Anatomical pathology, surgical pathology, medical education
20
Scopus Publications
580
Scholar Citations
15
Scholar h-index
15
Scholar i10-index
Scopus Publications
Parthenolide ameliorates 3-nitropropionic acid-induced Huntington’s disease-like aberrations via modulating NLRP3 inflammasome, reducing microglial activation and inducing astrocyte shifting Mona E. Noureldeen, Nancy N. Shahin, Hebat Allah A. Amin, Maha M. El-Sawalhi, Heba R. Ghaiad Molecular Medicine, 2024 Background Huntington’s disease (HD) is a progressive neurodegenerative disease that causes motor, cognitive, and psychiatric abnormalities, with no satisfying disease-modifying therapy so far. 3-nitropropionic acid (3NP) induces behavioural deficits, together with biochemical and histological alterations in animals’ striata that mimic HD. The role of nucleotide-binding domain, leucine-rich–containing family, pyrin domain–containing-3 (NLRP3) inflammasome in HD pathogenesis remains largely uncharacterized. Parthenolide (PTL), a naturally occurring nuclear factor kappa B (NF-κB) inhibitor, is also known to inhibit NLRP3 inflammasome. Whether PTL is beneficial in HD has not been established yet. Aim This study evaluated the possible neuroprotective effects of PTL against 3NP-induced behavioural abnormalities, striatal biochemical derangements, and histological aberrations. Methods Male Wistar rats received PTL (0.5 mg/kg/day, i.p) for 3 weeks and 3NP (10 mg/kg/day, i.p) was administered alongside for the latter 2 weeks to induce HD. Finally, animals were subjected to open-field, Morris water maze and rotarod tests. Rat striata were examined histologically, striatal protein expression levels of glial fibrillary acidic protein (GFAP), cluster of differentiation 45 (CD45) and neuron-specific enolase (NSE) were evaluated immunohistochemically, while those of interleukin (IL)-1β, IL-18, ionized calcium-binding adapter molecule-1 (Iba1) and glutamate were determined by ELISA. Striatal nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein (Keap1), NF-κB, NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC), caspase-1, S100 calcium-binding protein A10 (S100A10) and complement-3 (C3) were assessed by gene expression analysis. Results PTL improved motor, locomotor, cognitive and anxiety-like behaviours, restored neuronal integrity, upregulated Nrf2, and inhibited NLRP3 inflammasome, NF-κB and microglial activation. Additionally, PTL induced astrocyte shifting towards the neuroprotective A2 phenotype. Conclusion PTL exhibits neuroprotection against 3NP-induced HD, that might be ascribed, at least in part, to its modulatory effects on Keap1/Nrf2 and NF-κB/NLRP3 inflammasome signaling.
Bone marrow embolism: should it result from traumatic bone lesions? A histopathological human autopsy study Maha Farid, Esraa Zohny, Alaa Ismail, Mariem Ateya, Ahmed Abdel-Razek, et al. Forensic Science Medicine and Pathology, 2024 Bone marrow embolism (BME) is likely a consequence of fractures in which pulmonary vessels are the most affected. However, some cases of BME were reported in the absence of trauma. Thus, a traumatic injury might not be necessary for developing BME. This study discusses BME cases in patients without signs of fractures or blunt trauma. The discussion addresses various possible mechanisms for the appearance of BME. Options include cancer in which bone marrow metastasis is a suggestive cause. Another proposal is the chemical theory where bone marrow fats are released via lipoprotein lipase in a pro-inflammatory state, resulting in vascular/pulmonary obstruction. Other cases discussed in this study are hypovolemic shock and drug-abuse related BME. All autopsy cases with BME were included regardless of the cause of death for a period of 2 years. Autopsies involved complete dissection with the macroscopic evaluation of the affected organs, including the heart, lungs, and brain. Tissues were also prepared for microscopic examination. Of the 11 cases, eight showed non-traumatic BME (72%). These findings conflict with theories in the literature that BME most commonly occurs after fractures or trauma. One of the eight cases exhibited mucinous carcinoma; one is presented with hepatocellular carcinoma; and two cases showed severe congestion. Lastly, one case was found to be associated with each of the following conditions: liposuction, drug abuse, pulmonary hypertension, and heart failure. Each case suggests a different pathophysiology for developing BME, yet the exact mechanisms are not fully understood. Further study of non-traumatic associated BME is recommended.
