CRISPR Elements and Their Association with Macrolide and Aminoglycoside Resistance Genes in Enterococci Rafael L. do Rêgo, Felipe P.G. Neves, Filipe M. Miranda, Amanda B. da Silva, Amanda S. Cabral, et al. Microbial Drug Resistance, 2025 CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems are common among enterococci and may prevent the acquisition of mobile genetic elements carrying antimicrobial resistance genes. In this study, we correlate the presence of CRISPR with genes associated with macrolide resistance and high-level resistance to aminoglycosides (HLR-A) among 216 Enterococcus faecalis and 82 Enterococcus faecium isolates. We used PCR to detect genes associated with macrolide resistance, HLR-A, and type II-A CRISPR elements. We used two-tailed Fisher's exact test to evaluate correlation between CRISPR and resistance genes. One hundred and seven (35.9%) isolates had at least one HLR-A gene; the prevalent genes were aac(6')-Ie-aph(2″)-Ia and ant(6)-Ia found in 61 (57%) and 46 (43%) isolates, respectively. The macrolide resistance genes erm(A) and erm(B) were found in 116 (38.9%) isolates. Overall, 174 (58.4%) isolates had at least one CRISPR element; the predominant one was CRISPR3-Cas (n = 117; 39.2%). The presence of three genes, two related to HLR-A [aph(2″)-Ic and ant(6)-Ia] and one macrolide resistance gene [erm(B)], was associated with the absence of CRISPR (p < 0.05), mainly in E. faecalis lacking CRISPR3-Cas. We observed the association between the absence of CRISPR and the presence of major aminoglycoside and macrolide resistance determinants, contributing to the understanding of the evolution of resistance in enterococci.
CRISPR-Cas systems in enterococci Amanda Seabra Cabral, Fernanda de Freitas Lacerda, Vitor Luis Macena Leite, Filipe Martire de Miranda, Amanda Beiral da Silva, et al. Brazilian Journal of Microbiology, 2024
Detection of Mycoplasma spp. in free-living seabirds Samara Gomes de Brito, Thomas Salles Dias, Leandro dos Santos Machado, Jenif Braga de Souza, Nathalie Costa da Cunha, et al. Brazilian Journal of Microbiology, 2024
Multidrug-Resistant Staphylococcus aureus Colonizing Pigs and Farm Workers in Rio de Janeiro State, Brazil: Potential Interspecies Transmission of Livestock-Associated MRSA (LA-MRSA) ST398 Joana Talim, Ianick Martins, Cassio Messias, Hellen Sabino, Laura Oliveira, et al. Antibiotics, 2024 Multidrug-resistant (MDR) Staphylococcus aureus has been increasingly isolated from pigs and people in close contact with them, especially livestock-associated methicillin-resistant S. aureus (LA-MRSA). In this cross-sectional study, we investigated S. aureus colonization in pigs and farm workers, their resistance profile, and genetic background to estimate interspecies transmission potential within farms from Rio de Janeiro state, Brazil, between 2014 and 2019. We collected nasal swabs from 230 pigs and 27 workers from 16 and 10 farms, respectively. Five MDR strains were subjected to whole genome sequencing. Fourteen (6.1%) pigs and seven (25.9%) humans were colonized with S. aureus, mostly (64–71%) MDR strains. Resistance to clindamycin, erythromycin, penicillin, and tetracycline was the most common among the pig and human strains investigated. MDR strains shared several resistance genes [blaZ, dfrG, fexA, lsa(E), and tet(M)]. Pig and human strains recovered from the same farm shared the same genetic background and antimicrobial resistance profile. LA-MRSA ST398-SCCmecV-t011 was isolated from pigs in two farms and from a farm worker in one of them, suggesting interspecies transmission. The association between pig management practices and MDR S. aureus colonization might be investigated in additional studies.
