Neurophytomedicine: A Critical Review of Traditional Herbal Anxiolytics Sreya Kosanam, Revathi Boyina, A. M. T. Gurubasavaraj, K. M. D. Muzammil, R. Praveen Javali, Adinarayana Andy, Aman Suresh Tharayil Pharmacology Characterizations Toxicity and Herb Drug Interactions of Herbs in Traditional Medicine, 2026 This chapter examines the scientific and clinical foundations of traditional herbal anxiolytics within the emerging field of neurophytomedicine. It explores the global prevalence and neurobiology of anxiety disorders, limitations of conventional pharmacological treatments, and the therapeutic potential of botanicals with historical use in anxiety relief. Emphasis is placed on phytochemical classes such as flavonoids, terpenes, alkaloids, and saponins, and their mechanisms of action on GABAergic, serotonergic, and hypothalamic pituitary adrenal systems. Comparative clinical evidence, safety considerations, herb-drug interactions, and regulatory frameworks are critically reviewed. The chapter also addresses challenges in standardization, placebo effects in trials, and ethical sourcing of medicinal plants. Finally, it outlines future directions including pharmacogenomic personalization, AI-driven phytochemical discovery, and integration of validated botanicals into mainstream psychiatry.
Decoding EMT through liquid biopsy: A path to early detection and targeted therapy Revathi Boyina, Prasanna Kumar Desu, Sreya Kosanam, Anusha Rapuri, Ramesh Alluri, Jadala Shankaraswamy, Sri Chandana Mavulati, Vanitha Kondi Advances in Cancer Biology Metastasis, 2025 : Epithelial-mesenchymal transition (EMT) is a key biological process that enables cancer cells to acquire invasive, migratory, and therapy-resistant properties, driving metastasis and poor clinical outcomes. Traditional tissue biopsies, while informative, provide only static and localized snapshots of tumors, limiting their ability to capture dynamic changes like EMT. Liquid biopsy has emerged as a powerful, minimally invasive tool to monitor tumor evolution in real time by analyzing circulating tumor cells (CTCs), cell-free DNA (cfDNA), circulating tumor DNA (ctDNA), and extracellular vesicles (EVs) from body fluids. This review highlights the molecular mechanisms that govern EMT, including transcriptional, signaling, and epigenetic regulation, and discusses how EMT-associated alterations can be detected through liquid biopsy. We explore the clinical applications of EMT monitoring via liquid biopsy for early detection of metastasis, prognostic assessment, therapy selection, and monitoring minimal residual disease (MRD). Despite current challenges such as the biological complexity of EMT, detection sensitivity, and the need for standardization, technological advances and emerging computational tools are paving the way for the integration of liquid biopsy into precision oncology. Understanding and decoding EMT through liquid biopsy represents a promising frontier for improving cancer diagnosis, prognosis, and therapeutic strategies, offering hope for more personalized and effective cancer management in the future.
Marine-Derived Chemotherapeutic Agent for Acute Leukemia: In Silico and In Vitro Evaluation Revathi Boyina, Sreya Kosanam, Kishore Babu M Chemistry and Biodiversity, 2025 Acute leukemia, a severe hematological malignancy marked by abnormal white blood cell proliferation, often resists conventional therapies. The growing interest in natural therapeutics has spotlighted marine‐derived compounds for their unique bioactivities. This study explores the therapeutic potential of Ascophyllum nodosum extract, focusing on Laminine, in modulating key leukemia‐associated proteins, lactate dehydrogenase (LDH), and phosphoinositide 3‐kinase (PIK3), using in silico and in vitro approaches. Molecular docking was performed to assess Laminine's binding interactions with LDH and PIK3. An in vitro leukemia model was adopted using N‐Nitroso‐N‐ethylurea‐treated Wistar rats. Efficacy was evaluated through LDH release, PIK3 activity, hematological parameters, and clinical signs of leukemia. Docking studies revealed moderate binding affinities (−6.5 kcal/mol) of Laminine with LDH and PIK3, suggesting potential therapeutic interactions. In vitro, the extract demonstrated dose‐dependent cytoprotective effects, reducing LDH release and restoring PIK3 activity. Leukemia‐induced hematological abnormalities, including elevated white blood cell counts, anemia, and thrombocytopenia, were partially reversed. The highest dose (50 µM) showed maximal efficacy in reducing cytotoxicity and modulating signaling pathways. A. nodosum extract, particularly Laminine, shows potential as a therapeutic candidate against leukemia by targeting metabolic and signaling pathways. Further research is needed to optimize its clinical utility and elucidate its mechanisms.
