Felipe Astolpho Almeida

Scopus Publications

Unravelling soybean responses to early and late Tetranychus urticae (Acari: Tetranychidae) infestation
K. Ruffatto, L. C. O. da Silva, C. d. O. Neves, S. G. Kuntzler, J. C. de Lima, et al.
Plant Biology, 2024
Soybean is a crucial source of food, protein, and oil worldwide that is facing challenges from biotic stresses. Infestation of Tetranychus urticae Koch (Acari: Tetranychidae) stands out as detrimentally affecting plant growth and grain production. Understanding soybean responses to T. urticae infestation is pivotal for unravelling the dynamics of mite–plant interactions. We evaluated the physiological and molecular responses of soybean plants to mite infestation after 5 and 21 days. We employed visual/microscopy observations of leaf damage, H2O2 accumulation, and lipid peroxidation. Additionally, the impact of mite infestation on shoot length/dry weight, chlorophyll concentration, and development stages was analysed. Proteomic analysis identified differentially abundant proteins (DAPs) after early (5 days) and late (21 days) infestation. Furthermore, GO, KEGG, and protein–protein interaction analyses were performed to understand effects on metabolic pathways. Throughout the analysed period, symptoms of leaf damage, H2O2 accumulation, and lipid peroxidation consistently increased. Mite infestation reduced shoot length/dry weight, chlorophyll concentration, and development stage duration. Proteomics revealed 185 and 266 DAPs after early and late mite infestation, respectively, indicating a complex remodelling of metabolic pathways. Photorespiration, chlorophyll synthesis, amino acid metabolism, and Krebs cycle/energy production were impacted after both early and late infestation. Additionally, specific metabolic pathways were modified only after early or late infestation. This study underscores the detrimental effects of mite infestation on soybean physiology and metabolism. DAPs offer potential in breeding programs for enhanced resistance. Overall, this research highlights the complex nature of soybean response to mite infestation, providing insights for intervention and breeding strategies.
Purification, Structural Characterization, and Anticandidal Activity of a Chitin-Binding Peptide with High Similarity to Hevein and Endochitinase Isolated from Pepper Seeds
Gabriella Rodrigues Gonçalves, Layrana de Azevedo dos Santos, Marciele Souza da Silva, Gabriel Bonan Taveira, Thamyres Marvila da Silva, et al.
Current Microbiology, 2024
Proteomic and histological analysis of in vitro callogenesis in three species of Miconia (Melastomataceae) reveals higher accumulation of proteins related to somatic embryogenesis and embryogenic competence in apomictic species
Juliana K. Ziemmer, Leila N. Vieira, Erika Amano, Felipe A. de Almeida, Vanildo Silveira, et al.
South African Journal of Botany, 2024
Structural and Functional Characterization of New Lipid Transfer Proteins with Chitin-Binding Properties: Insights from Protein Structure Prediction, Molecular Docking, and Antifungal Activity
Gabriella Rodrigues Gonçalves, Marciele Souza da Silva, Layrana Azevedo dos Santos, Thomas Zacarone Afonso Guimarães, Gabriel Bonan Taveira, et al.
Biochemistry, 2024
DNA methylation impacts soybean early development by modulating hormones and metabolic pathways
Fernanda Silva Coelho, Sara Sangi Miranda, Juliana Lopes Moraes, Adriana Silva Hemerly, Helkin Giovani Forero Ballesteros, et al.
Physiologia Plantarum, 2024
Genomic DNA methylation patterns play a crucial role in the developmental processes of plants and mammals. In this study, we aimed to investigate the significant effects of epigenetic mechanisms on the development of soybean seedlings and metabolic pathways. Our analyses show that 5‐azaC‐treatment affects radicle development from two Days After Imbibition (DAI), as well as both shoot and root development. We examined the expression levels of key genes related to DNA methylation and demethylation pathways, such as DRM2, which encodes RNA‐directed DNA Methylation (RdDM) pathway, SAM synthase, responsible for methyl group donation, and ROS1, a DNA demethylase. In treated seedling roots, we observed an increase in DRM2 expression and a decrease in ROS1 expression. Additionally, 5‐azaC treatment altered protein accumulation, indicating epigenetic control over stress response while inhibiting nitrogen assimilation, urea cycle, and glycolysis‐related proteins. Furthermore, it influenced the levels of various phytohormones and metabolites crucial for seedling growth, such as ABA, IAA, ethylene, polyamines (PUT and Cad), and free amino acids, suggesting that epigenetic changes may shape soybean responses to pathogens, abiotic stress, and nutrient absorption. Our results assist in understanding how hypomethylation shapes soybean responses to pathogens, abiotic stress, and nutrient absorption crucial for seedling growth, suggesting that the plant's assimilation of carbon and nitrogen, along with hormone pathways, may be influenced by epigenetic changes.
