Department of Engineering for Innovation Medicine, Section of Innovation Biomedicine, Hematology Area, University of Verona Azienda Ospedaliera Universitaria Integrata di Verona
Serositis as possible manifestation in MDS/AML patients with complex karyotype and TP53 mutation: case series Eleonora Gugole, Edoardo Tamellini, Sara Jance, Nicola Serafin, Pietro Antonini, Chiara Colato, Mauro Krampera, Isacco Ferrarini, Ilaria Tanasi Annals of Hematology, 2026 Myelodysplastic syndromes (MDS) and Acute Myeloid Leukemia (AML) are increasingly recognized to exhibit immune dysregulation, which can occasionally present with autoinflammatory manifestations. We describe four patients with MDS/AML harboring p53 mutations and a complex karyotype who developed inflammatory serositis without infectious correlation. All patients showed clinical improvement following corticosteroid therapy.
Biconcave vertebral fractures as a possible distinctive feature of indolent systemic mastocytosis compared with primary osteoporosis Gaetano Paride Arcidiacono, Marco Onofrio Torres, Francesca Zanchetta, Sofia Testoni, Giovanni Tripepi, Alberta Cecchinato, Martin Diogo, Mor Peleg Falb, Francesca Guidolin, Irene Bertozzi, Marco Pizzi, Mauro Cancian, Riccardo Senter, Ilaria Gianesello, Francesca Caroppo, Ilaria Tanasi, Roberta Zanotti, Lorenza Belletti, Maria Fusaro, Elena Campello, Stefania Sella, Paolo Simioni, Sandro Giannini Osteoporosis International, 2026 Indolent systemic mastocytosis (ISM) is a rare cause of skeletal fragility. In this study, patients with ISM showed a markedly higher prevalence of biconcave vertebral fractures compared with primary osteoporosis. Assessing fracture morphology may aid in differentiating ISM, reducing diagnostic delay, and guiding appropriate management. PURPOSE: Indolent systemic mastocytosis (ISM) is a rare disorder frequently associated with bone fragility and vertebral fractures (VFx). While the morphology of VFx has been studied in other metabolic bone diseases (e.g., osteomalacia), its role in ISM remains unclear. We aimed to evaluate the prevalence of specific VFx morphologies in ISM and assess their potential diagnostic value compared with primary osteoporosis (OP). METHODS: We retrospectively enrolled adults with ISM and VFx, diagnosed according to WHO and ICC criteria. To increase statistical power, each ISM case was compared with two controls with OP. Clinical, densitometric, and laboratory data were collected. VFx were assessed on lateral spine X-rays (T4-L4) using Genant's criteria and categorized as wedge or biconcave. RESULTS: Sixty-six patients were included: 22 with ISM and 44 with OP. ISM patients were younger (68 ± 8 vs. 77 ± 7 years, p < 0.001) and more frequently male (32% vs. 7%, p = 0.043). ISM patients had more VFx (median 4 vs. 3, p = 0.039) and a higher proportion of biconcave VFx (80% vs. 22%, p < 0.001). ROC analysis showed high discriminatory power for the number and proportion of biconcave VFx (AUC 0.878 and AUC 0.919, respectively) in distinguishing ISM from OP, with optimal cut-offs of > 2 biconcave VFx or > 50% of total VFx. These findings remained consistent after adjustment for age and sex. CONCLUSION: Multiple biconcave VFx are significantly associated with ISM and may help differentiate it from primary OP. Assessment of fracture morphology could reduce diagnostic delay and guide appropriate management in patients with vertebral fragility fractures.
