Bhushan Kulkarni

@klepkbsrc.org

Scientist Grade II, Dr. Prabhakar Kore Basic Science Research Centre
KLE University

Bhushan Kulkarni


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https://researchid.co/bhushan.b.kulkarni
10

Scopus Publications

275

Scholar Citations

7

Scholar h-index

6

Scholar i10-index

Scopus Publications

  • Risk factors in glaucoma: Insights from a prospective case–control study, Belagavi, India
    Asawari Kabure, Bhushan Kulkarni, Shilpa Kodkany, Murigendra Hiremath
    South Sudan Medical Journal, 2026
    Introduction: Glaucoma is an optic neuropathy and degeneration of retinal ganglion cells, causing irreversible visual damage and blindness. While raised intraocular pressure (IOP) is the most important established risk factor, the exact pathogenesis and other risk factors for glaucoma are not fully understood, particularly in developing countries. Early identification and management of risk factors are important for reducing incidence and burden. This study assessed the risk factors for glaucoma at Dr. Kodkany’s Eye Center in Belagavi, India.Method: This case-control study examined 24 cases with confirmed diagnosis of glaucoma and 20 age- and sex-matched controls. A pre-designed questionnaire was used to collect sociodemographic data along with detailed eye examination findings. All glaucoma participants underwent thorough ophthalmic examinations, including visual acuity, intraocular pressure (IOP), central corneal thickness, anterior segment abnormalities, anterior chamber angle, fundus photography, and visual field testing.Results: Of the 24 cases, 14 (58.3%) were diagnosed with open-angle glaucoma, with bilateral involvement noted in 13(54.2%) cases. IOP was considerably higher in glaucomatous patients compared to non-glaucomatous (right eye: 27.86±8.97 vs 15.85±2.84 mmHg [Mean difference: 12.01 (95% CI: 8.24–15.78]; left eye: 28.27±8.75 vs.16.05 ± 3.02 mmHg; [Mean difference: 12.22 (95% CI: 8.49–15.95)] p<0.001 for both). None of the studied factors — age (OR=0.990, 95% CI: 0.937-1.045, p=0.705), female gender (OR=1.667, 95% CI: 0.500-5.559, p=0.416), or hypertension (OR=2.538, 95% CI: 0.697-9.240, p=0.160)—predicted glaucoma risk.Conclusion: This study confirms that elevated IOP is a key characteristic of glaucoma. However, age, female gender, and hypertension were not recognised as important risk factors for glaucoma. This suggests that other risk factors might have contributed to the risk of glaucoma. Screening beyond traditional risk factors helps in identifying at-risk patients.
  • Genetic Silhouettes in Congenital Hearing Loss: Tracing GJB2 Inheritance Through the Lens of Consanguineous Marriages
    Sangamitra Vijayakumar, Priti S. Hajare, Bhushan B. Kulkarni, Ramesh S. Paranjape, Mehreen Salim Belawadi
    Indian Journal of Otolaryngology and Head and Neck Surgery, 2025
  • In vitro antioxidant and anticancer potential of Annona squamosa L. Extracts against breast cancer
    Nehal Rami, Bhushan Kulkarni, Sandesh Chibber, Devendrasinh Jhala, Nilam Parmar, Krupali Trivedi
    International Journal of Experimental Research and Review, 2023
    Various ailments have long been treated with plant-based remedies. This research aims to determine which compounds are present in Annona squamosa L. leaves and seeds extracts, as well as their antioxidant and anticancer potential. Annona squamosa L. hexane, methanol, and aqueous extracts were analysed for their phytochemical content, and results showed the existence of many useful compounds. The DPPH assay was used to measure the antioxidant activity. In particular, the low IC50 values revealed that the plant extracts under study possessed remarkable antioxidant activities. Antioxidant phytochemicals such alkaloid, flavonoid, phenol, oil, phytosterol, coumarin, and saponins may be responsible for these benefits. This research shows that extracts of Annona squamosa L. have a high antioxidant activity and promise as a dietary supplement and possible medicinal agent. MTT assay was used to test for anticancer characteristics. Cell viability was assessed for each extract using a negative control and a positive control (Cisplatin). Both the Annona squamosa L. (seeds) methanolic extract and the Annona squamosa L. (leaves) water extract showed substantial efficacy in this investigation against the MCF-7 breast cancer cell line. It follows that extracts of Annona squamosa L. (leaves) and (seeds) could be used to create a natural medicine for breast cancer treatment.
