• PhD degree (PhD), thesis entitled “Effect of adult mesenchymal stem cells on bone healing in animal model” Joint degree between Alexandria University, Egypt and University of Tennessee, USA. November 2015
• Master’s degree (MS), Thesis entitled “Evaluation of sedative and analgesic effect of Xylazine, Detomidine and Butorphanol referring to their reversal with some alpha 2 antagonist in donkeys” at Faculty of Veterinary Medicine, Alexandria University, Egypt. October 2011
• B.V.SC, Grade very good with honor, Faculty of Veterinary Medicine, Alexandria University, Egypt. May 2008
• Online Harvard Medical School Courses January 10 to March 24, 2023
HMX Pro Immunology - HMX Pro Genetics
53
Scopus Publications
1314
Scholar Citations
19
Scholar h-index
25
Scholar i10-index
Scopus Publications
Fabrication techniques and biomedical applications of strontium-based nanofibers Esraa Dakrory Mahmoud, Hoda Elkhenany, Tarek M. Bedair Journal of Nanobiotechnology, 2026 Strontium (Sr)-based nanofibers have gained great attention in biomedical and tissue engineering applications due to their unique ability to combine nanoscale structural features with the biological activity of Sr ions (Sr 2+ ). Nanofibers offer a versatile platform to harness these properties owing to their high surface area, tunable porosity, and mechanical strength. The incorporation of Sr 2+ ions further enhances their bio-functionality and offers a cost-effective alternative to growth factor-based strategies. Sr 2+ ions could stimulate the production of growth factors such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), thereby promoting neovascularization, while also enhancing osteogenesis by mimicking calcium’s physiological role, inducing mesenchymal stem cell differentiation, and stimulating extracellular matrix mineralization. This review summarizes recent advances in the fabrication techniques such as electrospinning, assisted-electrospinning, and non-electrospinning, including the design, control composition, morphology, and functionality of Sr-based nanofibers. The mechanisms governing Sr 2+ ions interactions with cells and tissues are discussed, along with in vitro and in vivo biological outcomes. Our bibliometric analysis shows that Sr-based nanofibers have been most extensively investigated in bone tissue engineering, followed by applications in drug delivery and tumor therapy, with fewer studies exploring skin and cartilage regeneration. This review highlights the advantages and disadvantages of every fabrication strategy, discusses biomedical applications of Sr-based nanofibers, and outlines challenges and future directions for their clinical translation. Graphical Abstract
Quail Egg White–Gelatin Nanocomposite Films With rGO–ZnO–Ag Nanocomposite for Antimicrobial and Scar-Reducing Skin Regeneration Ahmed S. Matter, Mohamed Abd Elkodous, Rasha Tawfik, Hossam Tohamy, Howaida Abu‐Ahmed, et al. Journal of Biomedical Materials Research Part B Applied Biomaterials, 2026 Effective wound healing requires dressings that support tissue regeneration while combating infection, particularly from multidrug‐resistant (MDR) pathogens. This study developed and evaluated a multifunctional nanocomposite wound dressing composed of quail egg white (Qeg‐W) and gelatin (GLT) embedded with rGO–ZnO–Ag nanoparticles. Nanocomposite films were prepared by incorporating rGO–ZnO–Ag into Qeg‐W and applied to full‐thickness skin wounds in rats. Wound closure was monitored over 21 days, and tissue samples were collected for histological assessment. Antimicrobial activity against MDR pathogens was evaluated using inhibition zone and MIC assays. The nanocomposite demonstrated robust antimicrobial activity against S. aureus , E. coli , P. aeruginosa , Salmonella enterica , and C. albicans (inhibition zones 14–30 mm; MICs 150–200 μg/mL). All wounds reached complete closure by day 21; however, NP‐treated wounds were associated with significantly reduced scar thickness (~4.5‐fold vs. GLT, p < 0.0001), while GLT–Qeg‐W + NP wounds showed ~2‐fold reduction vs. GLT–Qeg‐W ( p < 0.01). Histopathological evaluation indicated trends toward improved epidermal restoration and regeneration of dermal glands and hair follicles. Overall, GLT–Qeg‐W nanocomposites embedded with rGO–ZnO–Ag nanoparticles exhibited strong standalone antimicrobial activity and were associated with reduced scarring and improved tissue organization. These findings suggest the potential of this sustainable, multifunctional system as a wound dressing, while highlighting the need for further studies to confirm these effects in larger cohorts.
