Eman Mahmoud Abu El-Naga

Scopus Publications

Foetal Mummification in Pregnant Dairy Cows Induces Variant Changes on the Hormonal Profile, Biochemical Parameters and Mineral Profile of the Dam
Yahia A. Amin, Obeid Shanab, Ibrahim S. Zahran, Foad Farrag, Mariam A. Fawy, et al.
Veterinary Medicine and Science, 2025
Background and aimFoetal mummification does not cause loss by losing foetus only but the harm extends to the mother dam. This is the first study that investigates the changes that occur in the dam's body due to foetal mummification through a comprehensive investigation of the hormonal, biochemical, liver, kidney and mineral profiles of the dam. This study represents a model to investigate the pathogenesis of such clinically severe cases.Materials and methodsThis study involved 40 cows with foetal mummification. The dams were divided into 2 groups (20 for each): Group 1 (cows carrying normal foetuses [CNF]) and Group 2 (cows carrying mummified foetuses [CMF]). Blood samples were collected for evaluation of hormonal profile, biochemical profile, kidney profile, liver profile and mineral profile.ResultsResults showed that progesterone (P4) and oestradiol (E2) concentrations were not significantly changed in the CMF group compared to the CNF group, whereas total triiodothyronine (T3) and total thyroxin (T4) showed a significant reduction in mummified cases than in CNF group. In the CMF group, parameters such as glucose and albumin were significantly lower compared to the normal pregnant animals. In contrast, triglycerides, cholesterol, globulin and total protein (TP) were significantly higher. Kidney and liver profiles showed a significant increase in urea, aspartate aminotransferase (AST), alkaline phosphatase (ALP) and bilirubin, a significant decrease in alanine aminotransferase (ALT) and non‐significant differences in creatinine and gamma‐glutamyl transpeptidase (GGT) in CMF compared with normal pregnant cows. Mineral profiles showed a significant decrease in calcium and phosphorus, a significant increase in iron and potassium and a non‐significant increase in magnesium, sodium and chloride in the mummified group compared to the normal pregnancy group.ConclusionCows with mummified foetuses (MF) maintained variant biological changes in the body. These findings can be used as an indicator for cow health and as a diagnostic tool to avoid pregnancy disorders that occur during the late pregnancy.
Effect of histidine and L-Tyrosine supplementation in maturation medium on in-vitro developmental outcomes of buffalo oocytes
Eman M. Abu El-Naga, Montaser Elsayed Ali, Ramya A. Sindi, Hassan A. Hussein
BMC Veterinary Research, 2024
Cleavage and in vitro cultivation rates monitoring in culture media supplemented with energy sources, non-essential amino acids, and antioxidants in the buffalo embryos
Eman M Abu El-Naga, Montaser E. Ali, Rawda H. Ali, Heba F. Hozyen, Hassan A. Hussein
BMC Veterinary Research, 2024
The study was designed to monitor the cleavage rate (CR) and in-vitro cultivation rate (IVC) after addition of energy sources, non-essential amino acids, and antioxidants to the Synthetic oviductal fluid (SOF) and FertiCult. After in-vitro maturation and in-vitro fertilization, presumptive zygotes were cultured in one of two culture media: FertiCult media and SOF medium, supplemented with pyruvate, glucose, and sodium lactate as energy sources, as well as 10, 20, 250, 500, and 750 mg non-essential amino acids, and antioxidants. All stages of cleavage rate (CR), and in-vitro cultivation rate (IVC) of embryonic development including morula stage (MOR) and blastocyst (BLAS) have been assessed. The findings revealed that there were no significant differences in the CR between the control and other treated groups with sources of energy when added to SOF media (P > 0.05), while there were significant differences (P < 0.05) in the IVC of embryonic development between groups (The percentages of MOR stage in the control, pyruvate, glucose and mixture of source of energy (MIX) were at 50%, 62.5%, 60%, and 63.6%, respectively). The highest percentage of the BLAS was recorded after SOF supplementation with glucose (40%). Similarly, there were no significant differences (P > 0.05) in the CR between control and FertiCult supplemented with sources of energy, while the IVC stages increased significantly (P < 0.05) in the FertiCult media supplemented with glucose, pyruvate, sodium lactate, and MIX. The percentages of the MOR stage in the control, pyruvate, glucose and mix media were at 50%, 55.6%, 55.6%, 54.5%, 57.1% respectively. The lowest percentage of the BLAS was recorded after FertiCult supplementation with pyruvate (11.1%). Replenishing the SOF maturation media with 20 mg of non-essential amino acids significantly (P < 0.05) enhanced the MOR stage (100%). There was also an improvement in the development of BLAS stage, where it reached 31.2% and 47.4% in the SOF maturation media supplemented with 10, and 750 mg non-essential amino acids, respectively. There were no significant differences (P > 0.05) in neither CR nor IVC between control and FertiCult supplemented with antioxidants. There were significant differences (P < 0.05) in the MOR stages (control, 42.9% & treated, 57.9%) and BLAS stages (control, 21.4% & treated, 42.1%) in antioxidant supplemented SOF maturation media compared to control. In conclusion, supplementation of SOF cultivation medium with energy sources, 20 mg of non-essential amino acids and antioxidant addition may improve the cleavage rate (CR) and in vitro cultivation rate (IVC) of buffalos’ embryonic development.
