Dr. Amit Ravindra Nisal

@mcpune.bharatividyapeeth.edu

Associate Professor in Pathology
Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune

Dr. Amit Ravindra Nisal


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https://researchid.co/dramitnisal
Trainings and Work Experience:
1. Worked as Tutor in Pathology at KEM Hospital, Pune and trained for bone marrow and special hematology techniques under the guidance of Dr. Rumma Manchanda; eminent haematopathologist from Pune in KEM Hospital as well as in her private laboratory for about 10 years.
2. Worked as Junior Pathologist at Deenanath Mangeshkar Hospital, Pune for 2.5 years.
3. I am currently working as Associate Professor in Department of Pathology, Bharati Vidyapeeth (DTU) Medical College having 880 bedded super-specialty Hospital with undergraduate and postgraduate teaching programme.
4. I am involved in routine as well as special haematology reporting in the laboratory with special interest in bone marrow procedure and reporting, flow cytometry and HPLC.
5. NABL ISO 15189:2012 Four days of internal auditor course at Bharati Hospital from 22-25th June 2016.
6. I am currently in charge of Haematology Labs and course coordinator for Diagnostic Haematology fellowship.

EDUCATION

M.D. (Pathology)- Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune (2015)
D.C.P. - Rural Medical College, Loni, University of Pune (2002)
Fellowship Diagnostic Haematology - Bharati Vidyapeeth (Deemed To Be) University Medical College Hospital and Research Centre, Pune (2019)

RESEARCH INTERESTS

To work in the area of Hematopathology with advance techniques and provide quality results to the patients and treating clinicians. Areas of interest are Neonatal hematology, Bone marrow, Flow cytometry, Coagulation and HPLC.
7

