho dos Reis, et al.
    Parasites and Vectors, 2023
    Background Hybridization events between Triatoma spp. have been observed under both natural and laboratory conditions. The ability to produce hybrids can influence different aspects of the parent species, and may even result in events of introgression, speciation and extinction. Hybrid sterility is caused by unviable gametes (due to errors in chromosomal pairing [meiosis]) or by gonadal dysgenesis (GD). All of the triatomine hybrids analyzed so far have not presented GD. We describe here for the first time GD events in triatomine hybrids and highlight these taxonomic and evolutionary implications of these events. Methods Reciprocal experimental crosses were performed between Triatoma longipennis and Triatoma mopan. Intercrosses were also performed between the hybrids, and backcrosses were performed between the hybrids and the parent species. In addition, morphological and cytological analyzes were performed on the atrophied gonads of the hybrids. Results Hybrids were obtained only for the crosses T. mopan♀ × T. longipennis♂. Intercrosses and backcrosses did not result in offspring. Morphological analyses of the male gonads of the hybrids confirmed that the phenomenon that resulted in sterility of the hybrid was bilateral GD (the gonads of the hybrids were completely atrophied). Cytological analyses of the testes of the hybrids also confirmed GD, with no germ cells observed (only somatic cells, which make up the peritoneal sheath). Conclusions The observations made during this study allowed us to characterize, for the first time, GD in triatomines and demonstrated that gametogenesis does not occur in atrophied gonads. The characterization of GD in male hybrids resulting from the crossing of T. mopan♀ × T. longipennis♂ highlights the importance of evaluating both the morphology and the cytology of the gonads to confirm which event resulted in the sterility of the hybrid: GD (which results in no gamete production) or meiotic errors (which results in non-viable gametes). Graphical Abstract
  • Morphological, Cytological and Molecular Studies and Feeding and Defecation Pattern of Hybrids from Experimental Crosses between Triatoma sordida and T. rosai (Hemiptera, Triatominae)
    Roberto Dezan Vicente, Fernanda Fernandez Madeira, Kelly Cristine Borsatto, Ariane Cristina Caris Garcia, Daniel Cesaretto Cristal, et al.
    Pathogens, 2022
    Under laboratory conditions, Triatoma rosai and T. sordida are able to cross and produce hybrids. In the face of climate and environmental changes, the study of hybrids of triatomines has evolutionary and epidemiological implications. Therefore, we performed morphological, cytological and molecular studies and characterized the feeding and defecation pattern of hybrids from crosses between T. sordida and T. rosai. The morphological characterization of the female genitalia of the hybrids showed that characteristics of both parental species segregated in the hybrids. Cytogenetic analyzes of hybrids showed regular metaphases. According to molecular studies, the mitochondrial marker Cytochrome B (CytB) related the hybrids with T. sordida and the nuclear marker Internal Transcribed Spacer 1 (ITS-1) related the hybrids with T. rosai. Both parents and hybrids defecated during the blood meal. Thus, the hybrids resulting from the cross between T. sordida and T. rosai presented segregation of phenotypic characters of both parental species, 100% homeology between homeologous chromosomes, phylogenetic relationship with T sordida and with T. rosai (with CytB and ITS-1, respectively), and, finally, feeding and defecation patterns similar to the parents.
  • Transcriptomics Applied to the Study of Chagas Disease Vectors
    Kelly Cristine Borsatto, Monika Aparecida Coronado, Cleber Galvão, Raghuvir Krishnaswamy Arni, Kaio Cesar Chaboli Alevi
    American Journal of Tropical Medicine and Hygiene, 2022
    Chagas disease is a neglected disease caused by the protozoan Trypanosoma cruzi, and is transmitted mainly by the feces of contaminated triatomines. Knowledge of the biological, ecological, behavioral, genetic, taxonomic, and systematic aspects of these vectors can contribute to the planning of vector control programs, because all species are considered to be potential vectors of Chagas disease. Transcriptomic studies, in general, provided a new view of the physiology of triatomines (aiding in the knowledge of reproductive aspects of the hematophagy process and even the immune system and the sensory apparatus) and even contributed, as a new tool, to the taxonomy and systematics of these insects. Thus, we conducted a review of the transcriptomic studies on Chagas disease vectors.
