Effect of microencapsulation on antioxidant activities of Eugenia punicifolia (Kunth) DC hydroethanolic extracts MANOELA MARTINS, DANIJELA STANISIC, CATARINA DOS SANTOS Anais Da Academia Brasileira De Ciencias, 2024 Eugenia punicifolia (Kunth) DC (Myrtaceae) is a folk medicinal plant in the Brazilian Cerrado with antioxidant, anti-inflammatory, antinociceptive, antiulcerogenic activities, etc., usually attributed to its phenolic compounds. Since these compounds are sensitive to heat and light, and to increase their applications, Hydroethanolic Extracts E. punicifolia (HEEP, EtOH:H2O 70% v/v) were encapsulated by freeze-drying in xanthan gum (mesh 80, HEEPX80; mesh 200, HEEPX200) in ratio 1:1(w/w). Flavonoids had the highest encapsulation efficiency in HEEPX80, with a total flavonoid content of 55.56%. The release profile at different pH levels showed that pH = 4.5, a relevant antioxidant activity for HEEPX80 and HEEPX200. Also, in HEEP-modified release, higher antioxidant activity was observed in more acidic media (pH 4.5) than in a more neutral medium (pH 7.4). From these results, we could infer that HEEP encapsulations with Xanthan gum could be a good alternative for preserving antioxidants in these extracts.
Optimization of Eugenia punicifolia (Kunth) D. C. leaf extraction using a simplex centroid design focused on extracting phenolics with antioxidant and antiproliferative activities Catarina dos Santos, Andressa Lie Mizobucchi, Bruna Escaramboni, Bruno Pereira Lopes, Celio Fernando Figueiredo Angolini, Marcos Nogueira Eberlin, Karina Alves de Toledo, Eutimio Gustavo Fernández Núñez BMC Chemistry, 2020 Eugenia punicifolia (Kunth) D. C. (Myrtaceae) has been showing interesting biological activities in the literature which was correlated to its phenolic compounds. In the sense of a better recovering of phenolics with the best antioxidant and antiproliferative activities, an extraction, based on multivariate analytical approach, was developed from E. punicifolia leaves. The different extractor solvents (ethanol, methanol and water) and their binary and ternary combinations were evaluated using a simplex-centroid mixture design and surface response methodology. The optimized crude extracts were investigated for phenol and flavonoid content and compared to their antioxidant (EC50) and antiproliferative properties against HEp-2 (cell line derived from the oropharyngeal carcinoma) and mononuclear viability cells. Ethanolic extracts showed the best phenolic content with the highest antioxidant activity and moderated activity antiproliferative to HEp-2. ESI-QTOF–MS revealed the presence of quercetin and myricetin derivatives, which was correlated to activities tested. Then, simplex-centroid design allowed us to correlate the Eugenia punicifolia biological activities with the extracts obtained from solvent different polarity mixtures.
Sex-specific effects of Eugenia punicifolia extract on gastric ulcer healing in rats Larissa Lucena Périco, Vinícius Peixoto Rodrigues, Rie Ohara, Gabriela Bueno, Vânia Vasti Alfieri Nunes, Raquel de Cássia dos Santos, Ana Carolina Lima Camargo, Luis Antônio Justulin Júnior, Sérgio Faloni de Andrade, Viviane Miranda Bispo Steimbach, Luísa Mota da Silva, Lúcia Regina Machado da Rocha, Wagner Vilegas, Catarina dos Santos, Clélia Akiko Hiruma-Lima World Journal of Gastroenterology, 2018 AIM To evaluate the sex-specific effects of a hydroalcoholic extract from Eugenia punicifolia (HEEP) leaves on gastric ulcer healing. METHODS In this rat study involving males, intact (cycling) females, and ovariectomized females, gastric ulcers were induced using acetic acid. A vehicle, lansoprazole, or HEEP was administered for 14 d after ulcer induction. Body weight was monitored throughout the treatment period. At the end of treatment, the rats were euthanized and the following in vivo and in vitro investigations were performed: macroscopic examination of the lesion area and organ weights, biochemical analysis, zymography, and evaluation of protein expression levels. Additionally, the concentration-dependent effect of HEEP was evaluated in terms of subacute toxicity and cytotoxicity. RESULTS Compared to the vehicle, HEEP demonstrated a great healing capacity by substantially reducing the ulcerative lesion area in males (52.44%), intact females (85.22%), and ovariectomized females (65.47%), confirming that HEEP accelerates the healing of acetic acid-induced gastric lesions and suggesting that this effect is modulated by female sex hormones. The antiulcer effect of HEEP was mediated by prostaglandin E2 only in male rats. Overall, the beneficial effect of HEEP was the highest in intact females. Notably, HEEP promoted the expression of vascular endothelial growth factor (intact vs ovariectomized females) and decreased the expression of Caspase-8 and Bcl-2 (intact female vs male or ovariectomized female). Additionally, HEEP enhanced fibroblast proliferation and migration into a wounded area in vitro, confirming its healing effect. Finally, no sign of subacute toxicity or cytotoxicity of HEEP was observed. CONCLUSION In gastric ulcers, HEEP-induced healing (modulated by female sex hormones; in males, mediated by prostaglandin) involves extracellular matrix remodeling, with gastric mucosa cell proliferation and migration.
