Veterinary, Equine, Physiology (medical), Animal Science and Zoology
18
Scopus Publications
774
Scholar Citations
15
Scholar h-index
22
Scholar i10-index
Scopus Publications
Biocompatibility and Bactericidal Action of Alginate/Tofu-Based Hydrogels Loaded with Ozonated Sunflower Oil Cristiane Marissa Piacitelli Prado Ferreira, Jair Camargo Ferreira, Heber Eduardo Andrada, Maria Anita Lemos Vasconcelos Ambrósio, Julia Mirian Paulino, Manuela Pugliesi Assis Lima, Raquel Alves dos Santos, Eduardo Ferreira Molina Ozone Science and Engineering, 2026 The study aimed to characterize the biocompatibility and antimicrobial action of sodium alginate (SA)/tofu-basedhydrogels enriched with ozonized sunflower oil. Three formulations were developed loading the hydrogel with ozonized oil at weight ratios of 3:1, 2:1 and 1:1 (O3-1, O3-2 and O3-3 SA/tofu, respectively). The physicochemical characterization of the hydrogels was done by Fourier-transform infrared spectroscopy (FTIR). Antimicrobial susceptibility testing (AST), minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were used to determine their antimicrobial potential against skin bacteria (Staphylococcus aureus, S. epidermidis, Streptococcus pyogenes and Escherichia coli). The biocompatibility of the formulations with fibroblast (GM07492-A) was characterized by XTT and wound healing assays. The identification of a peak at 1000 cm−1 in the spectrum of the ozonized hydrogels confirmed the successful oil-loading. Non-ozonized hydrogel did not show antimicrobial action (MIC and MBC > 100%), while the three ozonized formulations inactivated the four bacteria (MBC and MIC ranged from 0.39 to 50.0%). The viability of fibroblast cells remained unchanged (p > 0.1) after treatment with O3-1, O3-2 or O3-3 SA/tofu. Similar to non-ozonized formulation (p < 0.001), complete wound closure was detected 48 h after treatment with SA/tofu O3-1. The ozonized hydrogels were biocompatible with fibroblasts and showed germicidal action against skin bacteria.
Ozonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditions Barbara Higa, Bianca Souza Cintra, Carmen Magaly Álvarez, Arthur Barcelos Ribeiro, Jair Camargo Ferreira, Denise Crispim Tavares, Vanessa Enriquez, Luis R Martinez, Regina Helena Pires Medical Mycology, 2022 This study explores the growth of bacterial, fungal, and interkingdom biofilms under aerobiosis or microaerobic conditions and the effect of ozonated sunflower oil on these biofilms. Candida species and Streptococcus mutans were used to study this interaction due to their importance in oral health and disease as these microorganisms display a synergistic relationship that manifests in the onset of caries and tooth decay. Biofilms were developed in a 96-well microtiter plate at 37ºC for 24 h, under aerobiosis or microaerobic conditions, and treated with ozonated oil for 5 to 120 min. All the microorganisms formed biofilms in both oxygenation conditions. Scanning electron microscopy was used to visualize biofilm morphology. Rodent experiments were performed to verify the oil-related toxicity and its efficacy in oral candidiasis. The growth of all Candida species was increased when co-cultured with S. mutans, whilst the growth of bacterium was greater only when co-cultured with C. krusei and C. orthopsilosis under aerobiosis and microaerobic conditions, respectively. Regardless of the oxygenation condition, ozonated oil significantly reduced the viability of all the tested biofilms and infected mice, showing remarkable microbicidal activity as corroborated with confocal microscopy and minimal toxicity. Thus, ozonated oil therapy can be explored as a strategy to control diseases associated with these biofilms especially in the oral cavity. Lay Summary We demonstrated that ozonated sunflower oil is effective at killing the biofilms formed by Candida species, by the bacterium Streptococcus mutans, or by both micoorganisms that can interact in the oral cavity, making it a potential therapeutic option for the treatment of these infections.
