Graduated with a degree in Nursing from the Universidade Paranaense (UNIPAR). Master in Biotechnology, in the line of research in Biotechnology Applied to Health (UFPR). Specialist in Patient Safety and care risk management, Health Audit and Public Health. PhD student of the Postgraduate Program in Health Sciences, in the line of infectious and parasitic diseases (Medical Mycology). CAPES scholarship.
RESEARCH, TEACHING, or OTHER INTERESTS
Multidisciplinary, Applied Microbiology and Biotechnology, Infectious Diseases, Microbiology (medical)
2-ethyl-1-hexanol: a promising molecule against priority fungal pathogens Emilli Karine Marcomini, Pamela Stéphani Tymniak Rezende, Joana Gomes, Cleison Rocha Leite, Kelly Mari Pires de Oliveira, Armando Mateus Pomini, Terezinha Inez Estivalet Svidzinski, Melyssa Negri Journal of Applied Microbiology, 2025 Aim The objective of this study is to evaluate the antifungal activity of 2-ethyl-1-hexanol (2EH), toxicity, including mutagenic potential in vitro and cytotoxicity in vivo, as well as describe physicochemical and pharmacological characteristics of the molecule. Methods and results In vitro susceptibility tests were performed against planktonic cells of Aspergillus fumigatus, Candida spp., and Fusarium spp., priority fungal pathogens. Toxicity was evaluated both in the software, in vitro by the Ames mutagenicity test and in vivo by the larval model. Subsequently, the characterization of the molecule was performed using the SwissADME and Osiris property explorer software. 2EH inhibits the growth of these fungi, with MIC ranging from 0.0520 to 0.0260 g ml−¹ and fungicidal effect. In toxicity, according to software analysis, 2EH does not present mutagenic, tumorigenic, or carcinogenic effects, nor does it cause respiratory and mitochondrial toxicity. In the mutagenicity test, at concentrations lower than 0.1041 g ml−¹, the molecule does not present toxic effects and at MIC concentrations, it was not toxic to Tenebrio molitor (80% survival). 2EH meets the physical, chemical, and pharmacological parameters and has the potential to become a drug. Conclusions 2EH has antifungal action against priority fungal pathogens and at certain concentrations it is not mutagenic, nor does it present toxicity to larvae, therefore, our results suggest that this molecule could be a candidate for the antifungal arsenal.
Silver nanoparticles as antifungal: from formulation to therapy in mycoses Natalia Pecin Bagon, Deisiany Gomes Ferreira, Emilli Karine Marcomini, Melyssa Negri Future Microbiology, 2025 . Comparative data on the mechanism of action of AgNPs in planktonic cells and biofilms are presented, as well as transcriptional analyses in fungi exposed to these nanoparticles. The results demonstrate that formulations containing AgNPs have been applied in various contexts, such as wound dressings, in combination with pharmaceuticals, dental products, and cosmetics, reflecting the growing search for therapeutic alternatives, especially for superficial infections. In experimental models, AgNPs demonstrate applicability in the treatment of dermatomycoses and opportunistic mycoses, promoting healing, low toxicity, and reducing fungal load. They can be used alone or in combination with conventional antifungals. Although still poorly understood, the impact of AgNPs on fungal gene expression suggests modulation of virulence, with potential relevant therapeutic implications. The antifungal activity of AgNPs is effective, although the required concentration varies depending on the target - planktonic cells or biofilms. These data highlight the importance of expanding studies to other fungal pathogens.