Modeling chondrocyte/synoviocyte inflammation: a comparative approach to recombinant interleukin-10 treatment strategies and their functional differences Hoda A. Elkhenany, Renata L. Linardi, Kyla F. Ortved American Journal of Veterinary Research, 2026 Objective To investigate the impact of recombinant equine IL‑10 (rIL-10) on inflammatory and catabolic responses in stimulated equine chondrocytes and synoviocytes under different treatment timings. Methods Primary chondrocytes and synoviocytes were stimulated with IL‑1β and tumor necrosis factor-α (TNF‑α) and treated with rIL‑10 (10, 20, and 50 ng/mL) under 3 timing models, added 1 hour before stimulation (model 1), 1 hour after stimulation (model 2), or simultaneously with cytokines (model 3). Cultures were maintained for 48 hours. Gene expression was assessed by quantitative real-time PCR, cytokine secretion by multiplex assay, and prostaglandin-E 2 by ELISA. Results rIL‑10 modulated inflammation and catabolic gene expression in a concentration‑ and timing‑dependent manner. In chondrocytes, treatment with 20 ng/mL rIL‑10 reduced IL‑1β, IL‑8, and a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) expression, though 10 ng/mL was more effective in model 2. Treatment with 50 ng/mL rIL-10 increased IL‑6, and 20 ng/mL increased matrix metalloproteinase (MMP)-13. In synoviocytes, rIL‑10 broadly suppressed IL‑1β, IL‑8, MMP-13, and ADAMTS transcripts, though IL‑6 was variably upregulated. At the protein level, rIL‑10 decreased TNF‑α, IL‑6, and IL‑8 secretion in chondrocytes but paradoxically increased IL‑1β under some conditions. In synoviocytes, cytokine secretion changes were less consistent, with modest TNF‑α reduction. rIL‑10 increased prostaglandin-E 2 production in both cell types across all models. Conclusions rIL‑10 modulates equine joint cell inflammation in a concentration‑, cell type‑, and timing‑dependent manner, emphasizing the importance of treatment design in preclinical testing. Clinical Relevance These data support optimizing IL‑10–based biologics and highlight considerations for in vitro screening of anti‑inflammatory treatments for equine osteoarthritis.
PHD-2/HIF-1α axis mediates doxorubicin-induced angiogenesis in SH-SY5Y neuroblastoma microenvironment: a potential survival mechanism Ahmed M. Abou-Shanab, Ola A. Gaser, Noha Galal, Alaa Mohamed, Dina Atta, et al. Scientific Reports, 2025 The response of neuroblastoma (NB) cells to chemotherapeutics and their influence on NB microenvironment remain incompletely understood. Herein, we examined the underlying molecular mechanism via which Doxorubicin, a chemotherapeutic agent used for NB treatment, promotes proangiogenic response in the SH-SY5Y microenvironment. Doxorubicin treatment at 1 µg/ml reduced SH-SY5Y cell proliferation and primed the apoptosis pathway. Unexpectedly, SH-SY5Y cells treated with doxorubicin upregulated their expression of the pro-angiogenic factors, including vascular endothelial growth factor (VEGF), platelets-derived growth factor (PDGF), and matrix metalloprotease-2 (MMP-2) and secretion of nitric oxide. To assess the functional angiogenesis of SH-SY5Y cells pre-treated with doxorubicin, an indirect co-culture system with human umbilical vein endothelial cells (HUVEC) was established. These HUVECs acquired enhanced proliferation, migration capacity, and tube formation capability and exhibited increased nitric oxide (NO) production, in addition to upregulated α-smooth muscle actin expression, suggesting enhanced contractility. In-ovo studies of the neo-angiogenic response of SH-SY5Y pre-treated with doxorubicin further show their promoted neo-angiogenesis as indicated by the generated blood vessels and histological analysis of CD31 expression. Inhibition of PHD-2 could be a potential target for doxorubicin, as indicated by molecular docking, molecular dynamics (MD) simulation, and MM-GBSA calculations, leading to hypoxia-inducible factor-1 alpha (HIF-1α) stabilization. Bioinformatics analyses and enrichment analyses of RNA-seq data revealed activation of Pi3K pathway which is further validated in-vitro. These results provide evidence of the unexpected pro-angiogenic response of SH-SY5Y cells to doxorubicin treatment and suggest the potential use of multi-modal therapeutic regimens for a more comprehensive approach to NB treatment.