Decoding the effects of drought stress on popcorn (Zea mays var. everta) flowering combining proteomics and physiological analysis
Katia Fabiane Medeiros Schmitt, Antônio Teixeira do Amaral Junior, Samuel Henrique Kamphorst, Vitor Batista Pinto, Valter Jário de Lima, et al.
Plant Physiology and Biochemistry, 2024
UV-B reduction and excess: Management strategies regarding Coffea sp. crop
Wallace de Paula Bernado, Anne Reis Santos, Ellen Moura Vale, Saulo Pireda, Laísa Zanelato Correia, et al.
Scientia Horticulturae, 2024
Callus induction in Araucaria angustifolia using orthotropic and plagiotropic apexes: proteomic and morphoanatomical aspects
Kathleen Terhaag, Juliana Klostermann Ziemmer, Clarissa Alves Stefanello, Felipe Astolpho de Almeida, Vanildo Silveira, et al.
Plant Cell Tissue and Organ Culture, 2023
Comparative proteomic analysis and antioxidant enzyme activity provide new insights into the embryogenic competence of Guadua chacoensis (Bambusoideae, Poaceae)
Luiza Giacomolli Polesi, Hugo Pacheco de Freitas Fraga, Felipe Astolpho Almeida, Vanildo Silveira, Miguel Pedro Guerra
Journal of Proteomics, 2023
Multiomic Approaches Reveal Hormonal Modulation and Nitrogen Uptake and Assimilation in the Initial Growth of Maize Inoculated with Herbaspirillum seropedicae
Luiz Eduardo Souza da Silva Irineu, Cleiton de Paula Soares, Tatiane Sanches Soares, Felipe Astolpho de Almeida, Fabrício Almeida-Silva, et al.
Plants, 2023
Herbaspirillum seropedicae is an endophytic bacterium that can fix nitrogen and synthesize phytohormones, which can lead to a plant growth-promoting effect when used as a microbial inoculant. Studies focused on mechanisms of action are crucial for a better understanding of the bacteria-plant interaction and optimization of plant growth-promoting response. This work aims to understand the underlined mechanisms responsible for the early stimulatory growth effects of H. seropedicae inoculation in maize. To perform these studies, we combined transcriptomic and proteomic approaches with physiological analysis. The results obtained eight days after inoculation (d.a.i) showed increased root biomass (233 and 253%) and shoot biomass (249 and 264%), respectively, for the fresh and dry mass of maize-inoculated seedlings and increased green content and development. Omics data analysis, before a positive biostimulation phenotype (5 d.a.i.) revealed that inoculation increases N-uptake and N-assimilation machinery through differentially expressed nitrate transporters and amino acid pathways, as well carbon/nitrogen metabolism integration by the tricarboxylic acid cycle and the polyamine pathway. Additionally, phytohormone levels of root and shoot tissues increased in bacterium-inoculated-maize plants, leading to feedback regulation by the ubiquitin-proteasome system. The early biostimulatory effect of H. seropedicae partially results from hormonal modulation coupled with efficient nutrient uptake-assimilation and a boost in primary anabolic metabolism of carbon–nitrogen integrative pathways.
PEG induces maturation of somatic embryos of Passiflora edulis Sims ‘UENF Rio Dourado’ by differential accumulation of proteins and modulation of endogenous contents of free polyamines
Kaliane Zaira Camacho Maximiano Cruz, Felipe Astolpho Almeida, Ellen Moura Vale, Nadia Botini, Rosana Gobbi Vettorazzi, et al.
Plant Cell Tissue and Organ Culture, 2022
Somatic Embryogenesis in Sugarcane (Saccharum spp.)
Felipe Astolpho Almeida, Claudete Santa-Catarina, Vanildo Silveira
Methods in Molecular Biology, 2022
Integrative proteomics and phosphoproteomics reveals phosphorylation networks involved in the maintenance and expression of embryogenic competence in sugarcane callus
Lucas R. Xavier, Felipe A. Almeida, Vitor B. Pinto, Lucas Z. Passamani, Claudete Santa-Catarina, et al.
Journal of Plant Physiology, 2022
Large-scale regeneration of hermaphrodite emblings of Carica papaya L. ‘Golden’ using early molecular sex determination during embryogenic callus multiplication
Lucas Xavier, Felipe Astolpho Almeida, Vitor Batista Pinto, Vanildo Silveira
Plant Cell Tissue and Organ Culture, 2021
Stage-specific protein regulation during somatic embryo development of Carica papaya L. ‘Golden’
Nadia Botini, Felipe Astolpho Almeida, Kaliane Zaira Camacho Maximiano Cruz, Ricardo Souza Reis, Ellen Moura Vale, et al.
Biochimica Et Biophysica Acta Proteins and Proteomics, 2021
Label-Free Quantitative Phosphoproteomics Reveals Signaling Dynamics Involved in Embryogenic Competence Acquisition in Sugarcane
Felipe A. Almeida, Lucas Z. Passamani, Claudete Santa-Catarina, Brian P. Mooney, Jay J. Thelen, et al.
Journal of Proteome Research, 2020
LED lamps enhance somatic embryo maturation in association with the differential accumulation of proteins in the Carica papaya L. ‘Golden’ embryogenic callus
Felipe Astolpho Almeida, Ellen Moura Vale, Ricardo Souza Reis, Claudete Santa-Catarina, Vanildo Silveira
Plant Physiology and Biochemistry, 2019

Felipe Astolpho Almeida

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Scopus Publications