maria isabel mello martins

@uel.br

Associate Professor, Departamento de Clínicas Veterinárias/ Universidade Estadual de Londrina
Universidade Estadual de Londrina

maria isabel mello martins


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https://researchid.co/belmimm

RESEARCH, TEACHING, or OTHER INTERESTS

Veterinary, Small Animals, Biotechnology, General Veterinary
53

Scopus Publications

Scopus Publications

  • ProAKAP4 as a protein marker of sperm quality: Insights from frozen-thawed ejaculated and epididymal sperm
    Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Leticia Amanda dos Santos Silva, Vinicius Wagner Silva, Julia Rodrigues Greghi, et al.
    Theriogenology, 2026
  • Yolk sac development in dogs: from morphology to functional aspects
    Caio Garcia Barbosa da Silva, Julia Rodrigues Greghi, Maria Fernanda Frasson Pontes, Guilherme Schiess Cardoso, Maria Isabel Mello Martins, et al.
    Animal Reproduction, 2026
    The yolk sac (YS) is a multifunctional and essential structure for early maternal-fetal interactions, playing roles in nutrient supply, organogenesis, immune modulation, and hematopoiesis. Unlike most species, dogs maintain the YS until the end of pregnancy, yet little is known about its functional activity, particularly in later stages. Animal models are crucial for exploring maternal-fetal processes and offering insights into comparative development, including in humans. This study aimed to analyze the YS structure in canine embryos and fetuses at early (up to 20 days, n = 6), middle (21–40 days, n = 6), and late (41–60 days, n = 6) stages. Gestational age was estimated using crown-rump length (CRL) and external anatomy. Samples from the university’s Veterinary Hospital and neutering campaigns in Londrina (Paraná, Brazil) were fixed in 10% formaldehyde for morphological analysis (H&E, PAS, and Picrosirius red) or 4% paraformaldehyde for OCT4 immunostaining (CEUA/UEL protocol number 029.2022). Histologically, the YS showed a trilaminar structure with blood islands in the intermediate layer surrounded by mesenchyme. Within these islands, undifferentiated cellular niches (OCT4+) were identified. During gestation, the YS changed from a thin, smooth, pale-red membrane to a wrinkled structure with narrowed ends and a thickened central region, resembling a "whale tail." The red color, indicative of vascularization, intensified with blood island enlargement. Collagen secretion, particularly type I, progressively increased around the blood islands. Glycoprotein deposits persisted until late gestation, as shown by PAS staining. These findings highlight the structural, secretory, and reserve roles of the canine YS throughout pregnancy.
  • Influence of extenders and antioxidants on Nile tilapia (Oreochromis niloticus) sperm parameters
    Vinícius Wagner Silva, Myrian Megumy Tsunokawa Hidalgo, Renan José Casarotto Appel, Karine Nicole Siqueira, José Beirão, et al.
    Cryobiology, 2025
  • Is the proAKAP4 a suitable biomarker of X-sorted sperm quality from Nelore and Gir bulls?
    Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Luiz Guilherme Corsi Trautwein, Josiana de Fátima Schnitzer, Letícia Amanda Santos Silva, et al.
    Semina Ciencias Agrarias, 2024
    ProAKAP4 is a protein precursor of AKAP4 present in the principal piece of the flagellum, associated with sperm motility. It has been described in several species, but its concentration has never been measured in sex-sorted sperm. Thus, the study aimed to test the proAKAP4 as a suitable sperm quality biomarker for Bos indicus X-sorted sperm. To achieve the study’s goal, 14 semen straws from different bulls were evaluated, six X-sorted and eight non-sorted. Sperm kinetics and morpho-functional evaluations were carried out, in addition to the concentration of proAKAP4. The characteristics evaluated were compared by the t-test and correlated by the Pearson’s correlation. A difference was found for total motility (P = 0.014), rapid sperm (P = 0.020), and sperm area (P = 0.013) between the non-sorted and X-sorted sperm. The proAKAP4 concentration was higher in the X-sorted sperm (X-sorted sperm: 67.54 ng/106 spermatozoa; non-sorted sperm: 29.76 ng/106 spermatozoa), but it was not correlated to the kinetics or morpho-functional characteristics evaluated. However, the proAKAP4 in non-sorted sperm showed a correlation to sperm total and progressive motility, rapid sperm, and sperm membrane integrity. The study concluded on the unreliability of the proAKAP4 as a biomarker to indicate sperm quality in the X-sorted sperm.