Cultural Appropriation for Improved Knowledge Acquisition in Medical Education Samar Ahmed, Nagwa N Hegazy, Hebat Allah Amin, Mariam Ismail, Yasser Alakid, et al. Advances in Medical Education and Practice, 2024 Background Culture is an essential component that governs all aspects of human behavior. Superstition is an irrational belief observed in almost all cultures. It is linked to one or more factors like supernatural powers, good luck, bad omen, fiction, illegitimate activity, absurd narration, folk tales, or practice without any rational basis. Methods A cross-sectional social experiment was conducted to evaluate the effect of cultural appropriation as a tool to enhance medical knowledge acquisition and attitudinal development in medical education. The experiment was designed to target a non-medical population. Four superstition-oriented videos were developed with 20 scientific pieces of information related to forensic medicine. A data collection sheet was developed on Microsoft form with 16 questions was distributed on the participants. Results Out of the 986 participants, 763 (77.5%) watched the whole set of videos. About 55–95% of responders demonstrated knowledge acquisition of all the questions. There was a statistically significant difference between those who watched the videos and those who did not. When participants were asked about the most important information they remember from the videos, their answers fell into two main categories; information related to core scientific knowledge (80% of participants) and information not related to the core knowledge (16% of respondents). The top three areas for the reasons why people wanted to watch the videos were curiosity, knowledge, and career. A change in attitudes was reported among the participants where 80% of responders demonstrated curiosity to know more about this world, 46% responders reported developing more respect for the forensic physician and 43% revealed their ignorance about this great hidden world. Conclusion Cultural appropriation could be a needed strategy to accommodate for upscale in education. Learners might validate that learning happens through a door that adopts not only honours their culture and adapts to it.
Ameliorating effect of ketogenic diet on acute status epilepticus: Insights into biochemical and histological changes in rat hippocampus Nagwa I. Shehata, Mai A. Abdelsamad, Hebat Allah A. Amin, Nermin A. H. Sadik, Amira A. Shaheen Journal of Food Biochemistry, 2022 This study aimed to evaluate the potential neuroprotective effects of ketogenic diet (KD) against the neuronal disruptions induced by SE in lithium-pilocarpine rat model of status epilepticus (SE). Four groups of female rats include; groups I and III received standard diet and groups II and IV received KD for 3 weeks. Groups I and II were left untreated, while groups III and IV were injected with LiCl (127 mg/kg, i.p.) followed by pilocarpine HCl (10 mg/kg, i.p.) 18-24 h later, repeatedly, till induction of SE. 72 h post-SE, KD effectively ameliorated the balance between excitatory (glutamate) and inhibitory (GABA) neurotransmitters and the oxidative stress indices, increased adenine nucleotides and decreased immunoreactivity of iNOS, TNFα, glial fibrillary acidic protein, and synaptophysin. Thiswas in association with improvement in inflammatory response and neuronal tissue characteristics in hippocampus of SE rats. Histological changes showed preservation of neuronal integrity. These findings highlight the protective effects of KD in the acute phase post-SE via ameliorating biochemical and histological changes involved. PRACTICAL APPLICATIONS: Epilepsy is the fourth most common neurological disorder that requires lifelong treatment. It stigmatizes patients and their families. The use of the ketogenic diet (KD) as a therapy for epilepsy developed from observations that fasting could reduce seizures. From 1920s, the KD was a common epilepsy treatment until it was gradually superseded by anticonvulsant drugs so that by the 1980s it was rarely used. However, there has been a resurgence of interest and usage of the KD for epilepsy since the turn of the century. Despite its long history, the mechanisms by which KD exhibits its anti-seizure action are not fully understood. Our study aims to identify the mechanism of KD which may help further studies to achieve the same benefits with a drug or supplement to overcome its unpalatability and gastrointestinal side effects.
