Ekhlas Qanber Jasim
@uobasrah.edu.iq
Pathological Analyses / College of Science
university of Basrah
RESEARCH, TEACHING, or OTHER INTERESTS
Analytical Chemistry, Clinical Biochemistry, Electrochemistry, Organic Chemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Zainab Abdulhussein, Munther Muhammad-Ali, and Ekhlas Jasim
University of Benin
Plant extracts are important in the treatment of many bacterial infections, including henna extracts. Pharmacognosy have become an alternative to traditional medications because of a synergistic effect in combating bacterial infections and no multiple side effects. This investigation examined the antibacterial efficacy of Lawsonia inermis acetone extract against bacteria isolated from urinary tract infections (UTIs) and wounds, including Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli. To isolate the pathogenic bacteria (P. aeruginosa, S. aureus, K. pneumonia, and E. coli), clinical pathogenic samples were obtained. Acetone extract of Lawsonia inermis leaves was produced using Soxhlet extraction and the solution of solid extract was investigated by the cork borer technique which gave an inhibitory zone of 18 to 22 mm against the four species of bacteria. 16 phytocompounds (1a–1p) were identified in the extract using gas chromatography-mass spectrophotometry (GC–MS) peak area percentage (10.66-1.72%). The analysis of phytochemicals using molecular docking simulations of their antibacterial potential revealed binding affinities of – 4.38 to – 7.83 kcal/mol, – 4.67 to – 7.47 kcal/mol, – 5.06 to – 9.07 and – 4.41 to – 7.30 kcal/mol against the dihydropteroate synthase and gyrase B 24kDa proteins of E. coli, and TyrRS and gyrase B proteins of S. aureus, respectively. The extract phytochemicals were subjected to physicochemical parameters evaluation: ADMET predictions. Pharmacokinetic prediction indicates fewer adverse effects. The extract has potential antimicrobial activity, with higher levels of clinical safety based on ADMET predictions.
Munther Abduljaleel Muhammad Ali, Ekhlas Qanber Jasim, and Abeer Issa Mohammed
Manuscript Technomedia LLP
Aim: The objective of this study is synthesis of 1,2,4-triazole derivatives, evaluation there in vitro antimicrobial activity and showing the molecular docking. Materials and Methods: Two series of 5-alkylthio-3-aryl-4-phenyl-1,2,4-triazoles were successfully synthesized. The 1,2,4-triazole thiol was produced via cyclization reaction using the hydrazide compounds (2-hydroxybenzohydrazide and 5-bromofuran-2-carbohydrazide), which were then employed as nucleophilic species to attract various kinds of alkyl halides to synthesis the final compounds. All produced compounds underwent spectroscopic characterization and tested their antibacterial and antifungal activities. With synthetic ligands, molecular docking studies were conducted against the proteins 1AJ0, 1JIJ, and 4ZA5 to identify the crucial interactions underlying antimicrobial activity. Results: UV-visible, FTIR, 1H-NMR and CHNS analysis were confirmed the chemical structures and purity. Initial antibacterial screening results showed that several produced compounds 1e , 1f , 2e , and 2f have good inhibitory effects, whilst some compounds 2e demonstrated high antifungal activity when compared to the control drug fluconazole. Two active substances, 1e and 2e , showed a moderate level of toxicity, with LD 50 values of 3.5 and 2.3 g/kg, respectively. The strongest compounds, 1e , 1f , 2e , and 2f , demonstrated high binding energy antibacterial and antifungal activity, which were supported by docking analysis. Conclusion: Triazole derivatives were synthesized by multi-reaction steps with high yields. Some synthesized triazole derivatives showed promising antibacterial and antifungal activities as compared with parent compounds and standard drugs. The results of antimicrobial activities of compounds were enhanced by good affinity of molecular docking of these compounds with the active site of proteins as compared with standard drugs amoxicillin and fluconazole.