  • Evaluation of the testicular artery Doppler velocimetry and its correlation with sperm defects in domestic cats
    Luiz Guilherme Corsi Trautwein, Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Jamile Haddad Neta, Guilherme Schiess Cardoso, et al.
    Animal Reproduction Science, 2024
  • Insights into alternative cryoprotectants to freeze sperm of domestic cats
    Maria Isabel Mello Martins, Vinícius Wagner Silva, Myrian Megumy Tsunokawa Hidalgo, Alain Fontbonne
    Reproduction in Domestic Animals, 2024
    Globalization and habitat destruction pose a significant threat to wildlife felids. Even though conservation banks for genetic materials have been created, the sperm cryopreservation with minimal cell damage is still a great challenge. Thus, this study aimed to compare the effects of two commercial extenders with different concentrations of alternative cryoprotectants on thawed sperm quality of domestic cats. Five adult cats were anaesthetized (using a combination of 40 μg/kg medetomidine associated to 5 mg/kg ketamine), and the semen was collected by electroejaculation (electrical stimulation of 2–3 V). Semen samples were evaluated for sperm characteristics (kinetics, morphology, membrane integrity and morphometry). Subsequently, they were sorted into two aliquots and centrifuged. The aliquots were added to a commercial extender containing 3% glycerol and 2% methylformamide (extender I) or 2% glycerol and 3% methylformamide (extender II), frozen, thawed (37°C/30 s) and reevaluated. Comparatively, the sperm kinetics and membrane integrity of fresh semen were higher (p < .002) than frozen samples in extender I and II. Total and progressive motility were lowest in the thawed samples. However, the subjective analysis indicated high sperm motility, since the kinetics evaluation was impaired by the low cell number in the thawed samples. There were no differences in sperm morphology between the groups. In the sperm morphometric analysis, a significant difference (p = .04) was identified in the length of the intermediate piece in extender II samples compared with fresh and extender I. Thus, it can be concluded that although the concentrations tested did not maintain the kinetic parameters and membrane integrity of spermatozoa after thawing, the extender with a lower concentration of glycerol was less toxic for maintaining the midpiece length.
  • Comparative transcriptome analysis reveals a serotype-specific immune response in Nile tilapia (Oreochromis niloticus) infected with Streptococcus agalactiae
    Renan José Casarotto Appel, Karine Nicole Siqueira, Ioannis Konstantinidis, Maria Isabel Mello Martins, Rajesh Joshi, et al.
    Frontiers in Immunology, 2024
    Streptococcus agalactiae is a major causative agent of streptococcosis in Nile tilapia (Oreochromis niloticus) and understanding its etiology is important to ensure the sustainable development of global tilapia farming. Our research group recently observed contrasting disease patterns in animals infected with two different S. agalactiae serotypes (Ib and III). To better understand the basis for these divergent responses, we analyzed the brain transcriptome of Nile tilapia following bacterial exposure. Our findings revealed significant variation in the expression of genes involved in immune (e.g., CD209 antigen, granulin, C-X-C motif chemokine 10, prostacyclin synthase, and interleukins) and neuroendocrine (e.g., mmp13a, mmp9, brain aromatase, and pmch) pathways. The serotype Ib strain seems promptly recognized by the host, triggering a potent inflammatory response, whereas the serotype III strain elicited a less immediate response, resulting in more pronounced central nervous system (CNS) symptoms and behavioral effects. To the best of our knowledge, this is the first study to show serotype-specific immune responses to S. agalactiae in Nile tilapia. These findings are important for advancing disease management and control strategies in aquaculture. Identifying different immune reactions triggered by serotypes Ib and III may assist the development of more specific approaches for preventive measures, early detection, and effective treatment against streptococcosis.