Scopus Publications

37

Scholar Citations

3

Scholar h-index

1

Scholar i10-index

Scopus Publications

  • Emerging Role of Monocyte Distribution Width for Early Sepsis Detection in Adult Intensive Care
    Ayushi Raghuvanshi, Amit Nisal, Reena Bharadwaj
    Indian Journal of Hematology and Blood Transfusion, 2025
  • Analysis of hematology quality control using six sigma metrics
    Shreya Goel, Amit R. Nisal, Ankita Raj, Ravindra C. Nimbargi
    Indian Journal of Pathology and Microbiology, 2024
    Introduction: Clinical laboratories serve a critical role in increasing the efficiency of patient care. Choosing the right test, getting trustworthy results and appropriate interpretation are of utmost importance in improving the patient’s well-being. Quality management strategies should be applied in routine patient care because laboratory errors have a major impact on the quality of patient care. In sigma metrics, errors identified are quantified as percentage errors or defects per million (DPM). It aims at improving the quality control (QC) process by forming an appropriate strategy. Aim and Objectives: To analyze the internal quality control (IQC) of hematology analytes using the sigma metrics method and to devise the frequency of IQC by the results of six sigma metric analysis. Materials and Methods: This study was conducted in a tertiary care center of western India. Internal quality control (IQC) data sets of five analytes- Red Blood Cell count (RBC), Hemoglobin (Hb), Hematocrit (Hct), White blood cell count (WBC), and Platelet count (PLT) were analyzed retrospectively of six months using Beckman Coulter DXH 800 hematology analyzers. Results: The observed sigma value was >6 for Hb, TLC, and PLT, indicating excellent results and requiring no modification in IQC. The Sigma value was between 3 and 4 for RBC and Hct suggested the need for improvement in quality control (QC) processes. No analytes showed a Sigma value of <3. Conclusion: Sigma metrics provide a quantitative framework that helps to assess analytic methodologies and can serve as an important self-assessment tool for quality assurance in the clinical laboratory.
  • Neutrophil CD64 - A Reliable Predictive Marker of Sepsis in Adult Intensive Care Unit
    Rohan Waykole, Amit Ravindra Nisal, Anu Christopher, Nishtha Danu, Ravindra C Nimbargi
    Journal of Applied Hematology, 2024
    BACKGROUND: Sepsis poses a significant threat to adult patients admitted to intensive care units (ICUs), contributing to substantial morbidity and mortality. Neutrophil CD64 (nCD64) expression has been linked to inflammatory responses during infection or tissue injury, suggesting its potential as a predictive marker for sepsis. MATERIALS AND METHODS: We conducted a prospective analytical study over 2 years at a tertiary care hospital, enrolling 91 sepsis cases from the adult ICU. Alongside routine laboratory parameters, including complete blood count, prothrombin time, activated partial thromboplastin time, fibrinogen, C-reactive protein, and procalcitonin, the expression of nCD64 was analyzed using a Beckman Coulter Navios flow cytometer. Median fluorescence intensity (MFI) was calculated for these cases. Statistical analysis was performed using