Assessment of Hepatoprotective Potential of Sapindus emarginatus Vahl. — A Pharmacognostic and Phytochemical Study T. Santhi Priya, Revathi Boyina, B. Sasidhar, G. Raveendra Babu, Ch. Devadasu, M. Jalaih, M. Kishore Babu Toxicology International, 2025 This study aimed to evaluate the hepatoprotective potential of drugs from herbal origins. Using a stepwise pharmacognostic and phytochemical approach, plant material was standardized to improve the purity, safety, and efficacy of herbal medicine for treating Drug-Induced Hepatotoxicity (DIHT). The literature review identified plants from folklore medicine that have hepatoprotective and nephroprotective effects but are not documented. So, the present research focused on the hepatoprotective activity of the drug by evaluating the alanine aminotransferase, alanine phosphatase, total protein, cholesterols and bilirubin levels. First the bark of the plant S. emarginatus Vahl., was studied for pharmacognostic evaluation and phytochemical evaluation by using various tests. Animals were divided into 5 groups with six animals in each group namely, control, standard control, toxicity control, ethanolic extract and water extract for pharmacological evaluation. Treatment with hepatotoxic (paracetamol) led to the depletion of glutathione and reduction in non-protein sulfhydryl moiety, which is further increased, were observed by treating with ethanolic and water extracts compared to the positive standard group. The key findings of this study highlight the potential of traditional folk medicine, in treating drug-induced hepatotoxicity. The findings of this study highlight the need for further research in identifying natural alternative medicines to treat drug-induced hepatotoxicity. Major Findings: The novelty of this research lies in its comprehensive exploration of the hepatoprotective potential of Sapindus emarginatus Vahl. bark, which has not been documented despite its traditional use. The study employs a systematic standardization approach to ensure the quality and efficacy of the plant material, bridging traditional knowledge with modern scientific validation. Additionally, the in vivo evaluation against paracetamol-induced liver toxicity provides novel experimental evidence, offering insights into the plant's mechanisms of action and supporting its potential as a source of hepatoprotective phytochemicals.
In-vitro and In Silico Assessment of Anti-inflammation Properties of Saponarin Extracted from Hordeum Vulgare Revathi Boyina, Sreya Kosanam, Sasidhar Bhimana, Raveendra Babu Gudimitla, Dhachinamoorthi Duraiswamy Anti Inflammatory and Anti Allergy Agents in Medicinal Chemistry, 2024 Background: Hordeum vulgare, commonly known as Barley grass, is a historically significant cultivated plant with profound implications for societies, agricultural sciences, and human nutrition. It has been valued for both sustenance and its potential medicinal properties Objective: This study aims to comprehensively investigate the medicinal properties of Hordeum vulgare, focusing on its potential therapeutic benefits and anti-inflammatory properties. Addition-ally, we seek to quantify and compare the phytochemical content of two distinct extracts: Barley Grass Hexane Extract (BGHE) and Barley grass aqueous extract (BGAQ). Methods: We quantified the phytochemical contents of BGHE and BGAQ and evaluated their anti-inflammatory effects using UV spectroscopy at 560 nm, coupled with the RBC membrane stabilization technique. Subsequently, we conducted in silico studies to assess the in vitro anti-inflammatory potential of Barley grass leaf extracts. Results: Both BGHE and BGAQ demonstrated significant inhibitory effects on inflammation com-pared to the control group. However, BGHE exhibited superior anti-inflammatory efficacy when compared to BGAQ, suggesting its role as a potential anti-inflammatory agent. In silico studies further supported the anti-inflammatory potential of Barley grass leaf extracts. Conclusion: Hordeum vulgare, or Barley grass, offers a wealth of health benefits, including anti-inflammatory, anti-diabetic, anti-cancer, antioxidant, anti-acne, and anti-depressant properties. These properties contribute to improved immunity, reduced cardiovascular disorders, and alleviation of fatigue. The distinct extracts, BGHE and BGAQ, both exhibit promising anti-inflammatory capabilities, but BGHE shows better anti-inflammatory activity. This research sheds light on the therapeutic potential of Barley grass, making it a valuable candidate for further exploration in the field of natural medicine.