Effect of different post-partum therapeutic protocols with intrauterine oxytetracycline, oxytocin and/or GnRH injection in post-kidding goats on oxytetracyclines residues in goat milk and postpartum ovarian resumption with referring to clinical and haematological pictures
Asem M. Zakaria, Taher Al-Daek, Enas Elmeligy, Ragab H. Mohamed, Eman M. Abu El-Naga, et al.
BMC Veterinary Research, 2023
Background The post-parturient period in goat had marked changes in an animal’s endocrine and metabolic status as well as by reduction in feed intake when the nutrient demand for impending lactogenesis was increasing. The current study aimed to monitor the residues of oxytetracycline in Baladi goat milk and their hazards on public health as well as the time required until complete disappearance of this medicament from milk through following up periods included 0, 12, 24, 36, 48, 60, 72, 84, 96 and 120 h in post-kidding goat following intrauterine application of oxytetracycline. The study also compared between the efficacy of oxytetracycline only, oxytetracycline with oxytocin, or oxytetracycline with GnRH, through monitoring the clinical findings and haematological pictures at days 0, 5 and 7 post-partum as well as studying the changes in numbers and size of follicles at days 15, 30 and 45 postpartum after different treatments strategies in different groups i.e. Control healthy goat (Contgr), Oxytetracycline treated goat (Oxytetgr), Oxytetracycline-oxytocin treated goat (Oxytet-Oxytogr) and Oxytetracycline-GnRH treated goat (Oxytet-GnRHgr). The study was carried out on clinically healthy Baladi goats (n = 40) that gave birth recently. They were divided into 4 equal groups (n = 10 goats for each); Contgr which received no medication after birth, Oxytetgr which administrated oxytetracycline tablets intrauterine at day of birth, Oxytet-Oxytogr which treated by oxytetracycline tablets intrauterine at day of birth followed by oxytocin injection at 3rd day after birth, and Oxytet-GnRHgr which treated by oxytetracycline tablets intrauterine at day of birth followed by GNRH injection at 3rd day after birth. Results and Conclusions The study concluded the highest oxytetracyclines residues in goats’ milk were reported after 36 h following intrauterine oxytetracycline application where complete disappearance of oxytetracyclines residues in goats’ milk required 120 h elapsed after intrauterine oxytetracycline application in which the goats milk became safe for human consumption. The study also reported powerful influence of the applied variable therapeutic regimens on post-partum ovarian resumption through clear significant variations in numbers and sizes of follicles either between different goats’ groups within the same day, or between days 15, 30 and 45 post-partum within each independent goat group.
DNA Polymorphisms and mRNA Levels of Immune Biomarkers as Candidates for Inflammatory Postpartum Disorders Susceptibility in Italian Buffaloes
Ahmed Ateya, Fatmah A. Safhi, Huda El-Emam, Muath Q. Al-Ghadi, Mohamed Abdo, et al.
