Huda Dhaher Hathal

Assistant professor, interest in Medical parasitology.

EDUCATION

Ph.D in Medical parasitology ,Collage of Medicine, Microbiology university,2002
MSc. in Biology .Collage of Science ,
BSc. in General biology,Collage of Science , university,1988

RESEARCH INTERESTS

In Medical Microbiology(Parasitology , Bacteriology , Virology ),Immunology, Molecular, Disease Epidemiology ,Diagnosis and prevention

Scopus Publications

Potential effects of toxoplasmosis on Toll‑like receptor‑5 gene polymorphisms (rs5744168, rs2072493) in a sample of patients undergoing hemodialysis
Huda Al‑Marsomy, Kareem Mouhammed, Adel Al‑Baldawy, Nadia Mohammed
World Academy of Sciences Journal, 2024
Toxoplasma gondii (T. gondii; toxoplasmosis) causes latent infection that is activated in immunocompromised patients, such as in patients undergoing hemodialysis. The aim of the present study was to examine the association between TLR‑5 gene polymorphisms in a sample of patients undergoing hemodialysis who were positive for toxoplasmosis determined using quantitative‑polymerase chain reaction techniques. The present case‑control study was performed on 100 patients undergoing hemodialysis. The study included 50 seropositive patients [immunoglobulin (Ig)M and IgG] for T. gondii (group1) and a control group of 50 patients undergoing hemodialysis who were seronegative (IgM and IgG) for T. gondii (group 2). Toxoplasma antibodies were detected using ELISA, and Toll‑like receptor (TLR)‑5 gene polymorphisms (rs5744168 and rs2072493) were detected using qPCR. Group 1 included 18 patients who had a higher heterozygous genotype (GA) frequency (36%) of the TLR‑5 gene polymorphism (rs5744168) than group 2 10 (20%). However, the difference was not statistically significant (odds ratio, 2.25; 95% confidence interval (CI), 0.91 to 5.54; P=0.0781). The same was true for the frequency of the mutant allele (allele A) in groups 1 and 2 (9 vs. 10%, respectively), with no significant differences (OR, 0.89; 95% CI, 0.34 to 2.29; P=0.80). However, the frequency of the heterozygous genotype (TC) of the TLR‑5 gene polymorphism (rs2072493) remained similar in group 1 and group 2 (both 20 (40%)) with no statistically significant differences (OR, 1.00; 95% CI, 0.44 to 2.22; P>0.999). The frequency of the mutant allele (allele C) appeared in both groups (group 1, 30%; group 2, 20%), exhibiting no statistically significant differences (OR, 1.71; CI, 0.89 to 3.28; P=0.1043). All these values were at the allelic level. A positive correlation was observed for rs5744168 in groups 1 and 2 with positively correlated, whereas a negative correlation was observed for rs2072493 in groups 1 and 2. On the whole, the present study demonstrates that is no statistically significant association between toxoplasmosis and mutant alleles (alleles A and C) in patients undergoing hemodialysis.
TLR-4 Polymorphisms Shows The Genetic Susceptibility to Toxoplasmosis and Polycystic Ovary Syndrome
Haider Iskandar Flayh, AlMarsomy, Huda Dhaher, Khazaali, Enas Adnan Abdulrasol
Journal of Angiotherapy, 2024
Background: Toxoplasmosis, caused by Toxoplasma gondii, presents asymptomatic in healthy individuals but can lead to severe outcomes in immunocompromised individuals. Toll-like Receptors (TLRs) are crucial in recognizing pathogens like T. gondii. Specifically, TLR-4 gene polymorphisms (rs4986790, rs4986791) may influence susceptibility to toxoplasmosis and polycystic ovary syndrome (PCOS). Methods: A case-control study of 150 women categorized into PCOS + toxoplasmosis, PCOS only, toxoplasmosis only, and control groups. TLR-4 SNPs were examined using ARMS PCR. Results: TLR-4 rs4986790 showed no significant difference between PCOS + toxoplasmosis and PCOS only but correlated significantly with toxoplasmosis alone. Rs4986791 showed no significant correlation. Notably, the AG genotype of rs4986790 was more frequent among toxoplasmosis patients than controls, suggesting susceptibility. Discussion: Limited global research on this topic indicates conflicting findings. The rs4986790 SNP may affect TLR4 function, potentially through altered signaling pathways or ligand binding. Mutant TLR4's conformational changes could disrupt ligand docking and signaling pathways, reducing the immune response. Conclusion: The presence of the G allele within TLR-4 rs4986790 polymorphism may decrease TLR-4's interaction with T. gondii, reducing the immune response and increasing susceptibility to infection among AG genotype carriers.
