Oral health of an indigenous population in northeastern Brazil: a cross-sectional Study of the Fulni-ô ethnic group Bruna Del Vechio Koike, Rosangela Maria Pereira Valões, Claudia Cazal, Vanessa Cardoso Pereira, Carlos Alberto de Carvalho Fraga, et al. Sao Paulo Medical Journal, 2024 BACKGROUND: There is a lack of studies evaluating the oral health of traditional indigenous communities in Brazil. OBJECTIVES: Thus, the objective of this study was to describe the oral health characteristics of the indigenous Fulni-ô ethnic group in Northeast Brazil. DESIGN AND SETTING: A cross-sectional observational investigation was conducted within the Project on Atherosclerosis among Indigenous Populations. METHODS: This study included participants of both sexes from the Fulni-ô ethnic group. The participants included in this investigation underwent a comprehensive oral health evaluation by a registered and experienced dentist to assess oral health and identify potentially malignant oral lesions. Participants with suspicious lesions were referred for biopsy. Shapiro-Wilk, Mann-Whitney, and Student’s t-tests were used, and measures of central tendency and dispersion were described. Statistical significance was 5%. RESULTS: A total of 104 individuals were included in this study. The prevalence of the use of tobacco derivatives was 94.0%, with similarities between sexes. The prevalence of oral changes in this study population was 84.4%. Fifty-one individuals who underwent oral reassessment were referred for oral lesion biopsy. CONCLUSIONS: This study demonstrated a high prevalence of oral alterations in the Fulni-ô population. Histopathological analyses indicated the presence of mild oral epithelial dysplasia in five cases.
Hippocampus-retrosplenial cortex interaction is increased during phasic REM and contributes to memory consolidation Daniel Gomes de Almeida-Filho, Bruna Del Vechio Koike, Francesca Billwiller, Kelly Soares Farias, Igor Rafael Praxedes de Sales, et al. Scientific Reports, 2021 Hippocampal (HPC) theta oscillation during post-training rapid eye movement (REM) sleep supports spatial learning. Theta also modulates neuronal and oscillatory activity in the retrosplenial cortex (RSC) during REM sleep. To investigate the relevance of theta-driven interaction between these two regions to memory consolidation, we computed the Granger causality within theta range on electrophysiological data recorded in freely behaving rats during REM sleep, both before and after contextual fear conditioning. We found a training-induced modulation of causality between HPC and RSC that was correlated with memory retrieval 24 h later. Retrieval was proportional to the change in the relative influence RSC exerted upon HPC theta oscillation. Importantly, causality peaked during theta acceleration, in synchrony with phasic REM sleep. Altogether, these results support a role for phasic REM sleep in hippocampo-cortical memory consolidation and suggest that causality modulation between RSC and HPC during REM sleep plays a functional role in that phenomenon.
Forced internal desynchrony induces cardiometabolic alterations in adult rats Isis Gabrielli Barbieri de Oliveira, Marcos Divino Ferreira Junior, Paulo Ricardo Lopes, Dhiogenes Balsanufo Taveira Campos, Marcos Luiz Ferreira-Neto, et al. Journal of Endocrinology, 2019 Disruptions in circadian rhythms have been associated with several diseases, including cardiovascular and metabolic disorders. Forced internal desynchronization induced by a period of T-cycles of 22 h (T22 protocol) reaches the lower limit of entrainment and dissociates the circadian rhythmicity of the locomotor activity into two components, driven by different outputs from the suprachiasmatic nucleus (SCN). The main goal of this study was to evaluate the cardiovascular and metabolic response in rats submitted to internal desynchronization by T22 protocol. Male Wistar rats were assigned to either a control group subjected to a usual T-cycles of 24 h (12 h–12 h) or an experimental group subjected to the T22 protocol involving a 22-h symmetric light–dark cycle (11 h–11 h). After 8 weeks, rats subjected to the T22 exhibited desynchrony in their locomotor activity. Although plasma glucose and insulin levels were similar in both groups, desynchronized rats demonstrated dyslipidemia, significant hypertrophy of the fasciculate zone of the adrenal gland, low IRB, IRS2, PI3K, AKT, SOD and CAT protein expression and an increased expression of phosphoenolpyruvate carboxykinase in the liver. Furthermore, though they maintained normal baseline heart rates and mean arterial pressure levels, they also presented reduced baroreflex sensitivity. The findings indicate that circadian timing desynchrony following the T22 protocol can induce cardiometabolic disruptions. Early hepatic metabolism dysfunction can trigger other disorders, though additional studies are needed to clarify the causes.