Antimicrobial Resistance in Streptococcus pneumoniae before and after the Introduction of Pneumococcal Conjugate Vaccines in Brazil: A Systematic Review Patricia Alice Knupp-Pereira, Amanda Seabra Cabral, Ítalo Moraes Dolores, Amanda Beiral da Silva, Helvécio Cardoso Correa Póvoa, et al. Antibiotics, 2024 Streptococcus pneumoniae causes serious illnesses, such as pneumonia, bacteremia, and meningitis, mainly in immunocompromised individuals and those of extreme ages. Currently, pneumococcal conjugate vaccines (PCVs) are the best allies against pneumococcal diseases. In Brazil, the 10-valent and 13-valent PCVs have been available since 2010, but the threat of antimicrobial resistance persists and has been changing over time. We conducted a systematic review of the literature with works published since 2000, generating a parallel between susceptibility data on isolates recovered from colonization and invasive diseases before and after the implementation of PCVs for routine childhood use in Brazil. This systematic review was based on the Cochrane Handbook for Systematic Reviews of Interventions and Preferred Reporting Items for Systematic Literature Reviews and Meta-Analyses (PRISMA) guidelines. Despite the inclusion of PCVs at a large scale in the national territory, high frequencies of non-susceptibility to important drugs used in pneumococcal diseases are still observed, especially penicillin, as well as increasing resistance to macrolides. However, there are still drugs for which pneumococci have a comprehensive sensitivity profile.
KLEBSIELLA PNEUMONIAE IN MARINE ECOSYSTEM: ESBL AND CARBAPENEMASE ON THE COAST OF RIO DE JANEIRO SR Rocha, MTX dos Santos, PX de Oliveira, AMF Cerqueira, CB Vieira, ... The Brazilian Journal of Infectious Diseases 30, 104689 , 2026 2026
BAYWATCH–SURVEILLANCE OF MDR ESCHERICHIA COLI PRODUCING ESBL IN BEACHES OF NITERÓI/RJ MTX dos Santos, SR Rocha, AL de Andrade Nunes, AMF Cerqueira, ... The Brazilian Journal of Infectious Diseases 30, 104646 , 2026 2026
ENVIRONMENTAL MONITORING OF VANCOMYCIN-RESISTANT ENTEROCOCCI IN BEACHES OF NITERÓI-RJ EK Minervino, MA Wasserman, JP Albuquerque, LMA de Oliveira, ... The Brazilian Journal of Infectious Diseases 30, 104691 , 2026 2026
Antimicrobial resistance and virulence potential of non- faecium and non- faecalis enterococci isolated from canine rectal swabs HS Sabino, LLS Coutinho, CD Marassi, AM Dieckmann, IS Martins, ... Brazilian Journal of Microbiology 56 (3), 2069-2079 , 2025 2025
Antimicrobial resistance and genetic diversity of Escherichia coli isolated from marine bivalves JLC Lima, MMA Jayme, FPG Neves, AC de Freitas-Almeida, MLP Queiroz, ... Brazilian Journal of Microbiology 56 (3), 1585-1593 , 2025 2025
WHO defeating meningitis symposium, 3rd international symposium on Streptococcus agalactiae disease (ISSAD) in Rio de Janeiro, Brazil: State-of-the-art overview of S … LMA Oliveira, N Prasad, R Lynfield, M Ip, S Sanou, FPG Neves, ... Vaccine 52, 126895 , 2025 2025 Citations: 4
CRISPR elements and their association with macrolide and aminoglycoside resistance genes in enterococci RL do Rêgo, FPG Neves, FM Miranda, AB da Silva, AS Cabral, ... Microbial Drug Resistance 31 (3), 75-79 , 2025 2025 Citations: 1
Changes in population genetic structure of serotype 19A Streptococcus pneumoniae after universal childhood use of the 10-valent pneumococcal conjugate vaccine in Brazil JLC Lima, AB da Silva, AS Cabral, FM de Miranda, LD da Silva, ... Vaccine 45, 126588 , 2025 2025 Citations: 1
Long-term predominance in childhood colonization of the multidrug-resistant lineage 6C/ST386 of Streptococcus pneumoniae after universal immunization with the 10-valent … FM Miranda, LD da Silva, LBDP Fortuna, AB Silva, AS Cabral, JLC Lima, ... Vaccine 42 (26), 126414 , 2024 2024
CRISPR-Cas systems in enterococci AS Cabral, FF Lacerda, VLM Leite, FM de Miranda, AB da Silva, ... Brazilian Journal of Microbiology 55 (4), 3945-3957 , 2024 2024 Citations: 5