Lutein exerts its cardioprotective effect against the experimental model of isoprenaline-induced myocardial infarction via MIAT/miR-200a/Nrf2/TXINP pathway Maha Abdelmonem, Sherehan M. Ibrahim, Reham M. Essam, Hebat Allah A. Amin, Mai A. Abd‐Elmawla Journal of Biochemical and Molecular Toxicology, 2021 Acute myocardial infarction (AMI) remains a leading cause of morbidity and mortality worldwide. Lutein (LU) possesses numerous pharmacological activities, including anti‐inflammatory, antioxidant, and antiapoptotic effects. This study aimed to investigate the cardioprotective potential of LU in isoprenaline (ISO)‐induced MI and to explore its molecular mechanisms of action. AMI was induced by two consecutive subcutaneous doses of ISO (65 mg/kg; s.c.). The LU group was pretreated with LU (20 mg/kg; p.o.) for 30 days followed by ISO injections on Days 29 and 30. ISO group showed elevated serum creatine kinas‐MB (CK‐MB) and considerable electrocardiographic changes along with reduced ejection fraction compared to the normal group. LU pretreatment could decrease serum CK‐MB activity, normalize QRS and QTc intervals and restore ejection fraction compared to the untreated group. The ISO group demonstrated infarcted‐like lesions, which were ameliorated in the LU‐pretreated group. Immunohistochemical investigation revealed upregulated cardiac troponin T (cTn T) and desmin expressions in the LU‐pretreated group. LU pretreatment also enhanced cardiac thioredoxin (Trx) and glutathione (GSH) contents as well as reduced lipid peroxidation, compared to the untreated group. Importantly, LU pretreatment could downregulate long noncoding MI associated transcript (lncRNA MIAT) and thioredoxin‐interacting protein (TXNIP) and augment micro RNA (miR)‐200a and nuclear factor erythroid 2‐related factor 2 (Nrf2) expressions compared to the ISO group. Moreover, a significant inverse correlation between MIAT and miR‐200a was observed. In conclusion, this study revealed that LU could ameliorate ISO‐induced MI in rats by modulating MIAT/miR‐200a/Nrf2 pathway.
Role of a schistosoma haematobium specific microRNA as a predictive and prognostic tool for bilharzial bladder cancer in Egypt Dalia A. Gaber, Rita M. Wassef, Wael M. El-Ayat, Mohamed I. El-Moazen, Karim A. Montasser, et al. Scientific Reports, 2020 Urinary bladder cancer is a common malignancy in Egypt, thus reliable methodologies are required for screening and early detection. In this study, we analyzed the gene expression of a Schistosoma hematobium specific microRNA “Sha-miR-71a” and mitogen-associated protein kinase-3 (MAPK-3) in the urine samples of 50 bladder cancer patients and 50 patients with benign bilharzial cystitis. Fifty control subjects were also tested. Indirect hemagglutination test (IHA) diagnosed 70% of studied cancer cases as bilharzial associated bladder cancer (BBC), while histopathological examination detected only 18%. Urinary Sha-miR-71a & MAPK-3 revealed enhanced expression in BBC (p-value = 0.001) compared to non-bilharzial bladder cancer (NBBC) cases. Patients with chronic bilharzial cystitis exhibited a significant increase in gene expression compared to those with acute infection (p-value = 0.001). Sha-miR-71a and MAPK-3 showed good sensitivity and specificity in the diagnosis of BBC when analyzed by the receiver operating characteristic (ROC) curve. They were also prognostic regarding malignancy grade. Both biomarkers showed a positive correlation. Our results revealed that IHA is a reliable test in the diagnosis of bilharziasis associated with bladder cancer, and that Sha-miR-71a and MAPK-3 provide non-invasive specific biomarkers to diagnose BBC, as well as a potential role in testing bilharzial patients for risk to develop cancer.