The Antifertility Effects of Gossypol–Bovine Serum Albumin Nanoparticles and its Hepatorenal Impact in Male Rats Reham Waheeb, Samah Oda, Hoda Elkhenany, Mayada Marouf, Alshimaa Abd-Elmoneam, et al. Alexandria Journal of Veterinary Sciences, 2025 The current study aimed at evaluating the efficacy of gossypol–bovine serum albumin nanoparticles (Gossy-BSA NPs) as an antifertility compound and to assess its effect on hepatorenal function in rats. For this purpose, eighteen adult male albino rats were allocated into 2 groups: treated rats (n=9), received single bilateral intra-testicular injection of 2mg Gossy-BSA NPs, and control rats (n=9), received normal saline by the same route. Quality of epididymal semen, selected biochemical parameters, testosterone level and the histopathology of testes, epididymis, liver and kidney were estimated at 2-, 4-, and 6-weeks following treatment. Gossy-BSA NPs treatment induced deteriorated semen quality related parameters evidenced by a significant (P < 0.01) reduction in sperm count, motility, and also reduced serum level of testosterone. Serum alanine aminotransferase (ALT) significantly elevated (P < 0.05) at weeks 2 and 6 in treated rats relative to controls. Other parameters such as albumin, bilirubin, urea, creatinine, and CRP showed normal values in treated rats. Histopathological analysis revealed progressive degeneration in seminiferous epithelium and loss of germ cells over the experiment duration. Liver and kidney showed mild to moderate inflammatory changes involving congestion and inflammatory cells infiltration. These findings indicate that Gossy-BSA NPs has antifertility properties with tolerable systemic effects, however further studies are needed to assess their long-term safety and efficacy.
Emerging nanomaterials capable of effectively facilitating osteoblast maturation Hoda Elkhenany Nanomedicine, 2025 Efficient osteoblast maturation is essential for successful bone regeneration, yet achieving this goal remains challenging. This review explores the emerging role of nanomaterials in promoting osteoblast differentiation and bone formation. A literature search was conducted in the Web of Science Core Collection in February 2025, covering publications from 2014 to 2024 and limited to articles and proceedings. Keywords included "nanoparticles" and "osteoblast." Among the most extensively studied nanomaterials were hydroxyapatite, carbon-based, and bioactive glass nanoparticles (NPs). These materials influence osteoblast function through intracellular mechanisms, including enhanced mitochondrial activity, autophagy, and osteoinductive gene expression. Additionally, they modulate the extracellular microenvironment by mimicking the native bone matrix, releasing bioactive ions, and reducing inflammation and oxidative stress. Notably, several NP-based systems have reached clinical application, including Signafuse (a bioactive calcium phosphate composite), nanoLOCK (a nanostructured titanium spinal implant), and Vitoss (a synthetic bone graft of nanocrystalline calcium phosphate). More recently, multimodal NPs that integrate different NP types and combine surface roughness, ion release, and chemical cues offer synergistic effects. These materials provide a dual-function approach, targeting both intracellular processes and the bone microenvironment. Their ability to modulate inflammation, oxidative stress, and cellular signaling underscores their translational potential in regenerative medicine and bone tissue engineering.
