Chemical composition and toxicity of Hedyosmum brasiliense essential oil against storage pests and fungi Rubens Candido Zimmermann, Carolina Gracia Poitevin, Ana Marta Schafaschek, Milena Ielen, Sofia Bin Macedo, Wanderlei do Amaral, Greissi Tente Giraldi, Edson José Mazarotto, Alessandra Benatto, Carlos Eduardo Nogueira Martins, Beatriz Helena L.N. Sales Maia, Elaine Fernanda Dos Santos, Lino Bittencourt Monteiro, Joatan Machado da Rosa, Henrique da Silva Silveira Duarte Journal of Stored Products Research, 2025 Species of Hedyosmum , widely distributed across Latin America, synthesize and store essential oils (EOs) with diverse biological activities. However, studies on the agricultural applications of some species within this genus remain limited. This study evaluated the biological activities of Hedyosmum brasiliense EO (HBEO) against storage pests and fungi, alongside its cytotoxicity in human cells. HBEO was extracted from leaves using steam distillation, and its chemical composition was determined via gas chromatography-mass spectrometry (GC-MS). Insecticidal activity against Sitophilus oryzae and Sitophilus zeamais was assessed using contact and fumigation methods, with biochemical markers assessed under fumigation. The fungicidal activity was tested through volatilization and contact against Aspergillus flavus , Aspergillus niger , Aspergillus nomius , Fusarium culmorum , and Fusarium graminearum . Cytotoxicity was analyzed using the resazurin assay in human fibroblast cultures following exposure to HBEO by volatilization and contact. GC-MS analysis identified 28 compounds, accounting for 91.45 % of the total chemical composition, with sabinene and bicyclogermacrene as the major constituents. Fumigation was the most effective method for controlling weevils, achieving approximately 49 % mortality. HBEO altered acetylcholinesterase, glutathione S-transferase, and superoxide dismutase in S. oryzae and disrupted esterases while inducing lipid peroxidation in S. zeamais . Fungicidal assays revealed that HBEO inhibited the mycelial growth of Fusarium isolates, with the contact method achieving inhibition rates exceeding 50 %. No fibroblast growth was verified after exposure to HBEO by contact and volatilization at all concentrations evaluated. While HBEO demonstrated promising insecticidal and fungicidal properties against storage pests and fungi, its cytotoxicity to human cells at the tested concentrations renders it unsuitable for direct commercial application in its current form. • First report of insecticidal, antifungal and cytotoxic of Hedyosmum brasiliense essential oil. • Hedyosmum brasiliense EO demonstrated greater mortality for Sitophilus oryzae and S. zeamais via fumigation. • Hedyosmum brasiliense EO caused changes in biochemical markers for Sitophilus species. • Hedyosmum brasiliense EO inhibited the mycelial growth of Fusarium isolates. • Hedyosmum brasiliense EO exhibited cytotoxic effects on human cells.