  • Method Development and Qualification of pH‐Based CEX UPLC Method for Monoclonal Antibodies
    Mithun Bhatt, Anshu Alok, Bhushan B. Kulkarni
    Biotech, 2022
    Post-translational modifications (“PTMs”) in monoclonal antibodies (mAbs) contribute to charge variant distribution, which will affect biological efficacy and safety. For the characterization of mAbs, charge variants are used as a critical quality attributes for product quality, stability consistency and effectiveness. Charge variants in mAbs are characterized by a time-consuming and a multistep process starting from cation/anion exchange chromatography, acidic/basic fractions collection and subsequent reverse phase (RP) liquid chromatography, coupled with mass spectrometry (MS) analysis. Hence, an alternative characterization approach that would be highly selective for ion exchange chromatography-based charge variant analysis, which is compatible with on-line MS detection, is needed in the biopharma industry. Against this backdrop, multiple studies are being conducted to develop a simple straight on-line charge variant analysis method. In this regard, we apply the current study, which aims to develop a charge variant analytical method, based on volatile buffers with low ionic strength that can be used for on-line MS detection of charge variants of mAbs. This would enable the detection on “PTMs” using low ionic strength mobile phase compatible with MS. Hence, fruitful data can be obtained with a single chromatography run without any test sample preparation, eliminating the need for multiple steps of analysis, time-consuming process and multiple sample preparation steps. Thus, Charge Variant Analysis-MS technique will allow the characterization of charge-related PTMs on the intact protein stage. In this regard, this study is about development of a method having combination of chromatography and volatile mobile phase for mass spectrometry detection of mAbs being analyzed in native form. The method is qualified considering pharmacopeia guidelines because the ultimate aim is to transfer this method for Quality Control (QC) release testing of a monoclonal antibody, which is critical for batch release and the regulatory point of view. Acidic and basic variants have been separated with high resolution peak profile. Furthermore, there was no matrix interference and good separation selectivity in terms of specificity was obtained using this method. The experimental data suggested for the linearity of the method are 2.4 mg/mL to 3.6 mg/mL with % RSD below 2.0%. Additionally, Limit of Quantitation is found to be 0.15 mg/mL, which is 5% of loading amount. Consistently, the data show that the method is precise under the same operating conditions with a short time interval. Overall a simple, accurate, robust and precise pH gradient cation exchange chromatography method was developed and qualified for the characterization of a therapeutic native mAb. Additionally, this method can be used to claim a biosimilar product profile of an in-house product compare to an innovator.
  • Antibiofilm efficacy of root-end filling materials against Enterococcus faecalis - An in vitro study
    Geeta Hiremath, Madhu Singh, B. Kulkarni, Rajeev Potdar, B. Naik
    Endodontology, 2020
    Aim of the Study: This study aims to evaluate the antibiofilm activity of root end materials against Enterococcus faecalis. Materials and Methods: Mineral trioxide aggregate (MTA), MTA plus and Biodentine were conjugated with chitosan gel and tested against the 3-day biofilm of E. faecalis. The incubated plates were stained using crystal violet stain and the optical density of adherent stained biofilm was read at 590 nm using ELISA auto reader. Results: There was a mean clinical reduction in the biofilms of the conjugates as compared to their individual counter parts. There was a statistically significant difference seen between the groups (MTA Plus – Chitosan Conjugate) and (MTA – Chitosan Conjugate) with P = 0.0495. Conclusion: The conjugates did perform better in inhibiting the biofilm activity of E. faecalis. Although all conjugates formed were not statistically significant.