Innovative Marine-Sourced Hydroxyapatite, Chitosan, Collagen, and Gelatin for Eco-Friendly Bone and Cartilage Regeneration Hoda Elkhenany, Mariam Waleed Soliman, Dina Atta, Nagwa El‐Badri Journal of Biomedical Materials Research Part A, 2025 In recent years, the exploration of sustainable alternatives in the field of bone tissue engineering has led researchers to focus on marine waste byproducts as a valuable resource. These marine resources, often overlooked remnants of various industries, exhibit a rich composition of hydroxyapatite, collagen, calcium carbonate, and other minerals essential to the complex framework of bone structure. Marine waste by‐products can emit gases such as methane and carbon dioxide, highlighting the urgency to repurpose these materials for innovative tissue regeneration solutions, offering a sustainable approach to address environmental challenges while advancing medical science. Using these discarded materials offers a promising pathway for sustainable development in regenerative medicine. This review investigates the distinctive properties of marine waste byproducts, emphasizing their capacity to be recycled effectively to contribute to the rebuilding of bone and cartilage tissue during regeneration processes. We also highlight the compatibility of these resources with biological materials such as platelet‐rich plasma (PRP), stem cells, exosomes, and natural bioproducts, as well as nanoparticles (NPs) and polymers. By using the natural potential of these resources, we simultaneously address environmental challenges and promote innovative solutions in skeletal tissue engineering, initiating a new era of environmentally green biomedical research.
Successful management of a large feline skin defect using xenogeneic bovine amniotic membrane: a case report A Waleed, H Abu-Ahmed, M Kassem, H Elkhenany BMC Veterinary Research 22 (1), 213 , 2026 2026
Fabrication techniques and biomedical applications of strontium-based nanofibers ED Mahmoud, H Elkhenany, TM Bedair Journal of Nanobiotechnology , 2026 2026
Quail Egg White–Gelatin Nanocomposite Films With rGO–ZnO–Ag Nanocomposite for Antimicrobial and Scar‐Reducing Skin Regeneration AS Matter, MA Elkodous, R Tawfik, H Tohamy, H Abu‐Ahmed, A Korittum, ... Journal of Biomedical Materials Research Part B: Applied Biomaterials 114 (3 … , 2026 2026
Modified Seldinger technique for percutaneous placement of a hemodialysis catheter in a dog SM Skinner, JD Foster, A Fotouh, AM Abd-El Hamed, NK Elbarbary, ... American Journal of Veterinary Research 1 (aop), 1-1 , 2025 2025
Modeling chondrocyte/synoviocyte inflammation: a comparative approach to recombinant interleukin-10 treatment strategies and their functional differences HA Elkhenany, RL Linardi, KF Ortved American Journal of Veterinary Research 1 (aop), 1-12 , 2025 2025
The Antifertility Effects of Gossypol-Bovine Serum Albumin Nanoparticles and its Hepatorenal Impact in Male Rats. R Waheeb, SS Oda, H Elkhenany, M Marouf, AA Abd-Elmoneam, ... Alexandria Journal of Veterinary Sciences 87 , 2025 2025
Equine Bone Marrow–Derived MSCs and Their EVs Exhibit Different Immunomodulatory Effects on Cartilage Explants in an In Vitro Osteoarthritis Model AM Gaesser, JM Cianci, K Even, RL Linardi, G Ruthel, D Barot, ... Cartilage, 19476035251378693 , 2025 2025
Co-culture strategies for muscle–fat tissue development from caprine satellite cells: a step toward sustainable cultivated meat T Elhaddad, E Thabet, MM Essawy, AM Embaby, A Hussein, H Elkhenany Food Research International, 117590 , 2025 2025 Citations: 2
Synergistic Effects of Platelet-Rich Plasma and Amniotic Membrane on Tendon Healing in a Rabbit Model G Abdel-Wahab, A Korittum, H Abou-Ahmed, M El-Kammar, ... Egyptian Journal of Veterinary Sciences, 1-14 , 2025 2025 Citations: 1