Veterinary Sciences, 2023
The immunological genes that may interact with inflammatory postpartum diseases in Italian buffaloes were examined in this study. A total number of 120 female Italian buffaloes (60 normal and 60 with inflammatory reproductive diseases) were employed. Each buffalo’s jugular vein was pierced to get five milliliters of blood. To obtain whole blood and extract DNA and RNA, the blood was placed within tubes containing sodium fluoride or EDTA anticoagulants. The immunological (IKBKG, LGALS, IL1B, CCL2, RANTES, MASP2, HMGB1, and S-LZ) genes’ nucleotide sequence differences between healthy buffaloes and buffaloes affected by inflammatory reproductive diseases were found by employing PCR-DNA sequencing. According to Fisher’s exact test (p ˂ 0.01), there were noticeably different probabilities of all major nucleotide changes spreading among buffalo groups with and without reproductive problems. Buffaloes were significantly more likely to express the examined genes when they had inflammatory reproductive diseases. The outcomes might support the significance of these markers’ nucleotide variations and gene expression patterns as indicators of the prevalence of inflammatory reproductive disorders and provide a workable buffalo management policy.
Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle
Mona Al-Sharif, Mohamed Abdo, Omnia El Shabrawy, Eman M. Abu El-Naga, Liana Fericean, et al.
Veterinary Sciences, 2023
This study looked at genetic polymorphisms and transcript levels of immune, antioxidant, and erythritol-related markers for postparturient endometritis prediction and tracking in Holstein dairy cows. One hundred and thirty female dairy cows (65 endometritis affected and 65 apparently healthy) were used. Nucleotide sequence variations between healthy and endometritis-affected cows were revealed using PCR-DNA sequencing for immune (TLR4, TLR7, TNF-α, IL10, NCF4, and LITAF), antioxidant (ATOX1, GST, and OXSR1), and erythritol-related (TKT, RPIA, and AMPD1) genes. Chi-square investigation exposed a noteworthy variance amongst cow groups with and without endometritis in likelihood of dispersal of all distinguished nucleotide variants (p < 0.05). The IL10, ATOX1, and GST genes were expressed at substantially lower levels in endometritis-affected cows. Gene expression levels were considerably higher in endometritis-affected cows than in resistant ones for the genes TLR4, TLR7, TNF-α, NCF4, LITAF, OXSR1, TKT, RPIA, and AMPD1. The sort of marker and vulnerability or resistance to endometritis had a significant impact on the transcript levels of the studied indicators. The outcomes might confirm the importance of nucleotide variants along with gene expression patterns as markers of postparturient endometritis susceptibility/resistance and provide a workable control plan for Holstein dairy cows.
Changes In The Fetal Fluids Compositions During Dystocia Of Dairy Buffaloes
Yahia A. Amin, Enas A. Noseer, Eman M. Abu El-Naga
Advances in Animal and Veterinary Sciences, 2020
| Fetal fluids have different vital functions to sustain pregnancy and healthy parturition. Besides a deep understanding of fetal metabolism, diagnosis of pathological conditions during gestation and studying the composition of fetal fluids may provide information about fetal requirements for growth and maturation. With this background, the study was carried out in ninety pregnant buffaloes divided into two equal groups of eutocia and dystocia. Hormonal (cortisol and estradiol) and biochemical profile (cholesterol, glucose, total protein, creatinine, GGT, calcium, sodium, and potassium) of fetal fluid was measured for each of the buffalo. The cholesterol concentration in the case of dystocia had no significant difference from that found in the case of eutocia (P= 0.09). The level of cortisol, glucose, creatinine, sodium, calcium, and GGT was significantly higher in buffaloes with dystocia (P < 0.05). On the other hand, an increased estradiol concentration was observed in buffaloes with eutocia (P < 0.05). Cholesterol and potassium concentration did not vary significantly in both groups of buffaloes (P ˃ 0.05). This study concluded that dystocia causes a significant change in hormonal and biochemical profiles of the fetal fluids. Hence, in addition to the fetus in the prenatal and postnatal stages of its life, an analysis of fetal fluids indicates the health status of the dam.
