Harshita Jaiswal

@paruluniversity.ac.in

Assistant Professor, Department at Pharmacy Practice, School of Pharmacy,Parul University
School of Pharmacy, Parul University

Harshita Jaiswal


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https://researchid.co/harshita249

EDUCATION

Pharm. D (Doctrate of Pharmacy)

RESEARCH, TEACHING, or OTHER INTERESTS

Pharmacy, Medicine, Infectious Diseases, Obstetrics and Gynecology
4

Scopus Publications

Scopus Publications

  • Ofloxacin, paracetamol and cefixime induced Stevens-Johnson syndrome - toxic epidermal necrolysis in an adult female patient: a case report
    Margi R. Desai, Harshita Jaiswal, Mrudangsinh M. Rathod, Sakshi Vijayvargi
    Journal of Pharmaceutical Health Care and Sciences, 2025
    Background Stevens-Johnson Syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) represent life-threatening mucocutaneous reactions, predominantly triggered by medications. This case report presents a rare instance of SJS-TEN overlap in a young Indian female precipitated by the combined use of fluoroquinolone (ofloxacin), cephalosporin (cefixime), and paracetamol—an uncommon drug triad not widely reported in existing literature. The case highlights the importance of early diagnosis, thorough drug history evaluation, and the challenges of managing polypharmacy-induced severe cutaneous adverse reactions (SCARs). Case presentation A 29-year-old Indian female developed widespread dusky purpuric plaques, mucosal erosions (oral, genital, conjunctival, nasal), and bullae six days after local consultation with ofloxacin, cefixime, paracetamol, and other symptomatic agents. She presented to the emergency department with painful vesiculobullous eruptions involving > 10% body surface area, mucosal ulcerations, eye involvement with crusting, and systemic symptoms including fever, vomiting, and urinary discomfort. Laboratory investigations revealed anemia, elevated RDW, and positive ketones in urine. Diagnosis of SJS-TEN overlap was made clinically. The patient was managed with a multi-disciplinary approach involving systemic corticosteroids (IV dexamethasone), hydration, antibiotics (azithromycin), antihistamines, electrolyte balance, topical agents, ophthalmic care, and pain management. The extensive yet individualized treatment regimen reflected a robust pharmacovigilance response to avoid further drug-induced complications. Improvement was noted with complete re-epithelialization and symptomatic resolution over two weeks. Conclusion This case highlights the necessity for clinicians to maintain high suspicion for SCARs in patients presenting with mucocutaneous symptoms and recent drug exposure—even with commonly used medications not frequently associated with SJS-TEN. The unique presentation involving synchronous ocular, nasal, oral, and genital erosions alongside use of a rare drug combination strengthens the need for early recognition, comprehensive clinical assessment, and cautious prescription practices. Individualized treatment and close monitoring are crucial in preventing mortality and minimizing complications. This case underscores the importance of pharmacovigilance and personalized care in managing drug-induced hypersensitivity reactions, especially in resource-limited or polypharmacy scenarios.
  • Green synthesis of copper oxide nanoparticles using genus Inula and evaluation of biological therapeutics and environmental applications
    Abdulaziz Alangari, Mourad A. M. Aboul-Soud, Mohammed S. Alqahtani, Mudassar Shahid, Rabbani Syed, R. Lakshmipathy, Jeevan Kumar Reddy Modigunta, Harshita Jaiswal, Meenakshi Verma
    Nanotechnology Reviews, 2024
    In this research, we produced copper oxide nanoparticles (CuO NPs) using extracts from the entire above-ground portion of plants of genus Inula (Inula graveolens). The synthesis of CuO NPs was verified through various physicochemical analytical methods, including UV–visible, Fourier transform infrared, and transmission electron microscopy. The CuO NPs were found to be around 20 nm in size and spherical in shape. Subsequently, the synthesized compounds were evaluated for their anticancer properties. After treating A549 cells with CuO NPs at concentrations of 15 and 30 μg, we examined their cytotoxicity, lipid peroxidation activity (malondialdehyde level), and antioxidant activity (catalase, superoxide dismutase, and glutathione levels). Additionally, we analyzed the expression of apoptotic marker genes (p53, caspase-3, and caspase-9), cytokine levels (IL-6 