Cultural appropriation for improved knowledge acquisition in medical education SA Ahmed, NN Hegazy, HAA Amin, MIM Ismail, YF Alakid, AP Kumar Advances in Medical Education and Practice, 313-322 , 2024 2024 Citations: 5
Parthenolide ameliorates 3-nitropropionic acid-induced Huntington’s disease???like aberrations via modulating NLRP3 inflammasome, reducing microglial activation and inducing … MMESHRG Mona E. Noureldeen1 , Nancy N. Shahin , Hebat Allah A. Amin Molecular Medicine 30 (158) , 2024 2024 Citations: 27
Authors reply to Dr. Jakobsen comment on:“Bone marrow embolism: should it result from traumatic bone lesions? A histopathological human autopsy study” M Farid, E Zohny, A Ismail, M Ateya, A Abdel-Razek, N Hamed, ... Forensic Science, Medicine and Pathology 20 (3), 1109-1110 , 2024 2024
Immunoreactivity of Cardiac Troponin, Myoglobin, and Caspase 3 revealing the connection of Acute myocardial Infarction with Acute Lung Injury in autopsied cases of sudden … EI El Desouky, ME Sharaf, AW Torky, HAA Amin Ain Shams Journal of Forensic Medicine and Clinical Toxicology 43 (2), 14-37 , 2024 2024
Bone marrow embolism: should it result from traumatic bone lesions? A histopathological human autopsy study M Farid, E Zohny, A Ismail, M Ateya, A Abdel-Razek, N Hamed, ... Forensic Science, Medicine and Pathology 20 (2), 387-399 , 2024 2024 Citations: 6
Using a Multistage Qualitative Leadership Style Study to Identify Strategic Imperatives for Post COVID-19 Curricular Planning E Hala, A M El-ashkar, HA A Amin, S Ashry, H Jamjoom, S MM Omar, ... Educational Research and Innovation Journal 4 (13), 1-12 , 2024 2024
Exploring etiological causes of sudden unexpected death in autopsied cases of acute lung injury and associated acute myocardial injury in Egypt between 2009 and 2014: analysis … I EMAN, MELKM SHARAF, WT ABDELRAHMAN, HAA AMIN, AH KAMAR The Medical Journal of Cairo University 92 (03), 281-294 , 2024 2024 Citations: 1
The Utilization of Animals as Models for Human Autopsies in Medical Education, Supported by Peer-Assisted Tutoring RE Ashmawy, MH Shahin, AS Ghattas, MM Abdelwahab, LH Abdelwahab, ... Medical Science Educator 33 (2), 539-549 , 2023 2023 Citations: 1
Ameliorating effect of ketogenic diet on acute status epilepticus: Insights into biochemical and histological changes in rat hippocampus NI Shehata, MA Abdelsamad, HAA Amin, NAH Sadik, AA Shaheen Journal of food biochemistry 46 (9), e14217 , 2022 2022 Citations: 6
The Role of P16 Gene in the Diagnosis and Pathogenesis of HCC DA Gaber, O Shaker, M El-Kassas, S Sorour, HA Amin 2022
Expression of mesenchymal–epithelial transition factor in estrogen receptor-negative breast carcinoma DM Abouelfadl, NN Yassen, ME Shabana, HA Abdelal, HAA Amin Journal of The Arab Society for Medical Research 17 (1), 59-67 , 2022 2022 Citations: 1
Lutein exerts its cardioprotective effect against the experimental model of isoprenaline‐induced myocardial infarction via MIAT/miR‐200a/Nrf2/TXINP pathway M Abdelmonem, SM Ibrahim, RM Essam, HAA Amin, MA Abd‐Elmawla Journal of Biochemical and Molecular Toxicology 35 (11), e22899 , 2021 2021 Citations: 25
Using Appreciative Inquiry to explore the effect of COVID-19 on student trust: medical students’ perspective E Abouzeid, N Wasfy, S El-Zoghby, H Atwa, S Shalaby, N Zaghloul, ... MedEdPublish 9, 285 , 2021 2021 Citations: 3