Rhythms of core clock genes and spontaneous locomotor activity in post-status Epilepticus model of mesial temporal lobe epilepsy Heloisa de Carvalho Matos, Bruna Del Vechio Koike, Wanessa dos Santos Pereira, Tiago G. de Andrade, Olagide W. Castro, et al. Frontiers in Neurology, 2018 The interaction of Mesial Temporal Lobe Epilepsy (mTLE) with the circadian system control is apparent from an oscillatory pattern of limbic seizures, daytime's effect on seizure onset and the efficacy of antiepileptic drugs. Moreover, seizures per se can interfere with the biological rhythm output, including circadian oscillation of body temperature, locomotor activity, EEG pattern as well as the transcriptome. However, the molecular mechanisms underlying this cross-talk remain unclear. In this study, we systematically evaluated the temporal expression of seven core circadian transcripts (Bmal1, Clock, Cry1, Cry2, Per1, Per2, and Per3) and the spontaneous locomotor activity (SLA) in post-status epilepticus (SE) model of mTLE. Twenty-four hour oscillating SLA remained intact in post-SE groups although the circadian phase and the amount and intensity of activity were changed in early post-SE and epileptic phases. The acrophase of the SLA rhythm was delayed during epileptogenesis, a fragmented 24 h rhythmicity and extended active phase length appeared in the epileptic phase. The temporal expression of circadian transcripts Bmal1, Cry1, Cry2, Per1, Per2, and Per3 was also substantially altered. The oscillatory expression of Bmal1 was maintained in rats imperiled to SE, but with lower amplitude (A = 0.2) and an advanced acrophase in the epileptic phase. The diurnal rhythm of Cry1 and Cry2 was absent in the early post-SE but was recovered in the epileptic phase. Per1 and Per2 rhythmic expression were disrupted in post-SE groups while Per3 presented an arrhythmic profile in the epileptic phase, only. The expression of Clock did not display rhythmic pattern in any condition. These oscillating patterns of core clock genes may contribute to hippocampal 24 h cycling and, consequently to seizure periodicity. Furthermore, by using a pool of samples collected at 6 different Zeitgeber Times (ZT), we found that all clock transcripts were significantly dysregulated after SE induction, except Per3 and Per2. Collectively, altered SLA rhythm in early post-SE and epileptic phases implies a possible role for seizure as a nonphotic cue, which is likely linked to activation of hippocampal–accumbens pathway. On the other hand, altered temporal expression of the clock genes after SE suggests their involvement in the MTLE.
Usual normalization strategies for gene expression studies impair the detection and analysis of circadian patterns Diego de Siqueira Figueredo, Mayara Rodrigues Barbosa, Daniel Gomes Coimbra, José Luiz Araújo dos Santos, Ellyda Fernanda Lopes Costa, et al. Chronobiology International, 2018 Recent studies have shown that transcriptomes from different tissues present circadian oscillations. Therefore, the endogenous variation of total RNA should be considered as a potential bias in circadian studies of gene expression. However, normalization strategies generally include the equalization of total RNA concentration between samples prior to cDNA synthesis. Moreover, endogenous housekeeping genes (HKGs) frequently used for data normalization may exhibit circadian variation and distort experimental results if not detected or considered. In this study, we controlled experimental conditions from the amount of initial brain tissue samples through extraction steps, cDNA synthesis, and quantitative real time PCR (qPCR) to demonstrate a circadian oscillation of total RNA concentration. We also identified that the normalization of the RNA’s yield affected the rhythmic profiles of different genes, including Per1-2 and Bmal1. Five widely used HKGs (Actb, Eif2a, Gapdh, Hprt1, and B2m) also presented rhythmic variations not detected by geNorm algorithm. In addition, the analysis of exogenous microRNAs (Cel-miR-54 and Cel-miR-39) spiked during RNA extraction suggests that the yield was affected by total RNA concentration, which may impact circadian studies of small RNAs. The results indicate that the approach of tissue normalization without total RNA equalization prior to cDNA synthesis can avoid bias from endogenous broad variations in transcript levels. Also, the circadian analysis of 2−Cycle threshold (Ct) data, without HKGs, may be an alternative for chronobiological studies under controlled experimental conditions.
