Inorganic Chemistry, General Chemistry, Materials Chemistry, Materials Science
44
Scopus Publications
Scopus Publications
Biofunctional Polyvinyl Alcohol/Xanthan Gum/Gelatin Hydrogel Dressings Loaded with Curcumin: Antibacterial Properties and Cell Viability María José Rivera, Alejandro Cament, Manuel Ahumada, Teresa Corrales, Verónica García, Jesús L. Pablos, Javiera Osorio, Giselle Ramos-González, Leslie Vargas-Saturno, Marcelo Ezquer, J. Andrés Ortiz Gels, 2025 This study explores the development of biocompatible hydrogel dressings incorporating curcumin as an alternative antibacterial agent. In this context, hydrogels were prepared using polyvinyl alcohol, xanthan gum, gelatin, and curcumin as a therapeutic component. FTIR spectroscopy confirmed the successful incorporation of curcumin into the hydrogel matrix, while release profiles demonstrated sustained release. Mechanical testing indicated that xanthan gum reduced elongation and strength in hydrogels, while the combination of xanthan gum and gelatin increased stiffness without loss of elasticity. Curcumin had no major effect on the tensile and rheological properties, preserving the structural integrity of the hydrogels. The hydrogels demonstrated antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus ATCC strains, as well as multidrug methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates. Biocompatibility was confirmed through viability assays with immortalized human keratinocytes (HaCaT) and adult human dermal fibroblasts (HDFa), showing no acute cytotoxic effects after 48 h of exposure. Their effective action against clinically relevant bacteria and high cytocompatibility position these hydrogels as promising candidates for infection management and antibiotic resistance mitigation in wound care applications.
Anti-inflammatory and antibacterial hydrogel based on a polymerizable ionic liquid J.A. Romero-Antolín, N. Gómez-Cerezo, M. Manzano, J.L. Pablos, M. Vallet-Regí Acta Biomaterialia, 2025 In the present era, the treatment of skin-infected wounds and their associated inflammation constitutes a significant challenge. These infections have the potential to impede the healing process and become a life-threatening pathology, particularly due to the rise of bacterial resistance. Hydrogels could successfully address this issue due to their unique capabilities and versatility. Among them, natural polymer-based hydrogels are especially advantageous as they resemble the extracellular matrix (ECM) and mechanical properties of natural tissues. In this study, we propose a dual-action hydrogel composed of methacrylated gelatin as a matrix and a salicylate (Sal) anion-exchanged polymerizable ionic liquid (PIL) to achieve anti-inflammatory and antibacterial activities. This material facilitated cell attachment and colonization with mouse endothelial fibroblasts. A flow cytometry assay was conducted to evaluate the anti-inflammatory effect, and demonstrated the differentiation of mouse macrophages to an M2 (reparative) phenotype. Therefore, the levels of TNF-α, interleukin-6 (IL-6), and interleukin (IL-10) were quantified to further evaluate this effect, demonstrating an inhibition on the pro-inflammatory ones. The inherent antibacterial capacity of the PIL was demonstrated against Staphylococcus aureus and Escherichia coli, thereby corroborating its potential as a wound dressing. To the best of our knowledge, this is the first reported hydrogel incorporating an anion-exchanged polymerizable ionic liquid that is capable of promoting macrophage differentiation into a reparative phenotype, of reducing pro-inflammatory cytokines, and of simultaneously retaining antibacterial activity. These features open the gate to the potential application of this hydrogel as a wound dressing. STATEMENT OF SIGNIFICANCE: Bacterial wound infections may lead to severe problems due to their associate tissue inflammation and the emergence of bacterial resistance. In this sense, local therapies such as hydrogels have gathered much attention as alternative therapies for these pathologies. In this work, we have developed a natural polymer-based hydrogel copolymerized with a polymerizable ionic liquid containing salicylate as an anion. The hydrogel was shown to be biocompatible, and promoted macrophage differentiation to a reparative phenotype, while reducing the levels of pro-inflammatory cytokines. Finally, the high antibacterial capability against both gram-positive and gram-negative bacteria makes it a promising candidate for use in wound dressings.
