CuO nanoparticles/polymer nanocomposite hydrogels Isabela Louise Pereira Lopes, Patrícia Avelar Ribeiro, Emanuel Victor Bastos da Fonseca, Andersen Escobar Schlogl, Bruno Henrique Costa, Érika Lorena Fonseca Costa de Alvarenga, Daniela Leite Fabrino, Igor José Boggione Santos Hydrogels for Wound Healing, 2025
Bioreactors: Applications and Innovations for a Sustainable and Healthy Future—A Critical Review Fernanda Palladino, Paulo Ricardo Franco Marcelino, Andersen Escobar Schlogl, Álvaro Henrique Mello José, Rita de Cássia Lacerda Brambilla Rodrigues, Daniela Leite Fabrino, Igor José Boggione Santos, Carlos Augusto Rosa Applied Sciences Switzerland, 2024 Biotechnological processes are essential for developing economies that aim to stand out in future markets. The use of bioreactors is one of the most important unit operations of biotechnological processes, and real-time monitoring of bioreactors is essential to ensure precise bioprocess control. This review presents different types of bioreactors, sensors, and applications in other sectors. Bioreactors, controlled systems for cultivating microorganisms and cells, are essential tools in various fields, from scientific research to industrial production. The use of a variety of sensors is critical for accurate, real-time monitoring, early problem detection, reproducibility, cost reduction, and increased efficiency. These benefits are being realized in numerous applications, including biofuel production, bioremediation and leaching processes, tissue engineering, and drug manufacturing. Innovations in bioreactor technology are expanding opportunities for a more sustainable and healthier future. By developing new types of bioreactors, integrating advanced sensors, and exploring promising applications, bioreactors are playing a key role in addressing global challenges and sustainably advancing science and technology.
Cytotoxicity, structural, conformational, and techno-functional properties of α-lactalbumin nanostructured aggregates Jhonatan Rafael de Oliveira Bianchi, Bruna Elisa Reis Paz, Larissa Germano Fonseca, Rebeca Lima Carvalho, José Carlos Magalhães, Karina Azevedo Pacheco, Andersen Escobar Schlogl, Daniela Leite Fabrino, Edson Romano Nucci, Jane Sélia dos Reis Coimbra, Igor José Boggione Santos Journal of Food Science, 2023 Protein nanostructures can be used in food applications to improve the techno‐functional properties of a food formulation. This study aims to find the best conditions for the production and conformational change of α‐lactalbumin nanostructured aggregates. The criteria to determine the best operating conditions to produce α‐lactalbumin nanostructured aggregates were intensification of foaming and emulsification, techno‐functional proprieties, cytotoxic, and antibacterial activity of nanostructures compared with native α‐lactalbumin. Conformational alterations occurred in the α‐helix and sheet‐β protein structures. The size obtained by dynamic light scattering was 163.84 nm with a polydispersity index of 0.29. The nano protein improved the techno‐functional property compared to the native protein. Additionally, nanostructures had no cytotoxic effect and were innocuous to bacterial activity. Thus, this study presents the best conditions to produce α‐lactalbumin nanostructured aggregates with improved properties that allow new food industry applications.
Lactoferrin, chitosan and Melaleuca alternifolia—natural products that show promise in candidiasis treatment Lorena de Oliveira Felipe, Willer Ferreira da Silva Júnior, Katialaine Corrêa de Araújo, Daniela Leite Fabrino Brazilian Journal of Microbiology, 2018 The evolution of microorganisms resistant to many medicines has become a major challenge for the scientific community around the world. Motivated by the gravity of such a situation, the World Health Organization released a report in 2014 with the aim of providing updated information on this critical scenario. Among the most worrying microorganisms, species from the genus Candida have exhibited a high rate of resistance to antifungal drugs. Therefore, the objective of this review is to show that the use of natural products (extracts or isolated biomolecules), along with conventional antifungal therapy, can be a very promising strategy to overcome microbial multiresistance. Some promising alternatives are essential oils of Melaleuca alternifolia (mainly composed of terpinen-4-ol, a type of monoterpene), lactoferrin (a peptide isolated from milk) and chitosan (a copolymer from chitin). Such products have great potential to increase antifungal therapy efficacy, mitigate side effects and provide a wide range of action in antifungal therapy.