Synchronization with controlled internal drug release (CIDR) and prostaglandin F2α (PGF2α) influences oxidant/antioxidant biomarkers and mineral profile in summer-stressed anoestrous buffalo (Bubalus bubalis)
Yahia A. Amin, Eman M. Abu El- Naga, Enas A. Noseer, Samer S. Fouad, Rana A. Ali
Theriogenology, 2019
During the summer season, high ambient temperature in tropical and subtropical countries exposes buffaloes to oxidative stress that have antigonadotropic and antisteroidogenic effects. Uses of hormonal therapy can improve the state of ovarian inactivity caused by heat stress and cause anoestrous buffaloes to come into oestrus and successfully achieve pregnancy. Therefore, this study was designed to detect the role of oxidative stress in production of the anoestrous state in summer heat stressed buffaloes and the effects of treatment by Controlled internal drug release (CIDR) in solving of this problem. Also it monitored the changes in Oxidant/antioxidant biomarkers and mineral profile before and after the treatment. Forty buffaloes with no signs of oestrus for more than 120 days after calving were selected. The animals were divided into two groups: the first group (group I, n = 25) was subjected to treatment with CIDR, while the second group (group II) received no treatment and was considered the control group (n = 25). Blood samples were collected before treatment, after treatment and after 45 days of oestrus. The serum level of TAC, MDA, NO, ascorbic acid (vitamin C), P, Cu and Zn were measured. The results showed that 80% of treated buffaloes came into oestrus. The conception rate was 75%. TAC concentrations were significantly higher in group I than in group II. There were significant decreases in the mean values of MDA, NO and ascorbic acid in the buffaloes in oestrus, but these values increased when the buffaloes became pregnant. In contrast there were no significant differences in the mean values of MDA, NO or ascorbic acid in the buffaloes that came into oestrus but failed to conceive. The mean serum P, Cu and Zn values were significantly increased (P < 0.05) in the buffaloes that came into oestrus compared to the control animals. The levels of P and Zn significantly increased when the buffaloes became pregnant and remained unchanged when they failed to conceive. In conclusion, known physiological and pathological variations in the oxidant/antioxidant parameters and mineral profile of summer anoestrous buffaloes may help to understand this problem of infertility.
Effect of media, sera and hormones on in vitro maturation and fertilization of water buffalos (Bubalus bubalis)
A.M. Hammam, C.S. Whisnant, A. Elias, S.M. Zaabel, A.O. Hegab, et al.
Journal of Animal and Veterinary Advances, 2010

RECENT SCHOLAR PUBLICATIONS

L-Carnitine-Mediated Antioxidant Defence in Buffalo Oocytes: A Novel Approach for Improving In Vitro Maturation and Embryo Developmental Competence
EM Abu El-Naga, DA Soliman, MK Hussein, RH Mohamed, A Monir, ...
Egyptian Journal of Veterinary Sciences, 1-9 , 2025
2025.0
Foetal Mummification in Pregnant Dairy Cows Induces Variant Changes on the Hormonal Profile, Biochemical Parameters and Mineral Profile of the Dam
YA Amin, O Shanab, IS Zahran, F Farrag, MA Fawy, M Shukry, ...
Veterinary Medicine and Science 11 (3), e70304 , 2025
2025.0
Citations: 1
Cleavage and in vitro cultivation rates monitoring in culture media supplemented with energy sources, non-essential amino acids, and antioxidants in the buffalo embryos
EM Abu El-Naga, ME Ali, RH Ali, HF Hozyen, HA Hussein
BMC Veterinary Research 20 (1), 521 , 2024
2024.0
Citations: 2
Effect of histidine and L-Tyrosine supplementation in maturation medium on in-vitro developmental outcomes of buffalo oocytes
EMA El-Naga, ME Ali, RA Sindi, HA Hussein
BMC Veterinary Research 20 (1), 414 , 2024
2024.0
Citations: 3
Effect of histidine and L-Tyrosine supplementation in maturation medium on in-vitro developmental outcomes of buffalo oocytes
EMAEN Eman M. Abu El-Naga, Montaser Elsayed Ali, Ramya A. Sindi, Hassan A ...
BMC veterinary , 2024
2024.0
DNA polymorphisms and MRNA levels of immune biomarkers as candidates for inflammatory postpartum disorders susceptibility in Italian buffaloes
A Ateya, FA Safhi, H El-Emam, MQ Al-Ghadi, M Abdo, L Fericean, R Olga, ...
Veterinary Sciences 10 (9), 573 , 2023
2023.0
Citations: 4
Effect of different post-partum therapeutic protocols with intrauterine oxytetracycline, oxytocin and/or GnRH injection in post-kidding goats on oxytetracyclines residues in …
AM Zakaria, T Al-Daek, E Elmeligy, RH Mohamed, EMA El-Naga, ...