Real-world multicenter analysis of CPX-351 efficacy in patients aged less than 60 years with secondary acute myeloid leukemia Calogero Vetro, Bruno Garibaldi, Francesco Grimaldi, Patrizia Chiusolo, Carla Filì, Carola Riva, Valeria Cardinali, Marinunzia Franciosa, Federica Pilo, Raffaele Palmieri, Lorenzo Esposito, Diego Menotti, Salvatore Perrone, Antonino Mulè, Caterina Alati, Ernesto Vigna, Michela Dargenio, De De Luca, Alessandra Sperotto, Ilaria Tanasi, Cristina Papayannidis, Matteo Molica, Paola Minetto, Giorgia Battipaglia, Mara Memoli, Prassede Salutari, Lorenzo Brunetti, Elisabetta Todisco, Ivana Lotesoriere, Alessandro Isidori, Giuseppe Alberto Palumbo, Mario Annunziata, Michele Gottardi, Massimo Gentile, Maria Paola Martelli, Debora Capelli, Massimo Martino, Marco Rossi, Felicetto Ferrara, Adriano Venditti, Claudio Romani, Sara Galimberti, Anna Candoni, Luana Fianchi, Livio Pagano, Giovanni Marconi, Roberto Massimo Lemoli, Francesco Di Raimondo, Atto Billio, Fanny Erika Palumbo, Fabrizio Pane, Andrea Duminuco, Fabio Guolo Blood Cancer Journal, 2025 Initial evidence of CPX-351 activity in patients younger than 60 years emerged from a phase I trial [ 1 ]; however, subsequent pivotal phase III trial focused on older patients (≥60 years), where CPX-351 demonstrated superiority over conventional ‘7 + 3’ induction, in in secondary acute myeloid leukemia (sAML), including therapy-related AML (t-AML) and AML with myelodysplasia-related changes (AML-MRC), showing higher overall response rates (ORR), improved overall survival (OS) and increased haematopoetic stem cell transplantation (HSCT) rate [ 2 ].
Correction: Real-world multicenter analysis of CPX-351 efficacy in patients aged less than 60 years with secondary acute myeloid leukemia(Blood Cancer Journal, 10.1038/s41408-025-01394-7) Calogero Vetro, Bruno Garibaldi, Francesco Grimaldi, Patrizia Chiusolo, Carla Filì, Carola Riva, Valeria Cardinali, Marinunzia Franciosa, Federica Pilo, Raffaele Palmieri, Lorenzo Esposito, Diego Menotti, Salvatore Perrone, Antonino Mulè, Caterina Alati, Ernesto Vigna, Michela Dargenio, Giulia De Luca, Alessandra Sperotto, Ilaria Tanasi, Cristina Papayannidis, Matteo Molica, Paola Minetto, Giorgia Battipaglia, Mara Memoli, Prassede Salutari, Lorenzo Brunetti, Elisabetta Todisco, Ivana Lotesoriere, Alessandro Isidori, Giuseppe Alberto Palumbo, Mario Annunziata, Michele Gottardi, Massimo Gentile, Maria Paola Martelli, Debora Capelli, Massimo Martino, Marco Rossi, Felicetto Ferrara, Adriano Venditti, Claudio Romani, Sara Galimberti, Anna Candoni, Luana Fianchi, Livio Pagano, Giovanni Marconi, Roberto Massimo Lemoli, Francesco Di Raimondo, Atto Billio, Fanny Erika Palumbo, Fabrizio Pane, Andrea Duminuco, Fabio Guolo Blood Cancer Journal, 2025 In this article, the author Lorenzo Esposito should have affiliations 3 and 11 and Massimo Gentile should have affiliations 16 and 27. The name De De Luca should be corrected to Giulia De Luca Lorenzo Brunetti has an incorrect affiliation. The correct affiliations are: a) Dipartimento di Scienze Cliniche e Molecolari, Università Politecnica delle Marche, Ancona, Italy b) Clinica di Ematologia, Azienda Ospedaliero Universitaria delle Marche, Ancona, Italy Debora Capelli also has an incorrect affiliation. The correct one is: Clinica di Ematologia, Azienda Ospedaliero Universitaria delle Marche, Ancona, Italy Additionally, affiliations 6 and 31 in the current manuscript should therefore be merged, as they refer to the same institution.