Agaricus Subrufescens ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with Letrozole induced polycystic ovarian syndrome Sarad Pawar Naik Bukke, Bharghava Bhushan Rao Pathange, Srikanth Kumar Karumanchi, Jalaiah Marri, Revathi Boyina, Kavya Rachamsetty, Bhargavi Manchikalapati, Saidi Odoma, Bello Hussaini Journal of Ovarian Research, 2023 Objective The objective of this study is to investigate the effects of an ethanolic extract derived from Agaricus subrufescens on rat models exhibiting Polycystic Ovarian Syndrome (PCOS) induced by Letrozole. Methods A total of thirty female Wistar rats were divided into five groups, each consisting of six rats. The negative control group was administered a volume of 1 mL of a 0.5% solution of carboxy methylcellulose (CMC). Letrozole (1 mg/kg) was administered to additional groups for a duration of 21 days in order to induce polycystic ovary syndrome (PCOS). Animals designated as positive controls were euthanized on the 22nd day. Both the test group and the standard group were subjected to treatment from the 22nd day to the 36th day. The experimental group was administered ethanolic extract of Agaricus subrufescens at doses of 200 mg/kg and 400 mg/kg p.o, while the control group received clomiphene citrate at a dose of 1 mg/kg. The study observed various physiological markers in individuals with polycystic ovarian disease, including estimated blood glucose levels, total cholesterol levels, triglyceride levels, and hormonal fluctuations such as increased testosterone and estrogen levels, as well as decreased progesterone levels. The presence of menstrual irregularities was confirmed through the examination of vaginal smears and histopathological changes in the ovaries. Results The consumption of Agaricus subrufescens was found to have a significant impact on various physiological parameters, including blood glucose levels, testosterone levels, anovulation, and menstrual irregularity. All therapeutic interventions significantly normalized the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT). The rats with polycystic ovary syndrome (PCOS) that were induced by Letrozole exhibited increased levels of urea and creatinine. The findings of this study indicate that the administration of Agaricus subrufescens therapy has a protective effect on renal function, as evidenced by a reduction in serum levels of urea and creatinine. In rats with polycystic ovary syndrome (PCOS) induced by Letrozole, the inhibition of hepatic synthesis, promotion of ovarian follicle immaturity, and elevation of androgen secretions result in an increase in the weight of the liver and ovaries. The weight of endocrine organs exhibited a decrease across all treatment groups. The histopathological examination of PCOS specimens revealed an increased presence of cysts and theca lutein cells. The group of rats with polycystic ovary syndrome (PCOS) that did not receive treatment exhibited a higher number of cysts compared to the groups that received treatment. Conclusion This study demonstrated that the administration of Letrozole orally resulted in the development of polycystic ovarian disease. The results indicated heightened levels of blood glucose, total cholesterol, and triglycerides, as well as alterations in hormone levels such as increased testosterone and estrogen, and decreased progesterone. These hormonal changes were accompanied by menstrual irregularities, which were confirmed through the examination of vaginal smears and histopathological analysis of the ovaries in the control group with polycystic ovarian disease. The treatment groups that received Agaricus subrufescens exhibited a decrease in blood glucose, total cholesterol, and testosterone levels.