  • Omics tools applied to the study of chagas disease vectors: Cytogenomics and genomics
    Kelly Cristine Borsatto, Monika Aparecida Coronado, Raghuvir Krishnaswamy Arni, Chaboli Alevi Kaio Cesar
    American Journal of Tropical Medicine and Hygiene, 2021
    Chagas disease is an illness caused by the protozoan Trypanosoma cruzi that is distributed in 21 countries of Latin America. The main way of transmission of T. cruzi is through the feces of triatomines infected with the parasite. With technological advances came new technologies called omics. In the pre-genomic era, the omics science was based on cytogenomic studies of triatomines. With the Rhodnius prolixus genome sequencing project, new omics tools were applied to understand the organism at a systemic level and not just from a genomic point of view. Thus, the present review aims to put together the cytogenomic and genomic information available in the literature for Chagas disease vectors. Here, we review all studies related to cytogenomics and genomics of Chagas disease vectors, contributing to the direction of further research with these insect vectors, because it was evident that most studies focus on cytogenomic knowledge of the species. Given the importance of genomic studies, which contributed to the knowledge of taxonomy, systematics, as well as the vector’s biology, the need to apply these techniques in other genera and species of Triatominae subfamily is emphasized.
  • Cytotaxonomy of dipetalogaster maxima uhler, 1894 (Hemiptera, reduviidae, triatominae)
    K. C. Borsatto, K. C. C. Alevi, J. Oliveira, M. T. V. Azeredo-Oliveira
    Brazilian Journal of Biology, 2020
    The Triatomini tribe consists of ten genera and is regarded as one of the most important tribes from epidemiological point of view. The genus Dipetalogaster Usinger, 1939 is composed only by the species Dipetalogaster maxima Uhler, 1894. This triatomine is exclusive of the Mexico and is a potential vector for Chagas disease. Besides the epidemiological importance, the insects of the Triatominae subfamily are important biological models for cytogenetic studies. Therefore, in order to contribute to the knowledge on the reproductive biology and assist in citotaxonomy of D. maxima, this study aimed to describe spermatogenesis, as well as confirm the karyotype and heterochromatic patterns of this Mexican triatomine species. The seminiferous tubules were torn, fixed to a cover slip and underwent the cytogenetic technique of Lacto-acetic orcein and C-banding. Through the cytogenetics analysis of testicular material D. maxima it was possible to confirm the karyotype (2n = 22), describe the stages of spermatogenesis and characterize the heterochromatic pattern (restricted to sex chromosome Y) of the species. D. maxima showed the same arrangement of heterochromatin described for Triatoma lecticularia (Stål, 1859) (a species that occur in United States of American and Mexico and is phylogenetically related with D. maxima), highlighting the importance of this analysis as an optimization tool to explore phylogenetic correlations.
  • Identification key for the chagas disease vectors of five brazilian states, based on cytogenetic data
    Kelly Cristine Borsatto, Maria Tercília Vilela Azeredo-Oliveira, Kaio Cesar Chaboli Alevi
    American Journal of Tropical Medicine and Hygiene, 2019
    Chagas disease is a public health problem caused by the protozoan Trypanosoma cruzi that affects about 8 million people worldwide. The main form of transmission of T. cruzi is vectorial, through triatomines feces contaminated with the parasite. All species are considered as potential vectors of T. cruzi. The main identification keys of these vectors are based only on morphological characters. However, there are very similar or even same species (cryptic species) that may lead to wrong classification of the vectors. Therefore, we developed an identification key using cytogenetic data, to aid and help the correct classification of triatomines. From the cytogenetic characters, identification keys were created for the five Brazilian states (Alagoas, Amapá, Ceará, Roraima, and Santa Catarina). These data are important because the correct classification of triatomines helps directly the activity of the vector control programs.
  • CytoKey: Identification key for the chagas disease vectors of the Largest Brazilian Urban Center (São Paulo State), based on cytogenetic data
    Kelly Cristine Borsatto, Yago Visinho dos Reis, Ariane Cristina Caris Garcia, Paulo Sergio de Sousa, Maria Tercília Vilela de Azeredo-Oliveira, et al.