SPSS version 25.0 software, with a significance threshold of P < 0.05. RESULTS: MFI scores demonstrated a notable increase in diagnosed sepsis cases, as determined by clinical and biochemical parameters. Moreover, changes in MFI scores on Day 4 exhibited a correlation with other clinical and biochemical parameters, strengthening the association between nCD64 expression and sepsis severity. CONCLUSION: Our findings suggest that nCD64 serves as an independent prognostic factor in adult ICU sepsis patients. It offers a promising alternative to traditional sepsis markers for predicting patient outcomes, emphasizing its potential clinical utility in guiding therapeutic interventions and improving patient care in ICU settings.
  • Analysis of APTT Based Clot Waveform Parameters in Various Clinical Conditions – A Study at A Tertiary Care Center
    Rachana Lakhe, Amit Nisal, Preeti Doshi, Ravindra Nimbargi
    Indonesian Journal of Medical Laboratory Science and Technology, 2023
    Various coagulation tests like Prothrombin Time (PT) and Activated Partial Thromboplastin Time (APTT) are estimated by automated coagulation analyzers. The newer fully automated analyzers generate clot wave forms aPTT-CWA for these parameters are derived. In this study, the objective was to analyze clot wave form characteristics morphology and its first and second derivative values in cases with abnormal APTT. ACL TOP 300 generated curves for APTT in a total 125 patients with 20 normal controls are included. First derivative, second derivative, morphology of curve: sigmoid, biphasic, prolonged pre-coagulation phase, second derivative morphology like early and late shoulder, biphasic peak, delayed deceleration were the analyzed parameters. Wave clot forms of 125 patients were included in this study. Patients (M:F - 2.2:1, mean age: 46.9 ± 20 years). A spectrum of clinical conditions was Covid (20%), liver disease (23%), polytrauma (10.4%), cardiac diseases (8.8%), sepsis/DIC (7.2%), thromboembolism (7.2%), renal diseases (6.4%), bacterial infections (4%), dengue (4%), snake bite (1.6%) and factor deficiency (1.6%). Liver and heart disease showed a significant difference in acceleration and deceleration peaks followed by sepsis, dengue, polytrauma and sepsis/DIC. Deceleration peak was prolonged in patients of Covid (p<0.05). Sepsis and liver diseases showed prolonged first derivative peak (p<0.05). CWA is very easily available on all automated coagulation analyzers. It is inexpensive with fast turn round time. Both quantitative as well as qualitative informations such as velocity, acceleration of clot formation and wave pattern details were recorded. Our study highlights importance of quantitative and qualitative CWA parameters acquired by performing APTT test for the automated analyzers.
  • Secondary Hemophagocytic Lymphohistiocytosis in an Infant with Wolman Disease
    Sandip Bartakke, Amit Nisal, Vibha Bafna, Aashna Valecha
    Indian Journal of Hematology and Blood Transfusion, 2021
  • Triple trouble - Macrophage activation syndrome in a case of severe leptospirosis and scrub typhus co-infection
    Journal of Association of Physicians of India, 2014
  • Gaucher's Disease in Adults
    Pravara Medical Review, 2011