PNH clones prevalence study in ph-negative myeloproliferative neoplasms: a multicenter Italian study Alessandra D’Addio, Michela Rondoni, Marzia Salvucci, Giovanni Marconi, Massimiliano Bonifacio, Ilaria Tanasi, Omar Perbellini, Giuseppe Carli, Patrizia Tosi, Simona Tomassetti, Giovanni Poletti, Evita Massari, Marco Rosetti, Elisabetta Fabbri, Chiara Zingaretti, Alessandro Lucchesi, Maria Teresa Bochicchio, Giorgia Simonetti, Mauro Krampera, Francesco Lanza Annals of Hematology, 2025 The prevalence of paroxysmal nocturnal hemoglobinuria (PNH) clones is little investigated in myeloproliferative neoplasms (MPN) patients. The aim of this multicenter study was to evaluate the prevalence of PNH clones (glycosyl-phosphatidyl-inositol lacking) in 119 Ph- negative MPN patients having anemia, LDH elevation, asthenia and history of thrombosis. All the participating centers performed the standardized diagnostic test by using a single lyophilized template for granulocytes, monocytes, and erythrocytes. Next generation sequencing (NGS) was performed in 2 PNH-positive MPN cases and 13 PNH-negative MPN. The prevalence of PNH positive clones was 3.23% (n. 3 patients). All three patients had splenomegaly; none of them had thrombosis. One patient affected by CALR mutated essential thrombocytopenia, had a small clone (0.52%), clinically irrelevant; one patient affected by JAK2 V617F primary myelofibrosis (PMF) showed a PNH clone of 89.8%, severe anemia and hemoglobinuria and started eculizumab therapy; the third patient affected by CALR mutated PMF showed a PNH clone of 92.6% but without severe anemia and breakthrough hemolysis and eculizumab therapy was not undertaken. PIGA deletion was detected in PNH-positive cases along with mutations of myeloid-related genes. These data seem to suggest an association of CALR mutation and JAK2 V617F mutation with PNH positive clones suggesting that the worsening of malignant process may be associated with the acquisition of multiple genetic mutations. Clinical Trial Registration : NCT06159816.
Safety and efficacy of extended venom immunotherapy maintenance intervals in mastocytosis Giovanna Sfriso, Matteo Martini, Maria Beatrice Bilò, Maria Angiola Crivellaro, Marina Mauro, Erminia Ridolo, Carla Lombardo, Maria Teresa Costantino, Marcello Cilia, Donatella Bignardi, Fabio Lodi Rizzini, Irene Martignago, Francesco Murzilli, Elisabetta Favero, Giovanni Marchi, Federico Reccardini, Elisa Boni, Valentina Gueli, Francesca Norelli, Ilaria Tanasi, Andrea Bernardelli, Francesco Olivieri, Patrizia Bonadonna Journal of Allergy and Clinical Immunology in Practice, 2025
New Combination Regimens vs. Fludarabine, Cytarabine, and Idarubicin in the Treatment of Intermediate- or Low-Risk Nucleophosmin-1-Mutated Acute Myeloid Leukemia: A Retrospective Analysis from 7 Italian Centers Giulia Battaglia, Davide Lazzarotto, Ilaria Tanasi, Carmela Gurrieri, Laura Forlani, Endri Mauro, Francesca Capraro, Giulia Ciotti, Eleonora De Bellis, Chiara Callegari, Luca Tosoni, Matteo Fanin, Gian Luca Morelli, Claudia Simio, Cristina Skert, Michele Gottardi, Francesco Zaja, Eleonora Toffoletti, Daniela Damiani, Renato Fanin, Mario Tiribelli Journal of Clinical Medicine, 2025 Background: Nucleophosmin-1 (NPM1) mutation accounts for 30% of acute myeloid leukemia (AML) cases and defines either low- or intermediate-risk AML, depending on FLT3-ITD mutation. New combination regimens (NCRs), adding midostaurin and gemtuzumab ozogamicin (GO) to the 3 + 7 scheme, are commonly used, though there are no data that compare NCRs with intensive induction chemotherapy. Methods: To evaluate the efficacy and safety of NCRs and FLAI in NPM1+ AML, we retrospectively analyzed 125 patients treated with FLAI (n = 53) or NCRs (n = 72) at seven Italian Centers. Results: The median age was 61 years and 51/125 (41%) were FLT3-ITD+. The complete remission (CR) rate was 77%, slightly better with NCRs (83% vs. 68%; p = 0.054). NCRs yielded a superior median overall survival (OS) (not reached (NR) vs. 27.3 months; p = 0.002), though the median event-free survival (EFS) was similar (NR vs. 20.5 months; p = 0.07). In low-risk AML, CR was higher in NCRs (94% vs. 72%, p = 0.02), as were median OS (NR vs. 41.6 months; p = 0.0002) and EFS (NR vs. 17.8 months; p = 0.0085). In intermediate-risk AML (FLT3-ITD+), there were no differences in CR (60% vs. 71%; p = 0.5), OS (p = 0.27), or EFS (p = 0.86); only allogeneic transplantation improved OS (NR vs. 13.4 months; p = 0.005), regardless of induction regimen. The safety profile was similar, except for delayed platelet recovery with FLAI (22 vs. 18 days; p = 0.0024) and higher-grade II–IV gastrointestinal toxicity with NCRs (43% vs. 18.8%; p = 0.0066). Conclusions: Our data suggest the superiority of NCRs over FLAI in low-risk patients, while all outcomes were comparable in intermediate-risk patients, a setting in which only transplants positively impacted on survival.
