Marine-Derived Biosurfactant-Producing Bacteria for Bioremediation of Oil-Contaminated Seawater: Isolation, Optimization, and Application Appavoou Valli, Jayabalan Jayaprakash, Baranidharan Paramanathan Journal of Pure and Applied Microbiology, 2025 Marine oil pollution is a persistent environmental hazard that necessitates eco-compatible remediation strategies. This study reports the isolation and identification of biosurfactant-producing bacterial strains from oil-contaminated seawater collected from Ennore Harbor, Chennai, India. A total of 21 morphologically distinct isolates were obtained and evaluated for their biosurfactant-producing abilities using CTAB (Cetyl Tri Ammonium Bromide) agar, hemolysis, drop collapse, oil spreading, and emulsification index (E24) assays. Among the isolates, Bacillus cereus Is15 exhibited the highest and most consistent biosurfactant activity. Molecular identification via 16S rRNA sequencing revealed Is15 as Bacillus cereus Is15. Optimization studies indicated that peptone and glucose, at pH 7 and 37 °C, significantly enhanced the biosurfactant yield (0.120 g) and crude oil degradation (1.084 g). GC-MS (Gas Chromatography-Mass Spectrometry) analysis indicated the presence of compounds with surface-active properties, including isopropyl myristate and tetradecanoic acid. The application of the biosurfactant in a laboratory-scale oil degradation assay mimicking marine conditions resulted in a substantial reduction in total petroleum hydrocarbons (1.186 g), comparable to that of sodium dodecyl sulfate (SDS; 1.512 g). These findings highlight the potential of B. cereus Is15-derived biosurfactants as efficient and environmentally sustainable agents for marine oil bioremediation.
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Marine-Derived Biosurfactant-Producing Bacteria for Bioremediation of Oil-Contaminated Seawater: Isolation, Optimization, and Application. A Valli, J Jayaprakash, B Paramanathan Journal of Pure & Applied Microbiology 19 (4) , 2025 2025
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MOST CITED SCHOLAR PUBLICATIONS
Phytogenic zinc oxide nanoparticles (ZnO NPs) using Solanum xanthocarpum fruit extract: Its evaluation of photocatalytic activity, molecular docking and biomedical applications J Jayabalan, S Paramasivam, C Jayaseelan, B Paramanathan, G Mani, ... Inorganic Chemistry Communications 173, 113792 , 2025 2025 Citations: 20
Systematic antimicrobial, biofilm, free radical inhibition and tyrosinase inhibition assessments of efficient green silver nanoparticles from the aqueous root extract of … K Kaliappan, P Nagarajan, J Jayabalan, H Pushparaj, S Elumalai, ... RSC Pharmaceutics 2 (1), 147-162 , 2025 2025 Citations: 16
Molecular characterization and molecular docking of xylanase produced by novel species Bacillus aryabhattai isolated from mangrove soil and its biodegradation efficiency J Jayaprakash, D Johnmilton, M Ganesh, C Swaminathan, ... Biocatalysis and Agricultural Biotechnology 66, 103582 , 2025 2025 Citations: 5
Multifunctional activities of phytogenic ZnO nanoparticles mediated Anacardium occidentale: In-silico insights into Staphylococcus aureus biofilm-associated protein (BAP) B Paramanathan, C Jayaseelan, R Gopal, S Sekar, JP Ebenezer, ... Journal of Molecular Structure, 144994 , 2025 2025 Citations: 2
Microbial fabrication of selenium nanoparticles and bacterial cellulose-SeNPs nanocomposite for multifunctional applications B Paramanathan, C Jayaseelan, K Dhanabalan, S Sekar, HT Jang, ... Materials Chemistry and Physics, 131891 , 2025 2025 Citations: 2
Phyto-engineered selenium nanoparticles from Origanum majorana: Synthesis, physicochemical characterization, in vitro biomedical evaluation, and molecular docking against HPV … C Jayaseelan, B Paramanathan, C Kamaraj, S Bhoopathy, B Jayaraman, ... Inorganic Chemistry Communications, 116671 , 2026 2026
Marine-Derived Biosurfactant-Producing Bacteria for Bioremediation of Oil-Contaminated Seawater: Isolation, Optimization, and Application. A Valli, J Jayaprakash, B Paramanathan Journal of Pure & Applied Microbiology 19 (4) , 2025 2025