  • P53 polymorphism and association of human papillomavirus in oral submucous fibrosis and oral squamous cell carcinoma: A case-control study
    K. Hallikeri, K. Burde, Venktesh Anehosur, B. Kulkarni, S. V. Hiremath
    Journal of Oral and Maxillofacial Pathology, 2019
    Introduction: The tumor-suppressor p53 protein is inactivated by the human papillomavirus (HPV) E6 oncoprotein, causing polymorphism of the p53 at codon 72 of exon either proline (Pro) or arginine (Arg). Specific allele predisposition has been reported in the literature. The association between the p53 allele and HPV types has been reported. We analyzed the association between p53 polymorphism at codon 72 and HPV 16 and 18 genotypes in control, oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC). Materials and Methods: Of the total 90 cases, biopsy tissues of all groups (30 cases of OSF, OSCC and control each) were collected to extract DNA. Polymerase chain reaction was used to detect HPV 16 and 18 and alleles of codon 72 in p53 were evaluated in all the samples. Results: In control, OSF and OSCC samples showed the presence HPV 63.3%, 33.3% and 60%, respectively. In OSF, HPV 16 and 18 was detected in four and four cases, respectively, whereas in OSCC, HPV 16 and 18 was detected in ten and nine cases, respectively. In all three groups, predominantly, Arg/Arg protein was present followed by Pro/Pro and Arg/Pro. Among the control, Arg/Arg type protein was frequently seen followed by Arg/Pro, Pro/Pro in the presence of HPV. OSF and OSCC were associated homologous genes in the presence of HPV. Conclusion: The definite association between p53 codon 72, polymorphism and HPV 16 and 18 was seen in OSCC with low frequency in OSF. Frequency of homozygous genotype is at high risk in the presence of HPV 16 and 18 in developing OSCC.
  • Genomic DNA of MCF-7 breast cancer cells not an ideal choice as positive control for PCR amplification based detection of Mouse Mammary Tumor Virus-Like Sequences
    Bhushan B. Kulkarni, Shivaprakash V. Hiremath, Suyamindra S. Kulkarni, Umesh R. Hallikeri, Basavaraj R. Patil, Pramod B. Gai
    Journal of Virological Methods, 2013
  • Decade of breast cancer-trends in patients profiles attending tertiary cancer care center in South India
    B.B. Kulkarni, S.V. Hiremath, S.S. Kulkarni, U.R. Hallikeri, B.R. Patil, P.B. Gai
    Asian Journal of Epidemiology, 2012
  • Molecular basis of β-thalassemia in Karnataka, India
    Gururaj D. Kulkarni, Suyamindra S. Kulkarni, Gurushantappa S. Kadakol, Bhushan B. Kulkarni, Prakashgouda H. Kyamangoudar, Bhaskar V.K.S. Lakkakula, Kumarasamy Thangaraj, Tipperudra A. Shepur, Muralidhar L. Kulkarni, Pramod B. Gai
    Genetic Testing and Molecular Biomarkers, 2012
    In β-thalassemia, point mutations in the β-globin gene are largely responsible for either decreased or no β-globin synthesis. The β-globin gene has three exons and two introns. The molecular characterization of β-thalassemia is absolutely necessary for carrier screening, for genetic counseling, and to offer prenatal diagnosis. The objective of the present study was to identify the rare mutations in β-globin gene of β-thalassemia patients. We have sequenced the entire β-globin gene in 36 clinically identified thalassemia patients from the Karnataka region using polymerase chain reaction and sequencing. Our analysis revealed 11 β-thalassemia variants. The most common being IVSII-16 G>C, IVSI-5G>C, IVSII-74 T>G, codon 3 (T>C), and Poly A site (T>C). In addition, we have also documented a novel deletion at codon 6 (-CT) (HBB:c.16delCT). These data are useful in future molecular screening of the population for implementing a thalassemia prevention and control program. Further it is found that family studies and comprehensive hematological analyses would provide useful insights for accurate molecular diagnosis of thalassemia phenotype and offers an interesting subject for further investigations in the Indian populations.
  • Prevalence and distribution of high risk human papillomavirus (HPV) types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in the general population in Karnataka, India
    Asian Pacific Journal of Cancer Prevention, 2011

