I have obtained my Ph.D., degree in full time (Biochemistry), Bharathidasan University, India. I have 16 Years Teaching experience (Malaysia & India) and 3 Years postdoctoral experience (South Korea & Malaysia) in the Applied Science department.
So far, I have published 69 research papers (SCOPUS and Web of Science Journal), 28 book chapters published at the international level. Following that, I have guided Ph.D., Scholar 12; of that 4 research scholars were awarded. In addition, I am acting as the Examiner in many Scopus journals.
I have the potential to write a research proposal to a National and International funding agency. In addition, to get funding from various sources, to organize the National, International conferences, and the Faculty Development Program, Work shop, Project Hackathon (School Students), Industry Internship, Industrial Visit, Patent (Design & Utility). I have the ability to organize the Industry-Academia Meet, MOU with various industry.
EDUCATION
ACADEMIC QUALIFICATION:
Ph.D., Full time (2008)– Biochemistry, BHARATHIDASAN UNIVERSITY, INDIA
M.Sc., Full Time (1999)–Biochemistry, BHARATHIDASAN UNIVERSITY, INDIA
B.Sc., (1997) – Chemistry, BHARATHIDASAN UNIVERSITY, INDIA
RESEARCH, TEACHING, or OTHER INTERESTS
Cancer Research, Multidisciplinary, Plant Science, Clinical Biochemistry
Stem Cell Therapies Approach for Treating Cancers: Opportunity Progress and Challenges Vijay Lobo, Bakrudeen Ali Ahmed Abdul, Shenbagavarshini Sivasankar, Abdul Hakeem K, Mahmood Pasha, Ram Arun Kumar Biomedical and Pharmacology Journal, 2024 For the past few years cancer is the second most primary reason for death among human. There are several treatment techniques are raised till now. But they all have side effects and they do not completely eradicate the cancer. Therapy resistant and progression of cancer are frequently caused by the inadequate and generalized targets of conventional therapeutic techniques in the treatment of cancer. Hence, the treatment of the cancer has a challenging one. Over the last decade, stem cell based therapy techniques have become increasingly appealing treatment choices. Although stem cells can be used for regenerative therapy, therapeutic transporters for drugs, biomedical applications, drug targeting and immune cell production, they also show remarkable biological behaviors such as self-renewal, direct motility, differentiating, and immuno regulatory methods. Stem cells are recently being used as delivery vehicles for a variety of specific proteins and viruses, particularly in cancer therapy. The goal of this chapter is i). To highlight a number of research that have effectively used these techniques to combat distinct cancer kinds; ii) With a focus on a variety of factors which are crucial to the victory of future in cancer stem cell therapy and; iii) Focuses on stem cells, is types, properties and stem cell therapies.
Estimating Anticancer Effects of Yohimbine in DMBA-Induced Oral Carcinogenesis Hamster Model: Utilizing Biochemical and Immunohistochemical Techniques Nasimudeen R. Jabir, Shams Tabrez, Nojood Altwaijry, Mohd Shahnawaz Khan, Arun Kumar Ramu, Bakrudeen Ali Ahmed Cell Biochemistry and Function, 2024 Yohimbine is a potent bioactive indole alkaloid, isolated from a variety of biological sources and has long been used as a natural stimulant and aphrodisiac, particularly to treat erectile dysfunction. However, some literature also points toward its anticancer effect in different experimental models. The current study aimed to address a clinical concern on the therapeutic utilization of yohimbine as a repurposed drug. We employed 7,12‐dimethylbenz[a]anthracene (DMBA)‐induced hamster buccal pouch carcinogenesis model juxtaposed with biochemical investigation of several detoxification and antioxidant markers, such as Cyt p450, Cyt b5, thiobarbituric acid reactive substance (TBARS), glutathione (GSH), glutathione reductase (GR), glutathione S transferase (GST), DT‐diaphorase, vitamin C, vitamin E, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). The immunohistochemical assessment of cyclooxygenase‐2 (COX‐2), interleukin‐6 (IL‐6), proliferating cell nuclear antigen (PCNA), and cyclin D1 expression were also performed to observe the effect of yohimbine on these markers. The hamsters treated with DMBA presented the growth of tumors in the buccal pouches, accompanied by significant changes in the liver and buccal mucosa levels of Phase I & II detoxification enzymes and lipid peroxidation (LPO). A significant rise in the range of 2‐ to 3.5‐fold was observed in Cyt p450, Cyt b5, and LPO in DMBA‐treated animals. However, oral administration of yohimbine significantly restored the LPO, antioxidant, and detoxifying enzyme activities. Additionally, the levels of COX‐2, IL‐6, PCNA, and cyclin D1 were also found to be downregulated by yohimbine treatment. In conclusion, yohimbine improved the biochemical and immunohistochemical markers of DMBA‐induced oral cancer and reverted to near normal values via ameliorating the underlying inflammation and oxidative stress conditions. Our study highlighted the potential of yohimbine as anticancer agent, especially against oral cancer and suggested its possible use as repurposed drug.