Electrophysiological evidence that the retrosplenial cortex displays a strong and specific activation phased with hippocampal theta during paradoxical (REM) sleep Bruna Del Vechio Koike, Kelly Soares Farias, Francesca Billwiller, Daniel Almeida-Filho, Paul-Antoine Libourel, et al. Journal of Neuroscience, 2017 It is widely accepted that cortical neurons are similarly more activated during waking and paradoxical sleep (PS; aka REM) than during slow-wave sleep (SWS). However, we recently reported using Fos labeling that only a few limbic cortical structures including the retrosplenial cortex (RSC) and anterior cingulate cortex (ACA) contain a large number of neurons activated during PS hypersomnia. Our aim in the present study was to record local field potentials and unit activity from these two structures across all vigilance states in freely moving male rats to determine whether the RSC and the ACA are electrophysiologically specifically active during basal PS episodes. We found that theta power was significantly higher during PS than during active waking (aWK) similarly in the RSC and hippocampus (HPC) but not in ACA. Phase–amplitude coupling between HPC theta and gamma oscillations strongly and specifically increased in RSC during PS compared with aWK. It did not occur in ACA. Further, 68% and 43% of the units recorded in the RSC and ACA were significantly more active during PS than during aWK and SWS, respectively. In addition, neuronal discharge of RSC but not of ACA neurons increased just after the peak of hippocampal theta wave. Our results show for the first time that RSC neurons display enhanced spiking in synchrony with theta specifically during PS. We propose that activation of RSC neurons specifically during PS may play a role in the offline consolidation of spatial memories, and in the generation of vivid perceptual scenery during dreaming. SIGNIFICANCE STATEMENT Fifty years ago, Michel Jouvet used the term paradoxical to define REM sleep because of the simultaneous occurrence of a cortical activation similar to waking accompanied by muscle atonia. However, we recently demonstrated using functional neuroanatomy that only a few limbic structures including the retrosplenial cortex (RSC) and anterior cingulate cortex (ACA) are activated during PS. In the present study, we show for the first time that the RSC and ACA contain neurons firing more during PS than in any other state. Further, RSC neurons are firing in phase with the hippocampal theta rhythm. These data indicate that the RSC is very active during PS and could play a key role in memory consolidation taking place during this state.
Screening for polymorphisms in the HIOMT gene and associations with circadian phenotypes Sleep Science, 2013
Screening for polymorphisms in the AANAT gene and their association with extreme diurnal preference Sleep Science, 2013
Dissociation of circadian rhythms in adolescent rats affects object recognition and spatial recognition memories KC Pugliane, R Castelo-Branco, KKBC Araújo, JC Pereira, ... Physiology & Behavior 292, 114824 , 2025 2025 Citations: 2
Clock-related genes disruption and melatonin administration are associated with melanoma and oral cancer, but not with skin carcinoma AKF Duarte, ESB Gomes, RG Rocha, ALS Guimarães, GC Omena, ... 2024
Pre-metastatic niche in oral squamous cell carcinoma: Insights from a transcriptomic meta-analysis FDA KELLY, FH DE ALMEIDA, DEONG CASTRO, LRR MARCEL, ... GENE 3 (4), 2971 , 2024 2024
Efficiency of daily kombucha consumption in reducing glycemic levels and hypercholesterolemia AB Barroso Filho, LAO Barros, KRC Ribeiro, CA Silva, BDV Koike Brazilian J Heal Rev 7 (2), e68785 , 2024 2024 Citations: 3
Shift work and metabolic syndrome updates: a systematic review MAS Schettini, RF do Nascimento Passos, BDV Koike Sleep Science 16 (02), 237-247 , 2023 2023 Citations: 47
Clinical evolution of COVID-19 patients with hypertension and/or diabetes in the intensive care units: a systematic review S Falcão, QS de Sena, RRC da Silva, DM Ribeiro, CR Naue, BDV Koike Revista de Ensino, Ciência e Inovação em Saúde 4 (1), 27-38 , 2023 2023