Eugenia aurata and Eugenia punicifolia HBK inhibit inflammatory response by reducing neutrophil adhesion, degranulation and NET release Mírian Feliciano Costa, Tais Iara Jesus, Bruno Rafael Pereira Lopes, Célio Fernando Figueiredo Angolini, Abner Montagnolli, Lorraine de Paula Gomes, Gabriela Sterle Pereira, Ana Lucia Tasca Gois Ruiz, João Ernesto Carvalho, Marcos Nogueira Eberlin, Catarina dos Santos, Karina Alves Toledo BMC Complementary and Alternative Medicine, 2016 BACKGROUND: Eugenia spp. are used in popular medicine in the treatment of pain, diabetes, intestinal disorders and cough. The aim of the work is to evaluate, ex vivo and in vivo, the anti-inflammatory activity of the hydroethanolic extracts of the leaves of Eugenia aurata (EA) and Eugenia punicifolia HBK (EP) upon neutrophils. METHODS: Ex vivo, isolated human neutrophils were sensitized by Eugenia extracts (0.1-1000 μg/mL) and stimulated by PMA. In these conditions, different neutrophil activities related to inflammatory process were measured: adhesion, degranulation and NET release. Neutrophil viability and tumor line cells were monitored. In vivo, neutrophil influx was evaluated by peritonitis model performed in mice pretreated with different concentrations of Eugenia extracts. Phytochemical profile was assessed by mass spectrometry. RESULTS: Ex vivo, EA and EP (1000 μg/mL) reduced cell adhesion and degranulation, respectively. NET release was inhibited by EA and EP. Anti-inflammatory activities occurred in the absence of cytotoxicity. In vivo, both EA as EP inhibited neutrophil migration. The phytochemical profile revealed that EA contains myricitrin, rutin, quinic acid and quercetin derivatives. EP presents gallic acid, quercetin derivatives, syringic acid, ellagic acid, monogalloyl-glucose, glycosyringic acid, mudanoside B, HHDP glucose isomer and digalloylglucose isomer. EA and EP inhibit neutrophil migration by different pathways. CONCLUSION: Different chemical compositions may explain the anti-inflammatory effects described herein for EA and EP. Both extracts inhibit NET release but only EA reduces cell adhesion whereas EP decreases elastase secretion. This work contributes to the elucidation of cellular mechanisms related to the anti-inflammatory activity for leaves of E. aurata and E. punicifolia HBK.
Novel sustained-release of stryphnodendron obovatum leaves extract using natural rubber latex as carrier Revista De Ciencias Farmaceuticas Basica E Aplicada, 2015
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Antinociceptive, anti-inflammatory and gastroprotective effects of a hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC. in rodents. RT Basting, CM Nishijima, JA Lopes, RC Santos, LL Périco, S Laufer, ... Journal of Ethnopharmacology. 157, 257-267 , 2014 2014 Citations: 55
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MOST CITED SCHOLAR PUBLICATIONS
Microencapsulation of an anthocyanin-rich blackberry (Rubus spp.) by-product extract by freeze-drying C Yamashita, MMS Chung, C dos Santos, CRM Mayer, ICF Moraes, ... Lwt 84, 256-262 , 2017 2017 Citations: 266
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Antinociceptive, anti-inflammatory and gastroprotective effects of a hydroalcoholic extract from the leaves of Eugenia punicifolia (Kunth) DC. in rodents. RT Basting, CM Nishijima, JA Lopes, RC Santos, LL Périco, S Laufer, ... Journal of Ethnopharmacology. 157, 257-267 , 2014 2014 Citations: 55
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Novel sustained-release of Stryphnodendron obovatum leaves extract using natural rubber latex as carrier. FA Borges, PR Siguematsu, RD Herculano, C Dos Santos Journal of Basic & Applied Pharmaceutical Sciences/Revista de Ciências … , 2015 2015 Citations: 9
Role of the antioxidant pathway in the healing of peptic ulcers induced by ischemia–reperfusion in male and female rats treated with Eugenia punicifolia L Lucena Périco, R de Cássia dos Santos, V Peixoto Rodrigues, ... Inflammopharmacology 30 (4), 1383-1394 , 2022 2022 Citations: 8
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