Potential Secondary Metabolites and Medical Application of Marine Larvae J. Jeyasrree, J. Jasmine Buelah, Abdul Bakrudeen Ali Ahmed, Sumathy Rengarajan Marine Larvae Developments and Applications, 2024 A unique source in the world’s oceans, the deep ocean and the geothermal vents, is now becoming the focus of considerable microbiological interest. Marine micro biota does not only lead in quantitative measures, but also has tremendous potential to represent a biomedical resource of unknown magnitude and great promise. Presence of obligate barophiles, bacteria which require pressures as high as 600 atmospheres for growth, has been reported recently from the deep sea environment. As certain classes of microbes have unique adaptations for high salt environments and high hydrostatic pressure, the immense miscellany of the microorganisms in marine habitats is quite perceptible. In addition, Inagaki et al. (2006) reported that previously unidentified prokaryotic communities such as the JS1 and DSAG groups occur widely in organic rich deep marine sediments associated with methane hydrates along the Pacific Ocean margin.
Sinapic Acid Improves Neurotransmitter Status and Oxidative Stress-Related Changes in Mouse Model of Experimental Parkinsonism Preeja Prabhakar, Bakrudeen Ali Ahmed, Saravana Babu Chidambaram, Arun Kumar Ramu, Arjun Pandian International Journal of Nutrition Pharmacology Neurological Diseases, 2023 Parkinsonism is a progressive neurodegenerative disease of the dopaminergic neurons in the substantia nigra. Oxidative stress is a known causative factor in the death of dopaminergic neurons. Sinapic acid (SNP or SA), a hydroxycinnamic type of the abundantly available plant-derived metabolite, is known for its antioxidant and anti-inflammatory properties. In the present study, we assessed the effects of sinapic acid against 1-methyl-4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) induced Parkinson disease (PD) in a mouse model. The animals were pretreated with vehicle or SNP for a period of 5 days. On Day 5, 45 minutes after giving sinapic acid (SNP/SA) or normal saline (control/vehicle/), MPTP was administered. Motor function was tested using a beam walk test. Dopamine level was measured by HPLC. SNP-pretreated mice showed significant improvement in the levels of dopamine at the striatum and substantia nigra pars compacta regions of the brain. SNP also showed improved antioxidants like glutathione and superoxide dismutase levels. These were also corroborated by the improvement in motor function tests. Sinapic acid, with its antioxidant ability and dopamine-replenishing properties attenuated the pathological features associated with MPTP intoxicated dopaminergic degeneration in experimental PD.