Safety of Drug Provocation Tests in Adults With and Without Clonal Mast Cell Disorders Francesca Nalin, Bianca Olivieri, Francesco Olivieri, Elisa Olivieri, Roberta Zanotti, Ilaria Tanasi, Andrea Bernardelli, Massimiliano Bonifacio, Francesca Norelli, Giorgia Marta, Valentina Gueli, Giovanna Sfriso, Patrizia Bonadonna Allergy European Journal of Allergy and Clinical Immunology, 2025 BackgroundMastocytosis is a clonal mast cell disorder (CMD) characterized by the proliferation and accumulation of mast cells (MC) in different tissues. Anaphylaxis, resulting from massive MC activation and mediators' release, is reported in 22%–49% of mastocytosis cases, with drugs being potential triggers. Proper counseling on drug safety is crucial. We aim to demonstrate that, after a careful evaluation of clinical history and allergic work‐up, drug provocation tests (DPT) are a safe and effective diagnostic tool in patients with CMD.MethodsWe enrolled 104 CMD patients with a suspicion of drug hypersensitivity reactions (DHR) or without known tolerated drugs and 100 control patients with DHR. The types of DHR and the results of DPT were compared between CMD and control groups.ResultsIn both groups, previous DHR was mostly represented by skin reactions (46.4% in CMD and 82.9% in the control group); the most involved drugs were aminopenicillins and nonsteroidal anti‐inflammatory drugs (NSAIDs). We performed 250 DPTs in the CMD group and 231 in the control group; challenges were well tolerated in both groups, except for 6 skin reactions: 1 in the CMD group (1.0%) and 5 in the control group (5%).ConclusionDrug challenge is a safe and effective diagnostic tool in patients with CMD. Moreover, patients that have never had adverse reactions to NSAIDs or antibiotics before the diagnosis of CMD do not need to undergo challenge tests. In contrast, patients with a history of reactions should avoid the culprit drugs and undergo DPT to identify a safe alternative drug.
Underlying systemic mastocytosis in patients with unexplained osteoporosis: score proposal Ilaria Tanasi, Lara Crosera, Francesco Taus, Giovanni Orsolini, Giovanni Adami, Francesco Olivieri, Andrea Bernardelli, Patrizia Bonadonna, Francesca Nalin, Stefania Sella, Sandro Giannini, Yihui Liu, Francesco Mannelli, Fiorenza Vanderwert, Massimiliano Bonifacio, Mauro Krampera, Maurizio Rossini, Jonathan J. Lyons, Roberta Zanotti Bone, 2024
BH3 mimetics in relapsed and refractory adult acute lymphoblastic leukemia: a Campus ALL real-life study Francesco Malfona, Ilaria Tanasi, Matteo Piccini, Cristina Papayannidis, Vincenzo Federico, Valentina Mancini, Elisa Roncoroni, Elisabetta Todisco, Simona Bianchi, Giulia Ciotti, Patrizia Chiusolo, Massimo Gentile, Valentina Gianfelici, Fabio Giglio, Michele Malagola, Antonino Mulé, Francesco Saraceni, Calogero Vetro, Francesco Zallio, Luca Vincenzo Cappelli, Giovanni Pizzolo, Robin Foà, Massimiliano Bonifacio, Sabina Chiaretti Haematologica, 2024