Ozone Gas and Ozonized Sunflower Oil as Alternative Therapies against Pythium Insidiosum Isolated from Dogs Bruna N. Carrijo, Regina H. Pires, Guilherme B. Costa, Francine G. Guiotto, Viviani S. Rodrigues, Jair C. Ferreira Ozone Science and Engineering, 2022 Conventional antimicrobial therapies are less effective against pythiosis. Therefore, the goal of this study was to characterize the effects of two ozone (O3) presentations on the growth of canine Pythium insidiosum. In Experiment 1, the pathogen was treated with O3 gas at different concentrations (32, 52, or 72 μg O3 mL−1 for 30 min) or exposure times (72 μg O3 mL−1 for 5, 15, or 30 min). In Experiment 2, pathogen samples were covered with ozonized sunflower oil with distinct peroxide indices. The growth area of P. insidiosum was measured during the first 14 days after treatment. Samples without growth were recultured to assess the germicidal or germistatic action of the respective therapy. In Experiment 1, treatment for 30 min had germicidal action regardless of the gas concentration, whereas shorter exposures to O3 were not effective. In Experiment 2, only ozonized sunflower oil with a peroxide index > 600 mmol equiv. kg−1 inactivated the pathogen. The findings of the present study suggest that exposure to O3 gas and ozonized sunflower oil with a high peroxide index are promising options for the long-lasting treatment of cutaneous pythiosis in humans and domestic animals.
Endometrial angiogenesis induced by uterine insufflation with an oxygen–ozone gas mixture in mares Jair Camargo Ferreira, Rafael Silva Amaral, Letícia Mara Cardoso, Sophia Panelli Marchió, Marcela Aldrovani Rodrigues, Carlos Eduardo Fonseca Alves, Cezinande de Meira Reproduction in Domestic Animals, 2021 Ozone (O3) therapy has been used to improve peripheral tissue oxygenation in humans and domestic animals. The goal of the present study was to characterize histological changes in the endometria of healthy equines following tissue exposure to gas mixtures enriched with different concentrations of O3. Cycling mares without endometrial degeneration were divided into three groups according to treatment (n = 9 mares/group). The uteri from the O3, ½O3 and control groups were insufflated for 3 min with gas containing 42, 21 and 0 μg O3 ml‐1, respectively. Treatments were performed every three days from D0 to D6. Endometrial samples were collected immediately before the first treatment and 24 hr after the last treatment. The following nine histological parameters were evaluated: (i) the number of endometrial blood vessels, (ii) endometrial vascular degree (EVD), (iii) increase rate of blood vessels, (iv) increase rate of EVD, (v) glandular total area, (vi) glandular lumen area, (vii) intraglandular secretion area, (viii) glandular epithelial height and (ix) luminal epithelial height. In the O3 group, a positive effect from treatment (p < .01) was detected for all vascular parameters (i, ii, iii and iv), glandular total area, intraglandular secretion area and glandular epithelial height. Compared to the control group, the ½O3 group had greater (p < .01) EVD (84.1 ± 12%) and a higher increase rate of blood vessels (151.9 ± 47.1%). Uterine insufflation with low or intermediate concentrations of the O2–O3 gas mixture induced endometrial angiogenesis. Morphometry, but not morphology, of the endometrial glands was affected by local O3 therapy. These findings would be of great significance for the development of new therapies for infertility in mares.
Luteal vascularity and embryo dynamics in mares during early gestation: Effect of age and endometrial degeneration Jair Camargo Ferreira, Yatta Linhares Boakari, Noeme Sousa Rocha, Fernanda Saules Ignácio, Guilherme Barbosa da Costa, Cezinande de Meira Reproduction in Domestic Animals, 2019 ContentsThe present study characterized the luteal status and the dynamic of the conceptus during the first 20 days of gestation in mares with different ages and degrees of endometrial degeneration. Total area of the corpus luteum (CL), luteal vascularity, CL area with blood signals, progesterone concentrations (P4), embryonic vesicle diameter, number of embryonic location changes, embryonic fixation position and uterine contractility were evaluated. In Experiment 1, mares ≤6 years of age (Young group, 5.6 ± 0.2 years, n = 7 mares) and mares ≥15 years of age (Old group, 17.2 ± 0.9 years, n = 6 mares) were used to investigate the effect of age. In Experiment 2, the luteal and embryonic parameters were compared between mares with minimal (Mild group, endometrial category I, n = 9 mares) and severe (Severe group, endometrial category III, n = 7 mares) endometrial degeneration. The Old and Severe groups had greater (p ≤ 0.04) total CL area and reduced luteal vascularity (p ≤ 0.04) than the Young and Mild groups, respectively. However, P4 levels and CL area with blood signals were similar (p ≥ 0.8) between the groups. A negative effect of age (p < 0.01), but not of endometrial degeneration (p = 0.6), was found for the embryonic vesicle diameter. The conceptus mobility was high (p > 0.1) until day 14 of gestation in the Severe group, while a reduced number of changes of the embryo location was detected earlier (p < 0.05) in the Old group. In conclusion, the newly formed CL of aged mares and mares with severe endometrial degeneration suffered a structural remodelling to safeguard the local blood supply and the continuous P4 output during early gestation. Moreover, an earlier reduction of the embryonic mobility and a delayed development of the conceptus were associated with advanced age, regardless of the degree of endometrial degeneration.