Prioritization of bioactive compounds envisaging yohimbine as a multi targeted anticancer agent: insight from molecular docking and molecular dynamics simulation Nasimudeen R. Jabir, Md Tabish Rehman, Mohamed F. AlAjmi, Bakrudeen Ali Ahmed, Shams Tabrez Journal of Biomolecular Structure and Dynamics, 2023 Recently, multi-targeted drugs have attracted much attention in cancer therapy where several therapeutic proteins are targeted by a single agent. Using the published scientific literature, we selected sixteen well-known anticancer targets and seven potential phytobioactive chemicals to find a multitargeted compound by screening through molecular docking. The feasible protein-ligand interaction was further predicted by protein-ligand interaction analysis and molecular dynamic simulation. The phytochemical yohimbine exhibited the lowest docking score in the range of -8.3 to -10.0 kcal/mol over other ligands with all the studied protein targets. Molecular interaction data also revealed the feasible binding of yohimbine with all targets. Moreover, the molecular simulation data also confirmed the stability of protein-ligand complexes with three most scored targets viz. ERK2, PARP1 and PIK3α. Based on our results, yohimbine seems to be the most potent compound out of those selected compounds and can be considered as effective lead molecule against the studied target proteins.Communicated by Ramaswamy H. Sarma.
In Vitro Ameliorative Effects of Sinapic Acid on Parkinson Related Neurotoxicity in SHSY5Y Cell Lines Preeja Prabhakar, Bakrudeen Ali Ahmed, Saravana Babu Chidambaram, Arun Kumar, Arjun Pandian International Journal of Nutrition Pharmacology Neurological Diseases, 2023 The neuroprotective effects of polyphenols have been reported in the prevention of the early onset or delay of the progression of various neurodegenerative diseases, including Parkinson disease (PD). Neuroinflammation, oxidative stress, and mitochondrial dysfunction play significant roles in the pathophysiology of PD. Sinapic acid (SNP) is a naturally occurring polyphenol belonging to a group of hydroxycinnamic acids, which has gained importance owing to its beneficial effects, including antioxidant and anti-inflammatory properties. The present study aimed to develop an insight into the effects of sinapic acid on mitigating the inflammatory markers, oxidative stress, and deranged mitochondrial dynamics in human neuroblastoma cells (SHSY5Y) intoxicated with MPP+. The modulating variations of SNP on apoptosis, mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS), and expression of proteins like PARKIN, PINK1, DJ-1, Bax, and BCl2 were analyzed in MPP+ induced PD-like toxic conditions. Pre-treatment with SNP decreased the levels of ROS and improved MMP. Also, SNP down-regulated the expression of PARKIN1, caspase-3, and DJ-1, along with a reduction in the expression of inflammatory markers such as IL-1β and TNF-α. Further, SNP was observed to increase the levels of BCl2, an anti-apoptotic protein, and the activity of superoxide dismutase (SOD), an enzymatic antioxidant. Based on the above results, the authors concluded that SNP exhibited neurotherapeutic potential in PD-like neurotoxic conditions. The present study reported the preclinical and mechanistic approach to identify the exact mechanism of action of SNP in PD.
Marine algal secondary metabolites are a potential pharmaceutical resource for human society developments Somasundaram Ambiga, Raja Suja Pandian, Lazarus Vijune Lawrence, Arjun Pandian, Ramu Arun Kumar, Bakrudeen Ali Ahmed Abdul Marine Biochemistry Applications, 2022 The oceans are likely the most important natural resource on the planet, supplying chemical compounds that have potential to develop in industry as drugs, beauty products, food supplements, genetic probes, enzymes, active pharmaceutical ingredients and agricultural products. Many bioactive compounds are isolated from marine sources such as polysaccharides, alkaloids, peptides and polyphenols. Phlorotannin, gallic acid, quercetin, phloroglucinol, carotenoids and its derivatives are the most studied phytochemicals in algae. Polyphenol compounds such as phenolic acids, flavonoids, epigallate, epicatechin, anthocyanidins, gallic acids, tannins and catechin have much potential to reduce the mitotic index and decrease the cellular proteins were are needed for cancer cell proliferation and colony formation. Proteolytic enzymes, which are synthesized by marine species, have a broad range of pharmaceutical applications, including digestive medicines and new anti-inflammatory agents which protect the body from cancer, making them effective anti-cancer medicines. Scientists have identified an increasing number of marine microbes capable of producing enzymes such as amylase and lipase and chitinases. A diet rich in marine items is expected to result in a lower incidence of diabetes, cancer and obesity. Fucoxanthin, fucosterol, sulfated polysaccharides and phlorotannins have been detected in the majority of the brown algae species, with the highest potential applications as an antidiabetic agent. Marine bacteria produce many secondary metabolites such as topsentins, manoalide and pseudopterosins which shows high anti-inflammatory activities, and bryostatins, discodermolide, and sarcodictyin exhibit anticancer activities, while marinone shows high antibiotic activity. In addition, it is equally important to raise public awareness and focus on marine products to develop a more readily available and complete alternative to daily food with medicinal effects.