RECENT SCHOLAR PUBLICATIONS

  • Haematological Parameters, Coagulation Profile and Serum Lactate Dehydrogenase in Women with Pregnancy Induced Hypertension versus Normotensive Pregnant Women: A Case-control …
    SP SARATHI SARKAR, AR NISAL, PD SHELKE, PR DOSHI, ...
    Journal of Clinical & Diagnostic Research 19 (10) , 2025
    2025
  • Emerging Role of Monocyte Distribution Width for Early Sepsis Detection in Adult Intensive Care
    A Raghuvanshi, A Nisal, R Bharadwaj
    Indian Journal of Hematology and Blood Transfusion, 1-5 , 2025
    2025
  • Diagnostic Value of Platelet Distribution Width in Acute Coronary Syndrome: A Comparison with Troponin I.
    P Yadav, A Patil, E Bhushan, RC Nimbargi, A Nisal, R Bharadwaj
    Journal of Contemporary Clinical Practice 11, 106-111 , 2025
    2025
  • Chromatographic insights into hemoglobinopathies: Spectrum analysis by high-performance liquid chromatography in a western Indian tertiary care hospital
    P Shelke, P Doshi, A Nisal, A Momin, R Nimbargi
    Medical Laboratory Journal 19 (2), 1-5 , 2025
    2025
  • Unveiling Dengue Lab profiles: A comprehensive Institutional analysis.
    Ankita Raj, Amit Nisal, Shreya Goel, Subrata Rath, Meera Modak
    International Journal of Research and Analytical Reviews 12 (1), 868-75 , 2025
    2025
  • Spectrum of Mutations in Beta-Thalassemia in Maharashtrian Population. Journal of Chemical Health Risks
    Shyla, Nisal Amit, Christopher Anu, Patil Anuja, Kesarkhane Milind, Reena ...
    Journal of Chemical Health Risks. 14 (6), 369-75 , 2024
    2024
  • Distribution of thrombocytopenia etiologies in adults:-A study of 500 cases in a tertiary care centre.
    Khushboo Jethvani, Rachana Lakhe, Amrutraj Patil, Amit Nisal, Reena Bharadwaj
    International Journal of Scientific Research. 13 (9), 52-53 , 2024
    2024
  • Neutrophil CD64–A Reliable Predictive Marker of Sepsis in Adult Intensive Care Unit
    R Waykole, AR Nisal, A Christopher, N Danu, RC Nimbargi
    Journal of Applied Hematology 15 (3), 204-208 , 2024
    2024
  • Analysis of hematology quality control using six sigma metrics
    S Goel, AR Nisal, A Raj, RC Nimbargi
    Indian Journal of Pathology and Microbiology 67 (2), 332-335 , 2024
    2024
    Citations: 7
  • Cell Block versus Cytospin in the Detection of Malignancy in Body Fluids: A Cross-sectional Study.
    D MASHRU, A NISAL, R LAKHE, S MULAY, R NIMBARGI
    Journal of Clinical & Diagnostic Research 17 (7) , 2023
    2023
    Citations: 2
  • Haematological parameters in preterm neonates admitted in neonatal intensive care unit in a tertiary care hospital
    DK Parikh, AR Nisal, RC Nimbargi
    Medical Laboratory Technology Journal 9 (1) , 2023
    2023
    Citations: 1
  • Analysis of APTT based clot waveform parameters in various clinical conditions–a study at a tertiary care center
    R Lakhe, A Nisal, P Doshi, R Nimbargi
    Indonesian Journal of Medical Laboratory Science and Technology 5 (1), 1-9 , 2023
    2023
    Citations: 2
  • Bone marrow cellularity in pancytopenia- A paradigm of underlying pathology.
    RN Alok Kumar, Parineeta Shelke, Preeti Doshi, Amit Nisal
    National Journal of Laboratory Medicine 12 (2), 45-49 , 2023
    2023
  • Linezolid-Induced Ringed Sideroblastic Anemia and Thrombocytopenia in a Child with Extensively Drug-Resistant Tuberculosis
    S Sippy, A Nisal, BU Sarangi
    Indian Pediatrics Case Reports 1 (4), 257-259 , 2021
    2021
  • Coagulopathy associated with Hepato-renal disorders and its significance in critically ill patients
    V Kale, A Nisal, A Kelkar, R Nimbargi
    IP Journal of Diagnostic Pathology and Oncology 6 (2), IP Innovative … , 2021
    2021
  • Histomorphological mimickers of benign prostatic lesions with prostatic adenocarcinoma
    PS Murgod, PR Doshi, AR Nisal, RC Nimbargi
    Journal of Pathology of Nepal 11 (1), 1859-1863 , 2021
    2021
    Citations: 1
  • Secondary hemophagocytic lymphohistiocytosis in an infant with wolman disease
    S Bartakke, A Nisal, V Bafna, A Valecha
    Indian Journal of Hematology and Blood Transfusion 37 (1), 191-192 , 2021
    2021
    Citations: 5
  • Analysis of patients of hemophagocytic lymphohistiocytosis secondary to infections
    AG Diwan, SS Barsode, AR Nisal, RS Deshpande, MD Patil, NS Shettar, ...
    International Journal of Advances in Medicine 7 (10), 1515 , 2020
    2020
    Citations: 1
  • Absolute lymphocyte count as a cost effective alternative to CD4 count in HIV positive patients
    A Nisal, A Dhabe, A Kelkar, A Christopher, R Nimbargi
    2020
    Citations: 1
  • AntenatalScreeningfor Haemoglobinopathies by Using High Performance Liquid Chromatography
    AJ Kelkar, AM Patilº, AR Nisal, A Christopher, RC Nimbargi
    Indian Journal of Pathology: Research and Practice 8 (5) , 2019
    2019