Comparative analysis of four commercial on-farm culture methods to identify bacteria associated with clinical mastitis in dairy cattle Jair C. Ferreira, Marilia S. Gomes, Erika C. R. Bonsaglia, Igor F. Canisso, Edgar F. Garrett, Jamie L. Stewart, Ziyao Zhou, Fabio S. Lima Plos One, 2018 Several multiple-media culture systems have become commercially available for on-farm identification of mastitis-associated pathogens. However, the accuracy of these systems has not been thoroughly and independently validated against microbiological evaluations performed by referral laboratories. Therefore, the purpose of the present study was to evaluate the performance of commercially available culture plates (Accumast, Minnesota Easy System, SSGN and SSGNC Quad plates) to identify pathogens associated with clinical mastitis in dairy cows. Milk samples from the affected quarter with clinical mastitis were aerobically cultured with the on-farm culture systems and by two additional reference laboratories. Agreeing results from both standard laboratories were denoted as the reference standard (RS). Accuracy (Ac), sensitivity (Se), specificity (Sp), positive and negative predictive values (PPV and NPV, respectively) and Cohen's kappa coefficient (k) of on-farm plates were determined based on the RS culture of 211 milk samples. All four plate-systems correctly identified ≥ 84.9% of milk samples with no bacterial growth. Accumast had greater values for all overall predictive factors (Ac, Se, Sp, PPV and NPV) and a substantial agreement (k = 0.79) with RS. The inter-rater agreements of Minnesota, SSGN, and SSGNC with RS were moderate (0.45 ≤ k ≤ 0.55). The effectiveness to categorize bacterial colonies at the genus and species was numerically different amongst the commercial plates. Our findings suggest that Accumast was the most accurate on-farm culture system for identification of mastitis-associated pathogens of the four systems included in the analysis.
Endometrial gland remodeling and preserved luminal ultrastructure in postpartum cows infused with ozonated sunflower oil RMA Ferreira JC, Oliveira MS, Ferreira CMPP, Almeida MB, Rodrigues HA ... Animal Reoridduction 23 (2), e20260001 , 2026 2026
From oxidative signaling to clinical practice: The role of ozone therapy in skin health CMPP Ferreira, APB Neves, JC Ferreira Pathways to Health: Innovations and Approaches in Health Sciences 1, 19 , 2026 2026
Antimicrobial activity of ozone, low power laser and laser-ozone association against cultures of oral bacteria from captive big cats JC Ferreira, YC Sousa, RA Barros, C Ferreira, A MAV, SR Ambrósio, ... Expanded Science: Innovation and Research 1, 19 , 2025 2025
Biocompatibility and Bactericidal Action of Alginate/Tofu-Based Hydrogels Loaded with Ozonated Sunflower Oil C Marissa Piacitelli Prado Ferreira, J Camargo Ferreira, ... Ozone: Science & Engineering, 1-13 , 2025 2025
Ozonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditions B Higa, BS Cintra, CM Álvarez, AB Ribeiro, JC Ferreira, DC Tavares, ... Medical mycology 60 (8), myac055 , 2022 2022 Citations: 19
Ozonioterapia no tratamento auxiliar de éguas inférteis: Potencial angiogênico e antimicrobiano JC Ferreira, RS Amaral, B de Souza Cintra Rev Bras Reprod Anim 45 (4), 482-488 , 2021 2021 Citations: 2
Endometrial angiogenesis induced by uterine insufflation with an oxygen‐ozone gas mixture in mares J Camargo Ferreira, R Silva Amaral, L Mara Cardoso, S Panelli Marchió, ... Reproduction in Domestic Animals, 1-8 , 2021 2021 Citations: 13
The in vitro effect of ozone therapy against equine Pythium insidiosum JC Ferreira, RH Pires, GB da Costa, BN Carrijo, FG Guiotto, VS Rodrigues Journal of Equine Veterinary Science 98, 103305 , 2021 2021 Citations: 18