Blinatumomab differentially modulates peripheral blood and bone marrow immune cell repertoire: A Campus ALL study Darina Ocadlikova, Federico Lussana, Nicola Fracchiolla, Massimiliano Bonifacio, Lidia Santoro, Mario Delia, Sabina Chiaretti, Crescenza Pasciolla, Alessandro Cignetti, Fabio Forghieri, Francesco Grimaldi, Giulia Corradi, Letizia Zannoni, Stefania De Propris, Gian Maria Borleri, Ilaria Tanasi, Jayakumar Vadakekolathu, Sergio Rutella, Anna Rita Guarini, Robin Foà, Antonio Curti, and British Journal of Haematology, 2023
Real-world efficacy and safety of luspatercept and predictive factors of response in patients with lower risk myelodysplastic syndromes with ring sideroblasts Luca Lanino, Francesco Restuccia, Alessandra Perego, Marta Ubezio, Bruno Fattizzo, Marta Riva, Angela Consagra, Pellegrino Musto, Daniela Cilloni, Esther Natalie Oliva, Raffaele Palmieri, Antonella Poloni, Catello Califano, Isabella Capodanno, Federico Itri, Chiara Elena, Claudio Fozza, Fabrizio Pane, Anna Maria Pelizzari, Massimo Breccia, Francesco Di Bassiano, Elena Crisà, Dario Ferrero, Valentina Giai, Daniela Barraco, Antonella Vaccarino, Davide Griguolo, Paola Minetto, Martina Quintini, Stefania Paolini, Grazia Sanpaolo, Mariarosaria Sessa, Monica Bocchia, Nicola Di Renzo, Elisa Diral, Livia Leuzzi, Angelo Genua, Attilio Guarini, Alfredo Molteni, Barbara Nicolino, Ubaldo Occhini, Giulia Rivoli, Roberto Bono, Anna Calvisi, Andrea Castelli, Eros Di Bona, Ambra Di Veroli, Felicetto Ferrara, Luana Fianchi, Sara Galimberti, Daniele Grimaldi, Monia Marchetti, Marianna Norata, Marco Frigeni, Rosaria Sancetta, Carmine Selleri, Ilaria Tanasi, Patrizia Tosi, Mauro Turrini, Laura Giordano, Carlo Finelli, Paolo Pasini, Ilaria Naldi, Valeria Santini, Matteo Giovanni Della Porta, and American Journal of Hematology, 2023
Mast cell leukemia: clinical and molecular features and survival outcomes of patients in the ECNM Registry Vanessa E. Kennedy, Cecelia Perkins, Andreas Reiter, Mohamad Jawhar, Johannes Lübke, Hanneke C. Kluin-Nelemans, William Shomali, Cheryl Langford, Justin Abuel, Olivier Hermine, Marek Niedoszytko, Aleksandra Gorska, Andrzej Mital, Patrizia Bonadonna, Roberta Zanotti, Ilaria Tanasi, Mattias Mattsson, Hans Hagglund, Massimo Triggiani, Akif Selim Yavuz, Jens Panse, Deborah Christen, Marc Heizmann, Khalid Shoumariyeh, Sabine Müller, Chiara Elena, Luca Malcovati, Nicolas Fiorelli, Friederike Wortmann, Vladan Vucinic, Knut Brockow, Christos Fokoloros, Sotirios G. Papageorgiou, Christine Breynaert, Dominique Bullens, Michael Doubek, Anja Ilerhaus, Irena Angelova-Fischer, Oleksii Solomianyi, Judit Várkonyi, Vito Sabato, Axel Rüfer, Tanja Daniela Schug, Maud A. W. Hermans, Anna Belloni Fortina, Francesca Caroppo, Horia Bumbea, Theo Gulen, Karin Hartmann, Hanneke Oude Elberink, Juliana Schwaab, Michel Arock, Peter Valent, Wolfgang R. Sperr, Jason Gotlib Blood Advances, 2023