PHD-2/HIF-1α axis mediates doxorubicin-induced angiogenesis in SH-SY5Y neuroblastoma microenvironment: a potential survival mechanism AM Abou-Shanab, OA Gaser, N Galal, A Mohamed, D Atta, SS Kamar, ... Scientific Reports 15 (1), 7487 , 2025 2025 Citations: 7
Egyptian Journal of Veterinary Sciences G Abdel-Wahab, A Korittum, H Abou-Ahmed, M El-Kammar, ... 2025
Advances in Perinatal Stem Cells Research and Applications H Elkhenany, Q Wu, A Lotfy Frontiers in Cell and Developmental Biology 13, 1725523 , 2025 2025
Emerging nanomaterials capable of effectively facilitating osteoblast maturation H Elkhenany Nanomedicine 20 (13), 1603–1619 , 2025 2025 Citations: 6
Innovative marine‐sourced hydroxyapatite, Chitosan, collagen, and gelatin for eco‐friendly bone and cartilage regeneration H Elkhenany, MW Soliman, D Atta, N El‐Badri Journal of Biomedical Materials Research Part A 113 (1), e37833 , 2025 2025 Citations: 29
Differential modulation of inflammatory cytokines by recombinant IL-10 in IL-1β and TNF-α ̶ stimulated equine chondrocytes and synoviocytes: impact of washing and timing on … HA Elkhenany, RL Linardi, KF Ortved BMC Veterinary Research 20 (1), 546 , 2024 2024 Citations: 11
Ovarian cauterization versus Cacl2 Injection For "In Situ Ovariectomy" In Female Cats A Kassem, H El-Khenany, H Abu-Ahmed, M El-Kammar, R Abdelwahed Alexandria Journal of Veterinary Sciences 83, 87-87 , 2024 2024
Concentration‐Dependent Effects of MXene Nanocomposite‐Loaded Carboxymethyl Cellulose on Wound Healing S Nasser, MA Elkodous, R Tawfik, H Tohamy, M El‐Kammar, S Nouh, ... Biotechnology Journal 19 (10), e202400448 , 2024 2024 Citations: 3
Comprehensive evaluation of ethanol-preserved amniotic extracts: Exploring antioxidant properties, proliferation enhancement, protective efficacy and regeneration potential in … H Elkhenany, AM Abou-Shanab, S Magdy, SS Kamar, RA Salah, ... Journal of Drug Delivery Science and Technology 100, 106062 , 2024 2024 Citations: 10
Comparison of MXene and SWCNTs effectiveness for osteoblast differentiation: A preliminary study with MG-63 cells MA Elkodous, H Elkhenany, JP Mansell Nano 19 (07), 2450036 , 2024 2024 Citations: 3
Diabetic microenvironment deteriorates the regenerative capacities of adipose mesenchymal stromal cells SM Ahmed, HA Elkhenany, TA Ahmed, NI Ghoneim, MA Elkodous, ... Diabetology & Metabolic Syndrome 16 (1), 131 , 2024 2024 Citations: 8
MOST CITED SCHOLAR PUBLICATIONS
Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering H Elkhenany, L Amelse, A Lafont, S Bourdo, M Caldwell, N Neilsen, ... Journal of Applied Toxicology 35 (4), 367-374 , 2015 2015 Citations: 179
Applications of the amniotic membrane in tissue engineering and regeneration: the hundred-year challenge H Elkhenany, A El-Derby, M Abd Elkodous, RA Salah, A Lotfy, N El-Badri Stem cell research & therapy 13 (1), 8 , 2022 2022 Citations: 177
Tissue regeneration: Impact of sleep on stem cell regenerative capacity H Elkhenany, A AlOkda, A El-Badawy, N El-Badri Life sciences 214, 51-61 , 2018 2018 Citations: 79
Graphene nanoparticles as osteoinductive and osteoconductive platform for stem cell and bone regeneration H Elkhenany, S Bourdo, S Hecht, R Donnell, D Gerard, R Abdelwahed, ... Nanomedicine: Nanotechnology, Biology and Medicine 13 (7), 2117-2126 , 2017 2017 Citations: 66
Comparison of different uncoated and starch-coated superparamagnetic iron oxide nanoparticles: Implications for stem cell tracking H Elkhenany, MA Elkodous, NI Ghoneim, TA Ahmed, SM Ahmed, ... International Journal of Biological Macromolecules , 2020 2020 Citations: 64
Green propolis extract promotes in vitro proliferation, differentiation, and migration of bone marrow stromal cells H Elkhenany, N El-Badri, M Dhar Biomedicine & Pharmacotherapy 115, 108861 , 2019 2019 Citations: 63