Detection of 2-ethyl-1-hexanol and its modulating effect in biofilm of Fusarium oxysporum Flavia Franco Veiga, Emilli Karine Marcomini, Alana Salvador, Lucas Ulisses Rovigatti Chiavelli, Isabella Letícia Esteves Barros, Lidiane Vizioli de Castro, Diego Luis Lucca, Larissa Miwa Kikuchi Ochikubo, Mauro Luciano Baesso, Armando Mateus Pomini, Terezinha Inez Estivalet Svidzinski, Melyssa Negri Molecular Microbiology, 2024 In immunocompetent individuals, Fusarium spp. stands out as the causative agent of onychomycosis, among the non‐dermatophyte molds. Despite evidence indicating that Fusarium oxysporum organizes itself in the form of a biofilm causing onychomycosis, there is little literature on the etiopathogenesis of the biofilm on the nail, specifically the signaling molecules present, known as quorum sensing (QS). Thus, this study detected the presence of a molecule related to QS from the ex vivo biofilm of F. oxysporum on human nail and investigated its effect on preformed biofilm in vitro. The detection and physicochemical characterization of a QS molecule, from the extracellular matrix (ECM), was carried out by Fourier transform infrared (FTIR) spectroscopy with an attenuated total reflectance (ATR) accessory and by headspace gas chromatography coupled to mass spectrometry (GC–MS) analyses. Determination of viable cells, cell activity, total biomass, ECM components and scanning electron microscopy (SEM) were performed to evaluate the influence of the QS molecule on the in vitro biofilm of F. oxysporum. The beginning, in the ex vivo biofilm of F. oxysporum on human nails, the volatile organic compound 2‐ethyl‐1‐hexanol (2EH) was detected as a component of QS. Thereafter in vitro analyses, synthetic 2EH was able to modulate the biofilm by stimulating its filament, increasing total biomass and ECM production in terms of total carbohydrates, but with a reduction in total proteins and nucleic acids. We thus evidence, for the first time, the presence of 2EH in the biofilm of F. oxysporum, developed on the human nail, and the in vitro action of this compound as a QS molecule.
Nursing care systematization: Applicability to primary care Emilli Karine Marcomini, Nanci Verginia Kuster de Paula, Daiane Cortez Raimondi Acta Scientiarum Health Sciences, 2020 Nursing Care Systematization (NCS) is an organized and systematized process that concerns nursing professionals who provide quality assistance, being a mandatory tool in all health centers. Despite this factor, there is a visible resistance from nursing to the operationalization of a systematized care. Thus, the present study aims to analyze the applicability of nursing care systematization and the knowledge that primary care nurses have of this process. This is a descriptive, cross-sectional study with quanti-qualitative approach, conducted with nurses working in primary care teams from a regional health zone in the state of Paraná, Brazil. Out of the 44 participant nurses, only 20.5% claimed to take all steps in the nursing process during their care practice, and 43.2% do not know about the Resolution of the Federal Nursing Council that addresses NCS application. This low NCS applicability is worrisome, since it is related to the quality, resolution and security of care provision. The applicability of the systematization within the assessed teams is quite fragmented and small; besides, their NCS knowledge is insufficient considering that this is such a relevant tool to a nurse’s professional practice. It is possible to notice numerous fragilities and difficulties in the application of NCS to primary care, with highlight to time availability, overworked nurses, and need for training.