Insights of In-silico Neurotoxicity Studies of Glucuronolactone, Taurine and Gluconolactone Correlating the Induced Neuronal Alteration in Rat Pups Revathi Boyina, Sujatha Dodoala, Sumalatha Gindi, Pooja Reddy M, Prasanna K Desu International Journal of Drug Delivery Technology, 2023 Background: Signifi cant concentrations of food additives found in energy drinks have the potential to be neurotoxic and promote oxidative stress, among other negative consequences. Pregnant rats were split up into six groups for the current study. Group 1 received vehicle, CAF standard (25 mg/kg p.o.), groups 3-6 received GLUR (5 mg/kg p.o.), TAU (8 mg/kg p.o.), GLU (84 mg/kg p.o.), and combinations of the three chosen food additives (CF), respectively. From prenatal day 3 through postnatal day 15, certain food additives were administered to pregnant rats at signifi cant doses. After parturition on PND 21, behavioral changes were assessed using the Rotarod, active avoidance, and elevated plus maze tests. On PND 30, 45, and 60, rat brain tissue had its acetylcholine and epinephrine levels evaluated. Further, on days 30 and 60, brain tissue was assessed for the presence of oxidative stress markers such as lipid peroxidation, catalase, superoxide dismutase, and glutathione peroxidase. Finally, histopathological studies were carried out in brain hippocampal region. Further in-silico studies were carried out on selected receptors. Results: Rat pups fed with food additives showed a signifi cant (p &lt 0.001) change in behavior, including memory, cognition, and motor activity. Increased lipid peroxidation and decreased anti-oxidant enzymes were signifi cant in TAU and CF groups. Further in-silico studies were carried out, where GLUR showed high binding affi nity to specifi c receptor targets, GABA A and NMDA1 receptors, and specifi c enzyme targets MAO A and MAO B neurotransmitter metabolic enzymes compared to caff eine hinted the decrease in neurotransmitters as in-vivo studies. Conclusion: The current fi ndings support the hypothesis that the chosen dose and mix of food additives altered rat pups’ neurobehavioral and neurotransmitter profi les.
Intervention of Hypertension and Diabetes in India in Children and Adults RVL Revathi Boyina1*, Ch. Sushma Swaraj2, I. Thanuja3, E. Aashrita4, A.V ... International Journal of Pharmacy and Pharmaceutical Research (IJPPR) 32 … , 2026 2026
Neurotransmitter dysregulation in depression, anxiety, and suicidality: from synaptic dysfunction to cellular pathogenesis KSVAB Ganesh, AK Verma, SS Sinha, R Boyina, S Kosanam Psychiatry Research, 117120 , 2026 2026 Citations: 1
The Suprachiasmatic Nucleus-Habenula Axis: A Circadian Mechanism for Suicide Risk and Mood Dysregulation KSVAB Ganesh, AK Verma, SA Shreha, SS Sinha, R Boyina, S Kosanam Behavioural Brain Research, 116025 , 2026 2026
Neurophytomedicine: A Critical Review of Traditional Herbal Anxiolytics S Kosanam, R Boyina, AMT Gurubasavaraj, KMD Muzammil, RP Javali, ... Pharmacology, Characterizations, Toxicity, and Herb-Drug Interactions of … , 2026 2026
Decoding EMT through liquid biopsy: A path to early detection and targeted therapy R Boyina, PK Desu, S Kosanam, A Rapuri, R Alluri, J Shankaraswamy, ... Advances in Cancer Biology-Metastasis 15, 100141 , 2025 2025 Citations: 2