Human-induced neural and mesenchymal stem cell therapy combined with a curcumin nanoconjugate as a spinal cord injury treatment P Bonilla, J Hernandez, E Giraldo, MA González-Pérez, A Alastrue-Agudo, ... International journal of molecular sciences 22 (11), 5966 , 2021 2021 Citations: 55
Impact of the source and serial passaging of goat mesenchymal stem cells on osteogenic differentiation potential: implications for bone tissue engineering H Elkhenany, L Amelse, M Caldwell, R Abdelwahed, M Dhar Journal of animal science and biotechnology 7 (1), 16 , 2016 2016 Citations: 55
Curcumin-infused nanostructured lipid carriers: A promising strategy for enhancing skin regeneration and combating microbial infection O Elkhateeb, MEI Badawy, HG Tohamy, H Abou-Ahmed, M El-Kammar, ... BMC Veterinary Research 19 (1), 206 , 2023 2023 Citations: 52
Comparative evaluation of propolis nanostructured lipid carriers and its crude extract for antioxidants, antimicrobial activity, and skin regeneration potential OM Elkhateeb, MEI Badawy, AE Noreldin, HM Abou-Ahmed, ... BMC complementary medicine and therapies 22 (1), 256 , 2022 2022 Citations: 45
Ethnopharmacological evaluation of antioxidant, anti-angiogenic, and anti-inflammatory activity of some traditional medicinal plants used for treatment of cancer in Togo/Africa P Kola, K Metowogo, SN Manjula, G Katawa, H Elkhenany, ... Journal of ethnopharmacology 283, 114673 , 2022 2022 Citations: 39
Efficient tailoring of platinum nanoparticles supported on multiwalled carbon nanotubes for cancer therapy MR Berber, H Elkhenany, IH Hafez, A El-Badawy, M Essawy, N El-Badri Nanomedicine 15 (8), 793-808 , 2020 2020 Citations: 39
Telomerase reverse transcriptase coordinates with the epithelial-to-mesenchymal transition through a feedback loop to define properties of breast cancer stem cells A El-Badawy, NI Ghoneim, MA Nasr, H Elkhenany, TA Ahmed, SM Ahmed, ... Biology open 7 (7), bio034181 , 2018 2018 Citations: 38
Retrospective analysis of local injection site adverse reactions associated with 230 allogenic administrations of bone marrow‐derived mesenchymal stem cells in 164 horses TL Ursini, LL Amelse, HA Elkhenany, A Odoi, JL Carter‐Arnold, HS Adair, ... Equine Veterinary Journal , 2018 2018 Citations: 37
A hyaluronic acid demilune scaffold and polypyrrole-coated fibers carrying embedded human neural precursor cells and curcumin for surface capping of spinal cord injuries H Elkhenany, P Bonilla, E Giraldo, A Alastrue Agudo, MJ Edel, MJ Vicent, ... Biomedicines 9 (12), 1928 , 2021 2021 Citations: 35
Gelatin Loaded Titanium Dioxide and Silver Oxide Nanoparticles: Implication for Skin Tissue Regeneration. N Eldebany, M Abd Elkodous, H Tohamy, R Abdelwahed, M El-Kammar, ... Biological Trace Element Research 199 (10) , 2021 2021 Citations: 31
Innovative marine‐sourced hydroxyapatite, Chitosan, collagen, and gelatin for eco‐friendly bone and cartilage regeneration H Elkhenany, MW Soliman, D Atta, N El‐Badri Journal of Biomedical Materials Research Part A 113 (1), e37833 , 2025 2025 Citations: 29
Bone marrow mesenchymal stem cell-derived tissues are mechanically superior to meniscus cells HA Elkhenany, ARA Szojka, A Mulet-Sierra, Y Liang, M Kunze, X Lan, ... Tissue Engineering Part A 27 (13-14), 914-928 , 2021 2021 Citations: 26
The cross talk between type II diabetic microenvironment and the regenerative capacities of human adipose tissue-derived pericytes: a promising cell therapy TA Ahmed, SM Ahmed, H Elkhenany, MA El-Desouky, S Magdeldin, ... Stem Cell Research & Therapy 15 (1), 36 , 2024 2024 Citations: 21
Stem cell therapy for hepatocellular carcinoma: future perspectives H Elkhenany, A Shekshek, M Abdel-Daim, N El-Badri Advances in Experimental Medicine and Biology, 97-119 , 2019 2019 Citations: 15