RECENT SCHOLAR PUBLICATIONS
Corrigendum to “Fungal quorum-sensing molecules and antiseptics: A promising strategy for biofilm modulation?”[Drug Discov. Today 28 (2023) 103624] EK Marcomini, M Negri Drug Discovery Today, 104641 , 2026 2026
Silver nanoparticles as antifungal: from formulation to therapy in mycoses NP Bagon, D Gomes Ferreira, EK Marcomini, M Negri Future Microbiology 20 (16), 1127-1144 , 2025 2025 Citations: 1
2-ethyl-1-hexanol: a promising molecule against priority fungal pathogens EK Marcomini, PST Rezende, J Gomes, CR Leite, KMP de Oliveira, ... Journal of Applied Microbiology 136 (7), lxaf165 , 2025 2025
Análise das infecções relacionadas ao cateter de diálise. EK Marcomin Saúde. com 20 (4), 3628-3636 , 2024 2024
Detection of 2‐ethyl‐1‐hexanol and its modulating effect in biofilm of Fusarium oxysporum FF Veiga, EK Marcomini, A Salvador, LUR Chiavelli, ILE Barros, ... Molecular Microbiology 122 (5), 630-642 , 2024 2024 Citations: 8
POTENCIAL AÇÃO DE UM MOLÉCULA DE QUORUM SENSING DE FUSARIUM OXYSPORUM EM CANDIDA TROPICALIS EK MARCOMINI, M NEGRI ARQUIVOS DO MUDI Учредители: Universidade Estadual de Maringa 1 (1), 71-74 , 2024 2024
Fungal quorum-sensing molecules and antiseptics: a promising strategy for biofilm modulation? EK Marcomini, M Negri Drug discovery today 28 (7), 103624 , 2023 2023 Citations: 11
Fungal biocompounds as strategy to control infection associated with urinary catheter EK Marcomini, M Negri, J Bernardi-Wenzel Research, Society and Development 11 (17), e21111738880-e21111738880 , 2022 2022
Atuação do enfermeiro no cuidado ao paciente em quadro clínico de sepse: revisão integrativa VL de Moraes, EK Marcomini, APOQ Martins Research, Society and Development 11 (10), e509111033008-e509111033008 , 2022 2022 Citations: 8
Mortalidade infantil em crianças menores de um ano na região noroeste do estado do Paraná EK Marcomini, AGF Frank, NVK Paula, ARV Gerbasi Revista de Saúde Pública do Paraná 5 (2) , 2022 2022
Efeito antimicrobiano e antibiofilme de fungos endofíticos isolados de Handroanthus ochraceus (Bignoniaceae) em cateteres urinários EK Marcomini, J Bernardi-Wenzel 2022
Parto normal: barreiras sob a óptica das parturientes AGF Frank, EK Marcomini, E da Cunha Smaniotto, DG de Moraes, ... Saúde. com 17 (4) , 2021 2021 Citations: 1
Síntese Biológica De Nanopartículas Por Fungos Endofíticos EK Marcomini Revista Multidisciplinar de Educação e Meio Ambiente 2 (3), 34-34 , 2021 2021 Citations: 1
Infecções relacionadas ao uso cateter venoso central: revisão integrativa EK Marcomini, KAD Freitas, NVK de Paula Saúde. com 17 (2) , 2021 2021 Citations: 3
UTILIZAÇÃO DE BIOSSENSORES NO DIAGNÓSTICO DE INFECÇÃO URINÁRIA EK Marcomini Revista Remecs-Revista Multidisciplinar de Estudos Científicos em Saúde, 204-204 , 2020 2020
NANOPARTÍCULAS DE PRATA APLICADA A CATETERES: EFICIÊNCIA COMO AGENTE ANTIMICROBIANO EK Marcomini Revista Remecs-Revista Multidisciplinar de Estudos Científicos em Saúde, 238-238 , 2020 2020
Estratificação de dependência e fragilidades: um olhar sobre os idosos institucionalizados SM Pereira, EK Marcomini, NVK de Paula Revista Kairós-Gerontologia 23 (2), 609-623 , 2020 2020 Citations: 4
Nursing care systematization: applicability to primary care EK Marcomini, NVK de Paula, DC Raimondi Acta Scientiarum. Health Sciences 42, 48465 , 2020 2020 Citations: 8
A Relevância da tecnologia de informação no processo de enfermagem: revisão integrativa E Marcomini, DC Raimondi Saúde. com 15 (2) , 2019 2019 Citations: 3
FATORES DE RISCO EM GESTANTES ATENDIDAS NO SISTEMA ÚNICO DE SAÚDE (SUS), NA REGIÃO NOROESTE DO PARANÁ R Ikino, E Marcomini, AGF Frank, NVK Paula Saúde. com 15 (1) , 2019 2019