Imunopatogênese no desenvolvimento da COVID-19 CA Silva, AFS Freitas, BB Martins, E de Jesus Sá, GHD da Silva, ... REVISTA SAÚDE & CIÊNCIA 10 (1), 85-102 , 2021 2021 Citations: 6
Hippocampus-retrosplenial cortex interaction is increased during phasic REM and contributes to memory consolidation DG de Almeida-Filho, BDV Koike, F Billwiller, KS Farias, IRP de Sales, ... Scientific reports 11 (1), 13078 , 2021 2021 Citations: 60
Correlation between circadian rhythm related genes, type 2 diabetes, and cancer: Insights from metanalysis of transcriptomics data TKB Vieira, MJ da Rocha Leão, LX Pereira, LCA da Silva, BBP da Paz, ... Molecular and cellular endocrinology 526, 111214 , 2021 2021 Citations: 19
I EVENTO DA SEMANA DO CEREBRO DA UNIVERSIDADE FEDERAL DO VALE SÃO FRANCISCO, PETROLINA/PE: O CONHECIMENTO DA POPULAÇÃO SOBRE OS SINAIS E SINTOMAS DO ACIDENTE VASCULAR ENCEFÁLICO EAC Marcos, BDV Koike Interfaces-Revista de Extensão da UFMG, 213-226 , 2020 2020
Is there a relationship between hippocampus-dependent memory and 5-ht2a receptors? Insights from a systematic review/Há uma relação entre a memória hipocampo-dependente e … RD da Silva Miguel, JEB Barros, GFN Junior, IC da Silva Costa, JE Xavier, ... Brazilian Journal of Health Review 3 (2), 2355-2373 , 2020 2020
IS THERE A RELATIONSHIP BETWEEN HIPPOCAMPUS-DEPENDENT MEMORY AND 5-HT2A RECEPTORS? INSIGHTS FROM A SYSTEMATIC REVIEW MRD SILVA, JEB BARROS, JGF NOBRE, CIC SILVA, JE XAVIER, ... BRAZILIAN JOURNAL OF HEALTH REVIEW Учредители: Brazilian Journals … , 2020 2020
Is there a relationship between hippocampus-dependent memory and 5-ht2a receptors? Insights from a systematic review/Há uma relação entre a memória hipocampo-dependente e … RDS Miguel, JEB Barros, GFN Junior, ICS Costa, JE Xavier, LS Oliveira, ... 2020
Análise da qualidade de sono e da preferência diurna dos trabalhadores em turnos da área da saúde PVF Medrado, AM dos Santos, BDV Koike Revista de Ensino, Ciência e Inovação em Saúde 1 (1), 44-54 , 2020 2020
A Distribuição Geográfica dos casos da COVID-19 no Município de Petrolina, Pernambuco, Brasil EAC Marcos, MEC dos Santos, JS de Andrade, BDV Koike Revista de Ensino, Ciência e Inovação em Saúde 1 (1), 83-90 , 2020 2020 Citations: 2
Schwann cell reprogramming and lung cancer progression: a meta-analysis of transcriptome data VM Silva, JA Gomes, LPG Tenório, GC de Omena Neta, ... Oncotarget 10 (68), 7288 , 2019 2019 Citations: 21
Correlation between renin-angiotensin system (RAS) related genes, type 2 diabetes, and cancer: Insights from metanalysis of transcriptomics data LX Pereira, LCA da Silva, A de Oliveira Feitosa, RJS Ferreira, AKF Duarte, ... Molecular and cellular endocrinology 493, 110455 , 2019 2019 Citations: 9
Forced internal desynchrony induces cardiometabolic alterations in adult rats IGB de Oliveira, MDF Junior, PR Lopes, DBT Campos, ML Ferreira-Neto, ... Journal of Endocrinology 242 (2), 25-36 , 2019 2019 Citations: 15
Melatonin receptor 1B− 1193T> C polymorphism is associated with diurnal preference and sleep habits ACP e Silva, MJ Dos Santos, BDV Koike, MSA Moreira, DLG Gitai, ... Sleep Medicine 53, 106-114 , 2019 2019 Citations: 15
Rhythms of Core Clock Genes and Spontaneous Locomotor Activity in Post- Status Epilepticus Model of Mesial Temporal Lobe Epilepsy HC Matos, BDV Koike, WS Pereira, TG De Andrade, OW Castro, ... Frontiers in neurology 9, 632 , 2018 2018 Citations: 51
MOST CITED SCHOLAR PUBLICATIONS
Clock polymorphisms and circadian rhythms phenotypes in a sample of the Brazilian population M Pedrazzoli, FM Louzada, DS Pereira, AA Benedito‐Silva, AR Lopez, ... Chronobiology international 24 (1), 1-8 , 2007 2007 Citations: 138
Electrophysiological evidence that the retrosplenial cortex displays a strong and specific activation phased with hippocampal theta during paradoxical (REM) sleep BDV Koike, KS Farias, F Billwiller, D Almeida-Filho, PA Libourel, ... Journal of Neuroscience 37 (33), 8003-8013 , 2017 2017 Citations: 87