MOST CITED SCHOLAR PUBLICATIONS

  • Triple trouble: macrophage activation syndrome in a case of severe leptospirosis and scrub typhus co-infection
    AG Diwan, R Shewale, S Iyer, A Nisal, P Agrawal
    JAPI 62, 58-61 , 2014
    2014
    Citations: 13
  • Analysis of hematology quality control using six sigma metrics
    S Goel, AR Nisal, A Raj, RC Nimbargi
    Indian Journal of Pathology and Microbiology 67 (2), 332-335 , 2024
    2024
    Citations: 7
  • Secondary hemophagocytic lymphohistiocytosis in an infant with wolman disease
    S Bartakke, A Nisal, V Bafna, A Valecha
    Indian Journal of Hematology and Blood Transfusion 37 (1), 191-192 , 2021
    2021
    Citations: 5
  • Utility of volume, conductivity and scatter parameters for early diagnosis of neonatal sepsis
    A Kelkar, P Doshi, T Tyagi, A Nisal, NS Mani
    Journal of Applied Hematology 8 (3), 105-109 , 2017
    2017
    Citations: 3
  • Cell Block versus Cytospin in the Detection of Malignancy in Body Fluids: A Cross-sectional Study.
    D MASHRU, A NISAL, R LAKHE, S MULAY, R NIMBARGI
    Journal of Clinical & Diagnostic Research 17 (7) , 2023
    2023
    Citations: 2
  • Analysis of APTT based clot waveform parameters in various clinical conditions–a study at a tertiary care center
    R Lakhe, A Nisal, P Doshi, R Nimbargi
    Indonesian Journal of Medical Laboratory Science and Technology 5 (1), 1-9 , 2023
    2023
    Citations: 2
  • Haematological parameters in preterm neonates admitted in neonatal intensive care unit in a tertiary care hospital
    DK Parikh, AR Nisal, RC Nimbargi
    Medical Laboratory Technology Journal 9 (1) , 2023
    2023
    Citations: 1
  • Histomorphological mimickers of benign prostatic lesions with prostatic adenocarcinoma
    PS Murgod, PR Doshi, AR Nisal, RC Nimbargi
    Journal of Pathology of Nepal 11 (1), 1859-1863 , 2021
    2021
    Citations: 1
  • Analysis of patients of hemophagocytic lymphohistiocytosis secondary to infections
    AG Diwan, SS Barsode, AR Nisal, RS Deshpande, MD Patil, NS Shettar, ...
    International Journal of Advances in Medicine 7 (10), 1515 , 2020
    2020
    Citations: 1
  • Absolute lymphocyte count as a cost effective alternative to CD4 count in HIV positive patients
    A Nisal, A Dhabe, A Kelkar, A Christopher, R Nimbargi
    2020
    Citations: 1
  • International society on thrombosis and haemostasis disseminated intravascular coagulation scoring system: Is it a good prognostic indicator in DIC
    A Nisal, R Nimbargi, P Shelke, A Kelkar
    International Journal of Medical Research & Health Sciences 4 (3), 546-550 , 2015
    2015
    Citations: 1
  • Haematological Parameters, Coagulation Profile and Serum Lactate Dehydrogenase in Women with Pregnancy Induced Hypertension versus Normotensive Pregnant Women: A Case-control …
    SP SARATHI SARKAR, AR NISAL, PD SHELKE, PR DOSHI, ...
    Journal of Clinical & Diagnostic Research 19 (10) , 2025
    2025
  • Emerging Role of Monocyte Distribution Width for Early Sepsis Detection in Adult Intensive Care
    A Raghuvanshi, A Nisal, R Bharadwaj
    Indian Journal of Hematology and Blood Transfusion, 1-5 , 2025
    2025
  • Diagnostic Value of Platelet Distribution Width in Acute Coronary Syndrome: A Comparison with Troponin I.
    P Yadav, A Patil, E Bhushan, RC Nimbargi, A Nisal, R Bharadwaj
    Journal of Contemporary Clinical Practice 11, 106-111 , 2025
    2025
  • Chromatographic insights into hemoglobinopathies: Spectrum analysis by high-performance liquid chromatography in a western Indian tertiary care hospital
    P Shelke, P Doshi, A Nisal, A Momin, R Nimbargi
    Medical Laboratory Journal 19 (2), 1-5 , 2025
    2025
  • Unveiling Dengue Lab profiles: A comprehensive Institutional analysis.
    Ankita Raj, Amit Nisal, Shreya Goel, Subrata Rath, Meera Modak
    International Journal of Research and Analytical Reviews 12 (1), 868-75 , 2025
    2025
  • Spectrum of Mutations in Beta-Thalassemia in Maharashtrian Population. Journal of Chemical Health Risks
    Shyla, Nisal Amit, Christopher Anu, Patil Anuja, Kesarkhane Milind, Reena ...
    Journal of Chemical Health Risks. 14 (6), 369-75 , 2024
    2024
  • Distribution of thrombocytopenia etiologies in adults:-A study of 500 cases in a tertiary care centre.
    Khushboo Jethvani, Rachana Lakhe, Amrutraj Patil, Amit Nisal, Reena Bharadwaj
    International Journal of Scientific Research. 13 (9), 52-53 , 2024
    2024
  • Neutrophil CD64–A Reliable Predictive Marker of Sepsis in Adult Intensive Care Unit
    R Waykole, AR Nisal, A Christopher, N Danu, RC Nimbargi
    Journal of Applied Hematology 15 (3), 204-208 , 2024
    2024
  • Bone marrow cellularity in pancytopenia- A paradigm of underlying pathology.
    RN Alok Kumar, Parineeta Shelke, Preeti Doshi, Amit Nisal
    National Journal of Laboratory Medicine 12 (2), 45-49 , 2023
    2023