RECENT SCHOLAR PUBLICATIONS

  • Association of Trabecular Meshwork-Related Gene Variants with Glaucoma: Insights from the Belagavi Region of Karnataka
    A Kabure, B Kulkarni, S Kodkany, N Harale, M Hiremath
    2026
  • Genetic Silhouettes in Congenital Hearing Loss: Tracing GJB2 Inheritance Through the Lens of Consanguineous Marriages
    S Vijayakumar, PS Hajare, BB Kulkarni, RS Paranjape, MS Belawadi
    Indian Journal of Otolaryngology and Head & Neck Surgery 77 (12), 5668-5674 , 2025
    2025
  • Exploring Blood-Based Methylation of BRCA Genes in Breast Cancer: Advances, Applications, and Challenges
    P Sahana Ulagaddi, B Kulkarni Bhushan
    Research Journal of Pharmacy and Medical Sciences (IRJPMS) 8 (4), 53-55 , 2025
    2025
  • Phytochemical Analysis And In Silico Pharmacological Profiling Of Methanolic Extract From Annona Squamosa L. Seeds For Breast Cancer
    N RAMI, B Kulkarni, S Chibber
    Educational Administration: Theory and Practice 30 (5), 8871-8883 , 2024
    2024
    Citations: 1
  • Investigation of Anti-obesity Potential of Simarouba glauca Leaves Extract Using in-vitro Method
    SCG Sweta Gupta, Sridevi I Puranik, Rubeen Nadaf, M A Mujeeb, Bhushan ...
    Karnatak University Journal of Science 54 (4), 68-72 , 2023
    2023
  • In vitro antioxidant and anticancer potential of Annona squamosa L. Extracts against breast cancer
    N Rami, B Kulkarni, S Chibber, D Jhala, N Parmar, K Trivedi
    Int. J. Exp. Res. Rev 30, 264-275 , 2023
    2023
    Citations: 16
  • Method Development and Qualification of pH-Based CEX UPLC Method for Monoclonal Antibodies
    M Bhatt, A Alok, BB Kulkarni
    BioTech 11 (2), 19 , 2022
    2022
    Citations: 5
  • A STUDY ON ASSOCIATION OF TETRANUCLEOTIDE DUPLICATION (-GGCC-) OF TPO GENE WITH HYPOTHYROIDISM
    AR Kabure, BB Kulkarni
    Journal of Advanced Scientific Research 2021 (ISTCMS), 08-12 , 2021
    2021
  • Antibiofilm efficacy of root-end filling materials against Enterococcus faecalis-An in vitro study
    G Hiremath, M Singh, B Kulkarni, R Potdar, B Naik
    Endodontology 32 (2), 86-90 , 2020
    2020
    Citations: 6
  • p53 polymorphism and association of human papillomavirus in oral submucous fibrosis and oral squamous cell carcinoma: A case–control study
    K Hallikeri, K Burde, V Anehosur, BB Kulkarni, SV Hiremath
    Journal of Oral and Maxillofacial Pathology 23 (1), 97-103 , 2019
    2019
    Citations: 20
  • Hepatoprotective activity of methanolic shoot extract of Bambusa bambos against carbon tetrachloride induce acute liver toxicity in Wistar rats
    AASS Patil, HJ Hoskeri, P Rajeev, B Kulkarni, GR Kamble, GB Hiremath, ...
    Journal of Applied Biology & Biotechnology Vol 6 (04), 37-40 , 2018
    2018
    Citations: 7
  • Association of Interleukin-1β+ 3954 (Rs1143634) Gene Polymorphism in Periodontal Healthy and Chronic Periodontitis Patients
    S Setty, DPR Bhat, B Kulkarni, DSV Hiremath, S Thakur
    IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 17 (8), 58-63 , 2018
    2018
    Citations: 2
  • Etiopathogenesis of Oral Lichen Planus and Epstein-Barr Virus in India
    KR Nandimath, BB Kulkarni, K Burde, SV Hiremath
    2018
    Citations: 1
  • Studies on Isolation and Quantification of Lycopene from Tomato and Papaya and its Antioxidant and Antifungal Properties
    PR Desai, PD Holihosur, SV Marathe, BB Kulkarni, V UK, RK Geetanjali, ...
    2018
    Citations: 17
  • Study on Decolorization of Bromophenol Blue and Crystal Violet by Isolates Pseudomonas Sp. and Salmonella Sp.
    Potadar R. R., Shetti A. A., Hiremath S. V., Halarnkar D. J., Panicker S. R ...
    International Journal of Recent Scientific Research 8 (6), 17628-17633 , 2017
    2017
    Citations: 3
  • Effects of salivary e-cadherin (cdh1-160) gene promoter polymorphism on the risk of development of oral cancer in indian origin
    SV Namratha Kaviraj Karkera, Kruthika S Guttal, Praveen Kumar Shetty, Anil ...
    International Journal of Current Research 9 (04), 49449-49454 , 2017
    2017
    Citations: 2
  • Antibacterial Potentials of Red Pigment Extracted From Soil Isolate Serratia Sp.
    2. Shetti A. A., Kreeti Patil, Potadar R. R., Kulkarni B. B., G B. Hiremath ...
    IOSR Journal of Biotechnology and Biochemistry (IOSR - JBB) 3 (4), 49-52 , 2017
    2017
  • Isolation and characterization of Biosurfactant Produced by Soil Isolates Pseudomonas species and Rhodococcus sp
    Geetanjali R K, Prajnya A M, Shruti A J, Sunita N, Bhushan B K Potadar R. R ...
    IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) 12 (5), 17-20 , 2017
    2017
    Citations: 1
  • Vascular endothelial growth factor (VEGF) gene polymorphism in oral submucous fibrosis subjects-A preliminary study
    DV Rai, KS Guttal, BB Kulkarni, S Hiremath, KN Burde
    Asian Journal of Medical Sciences 7 (5), 10-16 , 2016
    2016
    Citations: 4
  • Snap shot of epidemiological pattern of cervical cancer patients reporting to a tertiary cancer care center in North Karnataka, India.
    S Shanthala, BS Agadi, BB Kulkarni, BR Patil, UR Hallikeri, ...
    International Journal of Advanced Biological Research 4 (2), 178-183 , 2014
    2014
    Citations: 5