A literature perspective on the pharmacological applications of yohimbine Nasimudeen R. Jabir, Chelapram K. Firoz, Torki A. Zughaibi, Mohammed Abdullah Alsaadi, Adel M. Abuzenadah, Ahmed Ibrahim Al-Asmari, Ahdab Alsaieedi, Bakrudeen Ali Ahmed, Arun Kumar Ramu, Shams Tabrez Annals of Medicine, 2022
COVID-19 and cancer comorbidity: Therapeutic opportunities and challenges Anup S. Pathania, Philip Prathipati, Bakrudeen AA. Abdul, Srinivas Chava, Santharam S. Katta, Subash C. Gupta, Pandu R. Gangula, Manoj K. Pandey, Donald L. Durden, Siddappa N. Byrareddy, Kishore B. Challagundla Theranostics, 2020
Sucrose enhanced stigmasterol production in callus cultures of Wedelia biflora (L.) D.C Philippine Agricultural Scientist, 2018
Buffering capacity as an indicator of the cariogenic potential International Journal of Civil Engineering and Technology, 2017
The role of bone resorption markers in early detection of osteoporosis in perimenopausal women of mangalore International Journal of Civil Engineering and Technology, 2017
Seaweed Polysaccharides and Their Production and Applications Abdul Bakrudeen Ali Ahmed, Mohaddeseh Adel, Ali Talati, Muthusamy S. Kumar, Kamaludeen Abdulrahim, Meera M. Abdulhameed Seaweed Polysaccharides Isolation Biological and Biomedical Applications, 2017
Effect of adenine, sucrose and plant growth regulators on the indirect organogenesis and on in vitro flowering in Begonia x hiemalis fotsch. Australian Journal of Crop Science, 2013
Evaluation of yield and morphology traits in 72 genotypes of sainfoin (onobrychis viciifolia scop) through factor analysis Legume Research, 2012
Different wavelengths light to induce physiological changes callus for the biosynthesis of gymnemic acid in Gymnema sylvestre Agro Food Industry Hi Tech, 2012
Lead effect and influence of medicinal plants mixed feeds on acetylcholinesterase activity of fresh water fish Oreochromis mossambicus Biomedicine India, 2009
Stem cell treatment and its curative value Biomedicine India, 2009
Role of in vivo leaf and in vitro callus of Gymnema sylvestre in maintaining the normal levels of blood glucose and lipid profile in diabetic Wistar rats Biomedicine, 2008
Stem Cell Therapies Approach for Treating Cancers: Opportunity Progress and Challenges V Lobo, BAA Abdul, S Sivasankar, A Hakeem, M Pasha, RA Kumar Biomedical and Pharmacology Journal 17 (4), 2629-2642 , 2024 2024
Estimating Anticancer Effects of Yohimbine in DMBA‐Induced Oral Carcinogenesis Hamster Model: Utilizing Biochemical and Immunohistochemical Techniques NR Jabir, S Tabrez, N Altwaijry, MS Khan, AK Ramu, BA Ahmed Cell Biochemistry and Function 42 (7), e4132 , 2024 2024
Potential Secondary Metabolites and Medical Application of Marine Larvae J Jeyasrree, JJ Buelah, ABA Ahmed, S Rengarajan Marine Larvae, 238-250 , 2024 2024 Citations: 1
Prioritization of bioactive compounds envisaging yohimbine as a multi targeted anticancer agent: insight from molecular docking and molecular dynamics simulation NR Jabir, MT Rehman, MF AlAjmi, BA Ahmed, S Tabrez Journal of Biomolecular Structure and Dynamics 41 (20), 10463-10477 , 2023 2023 Citations: 7