Finishing systems on the carcass and meat characteristics and fatty acid profile of the longissimus thoracis muscle of Hereford steers in southern Brazil Vanessa Peripolli, Grace Adele Gadotti, Carlos Eduardo Nogueira Martins, Betina Raquel Cunha dos Santos, Ivan Bianchi, Fabiana Moreira, Elizabeth Schwegler, Maiko Giorgi Philippe, Andressa Rozzetto Garcia, Marcílio Félix, José Laerte Nörnberg Applied Food Research, 2025 • Finishing systems affect carcass and meat characteristics in Hereford steers in southern Brazil. • Pasture-finished Hereford steers show a beneficial fatty acid profile and meat traits. • Supplementing pasture-fed Hereford steers yields a fatty acid profile close to pasture-fed. • Premium-quality Hereford beef is achievable with pasture systems in regional settings. • Premium-quality Hereford beef is possible with pasture systems in specific regions. This study aimed to evaluate the effect of finishing systems on the carcass and meat characteristics and fatty acid profile of the longissimus thoracis (LT) muscle of Hereford steers in southern Brazil. Thirty-six carcasses were chosen from animals aged 18-24 months, weighing at least 220kg, with a minimum fat thickness of 3mm, finished on pasture (n=12), pasture with concentrate supplementation (n=12), and feedlot (n=12). Evaluated parameters included age, fat thickness, pH, color, marbling, and fatty acid composition. Correlations were found between meat color and pH (r=0.43), marbling and fat thickness (r=0.38), and age and fat color (r=0.98). The principal component analysis explained 52.52 % of the variation. Finishing systems significantly influenced carcass characteristics and meat color (P<0.05) but not pH or fat thickness (P>0.05) of Hereford steers. Chemical composition and fatty acid profile varied with the finishing system (P<0.05), except for total lipids and certain fatty acids (P>0.05). Pasture-finished Hereford steers exhibited distinct characteristics compared to feedlot-finished, with higher omega-3, C18:0, C18:1n9c, trans-11 C18:1n7, 18:3n3, CLA-18:2n7 cis-9 trans-11, EPA-C20:1n9, C20:4n6, C20:5n3, MUFA, and desirable fatty acids, along with darker meat and fat. Pasture-finished Hereford steer meats. Meat from pasture-finished Hereford steer selected based on premium-quality carcass and meat characteristics, reared under southern Brazilian conditions, shows potential to meet niche market demands.
Toxicity of Melaleuca rhaphiophylla essential oils against Sitophilus zeamais and Sitophilus oryzae Rubens Candido Zimmermann, Ana Marta Schafaschek, Adélia Maria Bischoff, Matheus Beger, Julia Sant’ana, Sofia Bin de Macedo, Milena Ielen, Carlos Eduardo Nogueira Martins, Sarah da Costa Amaral, Rilton Alves de Freitas, Alessandra Benatto, Carolina Gracia Poitevin, Henrique da Silva Silveira Duarte, Joatan Machado da Rosa Journal of Pest Science, 2025
Kernel Density Estimation (KDE) as a tool to enhance bovine tuberculosis surveillance in Santa Catarina, Brazil Luiz Felipe Crispim Lourenço, Frederico Monfardini, Carlos Eduardo Nogueira Martins, Ricardo Evandro Mendes Revista Brasileira De Medicina Veterinaria, 2025 In areas with low bovine tuberculosis (bTB) prevalence, such as Santa Catarina state, Brazil, effective surveillance is essential for disease eradication. Current strategies may miss high-risk farms by inadequately considering spatial risk factors. This study used Kernel Density Estimation (KDE) to analyze spatial risk patterns in Santa Catarina, Brazil, leveraging the official veterinary service's (CIDASC's Sigen+ database) farm data, testing history, and animal movement records. Results revealed that while existing surveillance targets many high-risk areas, some remain unmonitored. Practices such as on-farm slaughter and insufficient movement testing create vulnerabilities that can hinder bTB detection. Integrating KDE-derived risk maps into the current surveillance efforts can improve targeted resource allocation and disease control. This study demonstrated the value of spatial risk analysis for enhancing bTB surveillance in Santa Catarina state, offering a strategic tool to support CIDASC's eradication efforts and serving as a model for other regions seeking to strengthen their surveillance programs.
Antifungal activity of essential oils and their combinations against storage fungi Rubens Candido Zimmermann, Carolina Gracia Poitevin, Thaisa Siqueira da Luz, Edson José Mazarotto, Jason Lee Furuie, Carlos Eduardo Nogueira Martins, Wanderlei do Amaral, Roger Raupp Cipriano, Joatan Machado da Rosa, Ida Chapaval Pimentel, Maria A. C. Zawadneak Environmental Science and Pollution Research, 2023
Animal performance in oat and Italian ryegrass pastures under leaf lamina biomass levels Duilio Guerra Bandinelli, Fernando Luiz Ferreira de Quadros, Adriano Rudi Maixner, Luiz Felipe Cattani Simões, Carlos Eduardo Nogueira Martins, Alexandre Coradini Fontoura da Silva, Naíme de Barcellos Trevisan, Marcos da Silva Brum, Niumar Dutra Aurélio Pesquisa Agropecuaria Brasileira, 2005