  • Disorder of Sexual Development in a French Bulldog (78, MALE XX)
    Natália Ribeiro Sambatti, Vinícius Wagner Silva, Luana Martins de Souza Amaral, Julia Rodrigues Greghi, Letícia Amanda Dos Santos Silva, et al.
    Acta Scientiae Veterinariae, 2024
    Background: Differentiation of the genital system occurs through a chronological sequence of events involving the determination of chromosomal, gonadal, and phenotypic sex. Disorders of sexual development (DSDs) include chromosomal sex abnormalities, gonadal abnormalities, and phenotypic variations. The purpose of this case report was to demonstrate the clinical signs and diagnosis by histopathology, immunohistochemistry, and karyotyping of a DSD in a French Bulldog (XX, male).Case: A 7-month-old phenotypically female French Bulldog with a history of clitoral hypertrophy and no signs of puberty is reported. The animal presented with a 3 cm micropenis protruding from the vulvar labia, causing discomfort and constant licking. Pelvic radiography confirmed the presence of a penile bone, supporting the differential for a disorder of sexual development (DSD). There were no abnormalities of the vaginal canal or urethral orifice. The animal underwent exploratory celiotomy to remove 2 uterine horns and their respective gonads, followed by clitoridectomy via episiotomy. Sex reversal was confirmed by histology, as seminiferous tubules and bilateral hypoplastic epididymides were identified on ovarian topography. Immunohistochemistry of gonadal tissues was positive with antibodies to progesterone and estrogen receptors, but negative with polyclonal androgen receptors and prostate-specific antigen. Karyotyping revealed XX sex chromosomes.Discussion: This dog was diagnosed as an XX DSD male (DSD 78, testicular XX) due to the presence of abdominal testes, uterus, predominantly female external genitalia with vulva and micropenis, and female sex chromosomes. Six cases of DSD have recently been reported in phenotypically female French Bulldogs due to the presence of clitoral hypertrophy or a micropenis with an atypical location. The increase in cases in this breed may be due to its popularity and increased consanguinity. Animals with clinical suspicion of DSD show signs during puberty and most of these animals tend to have a history of externalized structure in the vulva without signs of urethral and vaginal canal obstruction. In animals with ambiguous genitalia and DSDs, the estrous cycle may be normal, absent, or irregular. Because of the discomfort in the clitoral region, clitoridectomy is the 1 st line of treatment to prevent self-mutilation and future cases of vaginitis, and gonadectomy is also performed to prevent future uterine disorders and gonadal and mammary carcinogenesis. It is not uncommon for XX male animals to be unilateral or bilateral cryptorchids, in this case, both testes were in the abdominal cavity, and the animal had a normal uterus. Several alternative procedures can be performed for a better understanding of DSDs, namely: histopathology; immunohistochemistry or hormone dosage; karyotyping; and molecular analyses. These tests are important to determine the genetic sex of an animal and to confirm its concordance with the phenotypic sex. There is no evidence of interference of specific genes in the phenotypic expression of XX males, suggesting that the factors involved in their occurrence are still unknown. The hereditary nature of this disorder has not been ruled out and there are currently no laboratory tests available to identify carriers. It is recommended that cases of DSD be thoroughly investigated using complementary examinations to ensure accurate categorization of each disorder. Keywords: clitoral hypertrophy, ambiguous genitalia, immunohistochemistry, cytogenetics, sexual dimorphism.
  • Renal biomarkers, clinical parameters, and renal Doppler velocimetry in bitches with cystic endometrial hyperplasia–pyometra complex
    Leticia Amanda dos Santos Silva, Luiz Guilherme Corsi Trautwein, Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Julia Rodrigues Greghi, et al.