Ozone therapy in the supporting treatment of infertile mares: angiogenic and antimicrobial actions. JC Ferreira, RS Amaral, BS Cintra 2021 Citations: 1
Ozone Gas and Ozonized Sunflower Oil as Alternative Therapies against Pythium Insidiosum Isolated from Dogs JCF Carrijo, Bruna N., Regina H. Pires, Guilherme B. Costa, Francine G ... Ozone: Science & Engineering, 1-9 , 2021 2021 Citations: 10
Heat stress does not affect induced luteolysis in Holstein cows HDG Mogollón, RA Ferrazza, VH Vallejo, FC Destro, JC Ochoa, ... Journal of dairy science 103 (6), 5629-5633 , 2020 2020 Citations: 4
Luteal vascularity and embryo dynamics in mares during early gestation: Effect of age and endometrial degeneration J Camargo Ferreira, Y Linhares Boakari, N Sousa Rocha, ... Reproduction in domestic animals 54 (3), 571-579 , 2019 2019 Citations: 10
Developmental aspects of early pregnancy in mares C Meira, JC Ferreira, ESM Silva, FS Ignácio Animal Reproduction (AR) 9 (3), 166-172 , 2018 2018 Citations: 12
Comparative analysis of four commercial on-farm culture methods to identify bacteria associated with clinical mastitis in dairy cattle JC Ferreira, MS Gomes, ECR Bonsaglia, IF Canisso, EF Garrett, ... PLoS One 13 (3), e0194211 , 2018 2018 Citations: 61
Hemodynamics of the corpus luteum in mares during experimentally impaired luteogenesis and partial luteolysis JC Ferreira, LF Novaes Filho, YL Boakari, HS Canesin, DL Thompson Jr, ... Theriogenology 107, 78-84 , 2018 2018 Citations: 18
Influence of two ovulation-inducing agents on the pituitary response and follicle blood flow in mares YL Boakari, JC Ferreira, HS Canesin, DL Thompson Jr, FS Lima, ... Theriogenology 100, 95-99 , 2017 2017 Citations: 15
Cloprostenol administration in the first week postpartum reduces expression of oxytocin receptors in the endometrium in Holstein-Zebu cows A Camargos, S Wohlres-Viana, IF Costa, LS Camargo, JC Ferreira, ... Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69, 821-829 , 2017 2017 Citations: 1
Effect of age and endometrial degenerative changes on uterine blood flow during early gestation in mares JC Ferreira, HS Canesin, FS Ignácio, NS Rocha, CR Pinto, C Meira Theriogenology 84 (7), 1123-1130 , 2015 2015 Citations: 33
Real-time characterization of the uterine blood flow in mares before and after artificial insemination JC Ferreira, FS Ignácio, NS Rocha, DL Thompson Jr, CR Pinto, C Meira Animal Reproduction Science 160, 90-96 , 2015 2015 Citations: 15
Characterization of luteal blood flow and secretion of progesterone in mares treated with human chorionic gonadotropin for ovulation induction or during early diestrus RM Romano, JC Ferreira, H de Siqueira Canesin, YL Boakari, FS Ignácio, ... Journal of Equine Veterinary Science 35 (7), 591-597 , 2015 2015 Citations: 13
MOST CITED SCHOLAR PUBLICATIONS
Role of follicular estradiol-17beta in timing of luteolysis in heifers RR Araujo, OJ Ginther, JC Ferreira, MM Palhão, MA Beg, MC Wiltbank Biology of Reproduction 81 (2), 426-437 , 2009 2009 Citations: 151
Uterine blood flow and perfusion in mares with uterine cysts: effect of the size of the cystic area and age JC Ferreira, EL Gastal, OJ Ginther Reproduction 135 (4), 541-550 , 2008 2008 Citations: 81
Characterisation of pulses of 13, 14-dihydro-15-keto-PGF2alpha (PGFM) and relationships between PGFM pulses and luteal blood flow before, during, and after luteolysis in mares OJ Ginther, BL Rodrigues, JC Ferreira, RR Araujo, MA Beg Reproduction, Fertility and Development 20 (6), 684-693 , 2008 2008 Citations: 75
Comparative analysis of four commercial on-farm culture methods to identify bacteria associated with clinical mastitis in dairy cattle JC Ferreira, MS Gomes, ECR Bonsaglia, IF Canisso, EF Garrett, ... PLoS One 13 (3), e0194211 , 2018 2018 Citations: 61