Nitric oxide and trypanosomatids: A tale of Dr. Jekyll and Mr. Hyde Nitric Oxide Emerging Developments Therapeutic Role in Disease States and Health Effects, 2015
L-arginine metabolism in the pathogenic trypanosomatids trypanosoma cruzi and leishmania sp. Nitric Oxide Emerging Developments Therapeutic Role in Disease States and Health Effects, 2015
Cells and mediators of inflammation (C-reactive protein, nitric oxide, platelets and neutrophils) in the acute and convalescent phases of uncomplicated Plasmodium vivax and Plasmodium falciparum infection Josué da Costa Lima-Junior, Rodrigo Nunes Rodrigues-da-Silva, Virgínia Araújo Pereira, Fábio Luiz Storer, Daiana de Souza Perce-da-Silva, Daniela Leite Fabrino, Fátima Santos, Dalma Maria Banic, Joseli de Oliveira-Ferreira Memorias do Instituto Oswaldo Cruz, 2012 The haematological changes and release of soluble mediators, particularly C-reactive protein (CRP) and nitric oxide (NO), during uncomplicated malaria have not been well studied, especially in Brazilian areas in which the disease is endemic. Therefore, the present study examined these factors in acute (day 0) and convalescent phase (day 15) patients infected with Plasmodium falciparum and Plasmodium vivax malaria in the Brazilian Amazon. Haematologic parameters were measured using automated cell counting, CRP levels were measured with ELISA and NO plasma levels were measured by the Griess reaction. Our data indicate that individuals with uncomplicated P. vivax and P. falciparum infection presented similar inflammatory profiles with respect to white blood cells, with high band cell production and a considerable degree of thrombocytopaenia during the acute phase of infection. Higher CRP levels were detected in acute P. vivax infection than in acute P. falciparum infection, while higher NO was detected in patients with acute and convalescent P. falciparum infections. Although changes in these mediators cannot predict malaria infection, the haematological aspects associated with malaria infection, especially the roles of platelets and band cells, need to be investigated further.
Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infection Daniela L. Fabrino, Grazielle A. Ribeiro, Lívia Teixeira, Rossana C.N. Melo Methods in Molecular Biology, 2011 During acute infection with the parasite Trypanosoma cruzi, the causal agent of Chagas' disease, tissue damage is related to intense tissue parasitism. Here we discuss histological approaches for an optimal visualization and quantification of T. cruzi nests in the heart, the main target organ of the parasite. These analyses are important to evaluate the course of the infection in different experimental models and also can be used to investigate parasite colonization and inflammatory processes in other infected tissues and biopsies.
Porins facilitate nitric oxide-mediated killing of mycobacteria Daniela Leite Fabrino, Christopher K.E. Bleck, Elsa Anes, Andrej Hasilik, Rossana C.N. Melo, Michael Niederweis, Gareth Griffiths, Maximiliano Gabriel Gutierrez Microbes and Infection, 2009
CuO nanoparticles/polymer nanocomposite hydrogels ILP Lopes, PA Ribeiro, EVB da Fonseca, AE Schlogl, BH Costa, ... Hydrogels for Wound Healing, 271-288 , 2026 2026
Self-Assembled Α-Lactalbumin Nanostructures: Encapsulation and Controlled Release of Bioactive in Gastrointestinal in Vitro Model J Rafael de Oliveira Bianchi, DL Fabrino, CMF Quintão, ... Daniela Leite and Quintão, Cristiane Medina Finzi and dos Reis Coimbra, Jane … , 2025 2025
Self‐assembled α‐lactalbumin nanostructures: encapsulation and controlled release of bioactive molecules in gastrointestinal in vitro model JR de Oliveira Bianchi, DL Fabrino, CMF Quintão, JS dos Reis Coimbra, ... Journal of the Science of Food and Agriculture 104 (15), 9592-9602 , 2024 2024
Bioreactors: applications and innovations for a sustainable and healthy future—a critical review F Palladino, PRF Marcelino, AE Schlogl, ÁHM José, RCLB Rodrigues, ... Applied Sciences 14 (20), 9346 , 2024 2024 Citations: 113
Self-assembled⊍-lactalbumin nanostructures: encapsulation and controlled release of bioactive molecules in gastrointestinal JR de Oliveira Bianchi, DL Fabrino, CMF Quintão, JS dos Reis Coimbrac, ... 2024