    Reproduction in Domestic Animals, 2024
    Cystic endometrial hyperplasia (CEH)–pyometra complex is the most common uterine infection in adult and elderly bitches and can cause renal dysfunction. The aim of this study was to measure and compare urinary creatinine, urea, symmetric dimethylarginine (SDMA), urinary protein–creatinine ratio (UPC), measurement of systolic blood pressure (SBP), and Doppler velocimetry of renal arteries in patients with CEH–pyometra complex before and after an average of 6 months of treatment, evaluating the possibility of the changes persisting. The evaluation was conducted at two moments: M1 (at the diagnosis of CEH–pyometra, n = 36) and M2 (after an average of six months of treatment, n = 16). For the control group, eight bitches with no changes in blood tests or history of conditions underwent Doppler ultrasound evaluation of the renal arteries. At both M1 and M2, we measured creatinine, urea, and serum SDMA, UPC, SBP, and Doppler ultrasound of the renal arteries. Patients were evaluated according to the following groups: azotemic (AZO) and non‐azotemic (NAZO), and open and closed cervix pyometra. The parameters were compared between animals present in both moments presented as M1R (bitches that were in M1 and M2) and M2. Statistical significance was considered when p < .05. The medians found for creatinine in M1 were as follows: 1.15 mg/dL, being 1.8 mg/dL for AZO (12/36) and 0.95 mg/dL for NAZO (24/36); and in M2: 0.85 mg/dL (16/16), being 1.15 mg/dL for AZO (4/16) and 0.8 mg/dL for NAZO (12/36). For urea, in M1 it was 36 mg/dL (32/36), with AZO being 103 mg/dL (11/32) and 33 mg/dL in NAZO (21/32); and in M2 32 mg/dL (16/ 16), being 29 mg/dL for AZO (4/36), and 31 mg/dL for NAZO (3/15). The median SDMA measured in M1R was 17 μg/dL (15/16), with AZO being 31 μg/dL (3/15), and NAZO being 16.5 μg/dL (12/15); and in M2, SDMA was 12 μg/dL (16/16), with AZO being 12.5 μg/dL (4/16), and NAZO being 12 μg/dL (12/16). The median UPC measured in M1 was 1.15 (10/36), with AZO being 0.25 (1/10), and NAZO being 1.38 (9/10); and in M2, it was 0.2 (13/16), being 0.1 in AZO (4/13), and 0.2 (9/16) in NAZO. For SBP, in M1, it was 118 mmHg (30/36), with AZO being 102 mmHg (10/30) and 133 mmHg in NAZO (20/30); and in M2 142.5 mmHg (12/16), being 155 mmHg for AZO (4/12), and 140 mg/dL for NAZO (8/12). When comparing animals with open and closed cervixes, a difference was found between SDMA measurements (p = .001). There was a distinction between PI and RI of the left and right kidneys consecutively (p = .007; p = .033; p = .019; p = .041). Correlations found in M1: SDMA × PI RIM DIR (r = 0.873; p = .002), SDMA × PSV RIM ESQ (r = 0.840; p = .004), SDMA × EDV RIM ESQ (r = 0.675; p = .046). With this study, we conclude a return to normality of renal biomarkers and clinical parameters after six months. Yet, there is a persistence of Doppler velocimetric measurements between the two moments. Thus, this parameter is not suitable for identifying and classifying chronic kidney injury in bitches with pyometra.
  • Emergency cesarean section in dogs: Usefulness of amniotic fluid biochemical parameters and placental morphology as indicators of neonatal viability
    Julia Rodrigues Greghi, Phelipe Oliveira Favaron, Luiz Guilherme Corsi Trautwein, Caio Garcia Barbosa da Silva, Gisele Augusta Amorim de Lemos, et al.
    Theriogenology, 2023
  • Comparison of two pharmacological semen collection times with α2-adrenergic agonist in domestic cats
    Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Leticia Amanda dos Santos Silva, Julia Rodrigues Greghi, Vinícius Wagner Silva, et al.