Incidence, treatment and outcome of central nervous system relapse in adult acute lymphoblastic leukaemia patients treated front-line with paediatric-inspired regimens: A retrospective multicentre Campus ALL study Michelina Dargenio, Massimiliano Bonifacio, Sabina Chiaretti, Antonella Vitale, Nicola Stefano Fracchiolla, Cristina Papayannidis, Fabio Giglio, Prassede Salutari, Ernesta Audisio, Barbara Scappini, Patrizia Zappasodi, Marzia Defina, Fabio Forghieri, Anna Maria Scattolin, Elisabetta Todisco, Monia Lunghi, Fabio Guolo, Maria Ilaria Del Principe, Mario Annunziata, Davide Lazzarotto, Michele Cedrone, Crescenza Pasciolla, Annalisa Imovilli, Ilaria Tanasi, Silvia Trappolini, Marco Cerrano, Roberta La Starza, Mauro Krampera, Nicola Di Renzo, Anna Candoni, Giovanni Pizzolo, Felicetto Ferrara, Robin Foà British Journal of Haematology, 2023
Daratumumab with or without chemotherapy in relapsed and refractory acute lymphoblastic leukemia. A retrospective observational Campus ALL study Marco Cerrano, Massimiliano Bonifacio, Matteo Olivi, Antonio Curti, Michele Malagola, Michelina Dargenio, Anna Maria Scattolin, Cristina Papayannidis, Fabio Forghieri, Carmela Gurrieri, Ilaria Tanasi, Patrizia Zappasodi, Roberta La Starza, Nicola Stefano Fracchiolla, Patrizia Chiusolo, Luisa Giaccone, Maria Ilaria Del Principe, Fabio Giglio, Marzia Defina, Claudio Favre, Carmelo Rizzari, Barbara Castella, Giovanni Pizzolo, Felicetto Ferrara, Sabina Chiaretti, Robin Foà Haematologica, 2022
The serological prevalence of SARS-CoV-2 infection in patients with chronic myeloid leukemia is similar to that in the general population Massimiliano Bonifacio, Mario Tiribelli, Maria Cristina Miggiano, Elisabetta Abruzzese, Gianni Binotto, Luigi Scaffidi, Maddalena Cordioli, Daniela Damiani, Eros Di Bona, Malgorzata Monika Trawinska, Ilaria Tanasi, Maria Vittoria Dubbini, Vanessa Velotta, Giulia Ceccarelli, Elisabetta Pierdomenico, Mariella Lo Schirico, Gianpietro Semenzato, Marco Ruggeri, Renato Fanin, Evelina Tacconelli, Giovanni Pizzolo, Mauro Krampera Cancer Medicine, 2021
Familial occurrence of systemic and cutaneous mastocytosis in an adult multicentre series Ilaria Tanasi, Massimiliano Bonifacio, Miriam Pizzolato, Federica Irene Grifoni, Mariarita Sciumè, Chiara Elena, Pietro Benvenuti, Francesco Mannelli, Roberta Parente, Donatella Schena, Luigi Scaffidi, Patrizia Bonadonna, Cristina Papayannidis, Michela Rondoni, Marianna Criscuolo, Alessandro M. Vannucchi, Massimo Triggiani, Giovanni Martinelli, Mauro Krampera, Roberta Zanotti British Journal of Haematology, 2021
Proposed global prognostic score for systemic mastocytosis: a retrospective prognostic modelling study Javier I Muñoz-González, Iván Álvarez-Twose, María Jara-Acevedo, Roberta Zanotti, Cecelia Perkins, Mohamad Jawhar, Wolfgang R Sperr, Khalid Shoumariyeh, Juliana Schwaab, Georg Greiner, Ana Henriques, Carolina Caldas, Carlos Fernández-Giménez, Laura Sánchez-Muñoz, Andrea Mayado, Alba Pérez-Pons, Annette Schmitt-Graeff, Justus Duyster, Ilaria Tanasi, Francesco Olivieri, Elvira Mora-Casterá, Irene Luna, Leonor Senent, Maria-Helena Bañas, Amanda Nuñez-García, Manuel Jurado-Chacón, Guillermo Martín-Sánchez, Enrique Colado, Blanca Xicoy, Georgina Gener-Ricós, Jason Gotlib, Patrizia Bonadonna, Andreas Reiter, Peter Valent, Andrés C García-Montero, Alberto Orfao Lancet Haematology, 2021