Cytotoxicity, structural, conformational, and techno‐functional properties of α‐lactalbumin nanostructured aggregates JRO Bianchi, BER Paz, LG Fonseca, RL Carvalho, JC Magalhães, ... Journal of Food Science 88 (7), 3049-3062 , 2023 2023 Citations: 5
Prospects and applications of nanotechnology for boosting the efficacy and stability of green products in the food system IJB Santos, MM Gonçalves, DL Fabrino, PA Ribeiro, JS dos Reis Coimbra Green Products in Food Safety, 277-308 , 2023 2023
Cell viability evaluation of vero cells viability cultured on different chitosan films: development of functional biodressings possibilities VC Leite, WW Pereira Filho, EN de Oliveira Junior, D Fabrino International Journal of Advances in Medical Biotechnology-IJAMB 3 (1), 34-39 , 2020 2020 Citations: 1
Lactoferrin, chitosan and Melaleuca alternifolia—natural products that show promise in candidiasis treatment L de Oliveira Felipe, WF da Silva Júnior, KC de Araújo, DL Fabrino Brazilian journal of microbiology 49 (2), 212-219 , 2018 2018 Citations: 48
Development of scaffolds based on chitosan, type I collagen and hyaluronic acid as biomaterial for three-dimensional cell culture L Rabello, V Trindade, E Oliveira, DL Fabrino Proceedings of the 14◦ Congresso da Sociedade Latino Americana de … , 2017 2017 Citations: 2
Lactoferrin, chitosan and Melaleuca alternifolia-natural products that show promise in candidiasis treatment DL FABRINO Brazilian journal of microbiology:[publication of the Brazilian Society for … , 2017 2017
Xadrez: uma ferramenta para aprimorar a mente humana JPL de Miranda, IAPA de São, JPGR Nunes, PC Lauar, JE Ottoni, ... Revista Extensão em Foco, 37-47 , 2017 2017
Three-dimensional cell culture, opportunities and challenges for bioprocess engineers SH Pereira, DSM Coutinho, AFOG Matos, WF Silva Junior, DL Fabrino Brazilian Journal of Biological Sciences 3 (6), 263-277 , 2016 2016 Citations: 8
Análise do nível de conhecimento e motivação de alunos do ensino médio rumo ao ensino superior: Projeto Diálogos sobre o que Significa Cursar Engenharia TGV NASCIMENTO, ACC Pena, A Silva, SL Sales, RA Pícoli, DL Fabrino Revista Brasileira de Extensão Universitária 6 (1), 7-13 , 2015 2015 Citations: 7
A Importância de Radicais Livres Durante a Infecção por Protozoários do Gênero Leishmania DB Castro-Pinto, DL Fabrino, L Leon 2014 Citations: 1
Cells and mediators of inflammation (C-reactive protein, nitric oxide, platelets and neutrophils) in the acute and convalescent phases of uncomplicated Plasmodium vivax and … JC Lima-Junior, RN Rodrigues-da-Silva, VA Pereira, FL Storer, ... Memórias do Instituto Oswaldo Cruz 107, 1035-1041 , 2012 2012 Citations: 42
Rat Models to Investigate Host Macrophage Defense against Trypanosoma cruzi DL Fabrino, LL Leon, M Genestra, GG Parreira, RCN Melo Journal of innate immunity 3 (1), 71-82 , 2010 2010 Citations: 12
Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infection DL Fabrino, GA Ribeiro, L Teixeira, RCN Melo Light Microscopy: Methods and Protocols, 69-80 , 2010 2010 Citations: 4
Porins facilitate nitric oxide-mediated killing of mycobacteria DL Fabrino, CKE Bleck, E Anes, A Hasilik, RCN Melo, M Niederweis, ... Microbes and infection 11 (10-11), 868-875 , 2009 2009 Citations: 30
In vitro sodium nitroprusside‐mediated toxicity towards Leishmania amazonensis promastigotes and axenic amastigotes M Genestra, RJ Soares‐Bezerra, L Gomes‐Silva, DL Fabrino, ... Cell Biochemistry and Function: Cellular biochemistry and its modulation by … , 2008 2008 Citations: 22
MOST CITED SCHOLAR PUBLICATIONS
Macrophage lipid body induction by Chagas disease in vivo: putative intracellular domains for eicosanoid formation during infection RCN Melo, H D’Ávila, DL Fabrino, PE Almeida, PT Bozza Tissue and cell 35 (1), 59-67 , 2003 2003 Citations: 138
Bioreactors: applications and innovations for a sustainable and healthy future—a critical review F Palladino, PRF Marcelino, AE Schlogl, ÁHM José, RCLB Rodrigues, ... Applied Sciences 14 (20), 9346 , 2024 2024 Citations: 113
Lipid bodies: structural markers of inflammatory macrophages in innate immunity RCN Melo, DL Fabrino, FF Dias, GG Parreira Inflammation Research 55 (8), 342-348 , 2006 2006 Citations: 84