Temporal relationships of the LH surge and ovulation to echotexture and power Doppler signals of blood flow in the wall of the preovulatory follicle in heifers MAR Siddiqui, JC Ferreira, EL Gastal, MA Beg, DA Cooper, OJ Ginther Reproduction, Fertility and Development 22 (7), 1110-1117 , 2010 2010 Citations: 48
Adequação dos encaminhamentos de gestações de alto-risco na rede básica de atenção à saúde de Porto Alegre, Rio Grande do Sul, Brasil JA Buchabqui, E Capp, J Ferreira Revista Brasileira de Saúde Materno Infantil 6 (1), 23-29 , 2006 2006 Citations: 37
Effect of age and endometrial degenerative changes on uterine blood flow during early gestation in mares JC Ferreira, HS Canesin, FS Ignácio, NS Rocha, CR Pinto, C Meira Theriogenology 84 (7), 1123-1130 , 2015 2015 Citations: 33
Doppler ultrasonography principles and methods of evaluation of the reproductive tract in mares JC Ferreira, FS Ignácio, C de Meira Acta Scientiae Veterinariae 39 (1), s105-s111 , 2011 2011 Citations: 29
Luteal changes after treatment with sub-luteolytic doses of prostaglandin (cloprostenol sodium) in cattle E Trevisol, JC Ferreira, CL Ackermann, FC Destro, WC Marques Filho, ... Animal reproduction science 153, 8-12 , 2015 2015 Citations: 27
Aplicação da ultrassonografia colorida doppler em programas de transferência de embriões equinos JC Ferreira, C Meira Ciência Rural 41 (6), 1063-1069 , 2011 2011 Citations: 20
Ozonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditions B Higa, BS Cintra, CM Álvarez, AB Ribeiro, JC Ferreira, DC Tavares, ... Medical mycology 60 (8), myac055 , 2022 2022 Citations: 19
The in vitro effect of ozone therapy against equine Pythium insidiosum JC Ferreira, RH Pires, GB da Costa, BN Carrijo, FG Guiotto, VS Rodrigues Journal of Equine Veterinary Science 98, 103305 , 2021 2021 Citations: 18
Hemodynamics of the corpus luteum in mares during experimentally impaired luteogenesis and partial luteolysis JC Ferreira, LF Novaes Filho, YL Boakari, HS Canesin, DL Thompson Jr, ... Theriogenology 107, 78-84 , 2018 2018 Citations: 18
Uterine vascular perfusion and spectral-Doppler measurements during early gestation in mares: new concepts of evaluation JC Ferreira, FS Ignácio, C Meira 2010 Citations: 16
Influence of two ovulation-inducing agents on the pituitary response and follicle blood flow in mares YL Boakari, JC Ferreira, HS Canesin, DL Thompson Jr, FS Lima, ... Theriogenology 100, 95-99 , 2017 2017 Citations: 15
Real-time characterization of the uterine blood flow in mares before and after artificial insemination JC Ferreira, FS Ignácio, NS Rocha, DL Thompson Jr, CR Pinto, C Meira Animal Reproduction Science 160, 90-96 , 2015 2015 Citations: 15
Uso de antitoxina tetânica por via intratecal e endovenosa no tratamento de tétano acidental em equino: Relato de caso AA Silva, UJP Stelmann, JP Papa, EF Fonseca, FS Ignácio, JC Ferreira, ... Revista Científica Eletrônica de Medicina Veterinária 14, 1-11 , 2010 2010 Citations: 14
Endometrial angiogenesis induced by uterine insufflation with an oxygen‐ozone gas mixture in mares J Camargo Ferreira, R Silva Amaral, L Mara Cardoso, S Panelli Marchió, ... Reproduction in Domestic Animals, 1-8 , 2021 2021 Citations: 13
Characterization of luteal blood flow and secretion of progesterone in mares treated with human chorionic gonadotropin for ovulation induction or during early diestrus RM Romano, JC Ferreira, H de Siqueira Canesin, YL Boakari, FS Ignácio, ... Journal of Equine Veterinary Science 35 (7), 591-597 , 2015 2015 Citations: 13
Developmental aspects of early pregnancy in mares C Meira, JC Ferreira, ESM Silva, FS Ignácio Animal Reproduction (AR) 9 (3), 166-172 , 2018 2018 Citations: 12