Isolation and characterization of endophytes from different plants: Effects on growth of Pennisetum typhoides Poonam Gupta, Bhanwar Puniya, Siddhartha Barun, Monika Asthana, Avnish Kumar Biosciences Biotechnology Research Asia, 2014 Studying symbiotic association can provide principles to improve crop yield under harsh conditions. In present study, influence of endophytes was studied on seedling emergence and growth of plumule and radicle of Pennisetum typhoides. The plants were sterilized on surface to prevent contaminations and then macerated in disinfected pestle and mortar to obtain pure colonies of in-habitat bacterial colonies on King’s B media, Nutrient agar and Potato dextrose agar using conventional methods. From 08 plant species, 319 endophytic bacterial cultures were isolated. The isolated endophytes were applied on seeds of Pennisetum typhoides to check the influence on seedling emergence, and growth of radicle and plumule. Out of the 319 cultures, 224 (70.22%) cultures were inhibiting seed germination and growth, while 95 (29.8%) cultures were promoting growth of shoot and/or root of Pennisetum typhoides. Of these 95 growth promoting cultures, 49 (51.6 %) cultures were promoting growth of both shoot and root; 42 (44.2 %) were promoting shoot but inhibiting root growth and 4 (4.21%) were promoting root, but inhibiting shoot growth. Plant species Catharanthus roseus had maximum percentage of inhabitant cultures (58%) that promoted growth of shoot and root. Microbiological characterization of growth promotory cultures revealed that major number of bacteria belongs to Gram positive bacillus (21 cultures) group followed by Gram negative bacillus (16 cultures) group. The mechanism of growth promotion was observed as nitrogen fixation alone (38 cultures), phosphate solubilization along with nitrogen fixation (4 cultures) and other methods (7 cultures). The isolated endophytic strains have the potential to be used as seed inoculants or co-inoculants for improvement in growth, development and yields and thus help in sustainable development in the field of agriculture.
RECENT SCHOLAR PUBLICATIONS
In-Vitro Propagation of Jatropha Curcas, A step towards sustainable Environment S Barun, P Gupta Proceedings of National Conference on Science & Technology for the Moral … , 2017 2017
16SrRNA sequencing of Dye decolorizing bacteria isolated from Soil A Kumar, M Asthana, P Gupta, S Yadav, D Sharma, N Singh, S Kumar Bioinformation 11 (13), 0973-8894 , 2015 2015 Citations: 23
Acid green dye decolorizing bacteria from Yamuna water and textile effluents P Gupta, A Kumar, A Khatri, M Asthana International Journal of Environmental Sciences 5 (3), 482 , 2014 2014 Citations: 2
Physicochemical analysis and microbial diversity of Yamuna water and industrial effluents P Gupta, M Asthana, A Kumar, S Barun International Journal of Applied Sciences and Biotechnology 2 (2), 199-205 , 2014 2014 Citations: 6
Spectrophotometric Analysis of some Reactive Azo Dyes used in Textile Industries P Gupta, S Barun, S Roy Indian Journal of Applied Research 4 (4), 37-39 , 2014 2014 Citations: 5
Isolation and characterization of endophytes from different plants: effects on growth of Pennisetum typhoides P Gupta, B Puniya, S Barun, M Asthana, A Kumar Biosci. Biotechnol. Res. Asia 11 (1), 223-34 , 2014 2014 Citations: 10
Tannery effluents de-colorization efficiency of bacterial isolates from River Yamuna and industrial effluents M Asthana, A Kumar, P Vikrant, P Gupta Int. J. Curr. Microbiol. App. Sci 3 (5), 869-880 , 2014 2014 Citations: 6
Microbial diversity of effluents and adjoining soil of printing and dyeing industries P Gupta, S Barun, S Roy Geobios 40, 92-98 , 2013 2013 Citations: 2
Impact of dyeing and printing industries of Sanganer region of Jaipur, Rajasthan P Gupta, S Barun, S Roy Geobios 39, 246-250 , 2012 2012
MOST CITED SCHOLAR PUBLICATIONS
16SrRNA sequencing of Dye decolorizing bacteria isolated from Soil A Kumar, M Asthana, P Gupta, S Yadav, D Sharma, N Singh, S Kumar Bioinformation 11 (13), 0973-8894 , 2015 2015 Citations: 23
Isolation and characterization of endophytes from different plants: effects on growth of Pennisetum typhoides P Gupta, B Puniya, S Barun, M Asthana, A Kumar Biosci. Biotechnol. Res. Asia 11 (1), 223-34 , 2014 2014 Citations: 10
Physicochemical analysis and microbial diversity of Yamuna water and industrial effluents P Gupta, M Asthana, A Kumar, S Barun International Journal of Applied Sciences and Biotechnology 2 (2), 199-205 , 2014 2014 Citations: 6
Tannery effluents de-colorization efficiency of bacterial isolates from River Yamuna and industrial effluents M Asthana, A Kumar, P Vikrant, P Gupta Int. J. Curr. Microbiol. App. Sci 3 (5), 869-880 , 2014 2014 Citations: 6
Spectrophotometric Analysis of some Reactive Azo Dyes used in Textile Industries P Gupta, S Barun, S Roy Indian Journal of Applied Research 4 (4), 37-39 , 2014 2014 Citations: 5
Acid green dye decolorizing bacteria from Yamuna water and textile effluents P Gupta, A Kumar, A Khatri, M Asthana International Journal of Environmental Sciences 5 (3), 482 , 2014 2014 Citations: 2
Microbial diversity of effluents and adjoining soil of printing and dyeing industries P Gupta, S Barun, S Roy Geobios 40, 92-98 , 2013 2013 Citations: 2
In-Vitro Propagation of Jatropha Curcas, A step towards sustainable Environment S Barun, P Gupta Proceedings of National Conference on Science & Technology for the Moral … , 2017 2017
Impact of dyeing and printing industries of Sanganer region of Jaipur, Rajasthan P Gupta, S Barun, S Roy Geobios 39, 246-250 , 2012 2012