MOST CITED SCHOLAR PUBLICATIONS
Influência da simulação realística no ensino e aprendizado da enfermagem EK Marcomini, ES Martins, NV Lopes, NVK de Paula, ... Varia Scientia-Ciências da Saúde 3 (2), 233-240 , 2017 2017 Citations: 13
Fungal quorum-sensing molecules and antiseptics: a promising strategy for biofilm modulation? EK Marcomini, M Negri Drug discovery today 28 (7), 103624 , 2023 2023 Citations: 11
Detection of 2‐ethyl‐1‐hexanol and its modulating effect in biofilm of Fusarium oxysporum FF Veiga, EK Marcomini, A Salvador, LUR Chiavelli, ILE Barros, ... Molecular Microbiology 122 (5), 630-642 , 2024 2024 Citations: 8
Atuação do enfermeiro no cuidado ao paciente em quadro clínico de sepse: revisão integrativa VL de Moraes, EK Marcomini, APOQ Martins Research, Society and Development 11 (10), e509111033008-e509111033008 , 2022 2022 Citations: 8
Nursing care systematization: applicability to primary care EK Marcomini, NVK de Paula, DC Raimondi Acta Scientiarum. Health Sciences 42, 48465 , 2020 2020 Citations: 8
Estratificação de dependência e fragilidades: um olhar sobre os idosos institucionalizados SM Pereira, EK Marcomini, NVK de Paula Revista Kairós-Gerontologia 23 (2), 609-623 , 2020 2020 Citations: 4
Internações em UTI neonatal NVK Paula, A Gerbasi, AP Santos, EK Marcomini, MG Cebrian, ... Espacios 38 (39), 18 , 2017 2017 Citations: 4
Infecções relacionadas ao uso cateter venoso central: revisão integrativa EK Marcomini, KAD Freitas, NVK de Paula Saúde. com 17 (2) , 2021 2021 Citations: 3
A Relevância da tecnologia de informação no processo de enfermagem: revisão integrativa E Marcomini, DC Raimondi Saúde. com 15 (2) , 2019 2019 Citations: 3
Completude das cadernetas de gestantes: realidade da região noroeste do Paraná EK Marcomini, AGF Frank, NN Mizuguchi, ARV Gerbasi, NVK Paul Rev. enferm. atenção saúde, 74-84 , 2019 2019 Citations: 3
Systematization of Nursing Care: Reflections under the theoretical and practical scope EK Marcomini, NVK de Paula Rev Enferm UFPI 8 (2) , 2019 2019 Citations: 2
Silver nanoparticles as antifungal: from formulation to therapy in mycoses NP Bagon, D Gomes Ferreira, EK Marcomini, M Negri Future Microbiology 20 (16), 1127-1144 , 2025 2025 Citations: 1
Parto normal: barreiras sob a óptica das parturientes AGF Frank, EK Marcomini, E da Cunha Smaniotto, DG de Moraes, ... Saúde. com 17 (4) , 2021 2021 Citations: 1
Síntese Biológica De Nanopartículas Por Fungos Endofíticos EK Marcomini Revista Multidisciplinar de Educação e Meio Ambiente 2 (3), 34-34 , 2021 2021 Citations: 1
Sistematização da Assistência de Enfermagem: reflexões sob o âmbito teórico e prático EK Marcomini, NVK Paula Rev. enferm. UFPI, 81-84 , 2019 2019 Citations: 1
Corrigendum to “Fungal quorum-sensing molecules and antiseptics: A promising strategy for biofilm modulation?”[Drug Discov. Today 28 (2023) 103624] EK Marcomini, M Negri Drug Discovery Today, 104641 , 2026 2026
2-ethyl-1-hexanol: a promising molecule against priority fungal pathogens EK Marcomini, PST Rezende, J Gomes, CR Leite, KMP de Oliveira, ... Journal of Applied Microbiology 136 (7), lxaf165 , 2025 2025
Análise das infecções relacionadas ao cateter de diálise. EK Marcomin Saúde. com 20 (4), 3628-3636 , 2024 2024
POTENCIAL AÇÃO DE UM MOLÉCULA DE QUORUM SENSING DE FUSARIUM OXYSPORUM EM CANDIDA TROPICALIS EK MARCOMINI, M NEGRI ARQUIVOS DO MUDI Учредители: Universidade Estadual de Maringa 1 (1), 71-74 , 2024 2024
Fungal biocompounds as strategy to control infection associated with urinary catheter EK Marcomini, M Negri, J Bernardi-Wenzel Research, Society and Development 11 (17), e21111738880-e21111738880 , 2022 2022