MOST CITED SCHOLAR PUBLICATIONS

  • Prevalence and distribution of high risk human papillomavirus (HPV) types 16 and 18 in carcinoma of cervix, saliva of patients with oral squamous cell carcinoma and in the …
    SS Kulkarni, SS Kulkarni, PP Vastrad, BB Kulkarni, AR Markande, ...
    Asian Pac J Cancer Prev 12 (3), 645-648 , 2011
    2011
    Citations: 112
  • Decade of Breast Cancer-Trends in Patients Profiles Attending Tertiary Cancer Care Center in South India.
    BB Kulkarni, SV Hiremath, SS Kulkarni, UR Hallikeri, BR Patil, PB Gai
    Asian Journal of Epidemiology 5 (4) , 2012
    2012
    Citations: 26
  • Molecular basis of β-thalassemia in Karnataka, India
    GD Kulkarni, SS Kulkarni, GS Kadakol, BB Kulkarni, PH Kyamangoudar, ...
    Genetic testing and molecular biomarkers 16 (2), 138-141 , 2012
    2012
    Citations: 24
  • p53 polymorphism and association of human papillomavirus in oral submucous fibrosis and oral squamous cell carcinoma: A case–control study
    K Hallikeri, K Burde, V Anehosur, BB Kulkarni, SV Hiremath
    Journal of Oral and Maxillofacial Pathology 23 (1), 97-103 , 2019
    2019
    Citations: 20
  • Studies on Isolation and Quantification of Lycopene from Tomato and Papaya and its Antioxidant and Antifungal Properties
    PR Desai, PD Holihosur, SV Marathe, BB Kulkarni, V UK, RK Geetanjali, ...
    2018
    Citations: 17
  • In vitro antioxidant and anticancer potential of Annona squamosa L. Extracts against breast cancer
    N Rami, B Kulkarni, S Chibber, D Jhala, N Parmar, K Trivedi
    Int. J. Exp. Res. Rev 30, 264-275 , 2023
    2023
    Citations: 16
  • Mutation analysis of the LDL receptor gene in Indian families with familial hypercholesterolemia
    SS Kulkarni, S Basavraj, GS Kadakol, A Markande, J Sindhoor, ...
    Asian Journal of Medical Sciences 2 (2), 82-86 , 2011
    2011
    Citations: 8
  • Hepatoprotective activity of methanolic shoot extract of Bambusa bambos against carbon tetrachloride induce acute liver toxicity in Wistar rats
    AASS Patil, HJ Hoskeri, P Rajeev, B Kulkarni, GR Kamble, GB Hiremath, ...
    Journal of Applied Biology & Biotechnology Vol 6 (04), 37-40 , 2018
    2018
    Citations: 7
  • Antibiofilm efficacy of root-end filling materials against Enterococcus faecalis-An in vitro study
    G Hiremath, M Singh, B Kulkarni, R Potdar, B Naik
    Endodontology 32 (2), 86-90 , 2020
    2020
    Citations: 6
  • Expression profiling of brca1 gene in familial breast cancer in India
    SS Kulkarni, BB Kulkarni, T Vandana, SS Kulkarni, GS Kadakol, H Modak, ...
    African Journal of Biotechnology 11 (37), 9123-9126 , 2012
    2012
    Citations: 6