Zahraa Ahmed Mutaeb, Usama H. Ramadhan, and Ekhlas Qanber Jasim
University of Rzeszow
Introduction and aim. Hypertension is a major global health burden and a leading cause of cardiovascular disease and premature death. This study aimed to evaluate the association between serum apelin, inflammatory markers, and protein metabolism parameters in hypertensive patients compared to normotensive controls. This study uniquely explores the interplay between inflammatory and protein metabolism biomarkers using advanced multivariate models in hypertensive adults, a combination not previously examined in this populationMaterial and methods. Two hundred adults aged 35–65 years were divided into hypertensive (n=100) and normotensive (n=100) groups. Serum apelin and C-reactive protein (CRP) levels were measured using enzyme-linked immunosorbent assay, while albumin and total protein were assessed via spectrophotometry. Statistical analyses included t-tests, multiple regression, structural equation modeling (SEM), and Cox regression.Results. Hypertensive patients had significantly higher blood pressure (p<0.001), CRP (7.52±4.21 vs. 1.35±0.51 mg/L; p<0.001), globulin (3.4±1.0 vs. 1.8±0.9 g/dL; p<0.001), and total protein, but lower apelin (2386.2±401.7 vs. 2873.4±572.8 pg/mL; p<0.001) and albumin levels. SEM revealed a direct association between CRP and systolic blood pressure (β=0.45, p<0.001), and a negative association between apelin and systolic pressure (β=−0.20, p=0.03). CRP (HR=1.75, p=0.005) and systolic BP (HR=1.52, p<0.001) were independent predictors of cardiovascular events.Conclusion. The findings suggest that systemic inflammation and dysregulation of serum protein metabolism are significantly associated with hypertension and cardiovascular risk. Apelin may play a protective role by mitigating the impact of inflammation on blood pressure.
Huda Saleh Abood, Ekhlas Qanber Jasim, and Munther Abduljaleel Muhammad-Ali
ARTS Publishing
In this work, four hydrazone Schiff base derivatives N-(2,4-Dinitro-phenyl)-N’-(1H-pyrrol-2-ylmethylene)-hydrazine (1a), N-Benzo [1,3] dioxol-5-ylmethylene-N’-(2,4-dinitro-phenyl)-hydrazine (1b), (E)-5-((2-(2,4-dinitrophenyl)hydrazono)methyl)-2-hydroxybenzoic acid (1c) and (E)-1-(2,4-dinitrophenyl)-2-(2-methoxybenzylidene)hydrazine (1d) were synthesized by reaction of four aldehydes namely pyrrole-2-carboxaldehyde, piperonal, 5-formylsalicylic acid, and o-vanillin with 2,4-dinitrophenyl hydrazine to produce the final compounds 1a, 1b, 1c, and 1d, respectively. These four compounds were investigated as corrosion inhibitors in aqueous mild acidic static solution. FTIR, HNMR, and elemental analysis were used to elucidate the chemical structure of the synthesized inhibitors. Using potential dynamic polarization measurements, these inhibitors’ efficiency in preventing C-steel corrosion in 1.00 M HCl was studied. The results of the experiments revealed that 1×10−3 M is the ideal concentration for 1a, 1b, 1c, and 1d, and that the corresponding inhibition efficiencies for these subunits were 80.70%, 91.30%, 91.34, and 88.80%, respectively. The best corrosion inhibitors were compounds 1b and 1c. Furthermore, studies suggested that these substances are mixed-type inhibitors and that the efficiency of the inhibition is strongly correlated with their quantity. Quantum paraments included Dipole moment, energy band gap (ΔE), value of energy of lowermost unoccupied molecular orbital (ELUMO), and energy of high most occupied molecular orbital (EHOMO) using Molecular Operating Environment MOE, Gaussian, and HyperChem software packages were determined which demonstrated strong agreement between algorithmic and practical findings.
Ekhlas Qanber Jasim
ARTS Publishing
Mesalazine, often referred to as mesalamine or 5-aminosalicylic acid (5-ASA), and its derivatives are some of the first medications to be approved for treating digestive tract inflammations, including ulcerative colitis and mild to moderate Crohn’s disease. Sulfasalazine, discovered in 1938 for therapeutic use, was the first mesalazine derivative. High yields of four different mesalazine derivatives were synthesized, including two Schiff bases and two azo compounds. The present study involved the synthesis of Schiff bases through the reaction of mesalazine with pyrrole-2-carbaldehyde or indole-2-carbaldehyde, resulting in the formation of 5-(((1H-pyrrol-2-yl)methylene)amino)-2-hydroxybenzoic acid (1) or 5-(((1H-indol-2-yl)methylene)amino)-2hydroxybenzoic acid (2), respectively. The synthesis of azo compounds involved the coupling of mesalazine with sulfamethoxazole or pyridoxine, resulting in the formation of 5-amino-2-hydroxy-3-((4-(N-(5-methylisoxazol-3-yl)sulfamoyl)phenyl)diazenyl)benzoic acid (3) or 2-hydroxy-5-((5-hydroxy-3,4-bis(hydroxymethyl)-6-methylpyridin-2-yl)diazenyl)benzoic acid (4), respectively. The identification of the synthesized compounds was carried out using IR and 1H-NMR spectroscopy. Antibacterial assessment of the synthetic compounds was performed in vitro against gram-negative bacteria (such as Escherichia coli and Pseudomonas aeruginosa) and gram-positive bacteria (Staphylococcus aureus). The antibacterial activity studies demonstrated that against Escherichia coli and Staphylococcus aureus, the Schiff base compounds are more active than azo compounds. Compound 1 showed the highest activity, resulting in a 23 mm inhibition zone against E. coli at 1000 ug/ml. In contrast, the antibacterial activity of compound 2 was observed to be 25 mm against S. aureus at the same highest concentration.