    Reproduction in Domestic Animals, 2023
  • Fecal microbiota profile of ovariohysterectomized cats submitted to estrogen replacement
    Jamile Haddad Neta, Luiz Guilherme Corsi Trautwein, Ana Beatriz Marques Almeida, Myrian Megumy Tsunokawa Hidalgo, Maria Isabel Mello Martins
    Brazilian Journal of Veterinary Research and Animal Science, 2023
  • The proAKAP4 concentrations in Nelore bull sperm and their relation to FTAI conception rate results
    Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Fábio Lucas Zito de Moraes, Luiz Guilherme Corsi Trautwein, Josiana de Fátima Schnitzer, et al.
    Animal Reproduction Science, 2022
  • INFLUENCE OF SPERM POST-THAW SUBPOPULATIONS OF ANGUS AND NELORE BULLS ON PREGNANCY RATES BY FIXEDTIME ARTIFICIAL INSEMINATION
    Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Fábio Lucas Zito de Moraes, Rodrigo Yudi Palhaci Marubayashi, Thales Ricardo Rigo Barreiros, et al.
    Cryo Letters, 2022
  • Assessment of thawed sperm quality from feline species: Ocelot (Leopardus pardalis) and oncilla (Leopardus gutullus)
    Jussara Maria Tebet, Fabiana Ferreira de Souza, Maria Isabel Mello Martins, Viviane Helena Chirinéa, Jaqueline Candido de Carvalho, et al.
    Theriogenology, 2022
  • Sperm subpopulations influence the pregnancy rates in cattle
    Myrian Megumy Tsunokawa Hidalgo, Ana Beatriz Marques de Almeida, Fábio Lucas Zito de Moraes, Rodrigo Yudi Palhaci Marubayashi, Fabiana Ferreira de Souza, et al.
    Reproduction in Domestic Animals, 2021
  • EVALUATION OF THE FREEZABILITY OF THE BOVINE EPIDIDYMIS TAIL SPERM WITH THE ADDITION OF ANTIOXIDANTS
    Cryo Letters, 2021
  • Electrocardiography in Bitches with Mammary Tumors
    Gabriela Bahr Arias, Mariângela Kilpp Gomes De Oliveira, Josiana de Fátima Schnitzer, Giovana Wingeter Di Santis, Maria Isabel Mello Martins, et al.
    Acta Scientiae Veterinariae, 2021
  • Sperm filtration as an alternative technique for seminal plasma separation in boars
    Carlos Augusto Alves Melanda, Ana Beatriz Marques de Almeida, Myrian Megumy Tsunokawa Hidalgo, Anne Kemmer Souza, Luiz Guilherme Corsi Trautwein, et al.
    Semina Ciencias Agrarias, 2021
  • Correlation of testicular artery Doppler velocimetry with kinetics and morphologic characteristics of epididymal sperm in dogs
    Luiz Guilherme Corsi Trautwein, Anne Kemmer Souza, Guilherme Schiess Cardoso, Karina Keller Marques da Costa Flaiban, Antonio Cezar de Oliveira Dearo, et al.
    Reproduction in Domestic Animals, 2020
  • Effect of refrigeration at -1°C on spermatozoa quality of domestic cats
    Anne K. Souza, Luiz Guilherme C. Trautwein, Cristiane S. Paranzini, Josiana F. Schnitzer, Felipe M. Perencin, et al.
    Pesquisa Veterinaria Brasileira, 2020
  • Influence of long-term thermal stress on the in vitro maturation on embryo development and Heat Shock Protein abundance in zebu cattle
    Ralf Pöhland, Mirela Brochado Souza-Cácares, Tirtha Kumar Datta, Jens Vanselow, Maria Isabel Mello Martins, et al.
    Animal Reproduction, 2020
  • Use of infrared thermography to evaluate the inflammatory reaction in cat testis after intratesticular injection of 0.9% NaCl or 20% CaCl2 with 1% lidocaine
    C.S. Paranzini, G.S. Cardoso, A.K. Souza, F.M. Perencin, C.A.A. Melanda, et al.
    Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2019
  • Oocyte quality and heat shock proteins in oocytes from bovine breeds adapted to the tropics under different conditions of environmental thermal stress
    M.B. Souza-Cácares, A.L.L. Fialho, W.A.L. Silva, C.J.T. Cardoso, R. Pöhland, et al.
    Theriogenology, 2019
  • Can testicular artery Doppler velocimetry values change according to the measured region in dogs?
    Luiz Guilherme Corsi Trautwein, Anne Kemmer Souza, Maria Isabel Mello Martins
    Reproduction in Domestic Animals, 2019
  • Protein-to-creatinine urinary in the early diagnosis of renal injury in canine pyometra
    Marcos C. Sant’Anna, Guilherme F. Martins, Karina K.M.C. Flaiban, Luiz G.C. Trautwein, Maria I.M. Martins
    Pesquisa Veterinaria Brasileira, 2019
  • Improvement on the efficiency of doses per conception by using a semen extender in timed artificial insemination
    Camila Oliveira Rosa, Denis Vinicius Bonato, Anne Kemmer Souza, Fábio Morotti, Renato Castanho Francisco, et al.
    Livestock Science, 2019
  • Sertolioma in a Canadian husky: Relationship between tumor, hormones, neurons and skin
    Jamile Haddad Neto, Letícia Amanda Dos Santos Silva, Kátia Midori Yamanishi Shimura, Rebeca Cordeiro Justino, Giovana Wingeter Dos Santos, et al.
    Acta Scientiae Veterinariae, 2019
  • Influence of cooling temperature in sperm subpopulations of domestic cats
    Anne Kemmer Souza, Luiz Guilherme Corsi Trautwein, Cristiane Sella Paranzini, Felipe Montanheiro Perencin, Guilherme Schiess Cardoso, et al.
    Animal Reproduction Science, 2018
  • Effects of chemical castration using 20% CaCl2 with 0.5% DMSO in tomcats: Evaluation of inflammatory reaction by infrared thermography and effectiveness of treatment
    Cristiane Sella Paranzini, Anne Kemmer Sousa, Guilherme Schiess Cardoso, Felipe Montanheiro Perencin, Luiz Guilherme Corsi Trautwein, et al.
    Theriogenology, 2018
  • Hypoglycemia associated with ketosis in bitches in the final phase of gestation
    Jamile Haddad Neta, Luiz Guilherme Corsi Trautwein, Maria Isabel Mello Martins
    Acta Scientiae Veterinariae, 2018
  • The importance of Gram positive bacteria as the cause of canine pyometra
    Marcos Cezar Sant' anna, Luiz Guilherme Corsi Trautwein, Lucienne Garcia Pretto Giordano, Rebeca Cordeiro Justino, Karina Keller Marques da Costa Flaiban, et al.
    Semina Ciencias Agrarias, 2017
  • The importance of Gram positive bacteria as the cause of canine pyometra
    Marcos Cezar Sant' anna, Luiz Guilherme Corsi Trautwein, Lucienne Garcia Pretto Giordano, Rebeca Cordeiro Justino, Karina Keller Marques da Costa Flaiban, et al.
    Translation and Interpreting, 2017
  • Methods and advances in Semen analysis
    Biotechnology of Animal Reproduction, 2016
  • Evaluation of physiological parameters of dogs submitted to uterine lymphatic mapping using patent blue V dye for anaphylaxis detection
    Rebeca C. Justino, Guilherme S. Cardoso, Luiz G.C. Trautwein, Rubens I.A. Alves, Karina K.M.C. Flaiban, et al.
    Pesquisa Veterinaria Brasileira, 2016
  • Prevalence of different cytomorphological types of transmissible venereal tumours and the association with prognosis in dogs treated with vincristine sulphate - Retrospective study
    Cristiane Sella Paranzini, Marcos César Sant’anna, Giovana Wingeter di Santis, Maria Isabel Mello Martins
    Semina Ciencias Agrarias, 2015
  • Aflatoxins ingestion and canine mammary tumors: There is an association?