BMC veterinary research 19 (1), 139 , 2023
2023.0
Citations: 7
addition of histidine and L-tyrosine to maturation and culture media on IVM, IVF and IVC of buffalo oocytes and embryos
EMA El-Naga, ME Ali, RA Sindi, HA Hussein
2023.0
Investigating polymorphisms and expression profile of immune, antioxidant, and erythritol-related genes for limiting postparturient endometritis in Holstein cattle
M Al-Sharif, M Abdo, OE Shabrawy, EMA El-Naga, L Fericean, ...
Veterinary Sciences 10 (6), 370 , 2023
2023.0
Citations: 13
Changes in the fetal fluids compositions during dystocia of dairy buffaloes
YA Amin, EA Noseer, EMA El-Naga
Adv. Anim. Vet. Sci 8 (7), 728-734 , 2020
2020.0
Citations: 6
Synchronization with controlled internal drug release (CIDR) and prostaglandin F2α (PGF2α) influences oxidant/antioxidant biomarkers and mineral profile in summer-stressed …
YA Amin, EMA El-Naga, EA Noseer, SS Fouad, RA Ali
Theriogenology 134, 34-41 , 2019
2019.0
Citations: 19
Soybean lecithin–based extender improves Damascus goat sperm cryopreservation and fertilizing potential following artificial insemination
M Fathi, R Zaher, D Ragab, I Gamal, A Mohamed, E Abu-El Naga, M Badr
Asian Pacific Journal of Reproduction 8 (4), 174-180 , 2019
2019.0
Citations: 13
Diagnosis of Brucellosis in Recently Aborted Ewes Using Serological Tests and Polymerase Chain Reaction
Hassan Ali Hussein, Ragab Hassan Mohamed, Aml Mohammed Abdel-Ra`ouf, Eman ...
Journal of Applied Sciences 19 (2), 77-81 , 2019
2019.0
Citations: 13
Improving in vitro maturation and cleavage rates of buffalo oocytes
AO Hegab, AE Montasser, AM Hammam, EMAA El-Naga, SM Zaabel
Animal Reproduction (AR) 6 (2), 416-421 , 2018
2018.0
Citations: 46
Effect of Enrichment of
SM Zaabel, EM Abu-El Naga, H El-Sheikh Ali, AZ Balboula, ...
Vitro , 2017
2017.0
Effect of media, sera and hormones on in vitro maturation and fertilization of water buffalos ( Bubalus bubalis ).
AM Hammam, CS Whisnant, A Elias, SM Zaabel, AO Hegab, ...
2010.0
Citations: 39
Mummification of the fetus in pregnant cows induces variant biological changes in the body
Y Amin, O Shanab, IS Zahran, F Farrag, MA Fawy, BD Ioan, L Fericean, ...
Frontiers in Veterinary Science 11, 1463388 , 0
Effect of sperm motility enhancers on in vitro fertilization and embryo development of buffalo oocytes
EM Abu-El Naga, HES Ali, MR Badr, AM El, SMZ Desouky

MOST CITED SCHOLAR PUBLICATIONS

Improving in vitro maturation and cleavage rates of buffalo oocytes
AO Hegab, AE Montasser, AM Hammam, EMAA El-Naga, SM Zaabel
Animal Reproduction (AR) 6 (2), 416-421 , 2018
2018.0
Citations: 46
Effect of media, sera and hormones on in vitro maturation and fertilization of water buffalos ( Bubalus bubalis ).
AM Hammam, CS Whisnant, A Elias, SM Zaabel, AO Hegab, ...
2010.0
Citations: 39
Synchronization with controlled internal drug release (CIDR) and prostaglandin F2α (PGF2α) influences oxidant/antioxidant biomarkers and mineral profile in summer-stressed …
YA Amin, EMA El-Naga, EA Noseer, SS Fouad, RA Ali
Theriogenology 134, 34-41 , 2019
2019.0
Citations: 19
Investigating polymorphisms and expression profile of immune, antioxidant, and erythritol-related genes for limiting postparturient endometritis in Holstein cattle
M Al-Sharif, M Abdo, OE Shabrawy, EMA El-Naga, L Fericean, ...