Using appreciative inquiry to explore the disruptive effect of COVID-19 on medical student trust in their schools E Abouzeid, N Wasfy, S El-Zoghby, H Atwa, S Shalaby, N Zaghloul, ... MedEdPublish, 1-20 , 2021 2021 Citations: 4
Case item creation and video case presentation as summative assessment tools for distance learning in the pandemic era HAA Amin, H Khalil, D Khaled, M Mahdi, M Fathelbab, DA Gaber Medical Journal Armed Forces India 77, S466-S474 , 2021 2021 Citations: 24
Evaluating the effectiveness of faculty development program at the College of Medicine, University of Bisha AM El-Ashkar, EHI Miskeen, M Alghamdi, HAA Amin, M Hassanien MedEdPublish 10, 137 , 2021 2021 Citations: 6
Malpractice as a Leading Cause to Maternal Mortality in Egypt: A Postmortem Statistical Study S Ghaleb, M Kharoshah, H Barakat, H Amin, F Elroby Indian Journal of Forensic Medicine & Toxicology 15 (2), 856-864 , 2021 2021 Citations: 4
Role of a schistosoma haematobium specific microRNA as a predictive and prognostic tool for bilharzial bladder cancer in Egypt DA Gaber, RM Wassef, WM El-Ayat, MI El-Moazen, KA Montasser, ... Scientific Reports 10 (1), 18844 , 2020 2020 Citations: 15
A step-by-step guide to managing the educational crisis: lessons learned from COVID-19 pandemic SA Ahmed, MHK Shehata, RL Wells, HAA Amin, HSM Atwa Journal of Microscopy and Ultrastructure 8 (4), 193-197 , 2020 2020 Citations: 18
Immunomodulatory effect of diallyl sulfide on experimentally-induced benign prostate hyperplasia via the suppression of CD4+ T/IL-17 and TGF-β1/ERK pathways EM Elbaz, HAA Amin, AS Kamel, SM Ibrahim, HS Helmy Inflammopharmacology 28 (5), 1407-1420 , 2020 2020 Citations: 23
MOST CITED SCHOLAR PUBLICATIONS
Online Team‐ Based Learning Sessions as Interactive Methodologies During The Pandemic DA Gaber, MH Shehata, HAA Amin Medical Education. DOI:10.1111/medu.14198 , 2020 2020 Citations: 92
Role of rho kinase inhibition in the protective effect of fasudil and simvastatin against 3-nitropropionic acid-induced striatal neurodegeneration and mitochondrial dysfunction … LA Ahmed, HA Darwish, RM Abdelsalam, HAA Amin Molecular neurobiology 53 (6), 3927-3938 , 2016 2016 Citations: 65
Crosstalk between liver-related microRNAs and Wnt/β-catenin pathway in hepatocellular carcinoma patients AM Ashmawy, KM Elgeshy, ESTA Salam, M Ghareeb, MH Kobaisi, ... Arab journal of gastroenterology 18 (3), 144-150 , 2017 2017 Citations: 46
Meloxicam modulates oxidative stress status, inhibits prostaglandin E2, and abrogates apoptosis in carbon tetrachloride–induced rat hepatic injury M Edfawy, MH Hassan, A Mansour, AA Hamed, HAA Amin International Journal of Toxicology 31 (3), 276-286 , 2012 2012 Citations: 46
Step-by-step Guide to Create Competency-Based Assignments as an Alternative for Traditional Summative Assessment HAA Amin, MHK Shehata, SA Ahmed Mededpublish. DOI: 10.15694/mep.2020.000120.1 9 (1), 120 , 2020 2020 Citations: 37
Schistosomiasis and bladder cancer in Egypt: truths and myths HAA Amin, MH Kobaisi, RM Samir Open access Macedonian journal of medical sciences 7 (23), 4023 , 2019 2019 Citations: 30
Hydrogen sulfide enhances the effectiveness of mesenchymal stem cell therapy in rats with heart failure: In vitro preconditioning versus in vivo co-delivery M Abdelmonem, NN Shahin, LA Rashed, HAA Amin, AA Shamaa, ... Biomedicine & pharmacotherapy 112, 108584 , 2019 2019 Citations: 28