Amani Abd Al-Ridha Al-Abdullah, Ekhlas Qanber Jasim, and Munther Abduljaleel Muhammad-Ali
IOP Publishing
Abstract The objective of the current study was to analyze the chemical compositions and antibacterial properties of Laurus nobilis. The bacterial strain was isolated from urine sample of female patients have urinary tract infection in Al-Basrah Teaching Hospital. Two solvents (hot and cold aqueous and ethanol) were used to extract the dried leaves of L. nobilis. While there were differences in the inhibition zones that solvent extracts demonstrated against bacterial pathogens, all of them significantly inhibited pathogens. The diameters of the inhibition zones on Staphylococcus aureus where the alcoholic extract was in the range of 17-29 mm, 22-28 mm for hot water and 12-14 mm for cold aqueous extract. The diameters of the inhibition zones on Klebsiella pneumoniae for alcoholic extract were 18-20 mm, 19-21.5 mm for hot aqueous extract and 12-17 mm for cold water extract. The GC-MS analysis demonstrated the presence of different phytochemical compounds in the extract of Laurus nobilis. A total of 60 compounds were identified, for ethanolic extract, tris (2-methylenecyclopropyl)methanol, (3aS,6aR,9aR,9bS)-3,6,9-trimethylenedecahydroazuleno[4,5-b]furan-2(3H)-one and (3aS,6aR,9aR,9bS)-6-methyl-3,9-dimethylene-3a,4,6a,7,8,9,9a,9b-octahydroazuleno[4,5-b]furan-2(3H)-one were the major compounds with percentage values 9.64%, 8.86% and 7.43%, respectively. For hot water extract, the major three compounds were 5-(hydroxymethyl)furan-2-carbaldehyde 11.64%, 2-methyl-5-nitro-2H-1,2,3-triazol-4-amine 8.39% and tris(2-methylenecyclopropyl)methanol 6.81%. Whereas, for cold water extract, the major compounds were n-Hexadecanoic acid 26.05%, Bis(2-ethylhexyl) phthalate 22.94% and Octadecanoic acid 8.25%. Molecular docking showed that these nine major compounds had an excellent binding affinity -4.25 to -8.56 kcal/mol against S. aureus using protein 1JIJ. The binding affinity of these compounds against K. pneumoniae (protein 6PIB) were in the range - 4.03 to -8.22 kcal/mol.
Hawraa K Deaf, Ekhlas Q Jasim, H. Al-Asadi Rafid, and K M Mohammed
Egypts Presidential Specialized Council for Education and Scientific Research
H.N.K. AL-Salman, Ekhlas Qanber Jasim, and Hussein H. Hussein
NeuroQuantology Journal
Objective: The current study aims to find a suitable, accurate, and faster RP-HPLC technique for the determination of theophylline, which could then be validated in accordance with the International Conference on Harmonization (ICH) guidelines. The Aim of this Study: The aim of this study was to develop an efficient, accurate, and faster RP-HPLC method for determining theophylline, which was then validated using the International Conference on Harmonization (ICH) guidelines. Methods: In the HPLC analysis, the Waters 2695 was used. The drug was isolated better using an Ion Pac zorbax 300-SCX Agilent Column, 5 m, 4.6 250 mm, with a liquid phase of Orthophosphoric acid (0.1 percent Orthophosphoric acid in HPLC acetonitrile and Methanol in the ratio of 50:50 v/v at a flow rate of 1ml/min, with discovery at 280 nm using a PDA detector. Results: Theophylline's preservation time was discovered to be 3.747 0.127 min. In the 5-25 mg/l range, the procedure was found to be linear, with a parallel coefficient (R2) of 0.9998. The LOD and LOQ of the system were determined to be (0.99 and 3) g/ml, respectively. The technique and system precisions were predicted using, and the outcomes were determined as percent RSD principles, which were noticed to be within the strict limitations. Theophylline recovery was detected to be in the 99-100 percent range, confirming the method's precision. Conclusion: Using basic ICH guidelines, the suggested RP-HPLC process was validated. The following methodology can be used successfully and easily for routine diagnostic analysis.