Veterinary Sciences 10 (6), 370 , 2023
2023.0
Citations: 13
Soybean lecithin–based extender improves Damascus goat sperm cryopreservation and fertilizing potential following artificial insemination
M Fathi, R Zaher, D Ragab, I Gamal, A Mohamed, E Abu-El Naga, M Badr
Asian Pacific Journal of Reproduction 8 (4), 174-180 , 2019
2019.0
Citations: 13
Diagnosis of Brucellosis in Recently Aborted Ewes Using Serological Tests and Polymerase Chain Reaction
Hassan Ali Hussein, Ragab Hassan Mohamed, Aml Mohammed Abdel-Ra`ouf, Eman ...
Journal of Applied Sciences 19 (2), 77-81 , 2019
2019.0
Citations: 13
Effect of different post-partum therapeutic protocols with intrauterine oxytetracycline, oxytocin and/or GnRH injection in post-kidding goats on oxytetracyclines residues in …
AM Zakaria, T Al-Daek, E Elmeligy, RH Mohamed, EMA El-Naga, ...
BMC veterinary research 19 (1), 139 , 2023
2023.0
Citations: 7
Changes in the fetal fluids compositions during dystocia of dairy buffaloes
YA Amin, EA Noseer, EMA El-Naga
Adv. Anim. Vet. Sci 8 (7), 728-734 , 2020
2020.0
Citations: 6
DNA polymorphisms and MRNA levels of immune biomarkers as candidates for inflammatory postpartum disorders susceptibility in Italian buffaloes
A Ateya, FA Safhi, H El-Emam, MQ Al-Ghadi, M Abdo, L Fericean, R Olga, ...
Veterinary Sciences 10 (9), 573 , 2023
2023.0
Citations: 4
Effect of histidine and L-Tyrosine supplementation in maturation medium on in-vitro developmental outcomes of buffalo oocytes
EMA El-Naga, ME Ali, RA Sindi, HA Hussein
BMC Veterinary Research 20 (1), 414 , 2024
2024.0
Citations: 3
Cleavage and in vitro cultivation rates monitoring in culture media supplemented with energy sources, non-essential amino acids, and antioxidants in the buffalo embryos
EM Abu El-Naga, ME Ali, RH Ali, HF Hozyen, HA Hussein
BMC Veterinary Research 20 (1), 521 , 2024
2024.0
Citations: 2
Foetal Mummification in Pregnant Dairy Cows Induces Variant Changes on the Hormonal Profile, Biochemical Parameters and Mineral Profile of the Dam
YA Amin, O Shanab, IS Zahran, F Farrag, MA Fawy, M Shukry, ...
Veterinary Medicine and Science 11 (3), e70304 , 2025
2025.0
Citations: 1
L-Carnitine-Mediated Antioxidant Defence in Buffalo Oocytes: A Novel Approach for Improving In Vitro Maturation and Embryo Developmental Competence
EM Abu El-Naga, DA Soliman, MK Hussein, RH Mohamed, A Monir, ...
Egyptian Journal of Veterinary Sciences, 1-9 , 2025
2025.0
Effect of histidine and L-Tyrosine supplementation in maturation medium on in-vitro developmental outcomes of buffalo oocytes
EMAEN Eman M. Abu El-Naga, Montaser Elsayed Ali, Ramya A. Sindi, Hassan A ...
BMC veterinary , 2024
2024.0
addition of histidine and L-tyrosine to maturation and culture media on IVM, IVF and IVC of buffalo oocytes and embryos
EMA El-Naga, ME Ali, RA Sindi, HA Hussein
2023.0
Effect of Enrichment of
SM Zaabel, EM Abu-El Naga, H El-Sheikh Ali, AZ Balboula, ...
Vitro , 2017
2017.0
Mummification of the fetus in pregnant cows induces variant biological changes in the body
Y Amin, O Shanab, IS Zahran, F Farrag, MA Fawy, BD Ioan, L Fericean, ...
Frontiers in Veterinary Science 11, 1463388 , 0
Effect of sperm motility enhancers on in vitro fertilization and embryo development of buffalo oocytes
EM Abu-El Naga, HES Ali, MR Badr, AM El, SMZ Desouky