Detection of Mycoplasma spp. in free-living seabirds SG de Brito, TS Dias, L dos Santos Machado, JB de Souza, NC da Cunha, ... Brazilian Journal of Microbiology 55 (3), 2937-2942 , 2024 2024
Multidrug-Resistant Staphylococcus aureus Colonizing Pigs and Farm Workers in Rio de Janeiro State, Brazil: Potential Interspecies Transmission of Livestock … J Talim, I Martins, C Messias, H Sabino, L Oliveira, T Pinto, J Albuquerque, ... Antibiotics 13 (8), 767 , 2024 2024 Citations: 3
Antimicrobial Resistance in Streptococcus pneumoniae before and after the Introduction of Pneumococcal Conjugate Vaccines in Brazil: A Systematic Review PA Knupp-Pereira, AS Cabral, ÍM Dolores, AB da Silva, HCC Póvoa, ... Antibiotics 13 (1), 66 , 2024 2024 Citations: 13
First Report of a Wastewater Treatment-Adapted Enterococcus faecalis ST21 Harboring vanA Gene in Brazil: First Report of a Wastewater Treatment-Adapted Enterococcus faecalis … BO Farias, KS Montenegro, APA Nascimento, M Magaldi, ... Current Microbiology 80 (9), 313 , 2023 2023 Citations: 7
Prevalence, capsular types, antimicrobial resistance and risk factors associated with pneumococcal carriage among children after long-term 10-valent pneumococcal conjugate … LBDP Fortuna, FM Miranda, IMF Antunes, AB Silva, AS Cabral, ... Vaccine 41 (19), 3111-3118 , 2023 2023 Citations: 13
Drug resistance in maternal and paediatric bacterial and fungal infections: Is COVID-19 changing the landscape FPG Neves, T Mestrovic, TCA Pinto Frontiers in microbiology 14, 1177669 , 2023 2023 Citations: 1
Carriage prevalence, serotype distribution, antimicrobial resistance, pspA typing and pilus islets of Streptococcus pneumoniae isolated from adults living in a Brazilian urban slum AB da Silva, NT Cardoso-Marques, Í de Moraes Dolores, LM Teixeira, ... Vaccine 41 (8), 1431-1437 , 2023 2023 Citations: 4
Mitochondrial DNA and Streptococcus pneumoniae Infection Induction of Immuno-inflammatory Response FPG Neves, D Alessandra, TCA Pinto Mitochondrial DNA and the Immuno-inflammatory Response: New Frontiers to … , 2022 2022
Biofilm formation, interaction and survival within A549 pneumocytes of Klebsiella pneumoniae clinical strains: identification of pulsotypes, multidrug-resistance and genes … BA Nogueira, JGB Olivella, BR Sued-Karam, PMAP Ribeiro Brazilian Journal of Development 8 (8), 55259-55287 , 2022 2022
Streptococcus pneumoniae FPG Neves, TCA Pinto Molecular Typing in Bacterial Infections, Volume I, 139-152 , 2022 2022 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Nasopharyngeal carriage, serotype distribution and antimicrobial resistance of Streptococcus pneumoniae among children from Brazil before the introduction of the … FPG Neves, TCA Pinto, MA Corrêa, R dos Anjos Barreto, ... BMC infectious diseases 13 (1), 318 , 2013 2013 Citations: 63
Multidrug-resistant nontuberculous mycobacteria isolated from cystic fibrosis patients PHC Cândido, LS Nunes, EA Marques, TW Folescu, FS Coelho, ... Journal of clinical microbiology 52 (8), 2990-2997 , 2014 2014 Citations: 59
Antimicrobial resistance profiles and genetic characterisation of macrolide resistant isolates of Streptococcus agalactiae PAM Nakamura, RBB Schuab, FPG Neves, CFA Pereira, GR de Paula, ... Memórias do Instituto Oswaldo Cruz 106, 119-122 , 2011 2011 Citations: 51
Pneumococcal carriage among children after four years of routine 10-valent pneumococcal conjugate vaccine use in Brazil: the emergence of multidrug resistant serotype 6C FPG Neves, NT Cardoso, RE Snyder, MA Marlow, CAA Cardoso, ... Vaccine 35 (21), 2794-2800 , 2017 2017 Citations: 50
Zika: A scourge in urban slums RE Snyder, CE Boone, CAA Cardoso, F Aguiar-Alves, FPG Neves, ... PLoS Neglected Tropical Diseases 11 (3), e0005287 , 2017 2017 Citations: 47
Molecular characterization of quinolone-resistant Neisseria gonorrhoeae isolates from Brazil AA Uehara, ELT Amorin, MF Ferreira, CF Andrade, MBM Clementino, ... Journal of clinical microbiology 49 (12), 4208-4212 , 2011 2011 Citations: 42