Regenerative medicine: Hydrogels and mesoporous silica nanoparticles Jesús L. Pablos, Daniel Lozano, Miguel Manzano, María Vallet-Regí Materials Today Bio, 2024 Hydrogels, that are crosslinked polymer networks, can absorb huge quantities of water and/or biological fluids. Their physical properties, such as elasticity and soft tissue, together with their biocompatibility and biodegradability, closely resemble living tissues. The versatility of hydrogels has fuelled their application in various fields, such as agriculture, biomaterials, the food industry, drug delivery, tissue engineering, and regenerative medicine. Their combination with nanoparticles, specifically with Mesoporous Silica Nanoparticles (MSNs), have elevated these composites to the next level, since MSNs could improve the hydrogel mechanical properties, their ability to encapsulate and controlled release great amounts of different therapeutic agents, and their responsiveness to a variety of external and internal stimuli. In this review, the main features of both MSNs and hydrogels are introduced, followed by the discussion of different hydrogels-MSNs structures and an overview of their use in different applications, such as drug delivery technologies and tissue engineering. • Hydrogels are flexible, soft, biodegradable, and biocompatible, making them ideal for tissue engineering and regenerative medicine. • Clinical use of hydrogels is often restricted by poor mechanical properties and limited control over biodegradation and drug release. • Combining hydrogels with nanoparticles, especially Mesoporous Silica Nanoparticles (MSNs), presents a promising alternative. • This combination enhances mechanical properties, improves drug encapsulation and release, and increases responsiveness to stimuli. • Hybrid hydrogels enable localized therapy with improved biocompatibility and protection for nanocarriers.
Enhancing Osteoblastic Cell Cultures with Gelatin Methacryloyl, Bovine Lactoferrin, and Bioactive Mesoporous Glass Scaffolds Loaded with Distinct Parsley Extracts Laura Isabel Arias-Rodríguez, Jesús L. Pablos, María Vallet-Regí, Martha A. Rodríguez-Mendiola, Carlos Arias-Castro, Sandra Sánchez-Salcedo, Antonio J. Salinas Biomolecules, 2023 The increasing interest in innovative solutions for addressing bone defects has driven research into the use of Bioactive Mesoporous Glasses (MBGs). These materials, distinguished by their well-ordered mesoporous structure, possess the capability to accommodate plant extracts with well-established osteogenic properties, including bovine lactoferrin (bLF), as part of their 3D scaffold composition. This harmonizes seamlessly with the ongoing advancements in the field of biomedicine. In this study, we fabricated 3D scaffolds utilizing MBGs loaded with extracts from parsley leaves (PL) and embryogenic cultures (EC), rich in bioactive compounds such as apigenin and kaempferol, which hold potential benefits for bone metabolism. Gelatin Methacryloyl (GelMa) served as the polymer, and bLF was included in the formulation. Cytocompatibility, Runx2 gene expression, ALP enzyme activity, and biomineralization were assessed in preosteoblastic MC3T3-E1 cell cultures. MBGs effectively integrated PL and EC extracts with loadings between 22.6 ± 0.1 and 43.6 ± 0.3 µM for PL and 26.3 ± 0.3 and 46.8 ± 0.4 µM for EC, ensuring cell viability through a release percentage between 28.3% and 59.9%. The incorporation of bLF in the 3D scaffold formulation showed significant differences compared to the control in all assays, even at concentrations below 0.2 µM. Combinations, especially PL + bLF at 0.19 µM, demonstrated additive potential, with superior biomineralization compared to EC. In summary, this study highlights the effectiveness of MBGs in incorporating PL and EC extracts, along with bLF, into 3D scaffolds. The results underscore cytocompatibility, osteogenic activity, and biomineralization, offering exciting potential for future in vivo applications.