BioID: Novel ML-Driven Framework for Non-Invasive Blood Group Detection S Muvvala, C Himabindu, B Sasidhar, B Revathi, M Kishorebabu, ... 2025 IEEE International Conference on Technology, Informatics, Management … , 2025 2025
Assessment of Hepatoprotective Potential of Sapindus emarginatus Vahl. — A Pharmacognostic and Phytochemical Study MJMKB T. Santhi Priya, Revathi Boyina, B. Sasidhar, G. Raveendra Babu, Ch ... Toxicology International 32 ((1)), 139-146 , 2025 2025
Marine‐Derived Chemotherapeutic Agent for Acute Leukemia: In Silico and In Vitro Evaluation R Boyina, S Kosanam Chemistry & Biodiversity, e202403329 , 2025 2025
Text book of Community Pharmacy R Nagaharikrishna 2024
Comparative Effectiveness of Nifedipine and Labetalol for The Management of Pregnancy-Induced Hypertension: An Observational Study BM Kavya Rachamsetty 1* Revathi Boyina 2, Sreya Kosanam3 Advances in Bioresearch 15 (6), 374-378 No.44 , 2024 2024
A TEXT BOOK ON PHARMACOLOGY Dr.B. Revathi ,Dr.R. Kavya,i, Dr. M. Bhargavi 2024
A Review on Diagnosis and Management of Myasthenia Gravis TD Revathi Boyina,1 Venkata Dharani Cheemaladinne Journal of Hospital Pharmacy 1 (January Supplement Issue-2)), Page-1 , 2024 2024
A Review on Diagnosis and Management of Myasthenia Gravis TD Revathi Boyina, Venkata Dharani Cheemaladinne Hospital Pharmacy 19 (1), 1 , 2024 2024
In-vitro and In Silico Assessment of Anti-inflammation Properties of Saponarin Extracted from Hordeum Vulgare R Boyina, S Kosanam, S Bhimana, RB Gudimitla, D Duraiswamy Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistryrrent … , 2024 2024 Citations: 4
THERAPEUTIC POTENTIALS OF MARINE PLANT ON VARIOUS DISEASES SRM Revathi Boyina*, Sai Lakshmi M , Jahnavi G , Likhita G , Farahana P JASC: Journal of Applied Science and Computations 11 (Issue II), 1-14 , 2024 2024
Neuropharmacological Profiling of Acanthus ilicifolius: A Coastal Mangrove's Potential in CNS Depression and Analgesia B Revathi, N Danamurthy, T Sujatha, M Jalaiah Indo-American Journal of Life Sciences and Biotechnology 21 (1), 34-40 , 2024 2024
COMPARATIVE EFFECTIVENESS OF NIFEDIPINE AND LABETALOL FOR THE MANAGEMENT OF PREGNANCY-INDUCED HYPERTENSION: AN OBSERVATIONAL STUDY BM Kavya Rachamsetty*, Revathi Boyina, Sreya Kosanam ADVANCES IN BIORESEARCH 15 (6) , 2024 2024
COMPARATIVE EFFECTIVENESS OF NIFEDIPINE AND LABETALOL FOR THE MANAGEMENT OF PREGNANCY-INDUCED HYPERTENSION: AN OBSERVATIONAL STUDY BM Kavya Rachamsetty*, Revathi Boyina, Sreya Kosanam ADVANCES IN BIORESEARCH 15 (6) , 2024 2024
COMPARATIVE EFFECTIVENESS OF NIFEDIPINE AND LABETALOL FOR THE MANAGEMENT OF PREGNANCY-INDUCED HYPERTENSION: AN OBSERVATIONAL STUDY BM Kavya Rachamsetty*, Revathi Boyina, Sreya Kosanam ADVANCES IN BIORESEARCH 15 (6) , 2024 2024
MOST CITED SCHOLAR PUBLICATIONS
Antiurolithiatic and invitro anti-oxidant activity of leaves of Ageratum conyzoides in rat S Gindi, T Methra, BR Chandu, R Boyina, V Dasari World J. Pharm. Pharm. Sci 2, 636-49 , 2013 2013 Citations: 52
Evaluation of the Neurobehavioural Toxic Effects of Taurine, Glucuronolactone, and Gluconolactone Used in Energy Drinks in Young Rats R Boyina, S Dodoala Turkish Journal of Pharmaceutical Sciences 17 (6), 659-666 , 2020 2020 Citations: 18
Drug-induced liver injury: A review S Kosanam, R Boyina International Journal of Pharmacological Research 5 (2), 24-30 , 2015 2015 Citations: 18