    American Journal of Tropical Medicine and Hygiene, 2019
    Chagas disease is caused by the protozoan Trypanosoma cruzi. This disease is distributed in 21 Latin American countries, where it is mostly vector-borne. In Brazil, there are 68 triatomine species. To date, the epidemiological surveys indicate that the state of São Paulo presents 11 species of triatomines, and most of these species have already been collected in a home environment and found to be infected with T. cruzi. Problems in correctly identifying species can lead to incorrect panorama of distribution of Chagas disease vectors. Thus, we developed an identification key for the triatomines of the state of São Paulo, based on cytogenetic data. With the exception of Panstrongylus diasi that does not present cytogenetic data available in the literature, all species were differentiated by cytogenetic characteristics. We emphasize the importance of using this key as a simple and objective tool in the entoepidemiological surveys conducted by the vector control programs.
  • Revisiting the homoploid hybrid speciation process of the triatoma brasiliensis macromelasoma galvão, 1956 (hemiptera, triatominae) using cytogenetic and molecular markers
    Ana Letícia Guerra, Kelly Cristine Borsatto, Nicolle Pagliusi Damiano Teixeira, Fernanda Fernandez Madeira, Jader de Oliveira, et al.
    American Journal of Tropical Medicine and Hygiene, 2019
    Triatomines are vectors of Trypanosoma cruzi, the etiologic agent of Chagas disease. Although the evolutionary process in triatomine is considered as disruptive, cryptic speciation and homoploid hybridization also are possible modes of speciation. Several analyses suggested Triatoma brasiliensis macromelasoma as a product of hybridization between T. brasiliensis and Triatoma juazeirensis. Thus, we analyzed genetic characteristics (chromosomal analysis, genetic distance for the mitochondrial ND1 gene, and the pattern of bands of internal transcribed spacer [ITS]-1) of these species, with emphasis on the phenomenon of homoploid hybridization. All species showed the same cytogenetic characteristics, low genetic distance for ND1 gene, and the same pattern of ITS-1 bands. We consider that these genetic characteristics, together with the large chromatic polymorphism and the viability of experimental crosses possibly are due to the processes of introgression that these species suffered during the process of homoploid hybridization.
  • New evidence of the evolutionary relationship of the flavida complex with the genus panstrongylus (Hemiptera, triatominae) by Karyosystematic
    K. C. Borsatto, Y. V. Reis, F. F. F. Moreira, M. T. V. Azeredo-Oliveira, K. C. C. Alevi
    Brazilian Journal of Biology, 2018
  • Nucleolar persistence: Peculiar characteristic of spermatogenesis of the vectors of chagas disease (Hemiptera, Triatominae)
    Fernanda Fernandez Madeira, Kelly Cristine Borsatto, Jader de Oliveira, Amanda Ravazi, Maria Tercília Vilela de Azeredo-Oliveira, et al.
    American Journal of Tropical Medicine and Hygiene, 2016
  • Karyosystematics of Triatoma rubrofasciata (De Geer, 1773) (Hemiptera: Reduviidae: Triatominae)
    KAIO CESAR CHABOLI ALEVI, KELLY CRISTINE BORSATTO, FELIPE FERRAZ FIGUEIREDO MOREIRA, JOSÉ JURBERG, MARIA TERCÍLIA VILELA DE AZEREDO-OLIVEIR
    Zootaxa, 2015

RECENT SCHOLAR PUBLICATIONS

  • Omics tools applied to the study of Chagas disease vectors: proteomics and metabolomics
    KC Borsatto, MA Coronado, C Galvão, RK Arni, KCC Alevi
    The American journal of tropical medicine and hygiene 113 (1), 18-24 , 2025
    2025
    Citations: 1
  • HR-MAS NMR metabolomics profile of vero cells under the influence of virus infection and nsP2 inhibitor: a Chikungunya case study
    RS Peinado, LG Martins, CC Pacca, MV Saivish, KC Borsatto, ...
    International journal of molecular sciences 25 (3), 1414 , 2024
    2024
    Citations: 4
  • Análise da interação parasito-vetor da Doença de Chagas-Perfil Metabólico
    KC Borsatto
    Universidade Estadual Paulista (Unesp) , 2024
    2024
  • First evidence of gonadal hybrid dysgenesis in Chagas disease vectors (Hemiptera, Triatominae): Gonad atrophy prevents events of interspecific gene flow and introgression
    LMS Azevedo, NR Cesaretto, J De Oliveira, A Ravazi, YV Dos Reis, ...