New Photocrosslinked 3D Foamed Scaffolds Based on GelMA Copolymers: Potential Application in Bone Tissue Engineering Jesús L. Pablos, Javier Jiménez-Holguín, Sandra Sánchez Salcedo, Antonio J. Salinas, Teresa Corrales, María Vallet-Regí Gels, 2023 The production of customized polymeric hydrogels in the form of 3D scaffolds with application in bone tissue engineering is currently a topic of great interest. Based on gelatin methacryloyl (GelMa) as one of the most popular used biomaterials, GelMa with two different methacryloylation degrees (DM) was obtained, to achieve crosslinked polymer networks by photoinitiated radical polymerization. In this work, we present the obtention of new 3D foamed scaffolds based on ternary copolymers of GelMa with vinylpyrrolidone (VP) and 2-hydroxyethylmethacrylate (HEMA). All biopolymers obtained in this work were characterized by infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), whose results confirm the presence of all copolymers in the crosslinked biomaterial. In addition, scanning electron microscopy (SEM) pictures were obtained verifying the presence of the porosity created by freeze-drying process. In addition, the variation in its swelling degree and its enzymatic degradation in vitro was analyzed as a function of the different copolymers obtained. This has allowed us to observe good control of the variation in these properties described above in a simple way by varying the composition of the different comonomers used. Finally, with these concepts in mind, biopolymers obtained were tested through assessment of several biological parameters such as cell viability and differentiation with MC3T3-E1 pre-osteoblastic cell line. Results obtained show that these biopolymers maintain good results in terms of cell viability and differentiation, along with tunable properties in terms of hydrophilic character, mechanical properties and enzymatic degradation.
Reversible Colorimetric and Fluorescence Solid Sensors Based on Aryl Hydrazone Derivatives of 1,8-Naphthalimides for Caustic Media and Biogenic Amine Vapors Jesús L. Pablos, Sabela Fernández-Alonso, Fernando Catalina, Teresa Corrales Chemosensors, 2022 Fluorescence and colorimetric solid sensors for caustic media and biogenic amine vapors have been prepared. For this purpose, several hydrazone derivatives of naphthalimides were synthesized and anchored to a photo-crosslinked membrane functionalized with acid chloride groups. The membranes were characterized using different techniques, and their thermal properties and swelling degree were determined. The new naphthalimides and the membranes were evaluated as sensors by determining the change in their spectroscopic properties of absorption and fluorescence with pH. The polymeric sensors exhibit improved stability and can be reused, as a consequence of their solid character and the reversibility of the process. Furthermore, membranes were evaluated as a sensor of trimethylamine vapors through their absorption and fluorescence bands, and the color change in the membrane showed that it could be used to detect basic media with the naked eye. Finally, membranes were packaged in Petri dishes at a controlled temperature with fresh fish bought in the local market. Then, the real chance of using the sensory materials was determined by analyzing the color change in samples.
New PCL/PEC Blends: In Vitro Cell Response of Preosteoblasts and Human Mesenchymal Stem Cells Jesus L. Pablos, Mónica Cicuéndez, María Hernández-Rivas, Fernando Catalina, María Vallet-Regí, Teresa Corrales Biology, 2022 In this study, new blends of PCL/PEC have been prepared in an easy manner by casting with the objective of obtaining new biomaterials to apply to tissue engineering and bone regeneration. The PCL/PEC blends obtained, together with neat polymer blends, were characterized by infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). This full characterization is the key to disentangle the miscibility, which means good compatibility, of the polymer blends used in this work. The addition of increasing amounts of PEC, has shown in the new biomaterials obtained, a remarkable improvement in relation with the mechanical properties (manageable materials) and above all, in terms of an increase in their hydrophilic character with respect to the PCL neat polymer. The improvement of all these properties is reflected in their biological properties. With these thoughts in mind, the blends obtained were tested through the assessment of several biological parameters such as cell viability, proliferation, and differentiation of both the MC3T3-E1 osteoblastic cell line and hMSCs to evaluate their cell response to different polymer membranes aimed at bone tissue regeneration. “In vitro” biocompatibility methods have been chosen rather than in vivo studies due to their lower cost, faster procedure time, and minimum ethical concerns, and because it was the first time that the biological effects of these blends were studied. The results show that the PCL/PEC blends obtained, with tunable properties in terms of hydrophilic character and hydrolytic degradation, may be regarded as good candidates to perform “in vivo” tests and check their real-life applicability for bone regeneration. The polymer acronym (the weight percentage in the sub index) is PCLx/PECy as noted in table one with the summary of compositions.
Porous aromatic polyamides the easy and green way Blanca S. Pascual, Miriam Trigo-López, José A. Reglero Ruiz, Jesús L. Pablos, Juan C. Bertolín, César Represa, José V. Cuevas, Félix C. García, José M. García European Polymer Journal, 2019