Production of hydrogen peroxide by peripheral blood monocytes and specific macrophages during experimental infection with Trypanosoma cruzi in vivo RCN Melo, DL Fabrino, H D'Ávila, HC Teixeira, AP Ferreira Cell Biology International 27 (10), 853-861 , 2003 2003 Citations: 67
Lactoferrin, chitosan and Melaleuca alternifolia—natural products that show promise in candidiasis treatment L de Oliveira Felipe, WF da Silva Júnior, KC de Araújo, DL Fabrino Brazilian journal of microbiology 49 (2), 212-219 , 2018 2018 Citations: 48
Cells and mediators of inflammation (C-reactive protein, nitric oxide, platelets and neutrophils) in the acute and convalescent phases of uncomplicated Plasmodium vivax and … JC Lima-Junior, RN Rodrigues-da-Silva, VA Pereira, FL Storer, ... Memórias do Instituto Oswaldo Cruz 107, 1035-1041 , 2012 2012 Citations: 42
Porins facilitate nitric oxide-mediated killing of mycobacteria DL Fabrino, CKE Bleck, E Anes, A Hasilik, RCN Melo, M Niederweis, ... Microbes and infection 11 (10-11), 868-875 , 2009 2009 Citations: 30
Peripheral blood monocytes show morphological pattern of activation and decreased nitric oxide production during acute Chagas’ disease in rats DL Fabrino, LL Leon, GG Parreira, M Genestra, PE Almeida, RCN Melo Nitric Oxide 11 (2), 166-174 , 2004 2004 Citations: 28
In vitro sodium nitroprusside‐mediated toxicity towards Leishmania amazonensis promastigotes and axenic amastigotes M Genestra, RJ Soares‐Bezerra, L Gomes‐Silva, DL Fabrino, ... Cell Biochemistry and Function: Cellular biochemistry and its modulation by … , 2008 2008 Citations: 22
Rat Models to Investigate Host Macrophage Defense against Trypanosoma cruzi DL Fabrino, LL Leon, M Genestra, GG Parreira, RCN Melo Journal of innate immunity 3 (1), 71-82 , 2010 2010 Citations: 12
Three-dimensional cell culture, opportunities and challenges for bioprocess engineers SH Pereira, DSM Coutinho, AFOG Matos, WF Silva Junior, DL Fabrino Brazilian Journal of Biological Sciences 3 (6), 263-277 , 2016 2016 Citations: 8
Análise do nível de conhecimento e motivação de alunos do ensino médio rumo ao ensino superior: Projeto Diálogos sobre o que Significa Cursar Engenharia TGV NASCIMENTO, ACC Pena, A Silva, SL Sales, RA Pícoli, DL Fabrino Revista Brasileira de Extensão Universitária 6 (1), 7-13 , 2015 2015 Citations: 7
Cytotoxicity, structural, conformational, and techno‐functional properties of α‐lactalbumin nanostructured aggregates JRO Bianchi, BER Paz, LG Fonseca, RL Carvalho, JC Magalhães, ... Journal of Food Science 88 (7), 3049-3062 , 2023 2023 Citations: 5
Histological Approaches to Study Tissue Parasitism During the Experimental Trypanosoma cruzi Infection DL Fabrino, GA Ribeiro, L Teixeira, RCN Melo Light Microscopy: Methods and Protocols, 69-80 , 2010 2010 Citations: 4
Development of scaffolds based on chitosan, type I collagen and hyaluronic acid as biomaterial for three-dimensional cell culture L Rabello, V Trindade, E Oliveira, DL Fabrino Proceedings of the 14◦ Congresso da Sociedade Latino Americana de … , 2017 2017 Citations: 2
Cell viability evaluation of vero cells viability cultured on different chitosan films: development of functional biodressings possibilities VC Leite, WW Pereira Filho, EN de Oliveira Junior, D Fabrino International Journal of Advances in Medical Biotechnology-IJAMB 3 (1), 34-39 , 2020 2020 Citations: 1
A Importância de Radicais Livres Durante a Infecção por Protozoários do Gênero Leishmania DB Castro-Pinto, DL Fabrino, L Leon 2014 Citations: 1
CuO nanoparticles/polymer nanocomposite hydrogels ILP Lopes, PA Ribeiro, EVB da Fonseca, AE Schlogl, BH Costa, ... Hydrogels for Wound Healing, 271-288 , 2026 2026
Self-Assembled Α-Lactalbumin Nanostructures: Encapsulation and Controlled Release of Bioactive in Gastrointestinal in Vitro Model J Rafael de Oliveira Bianchi, DL Fabrino, CMF Quintão, ... Daniela Leite and Quintão, Cristiane Medina Finzi and dos Reis Coimbra, Jane … , 2025 2025
Self‐assembled α‐lactalbumin nanostructures: encapsulation and controlled release of bioactive molecules in gastrointestinal in vitro model JR de Oliveira Bianchi, DL Fabrino, CMF Quintão, JS dos Reis Coimbra, ... Journal of the Science of Food and Agriculture 104 (15), 9592-9602 , 2024 2024