    Parasites & Vectors 16 (1), 390 , 2023
    2023
    Citations: 6
  • Morphological, cytological and molecular studies and feeding and defecation pattern of hybrids from experimental crosses between Triatoma sordida and T. rosai (Hemiptera …
    RD Vicente, FF Madeira, KC Borsatto, ACC Garcia, DC Cristal, ...
    Pathogens 11 (11), 1302 , 2022
    2022
    Citations: 7
  • Transcriptomics applied to the study of Chagas disease vectors
    KC Borsatto, MA Coronado, C Galvão, RK Arni, KCC Alevi
    The American journal of tropical medicine and hygiene 106 (4), 1042 , 2022
    2022
    Citations: 5
  • Omics tools applied to the study of Chagas disease vectors: cytogenomics and genomics
    KC Borsatto, MA Coronado, RK Arni, CAK Cesar
    The American Journal of Tropical Medicine and Hygiene 104 (6), 1973 , 2021
    2021
    Citations: 5
  • In silico investigation of Aedes aegypti male-determining factor (NIX): RNA recognition motif-3, structural model and selective nucleic acid binding mode
    MA Coronado, DS Olivier, KC Borsatto, MS Amaral, RK Arni, RJ Eberle
    bioRxiv, 2020.11. 13.381210 , 2020
    2020
    Citations: 1
  • Cytotaxonomy of Dipetalogaster maxima Uhler, 1894 (Hemiptera, Reduviidae, Triatominae)
    KC Borsatto, KCC Alevi, J Oliveira, MTV Azeredo-Oliveira
    Brazilian Journal of Biology 80, 330-335 , 2020
    2020
    Citations: 1
  • CytoKey: identification key for the Chagas disease vectors of the largest Brazilian urban center (São Paulo state), based on cytogenetic data
    KC Borsatto, YV Dos Reis, ACC Garcia, PS De Sousa, ...
    The American Journal of Tropical Medicine and Hygiene 101 (1), 113 , 2019
    2019
    Citations: 15
  • Revisiting the homoploid hybrid speciation process of the Triatoma brasiliensis macromelasoma Galvão, 1956 (Hemiptera, Triatominae) using cytogenetic and molecular markers
    AL Guerra, KC Borsatto, NPD Teixeira, FF Madeira, J de Oliveira, ...
    The American Journal of Tropical Medicine and Hygiene 100 (4), 911 , 2019
    2019
    Citations: 9
  • Identification key for the Chagas disease vectors of five Brazilian states, based on cytogenetic data
    KC Borsatto, MTV Azeredo-Oliveira, KCC Alevi
    The American Journal of Tropical Medicine and Hygiene 100 (2), 303 , 2018
    2018
    Citations: 14
  • New evidence of the evolutionary relationship of the flavida complex with the genus Panstrongylus (Hemiptera, Triatominae) by karyosystematic
    KC Borsatto, YV Reis, FFF Moreira, MTV Azeredo-Oliveira, KCC Alevi
    Brazilian Journal of Biology 78 (04), 802-804 , 2018
    2018
    Citations: 2
  • Nucleolar persistence: peculiar characteristic of spermatogenesis of the vectors of Chagas disease (Hemiptera, triatominae)
    FF Madeira, KC Borsatto, ACC Lima, A Ravazi, J de Oliveira, JA da Rosa, ...
    The American Journal of Tropical Medicine and Hygiene 95 (5), 1118 , 2016
    2016
    Citations: 7
  • Karyosystematics of Triatoma rubrofasciata (De Geer, 1773)(Hemiptera: Reduviidae: Triatominae).
    KC Alevi, KC Borsatto, FF Moreira, J Jurberg, MT De Azeredo-Oliveir
    Zootaxa 3994 (3), 433-438 , 2015
    2015
    Citations: 18

MOST CITED SCHOLAR PUBLICATIONS

  • Karyosystematics of Triatoma rubrofasciata (De Geer, 1773)(Hemiptera: Reduviidae: Triatominae).
    KC Alevi, KC Borsatto, FF Moreira, J Jurberg, MT De Azeredo-Oliveir
    Zootaxa 3994 (3), 433-438 , 2015
    2015
    Citations: 18
  • CytoKey: identification key for the Chagas disease vectors of the largest Brazilian urban center (São Paulo state), based on cytogenetic data
    KC Borsatto, YV Dos Reis, ACC Garcia, PS De Sousa, ...