Prevalence of toxoplasma gondii infection among sample of Iraqi adolescents
Sarah Qasim Abdul-hussein, Huda Dhaher Al-Marsomy
Annals of Tropical Medicine and Public Health, 2020
Toxoplasma gondii is an obligate intracellular parasite that can cause variable clinical symptoms or can even be asymptomatic in immunocompetent individuals. More severe symptoms are observed in immunocompromised patients. The present study was aimed to estimate the prevalence of toxoplasmosis in some Iraqi samples of adolescents and to study the association between some social factors and the disease frequency. A cross-sectional study was conducted on 750 blood samples collected from persons attended to the hospital (males and females) aged between (10-19) years old, in the period from January 2019 to April 2019. The persons were recruited from AlMahmudiya Hospital and Al-Yarmouk hospital /Baghdad/Iraq. All persons were investigated for the presence of Toxoplasma gondii by Rapid chromatographic technique and for IgG titer by Enzyme-Linked Immunosorbent Assay. The overall prevalence of T.gondii IgG by ELISA and the rapid test was (40.65%and 12.8%), respectively. Prevalence of Toxoplasmosis according to the age revealed that the older age class (18-19 years) was mostly affected 29.47% of the total population followed by age class 14-15 years and 16-17 years which had almost similar percentage (18.67% and 18.53% respectively). The Youngest classes 10-11 and 12-13 came last accounting for only 14.8% and 14.67% respectively of the study population. Females were more frequent than males representing 60% versus 40% of the population. One of the reasons for this high prevalence is related to the fact that women handle raw meat more frequently than men due to the fact that they spend more time cooking at home. In conclusion, the results of this study in Iraqi adolescents indicate the 40.65% of them had exposed to the parasite and this needs more attention for increasing the awareness about the disease.
Association between trichomonas vaginalis and vaginal bacterial community composition in human vagina
Huda Dhaher Al-Marsomy
Research Journal of Pharmacy and Technology, 2020
Study the role of trichomoniasis in in vitro fertilization (IVF) failure
Medico Legal Update, 2020
Diagnosis of Trichomonas Vaginalis infection by detection of glutaminase (Glut) Gene by nested PCR
Faten Ahmed Hassan, Huda Dhaher Al-Marsomy, Suhad Asaad Mustafa
Indian Journal of Public Health Research and Development, 2019
Trichomonas vaginalis (T. vaginalis) is a common pathogen with a cosmopolitan distribution. Trichomoniasis is associated with vaginitis, cervicitis, low birth weight, preterm delivery, and infertility. Glutaminase (glut) p3 gene which is a housekeeping gene increases the detection of T. vaginalis efficiency when used as a marker of Trichomoniasis. In this study (glut) P3 gene was depended for parasite detection by nested PCR and compared with conventional methods like wet mount and culture in the detection of T. vaginalis in vaginal discharge. Two vaginal swab specimens were obtained from 157 cases, of (mean age = 31.79 ± 9.21 years), both symptomatic and symptomatic females attending Gynecology OPD of Al-Emamayn Al-Kadhimayn Medical City in Baghdad and Maternity Teaching hospital in Erbil province, Iraq. One swab was immediately examined by wet mount microscopy and then placed in CPLM medium for cultivation, and another swab is placed in PBS buffer for PCR method. A total of 100 samples positive in one or more tests were identified: 20 (12.7%) infections were detected by wet mount microscopy, and 30 (19.1%) positives in culture. P3 nested PCR was positive in 100 (63.7%) samples. PCR appears to be the most sensitive method with high detection rate and method of choice. Furthermore nested PCR increases the sensitivity and specificity of PCR, and Glutaminase (glut) p3 gene can be efficiently used for detection of T.vaginalis.
The association of Glutaminase (Glut) P3 different alleles of trichomonas vaginalis with infertility in a sample of Iraqi women
Prensa Medica Argentina, 2019
Molecular detection for trichomonas virginals in placenta tissue from women with bod
Huda Dhaher Al-Marsomy
International Journal of Research in Pharmaceutical Sciences, 2018