Parthenolide ameliorates 3-nitropropionic acid-induced Huntington’s disease???like aberrations via modulating NLRP3 inflammasome, reducing microglial activation and inducing … MMESHRG Mona E. Noureldeen1 , Nancy N. Shahin , Hebat Allah A. Amin Molecular Medicine 30 (158) , 2024 2024 Citations: 27
Lutein exerts its cardioprotective effect against the experimental model of isoprenaline‐induced myocardial infarction via MIAT/miR‐200a/Nrf2/TXINP pathway M Abdelmonem, SM Ibrahim, RM Essam, HAA Amin, MA Abd‐Elmawla Journal of Biochemical and Molecular Toxicology 35 (11), e22899 , 2021 2021 Citations: 25
Case item creation and video case presentation as summative assessment tools for distance learning in the pandemic era HAA Amin, H Khalil, D Khaled, M Mahdi, M Fathelbab, DA Gaber Medical Journal Armed Forces India 77, S466-S474 , 2021 2021 Citations: 24
Immunomodulatory effect of diallyl sulfide on experimentally-induced benign prostate hyperplasia via the suppression of CD4+ T/IL-17 and TGF-β1/ERK pathways EM Elbaz, HAA Amin, AS Kamel, SM Ibrahim, HS Helmy Inflammopharmacology 28 (5), 1407-1420 , 2020 2020 Citations: 23
Role of Cardiac Myocytes Heart Fatty Acid Binding Protein Depletion (H-FABP) in Early Myocardial Infarction in Human Heart (Autopsy Study) HAA Amany Shabaiek, Nour El-Hoda Ismael, Samar Elsheikh Open Access Macedonian Journal of Medical Sciences. , 2016 2016 Citations: 19
A step-by-step guide to managing the educational crisis: lessons learned from COVID-19 pandemic SA Ahmed, MHK Shehata, RL Wells, HAA Amin, HSM Atwa Journal of Microscopy and Ultrastructure 8 (4), 193-197 , 2020 2020 Citations: 18
Immuno-histochemistry in the detection of early myocardial infarction (a post-mortem study) HAA Amin, AMY El-Hennawy, GAA Nakhla, SAH Tabak, HH Hassan Egyptian Journal of Forensic Sciences 1 (1), 5-12 , 2011 2011 Citations: 16
Role of a schistosoma haematobium specific microRNA as a predictive and prognostic tool for bilharzial bladder cancer in Egypt DA Gaber, RM Wassef, WM El-Ayat, MI El-Moazen, KA Montasser, ... Scientific Reports 10 (1), 18844 , 2020 2020 Citations: 15
Lymphoepithelioma-Like Carcinoma of the Breast Mimicking Granulomatous Mastitis- Case Report and Review of the Literature. DM Abouelfadl, NN Yassin, HA Amin, ME Shabana The Asian Pacific Journal of Cancer Prevention (APJCP) 18 (7), Page 1737-1741 , 2017 2017 Citations: 9
Bone marrow embolism: should it result from traumatic bone lesions? A histopathological human autopsy study M Farid, E Zohny, A Ismail, M Ateya, A Abdel-Razek, N Hamed, ... Forensic Science, Medicine and Pathology 20 (2), 387-399 , 2024 2024 Citations: 6
Ameliorating effect of ketogenic diet on acute status epilepticus: Insights into biochemical and histological changes in rat hippocampus NI Shehata, MA Abdelsamad, HAA Amin, NAH Sadik, AA Shaheen Journal of food biochemistry 46 (9), e14217 , 2022 2022 Citations: 6
Evaluating the effectiveness of faculty development program at the College of Medicine, University of Bisha AM El-Ashkar, EHI Miskeen, M Alghamdi, HAA Amin, M Hassanien MedEdPublish 10, 137 , 2021 2021 Citations: 6
Students' awareness as an underlying factor for satisfaction and compliance OG Goda, DT Mahdy, MM El Shahed, HAA Amin, SA Ahmed Journal of Microscopy and Ultrastructure 8 (4), 198-204 , 2020 2020 Citations: 6