    M.S. Frehse, M.I.M. Martins, E.Y.S. Ono, A.P.F.R.L. Bracarense, L.Y. Bissoqui, et al.
    Food and Chemical Toxicology, 2015
  • Population estimate of the preantral follicles and frequency of multioocyte follicles in prepubertal and adult bitches
    N.T. Lunardon, K.C. Silva-Santos, R.C. Justino, G.T. Dessunti, M.M. Seneda, et al.
    Theriogenology, 2015
  • Hemangiopericytoma in a female dog with direct invasion of abdominal cavity and pulmonary metastasis
    Elisângela Olegário da Silva, Fernanda Romero, Kerriel Thandile Green, Maria Isabel Mello Martins, Ana Paula Frederico Rodrigues Loureiro Bracarense
    Ciencia Rural, 2014
  • Prognostic markers of canine pyometra
    M.C. Sant'Anna, L.G.P. Giordano, K.K.M.C. Flaiban, E.E. Muller, M.I.M. Martins
    Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2014
  • Cryopreservation of bovine spermatozoa from the epididymal tail using conventional and automated methods
    A Ribeiro-Peres, L Munita-Barbosa, M Yumi-Kanazawa, MI Mello-Martins, F Ferreira de Souza
    Archivos De Medicina Veterinaria, 2014
  • Uterine lymphangiography: Comparison of two methods for locating the medial iliac lymph node
    Rebeca C. Justino, Guilherme S. Cardoso, Luiz G.C. Trautwein, Gabriel T. Dessunti, Deise V. Oliveira, et al.
    Pesquisa Veterinaria Brasileira, 2014
  • Ectopic pregnancy in a queen: A report case
    Acta Veterinaria Brasilica, 2013
  • Comparison of Two Different Extenders for Cryopreservation of Epididymal Dog Sperm
    MIM Martins, RC Justino, MC Sant'Anna, LGC Trautwein, FF Souza
    Reproduction in Domestic Animals, 2012
  • Cryopreservation of feline epididymal sperm using Botu-crio® extender after cooling for 24h in Botu-tainer® semen transportation container
    Acta Veterinaria Brasilica, 2012
  • Clinical approach to canine vaginitis
    Marcos Cezar Sant’Anna, Andrei Kelliton Fabretti, Maria Isabel M Martins
    Semina Ciencias Agrarias, 2012
  • Osteopontin in seminal plasma and sperm membrane of dogs
    FF Souza, VH Chirinéa, MIM Martins, MD Lopes
    Reproduction in Domestic Animals, 2009
  • Fertilizing capacity of frozen epididymal sperm collected from dogs
    MIM Martins, LC Padilha, FF Souza, MD Lopes
    Reproduction in Domestic Animals, 2009
  • Vasectomy effect on canine seminal plasma biochemical components and their correlation with seminal parameters
    Fabiana Ferreira de Souza, Maria Isabel Mello Martins, Maria Denise Lopes
    Theriogenology, 2006
  • Morphological and functional characteristics of chilled semen obtained from domestic feline epididymides (Felis catus)
    A.I. Silva Balbin Villaverde, M.I. Mello Martins, V. Basto Castro, M.D. Lopes
    Theriogenology, 2006
  • Heparin-binding proteins of canine seminal plasma
    Fabiana Ferreira de Souza, Maria Isabel Mello Martins, Carlos Eurico dos Santos Fernandes, Paulo Eduardo Martins Ribolla, Maria Denise Lopes
    Theriogenology, 2006
  • Cryopreservation effects on domestic cat epididymal versus electroejaculated spermatozoa
    J.M. Tebet, M.I.M. Martins, V.H. Chirinea, F.F. Souza, D. Campagnol, et al.
    Theriogenology, 2006