Hippocampus-retrosplenial cortex interaction is increased during phasic REM and contributes to memory consolidation DG de Almeida-Filho, BDV Koike, F Billwiller, KS Farias, IRP de Sales, ... Scientific reports 11 (1), 13078 , 2021 2021 Citations: 60
Rhythms of Core Clock Genes and Spontaneous Locomotor Activity in Post- Status Epilepticus Model of Mesial Temporal Lobe Epilepsy HC Matos, BDV Koike, WS Pereira, TG De Andrade, OW Castro, ... Frontiers in neurology 9, 632 , 2018 2018 Citations: 51
Shift work and metabolic syndrome updates: a systematic review MAS Schettini, RF do Nascimento Passos, BDV Koike Sleep Science 16 (02), 237-247 , 2023 2023 Citations: 47
Do Caucasian and Asian clocks tick differently? AA Barbosa, M Pedrazzoli, BDV Koike, S Tufik Brazilian Journal of Medical and Biological Research 43 (1), 96-99 , 2010 2010 Citations: 38
Interactions of polymorphisms in different clock genes associated with circadian phenotypes in humans M Pedrazzoli, R Secolin, LOB Esteves, DS Pereira, BDV Koike, ... Genetics and Molecular Biology 33, 627-632 , 2010 2010 Citations: 30
Schwann cell reprogramming and lung cancer progression: a meta-analysis of transcriptome data VM Silva, JA Gomes, LPG Tenório, GC de Omena Neta, ... Oncotarget 10 (68), 7288 , 2019 2019 Citations: 21
Correlation between circadian rhythm related genes, type 2 diabetes, and cancer: Insights from metanalysis of transcriptomics data TKB Vieira, MJ da Rocha Leão, LX Pereira, LCA da Silva, BBP da Paz, ... Molecular and cellular endocrinology 526, 111214 , 2021 2021 Citations: 19
The G619A Aa-nat Gene Polymorphism does not Contribute to Sleep Time Variation in the Brazilian Population DS Pereira, M Pedrazzoli, BDV Koike, FM Louzada, AA Benedito-Silva, ... Behavior genetics 37 (4), 637-638 , 2007 2007 Citations: 18
Forced internal desynchrony induces cardiometabolic alterations in adult rats IGB de Oliveira, MDF Junior, PR Lopes, DBT Campos, ML Ferreira-Neto, ... Journal of Endocrinology 242 (2), 25-36 , 2019 2019 Citations: 15
Melatonin receptor 1B− 1193T> C polymorphism is associated with diurnal preference and sleep habits ACP e Silva, MJ Dos Santos, BDV Koike, MSA Moreira, DLG Gitai, ... Sleep Medicine 53, 106-114 , 2019 2019 Citations: 15
Correlation between renin-angiotensin system (RAS) related genes, type 2 diabetes, and cancer: Insights from metanalysis of transcriptomics data LX Pereira, LCA da Silva, A de Oliveira Feitosa, RJS Ferreira, AKF Duarte, ... Molecular and cellular endocrinology 493, 110455 , 2019 2019 Citations: 9
Imunopatogênese no desenvolvimento da COVID-19 CA Silva, AFS Freitas, BB Martins, E de Jesus Sá, GHD da Silva, ... REVISTA SAÚDE & CIÊNCIA 10 (1), 85-102 , 2021 2021 Citations: 6
Usual normalization strategies for gene expression studies impair the detection and analysis of circadian patterns DS Figueredo, MR Barbosa, DG Coimbra, JLA Dos Santos, EFL Costa, ... Chronobiology International 35 (3), 378-391 , 2018 2018 Citations: 5
Recording day and night: advice for new investigators in the sleep and memory field AC Souza, BDV Koike, R Pedrosa, ABL Tort, CM Queiroz, S Ribeiro Handbook of behavioral neuroscience 28, 43-62 , 2018 2018 Citations: 5
Screening for polymorphisms in the AANAT gene and their association with extreme diurnal preference BDV Koike 2013 Citations: 5
Rhythms of core clock genes and spontaneous locomotor activity in Post-Status Epilepticus Model of Mesial temporal lobe Epilepsy. Front Neurol. 2018; 9: 632 HC Matos, BDV Koike, WDS Pereira, TG De Andrade, OW Castro, ... 2018 Citations: 4
Efficiency of daily kombucha consumption in reducing glycemic levels and hypercholesterolemia AB Barroso Filho, LAO Barros, KRC Ribeiro, CA Silva, BDV Koike Brazilian J Heal Rev 7 (2), e68785 , 2024 2024 Citations: 3
Avaliação comportamental e neuroquímica de ratos em dessincronização forçada: possíveis implicações para um modelo animal de oscilações no humor BDV Koike Universidade Federal do Rio Grande do Norte , 2013 2013 Citations: 3