and TNF-α), and DNA fragmentation. Our findings demonstrated that CuO NPs enhanced the expression of apoptotic genes, suggesting that phytochemical-derived NPs from Inula extracts induce apoptosis by upregulating tumor suppressor genes and downregulating oncogenes in A549 cells. Furthermore, CuO NPs exhibited higher susceptibility toward B. subtilis and S. aureus compared to ampicillin. Using the response surface methodology, we determined that CuO NPs are effective adsorbents for removing Pb2+ ions from aqueous solutions, making them promising for environmental applications. Overall, our results indicate that CuO NPs have potential as antimicrobial, antioxidant, and anticancer agents and as efficient adsorbents.
  • Early detection and Advanced Targeted Drug Therapies for HER2 positive breast cancer
    Baishakhee Bishoyi, Harshita Jaiswal, Yash Shah, Manoj Dikkatwar, Radhika Bindu
    Research Journal of Pharmacy and Technology, 2023
    HER 2 positive breast cancers are the aggressive subtype of breast cancer which can be treated completely if early detected. According to the National Cancer Institute, localized or stage 1 female breast cancer patients have a relative survival rate of 99% and they can survive for more than 5 years after diagnosis but only 63.1% cases are diagnosed at this stage. In later stages when it's diagnosed, the relative survival rate becomes 29%. The overexpression of HER2 proteins can be detected by early diagnosis and screening through advanced technologies like 3D mammography, Positron Emission Mammography, Molecular breast imaging, Contrast-enhanced mammography. The specific diagnosis can be done by Immunohistochemistry (IHC) and Fluorescence in situ hybridization (FISH) for confirmation of this type of breast cancer. Biomarkers such as Her2 heterogenicity, Her2 expression, Her2 mutations, Hormone receptor (ER positivity, PR positivity), PIK3CA mutation, PTEN, PD-L1, TIL, BRCA, miRNA can be used for detection of breast cancer. With successful early detection, which will be used to diagnose early and start the treatment as early as possible. Recently FDA has approved various individual and combination medications for treating the HER2 positive breast cancer and there are further research studies and clinical trials are going on to get more impactful treatment for providing complete cure of the deadly disease.
  • Antibody conjugated magnetic nanoparticle based colorimetric assay for the detection and quantification of aflatoxin B1 in wheat grains
    R. Kumari, H. Jaiswal, T. Chowdhury, A.K. Ghosh
    World Mycotoxin Journal, 2022
    Aflatoxin B1 (AFB1) is a most potent carcinogenic secondary metabolite produced byAspergillus flavus. As a food safety concern, development of a rapid, cost effective, sensitive and easy to use method for the detection of aflatoxin is of prime requirement. In this study, AFB1 was conjugated with bovine serum albumin (BSA), and AFB1-BSA conjugate was purified by HPLC. Purification was confirmed by UV-Vis spectroscopy, FTIR and MALDI-TOF mass spectrometry. The polyclonal antibody was raised against AFB1-BSA conjugate in rabbit and purified by protein A sepharose and BSA sepharose affinity columns. Iron oxide nanoparticles (MNPs) were synthesised by co-precipitation method and their surface was functionalised with (3-aminopropyl) triethoxysilane (APTES). Size of APTES conjugated MNPs was determined by electron microscopy, and characterised by several biophysical techniques. The purified anti-AFB1 antibody was conjugated with surface functionalised MNPs and the conjugation was confirmed by determining the sizes of free and antibody conjugated MNPs by field emission scanning electron microscope where increase of particle sizes from 10-20 to 40-50 nm was observed due to antibody conjugation. Anti-AFB1 antibody conjugated MNPs were used for capturing AFB1 from the aflatoxin spiked wheat grains with a recovery percentage of more than 80% and used effectively five times. The captured AFB1 was then quantified by a sensitive colorimetric assay where colourless AFB1 was first converted into coumaric acid by NaOH. Subsequently, coumaric acid reacted with 2,6-dibromoquinone-4-chloroimide (DBQC) to a green-coloured indophenol product which was quantified spectrophotometrically. AFB1 contamination as low as 2 μg/kg in wheat grains was detected by the developed technique suggesting its potential application for both qualitative and quantitative analysis of aflatoxins present in feed and food materials.