  • Method Development and Qualification of pH-Based CEX UPLC Method for Monoclonal Antibodies
    M Bhatt, A Alok, BB Kulkarni
    BioTech 11 (2), 19 , 2022
    2022
    Citations: 5
  • Snap shot of epidemiological pattern of cervical cancer patients reporting to a tertiary cancer care center in North Karnataka, India.
    S Shanthala, BS Agadi, BB Kulkarni, BR Patil, UR Hallikeri, ...
    International Journal of Advanced Biological Research 4 (2), 178-183 , 2014
    2014
    Citations: 5
  • Parkinson’s disease in North Karnataka
    GS Kadakol, SS Kulkarni, BB Kulkarni, SS Kulkarni
    An epidemiological perspective Antrocom Online J Anthropol 8, 1973-2880 , 2012
    2012
    Citations: 5
  • Vascular endothelial growth factor (VEGF) gene polymorphism in oral submucous fibrosis subjects-A preliminary study
    DV Rai, KS Guttal, BB Kulkarni, S Hiremath, KN Burde
    Asian Journal of Medical Sciences 7 (5), 10-16 , 2016
    2016
    Citations: 4
  • Study on Decolorization of Bromophenol Blue and Crystal Violet by Isolates Pseudomonas Sp. and Salmonella Sp.
    Potadar R. R., Shetti A. A., Hiremath S. V., Halarnkar D. J., Panicker S. R ...
    International Journal of Recent Scientific Research 8 (6), 17628-17633 , 2017
    2017
    Citations: 3
  • Association of Interleukin-1β+ 3954 (Rs1143634) Gene Polymorphism in Periodontal Healthy and Chronic Periodontitis Patients
    S Setty, DPR Bhat, B Kulkarni, DSV Hiremath, S Thakur
    IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 17 (8), 58-63 , 2018
    2018
    Citations: 2
  • Effects of salivary e-cadherin (cdh1-160) gene promoter polymorphism on the risk of development of oral cancer in indian origin
    SV Namratha Kaviraj Karkera, Kruthika S Guttal, Praveen Kumar Shetty, Anil ...
    International Journal of Current Research 9 (04), 49449-49454 , 2017
    2017
    Citations: 2
  • Genomic DNA of MCF-7 breast cancer cells not an ideal choice as positive control for PCR amplification based detection of Mouse Mammary Tumor Virus-Like Sequences
    BB Kulkarni, SV Hiremath, SS Kulkarni, UR Hallikeri, BR Patil, PB Gai
    Journal of virological methods 193 (2), 304-307 , 2013
    2013
    Citations: 2
  • A comparative study of cDNA microarray analysis of familial and sporadic breast cancer in India
    SS Kulkarni, BB Kulkarni, T Vandana, SS Kulkarni, VB Shepur, ...
    J. Med. Genet 4 (1), 6-12 , 2012
    2012
    Citations: 2
  • Phytochemical Analysis And In Silico Pharmacological Profiling Of Methanolic Extract From Annona Squamosa L. Seeds For Breast Cancer
    N RAMI, B Kulkarni, S Chibber
    Educational Administration: Theory and Practice 30 (5), 8871-8883 , 2024
    2024
    Citations: 1