Khawla Salman Abd-Alrassol, H. AL-Salman, E. Jasim and H. Hussein
SynthesisHub Advance Scientific Research
Three quick and sensitive spectrum methods have been suggested to determine Metronidazole. All spectral methods that were used included reducing Metronidazole by mixing zinc powder with hydrochloric acid. The first method (A) included the process of the conjugation of resorcinol reagent with metronidazole and the production of red chromophores, which was then estimated spectrally at a maximum wavelength of 520 nm.
MethodB including the reaction p-chloro benzaldihyde in acidic medium to product yellow color Schiff base has an absorbance at 305 nm. The method C employing a charge transfer reaction with hydroquinone accepter in basic medium to product orange to yellow product absorbed at 335 nm. The best experimental conditions were used by applying the beer's Law for a range of concentrations 2.0-50 μg ml-1, 1.0-35 μg ml-1 and 1.5-30 μg ml-1for methods A, B and C respectively, and the corresponding molar absorptivity values are 1.375×104, 8.672×104, 1.995×104 L mol-1cm-1 with a Correlation coefficient of 0.9996, 0.9998, and 0.9988 and Limits Of Detection (LOD) 0.145 μg ml-1, 0.0776 μg ml-1and 0.110 μg ml-1 and Limits of Quantitation (LOQ) 0.4412 μg ml-1, 0.2352 μg ml-1and 0.3333 μg ml-1 for methods A, B and C Sequentially. The methods were successfully applied to the determination of Metronidazole in pharmaceutical preparation.
E. M. M. Has, Anindita Nayang Safitri and Tiyas Kusumaningrum
This research aimed to chemically synthesize four different sized hydroxyapatite (HA) compound nanoparticles, then coating them with trehalose sugar. After that, a drug release study in an in vitro model was achieved to evaluate the release of ceftaroline fosamil drug from HA different sized coated powders in a simulated body fluid.Calcium nitrate tetrahydrate and diammonium hydrogen phosphate were employed by sol-gel method and wet chemical method to produce the four different sized nanoparticles of hydroxyapatite compound. Identification of the produced HA nanoparticles was implemented using FTIR spectroscopy and X-ray diffraction. Additionally, scanning electron microscopy was utilized to check the morphology and particle size of the synthesized nanoparticles. These nanoparticles were coated by trehalose disaccharide. An in vitro release study was carried out to evaluate the release of ceftaroline fosamil, a low bacterial resistant 5th generation cephalosporin antibiotic, from the four HA coated nanoparticles, at a temperature of 37 OC for 4.5 hours with a time interval of 7.5 minutes, employing simulated body fluid as a releasing medium. The U.V. spectroscopy at I»max of 245.2 nm was used to check the loading amounts and to follow the release of ceftaroline from the synthesized coated HA nanoparicles. The loading percents of ceftaroline on the four identified HA nanoparticles were 59.6% w/w, 53.35% w/w, 38.21% w/w and 32.23% w/w. A percent of 75.84% w/w ceftaroline fosamil was released within 37.5 minutes from the coated hydroxyapatite nanoparticles that was formulated by sol-gel method with sintering for 12 hours. This release remained with a median of 74.95% w/w till the end of the 3rd hour, after that it started to decrease.The release of ceftaroline from HA nanoparticles that was formulated by sol-gel method with sintering for 12 hours, was the fastest to reach the steady state and the highest one during the study time than all other releases, so it is considered effective for futuristic therapeutic uses. The trehalose coating was expected to greatly diminish the hydroxyapatite-ceftaroline ionic interactions, resulting in increased drug release proficiency.