    The American Journal of Tropical Medicine and Hygiene 101 (1), 113 , 2019
    2019
    Citations: 15
  • Identification key for the Chagas disease vectors of five Brazilian states, based on cytogenetic data
    KC Borsatto, MTV Azeredo-Oliveira, KCC Alevi
    The American Journal of Tropical Medicine and Hygiene 100 (2), 303 , 2018
    2018
    Citations: 14
  • Revisiting the homoploid hybrid speciation process of the Triatoma brasiliensis macromelasoma Galvão, 1956 (Hemiptera, Triatominae) using cytogenetic and molecular markers
    AL Guerra, KC Borsatto, NPD Teixeira, FF Madeira, J de Oliveira, ...
    The American Journal of Tropical Medicine and Hygiene 100 (4), 911 , 2019
    2019
    Citations: 9
  • Morphological, cytological and molecular studies and feeding and defecation pattern of hybrids from experimental crosses between Triatoma sordida and T. rosai (Hemiptera …
    RD Vicente, FF Madeira, KC Borsatto, ACC Garcia, DC Cristal, ...
    Pathogens 11 (11), 1302 , 2022
    2022
    Citations: 7
  • Nucleolar persistence: peculiar characteristic of spermatogenesis of the vectors of Chagas disease (Hemiptera, triatominae)
    FF Madeira, KC Borsatto, ACC Lima, A Ravazi, J de Oliveira, JA da Rosa, ...
    The American Journal of Tropical Medicine and Hygiene 95 (5), 1118 , 2016
    2016
    Citations: 7
  • First evidence of gonadal hybrid dysgenesis in Chagas disease vectors (Hemiptera, Triatominae): Gonad atrophy prevents events of interspecific gene flow and introgression
    LMS Azevedo, NR Cesaretto, J De Oliveira, A Ravazi, YV Dos Reis, ...
    Parasites & Vectors 16 (1), 390 , 2023
    2023
    Citations: 6
  • Transcriptomics applied to the study of Chagas disease vectors
    KC Borsatto, MA Coronado, C Galvão, RK Arni, KCC Alevi
    The American journal of tropical medicine and hygiene 106 (4), 1042 , 2022
    2022
    Citations: 5
  • Omics tools applied to the study of Chagas disease vectors: cytogenomics and genomics
    KC Borsatto, MA Coronado, RK Arni, CAK Cesar
    The American Journal of Tropical Medicine and Hygiene 104 (6), 1973 , 2021
    2021
    Citations: 5
  • HR-MAS NMR metabolomics profile of vero cells under the influence of virus infection and nsP2 inhibitor: a Chikungunya case study
    RS Peinado, LG Martins, CC Pacca, MV Saivish, KC Borsatto, ...
    International journal of molecular sciences 25 (3), 1414 , 2024
    2024
    Citations: 4
  • New evidence of the evolutionary relationship of the flavida complex with the genus Panstrongylus (Hemiptera, Triatominae) by karyosystematic
    KC Borsatto, YV Reis, FFF Moreira, MTV Azeredo-Oliveira, KCC Alevi
    Brazilian Journal of Biology 78 (04), 802-804 , 2018
    2018
    Citations: 2
  • Omics tools applied to the study of Chagas disease vectors: proteomics and metabolomics
    KC Borsatto, MA Coronado, C Galvão, RK Arni, KCC Alevi
    The American journal of tropical medicine and hygiene 113 (1), 18-24 , 2025
    2025
    Citations: 1
  • In silico investigation of Aedes aegypti male-determining factor (NIX): RNA recognition motif-3, structural model and selective nucleic acid binding mode
    MA Coronado, DS Olivier, KC Borsatto, MS Amaral, RK Arni, RJ Eberle
    bioRxiv, 2020.11. 13.381210 , 2020
    2020
    Citations: 1
  • Cytotaxonomy of Dipetalogaster maxima Uhler, 1894 (Hemiptera, Reduviidae, Triatominae)
    KC Borsatto, KCC Alevi, J Oliveira, MTV Azeredo-Oliveira
    Brazilian Journal of Biology 80, 330-335 , 2020
    2020
    Citations: 1
  • Análise da interação parasito-vetor da Doença de Chagas-Perfil Metabólico
    KC Borsatto
    Universidade Estadual Paulista (Unesp) , 2024
    2024