Antimicrobial susceptibility patterns, emm type distribution and genetic diversity of Streptococcus pyogenes recovered in Brazil GP Arêas, RBB Schuab, FPG Neves, RR Barros Memórias do Instituto Oswaldo Cruz 109, 935-939 , 2014 2014 Citations: 39
Characteristics related to antimicrobial resistance and biofilm formation of widespread methicillin-resistant Staphylococcus epidermidis ST2 and ST23 lineages in Rio de Janeiro … NLP Iorio, RF Caboclo, MB Azevedo, AG Barcellos, FPG Neves, ... Diagnostic microbiology and infectious disease 72 (1), 32-40 , 2012 2012 Citations: 39
Phenotypic and molecular characterization of optochin-resistant Streptococcus pneumoniae isolates from Brazil, with description of five novel mutations in the atpC gene TCA Pinto, ARV Souza, SECM de Pina, NS Costa, AA Borges Neto, ... Journal of clinical microbiology 51 (10), 3242-3249 , 2013 2013 Citations: 38
Potential of MALDI-TOF MS as an alternative approach for capsular typing Streptococcus pneumoniae isolates TC Pinto, NS Costa, LF Castro, R Rachel L., AC Botelho, FP Neves, ... Scientific Reports 7, 45572 , 2017 2017 Citations: 33
Panton-Valentine leukocidin (PVL) gene carriage among Staphylococcus aureus strains with SCCmec types I, III, IV, and V recovered from cystic fibrosis pediatric patients in Brazil DF Lima, NBV Brazão, TW Folescu, FP Neves, AG Ferreira, EA Santos, ... Diagnostic microbiology and infectious disease 78 (1), 59-62 , 2014 2014 Citations: 33
Evolution of Penicillin Non-susceptibility Among Streptococcus pneumoniae Isolates Recovered From Asymptomatic Carriage and Invasive Disease Over 25 years … TCA Pinto, FPG Neves, ARV Souza, LMA Oliveira, NS Costa, LFS Castro, ... Frontiers in microbiology 10, 486 , 2019 2019 Citations: 32
Population structure of Streptococcus pneumoniae colonizing children before and after universal use of pneumococcal conjugate vaccines in Brazil: emergence … FPG Neves, NT Cardoso, ARV Souza, RE Snyder, MM Marlow, TCA Pinto, ... Journal of Antimicrobial Chemotherapy 73 (5), 1206-1212 , 2018 2018 Citations: 32
Genetic diversity of Ehrlichia canis strains from naturally infected dogs in Rio de Janeiro, Brazil RF Ferreira, AMF Cerqueira, TX Castro, EO Ferreira, FPG Neves, ... Revista Brasileira de Parasitologia Veterinária 23, 301-308 , 2014 2014 Citations: 28
Antimicrobial susceptibility and genetic diversity of Streptococcus agalactiae recovered from newborns and pregnant women in Brazil VC Souza, FCO Kegele, SR Souza, FPG Neves, GR de Paula, RR Barros Scandinavian Journal of Infectious Diseases 45 (10), 780-785 , 2013 2013 Citations: 28
CRISPR elements and their association with antimicrobial resistance and virulence genes among vancomycin-resistant and vancomycin-susceptible enterococci recovered from human … BA Dos Santos, JS de Oliveira, BM Parmanhani-da-Silva, RL Ribeiro, ... Infection, Genetics and Evolution 80, 104183 , 2020 2020 Citations: 26
Emergence of the vanA genotype among Enterococcus gallinarum isolates colonising the intestinal tract of patients in a university hospital in Rio de Janeiro, Brazil FPG Neves, RL Ribeiro, RS Duarte, LM Teixeira, VLC Merquior International journal of antimicrobial agents 33 (3), 211-215 , 2009 2009 Citations: 26
Differences in gram-positive bacterial colonization and antimicrobial resistance among children in a high income inequality setting FPG Neves, MA Marlow, G Rezende-Pereira, MG Pinheiro, ... BMC infectious diseases 19 (1), 478 , 2019 2019 Citations: 25
Bacteraemia associated with a vancomycin-resistant Enterococcus gallinarum strain harbouring both the vanA and vanC1 genes VLC Merquior, FP Gonçalves Neves, RL Ribeiro, RS Duarte, ... Journal of medical microbiology 57 (2), 244-245 , 2008 2008 Citations: 24
Serotypes, antimicrobial resistance and genotypes of Streptococcus pneumoniae associated with infections in cancer patients in Brazil. NFP Cardoso NT, Santos BA, Barbosa AV, Superti SV, Teixeira LM Diagn Microbiol Infect Dis , 2016 2016 Citations: 18