Interpenetrating polymer network (IPN) microparticles an advancement in novel drug delivery system: a review MT Margaret, B Brahmaiah, PV Krishna, B Revathi, S Nama Int J Pharm Res Bio Sci 2 (3), 215-24 , 2013 2013 Citations: 9
ASSESSMENT OF IN VITRO ANTI INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OF Ipomoea batatas TUBERS ANB T. Mercy Margaret*, P. Krishna, B. Revathi, D. Eswar Tony, M. Sathish Kumar Asian Journal of Research in Biological and Pharmaceutical Sciences 1 (1), 47-53 , 2013 2013 Citations: 9
Agaricus Subrufescens ameliorates ovarian dysfunction and regulates altered biochemical parameters in rats with Letrozole induced polycystic ovarian syndrome SPN Bukke, BBR Pathange, SK Karumanchi, J Marri, R Boyina, ... Journal of Ovarian Research 16 (1), 221 , 2023 2023 Citations: 8
Evaluation of anti–diarrheal activity of aqueous extract of Trigonella foenum–graecum R Boyina, S Kosanam, TT Rani Int J Pharmacol Res 4, 130-3 , 2014 2014 Citations: 7
In-vitro and In Silico Assessment of Anti-inflammation Properties of Saponarin Extracted from Hordeum Vulgare R Boyina, S Kosanam, S Bhimana, RB Gudimitla, D Duraiswamy Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistryrrent … , 2024 2024 Citations: 4
Evaluation of Antibacterial activity of aqueous extract of Cucumis melo. DD Revathi B, Sasidhar B, Babu GR, Divyalatha J, Devadasu C Research Journal of Pharmacognosy and Phytochemistry 15 (1), 1-4 , 2023 2023 Citations: 4
Decoding EMT through liquid biopsy: A path to early detection and targeted therapy R Boyina, PK Desu, S Kosanam, A Rapuri, R Alluri, J Shankaraswamy, ... Advances in Cancer Biology-Metastasis 15, 100141 , 2025 2025 Citations: 2
Effects of food additives on the mitochondria integrity and functioning R Boyina, S Dodoala, S Gindi Mitochondrial Intoxication, 475-491 , 2023 2023 Citations: 2
Revealing facts about CAR-T cell therapy DS Padmanjali, B Revathi, M Jalaiah ImmunoAnalysis 2 (1), 6-6 , 2022 2022 Citations: 2
Neurotransmitter dysregulation in depression, anxiety, and suicidality: from synaptic dysfunction to cellular pathogenesis KSVAB Ganesh, AK Verma, SS Sinha, R Boyina, S Kosanam Psychiatry Research, 117120 , 2026 2026 Citations: 1
A Rare Hepatic Vascular Disorder Budd Chiari Syndrome K Vindhya, MS Amrutha, R Boyina, D Dacchinamoorthi, ... International Journal of All Research Education and Scientific Methods … , 2024 2024 Citations: 1
Neurodevelopmental Toxic Effects of Food Additives Used in Energy Drinks on Developing Rats R Boyina, S Dodoala Pharmaceutical Sciences 27 (2), 183-193 , 2021 2021 Citations: 1
Antiurolithiatic and in vitro anti-oxidant activity of leaves of Ageratum conyzoides in rat. SG Sumalatha Gindi, TM Tanuja Methra, BR Chandu, RB Revathi Boyina, ... 2013 Citations: 1
TARGETING OF ANTI CANCER DRUGS THROUGH NANOPARTICLES CB Thumati Thirumala Rani*, B. Brahmaiah, B. Revathi, Sreekanth Nama International Journal of Pharmacy & Therapeutics 4 (3), 154-159 , 2013 2013 Citations: 1
Intervention of Hypertension and Diabetes in India in Children and Adults RVL Revathi Boyina1*, Ch. Sushma Swaraj2, I. Thanuja3, E. Aashrita4, A.V ... International Journal of Pharmacy and Pharmaceutical Research (IJPPR) 32 … , 2026 2026
The Suprachiasmatic Nucleus-Habenula Axis: A Circadian Mechanism for Suicide Risk and Mood Dysregulation KSVAB Ganesh, AK Verma, SA Shreha, SS Sinha, R Boyina, S Kosanam Behavioural Brain Research, 116025 , 2026 2026