Khawla Salman Abd-Alrassol and Ekhlas Qanber Jasim
IOP Publishing
Abstract Simple and sensitive spectrophotometric method is described for the determination of phenolic compounds in both in pure form and in pharmaceutical preparations. The method is based on the formation of a new ligand from the reaction between 4-aminoantipyrine with phenolic compounds and then reacts with copper (II) to give a colored complex at room temperature. The maximum absorbance of the prepared complexes were measured at 450,500 and 480 nm for pyridoxine, resorcinol and phloroglucinol complexes respectively. Beer’s law was obeyed in the concentration range of 1.5-20, 2.530 and 2.0-25 μg/ml-1, the molar absorptivity values are 2.4778 x 104,1.6740x104 and 1.7001 x 104 L mol−1cm−1, the Sandal sensitivity values are 0.0501x10−3,0.1740x10−3and 0.1228x10−3μg cm−2 for Pyridoxine, resorcinol and phloroglucinol complexes respectively. The correlation coefficients were 0.9999, 0.9998 and 0.9999, the limits of detection(LOD) were 0.47793, 0.15125 and 0.01434μg ml−1, the limits of quantification were (LOQ) 1.4482, 0.45833 and 0.04347 for pyridoxine, resorcinol and phloroglucinol complexes respectively. The stoichiometry of the complexes formed (1:2) was determined by Job’s continuous variations method and molar ratio method. Furthermore the stability constant (K) and Gibbs free energy ΔG) for the complexes were also calculated. The proposed methods were applied successfully for the determination of phenolic compounds in commercial tablets.
Hawraa Kareem Dhaif, Ekhlas Q . Jasim, Zena A. Muhajjar, and Ashwaq A. Shanta
Mediterranean Journal of Chemistry
The Weight loss was employed to investigate the impact of triazole on mild-steel dissolution in 0.5 M HCl solution. The inhibitor’s inhibition efficiency was seen to increase with concentration yielding (81.61%,82.61%,88.29%,91.64%,94.32%) of (T9, T8, T6, T4, T1) at concentration 1×10-4 M HCl, at a temperature of 25°C for 240 min. At a temperature range from 25–45°C, we studied the temperature impact on the corrosion behavior, wherein the results demonstrated decrease in inhibition efficiency with rising in temperature to achieve (61.7%, 52.26%, 63.1%, 72.11%, 75.77 %) of (T9, T8, T6, T4 and T1) at a concentration of 1×10-4 M, at a temperature 45°C for 240 min. A study was also performed regarding the impact of temperature on the corrosion rate in the presence and absence of triazole. The activation energy and Kinetic parameters were calculated and discussed. Polarization curves revealed that the studied inhibitors represent a mixed – type inhibitors. Adsorption of inhibitors was found to obey Langmuir isotherm and was isotherm physisorption type.
Suhail H. Derawey, Mazin N. Mosa, Ekhlas Qanber Jasim, and Rawaa M. O. Hraishawi
GP Innovations Pvt. Ltd.
The present work includes synthesis and characterization of some novel 1,3,4-oxadiazole derivatives and the evaluation of the antibacterial activity of the synthesized compound against pathogenic isolated gram-negative and gram-positive bacteria. The activity result showed that some compound exhibited efficient effect against these bacteria. Some compounds had significant inhibition zone against Escherichia coli, pseudomonas aeruginosa and Staphylococcus aureus. The compound OXD3 and OXD4 gave inhibition zone against resistant pseudomonas aeruginosa while stander drug cefepime doesn’t give an activity.
Ekhlas Qanber Jasim, M.A. Munther Abduljaleel, and Rajaa Hussein Fayadh
Asian Journal of Chemistry
five 1,3,4-thiadiazole compounds were synthesized. The prepared compounds were identified by CHNS analysis, FT-IR and 1 H NMR spectroscopy. The corrosion rates in the presence of thiadiazole as a steel corrosion inhibitor in the cooling water system which taken from South Fertilizer Company, Basra, Iraq were measured by the weight loss method and potentiodynamic polarization measurements. The weight loss method was studied in different times (1-5 h) and in 303 to 333 K temperature range. Results obtained revealed that thiadiazole compounds performed as a corrosion inhibitor for mild steel in this medium and its efficiency attains to 86.55 % at 5 × 10 -3 M at 303 K and by potentiodynamic polarization measurements its efficiency attains to 85.35 % in the same conditions. The apparent activation energies, enthalpies and entropies of the dissolution process and the free energies were determined and discussed.