Effects of cysteine supplementation on the quality of cryopreserved sperm of South American silver catfish Bruna Bitencourt Da Costa, Lis Santos Marques, Paula Graziela Lassen, Rômulo Batista Rodrigues, Helen Tais Da Rosa Silva, José Cláudio Fonseca Moreira, Danilo Pedro Streit Aquaculture Research, 2020 The cryopreservation promotes cellular damage that could compromise sperm quality in terms of motility and fertility rates, which may be caused by oxidative stress. Thus, the aim of this study was to assess the effects of cysteine addition on post‐thaw sperm quality, DNA damage and indices of oxidative stress of the South American silver catfish (Rhamdia quelen) sperm, compared with the cryoprotectant solution without cysteine addition. Sperm collected from five males were cryopreserved in cryoprotectant solution (fructose 50 g/L, powdered milk 50 g/L and methanol 100 ml/L) containing different cysteine concentrations (0, 2.5, 5, 10 and 20 mM). After thawing, the following were measured: sperm motility, morphology, sperm viability, DNA damage, lipid peroxidation, concentration of carbonyl and sulfhydryl groups and the activity of SOD, CAT, GST and GPx enzymes. The lowest sperm motility was determined for semen cryopreserved with addition of 20 mM of cysteine. The control group had the lowest DNA damage and lipid peroxidation. The findings of this study show that cysteine addition had no positive effect on evaluated parameters. Therefore, the concentrations tested are not recommended for the supplementation of cryoprotectant solution for semen of R. quelen.
Slow freezing versus vitrification for the cryopreservation of zebrafish (Danio rerio) ovarian tissue Lis S. Marques, Ana A. N. Fossati, Rômulo B. Rodrigues, Helen T. Da Rosa, Aryele P. Izaguirry, Juliana B. Ramalho, José C. F. Moreira, Francielli Weber Santos, Tiantian Zhang, Danilo P. Streit Scientific Reports, 2019 The aim of the present study was to compare the efficiency of vitrification and slow freezing techniques for the cryopreservation of zebrafish ovarian tissue containing immature follicles. In Experiment 1, assessment of cell membrane integrity by trypan blue exclusion staining was used to select the best cryoprotectant solution for each cryopreservation method. Primary growth (PG) oocytes showed the best percentage of membrane integrity (63.5 ± 2.99%) when SF4 solution (2 M methanol + 0.1 M trehalose + 10% egg yolk solution) was employed. The vitrification solution, which presented the highest membrane integrity (V2; 1.5 M methanol + 5.5 M Me2SO + 0.5 M sucrose + 10% egg yolk solution) was selected for Experiment 2. Experiment 2 aimed to compare the vitrification and slow freezing techniques in the following parameters: morphology, oxidative stress, mitochondrial activity, and DNA damage. Frozen ovarian tissue showed higher ROS levels and lower mitochondrial activity than vitrified ovarian tissue. Ultrastructural observations of frozen PG oocytes showed rupture of the plasma membrane, loss of intracellular contents and a large number of damaged mitochondria, while vitrified PG oocytes had intact mitochondria and cell plasma membranes. We conclude that vitrification may be more effective than slow freezing for the cryopreservation of zebrafish ovarian tissue.
The effects of retinol oral supplementation in 6-hydroxydopamine dopaminergic denervation model in Wistar rats Alice Kunzler, Camila Tiefensee Ribeiro, Juciano Gasparotto, Lyvia Lintzmaier Petiz, Helen Thais da Rosa Silva, Jeferson Delgado da Silva, Rafael Bortolin, Priscila Oliveira de Souza, Fabiano Barreto, Pedro Espitia-Perez, Carlos Eduardo Schnorr, Nauana Somensi, José Cláudio Fonseca Moreira, Daniel Pens Gelain Neurochemistry International, 2019
Origins of the middle meningeal artery and its probable embryological mechanism - A review Journal of Morphological Sciences, 2013
Sexual dimorphism in the human vocal fold innervation Deivis de Campos, Joel Henrique Ellwanger, Patrícia Severo do Nascimento, Helen Tais da Rosa, Lisiani Saur, Geraldo Pereira Jotz, Léder Leal Xavier Journal of Voice, 2013