Transient Gene Expression in Molecular Farming and Functional Genomics of Tea ( Camellia sinensis ): A Review S Mohajer, RM Taha, S Hajivand, M Pirkhezri, ABA Ahmed Journal of Plant Growth Regulation 42 (7), 4000-4019 , 2023 2023 Citations: 6
Sinapic acid improves neurotransmitter status and oxidative stress-related changes in mouse model of experimental parkinsonism P Prabhakar, BA Ahmed, SB Chidambaram, AK Ramu, A Pandian International Journal of Nutrition, Pharmacology, Neurological Diseases 13 … , 2023 2023 Citations: 2
In vitro ameliorative effects of sinapic acid on Parkinson related neurotoxicity in SHSY5Y cell lines P Prabhakar, BA Ahmed, SB Chidambaram, A Kumar, A Pandian International Journal of Nutrition, Pharmacology, Neurological Diseases 13 … , 2023 2023 Citations: 8
Anticancer potentiated bioactive compounds from marine flora AK Ramu, R Rajendran, AM Sivalingam, VD Seshadri, ABA Ahmed Marine Antioxidants, 421-432 , 2023 2023 Citations: 6
A literature perspective on the pharmacological applications of yohimbine NR Jabir, CK Firoz, TA Zughaibi, MA Alsaadi, AM Abuzenadah, ... Annals of medicine 54 (1), 2849-2863 , 2022 2022 Citations: 81
Anticancer potential of yohimbine in drug-resistant oral cancer KB-ChR-8–5 cells NR Jabir, MS Khan, NO Alafaleq, H Naz, BA Ahmed Molecular Biology Reports 49 (10), 9565-9573 , 2022 2022 Citations: 10
Future Trends in Biomedical Applications S Ambiga, RS Pandian, ABA Ahmed, R Ramasubbu, RA Kumar, ... Materials Development and Processing for Biomedical Applications, 297-309 , 2022 2022
Marine algal products and algal nanoparticle synthesis against cancer A Ramu, BA Ahmed Bioprospecting algae for nanosized materials, 261-274 , 2022 2022 Citations: 3
In vitro propagation of Ananas comosus L. Merr.(Morris) H Elias, RM Taha, H Sani, NF Khamarul Huda1, BA Ahmed, S Abdullah, ... VIII South-Eastern Europe Symposium on Vegetables and Potatoes 1320, 319-326 , 2021 2021 Citations: 1
In silico screening of glycogen synthase kinase-3β targeted ligands against acetylcholinesterase and its probable relevance to Alzheimer’s disease NR Jabir, S Shakil, S Tabrez, MS Khan, MT Rehman, BA Ahmed Journal of Biomolecular Structure and Dynamics 39 (14), 5083-5092 , 2021 2021 Citations: 57
Identification of butyrylcholinesterase and monoamine oxidase B targeted ligands and their putative application in alzheimer’s treatment: a computational strategy NR Jabir, MT Rehman, S Tabrez, RF Alserihi, MF AlAjmi, MS Khan, ... Current Pharmaceutical Design 27 (20), 2425-2434 , 2021 2021 Citations: 25
An efficient plant regeneration, detection and identification of secondary metabolites from propagate plants of Peperomia pellucida (L.) for mass cultivatione AB Ali Ahmed, T Lydia, M Muhamed Musthafa, R Mat Taha, ... State natural history museum nas Ukraine , 2021 2021
Saffron as a natural food colorant and its applications ABA Ahmed, RM Taha, N Anuar, H Elias, S Abdullah, A Khan, V Lobo, ... Saffron, 221-239 , 2021 2021 Citations: 15
Reserpine inhibits DNA repair, cell proliferation, invasion and induces apoptosis in oral carcinogenesis via modulation of TGF-β signaling AK Ramu, D Ali, S Alarifi, MHS Abuthakir, BAA Abdul Life sciences 264, 118730 , 2021 2021 Citations: 35
Effect of Biochar Addition to Soil on Plant Quality ABA Ahmed, M Adel, A Talati, V Lobo, VD Seshadri Environmental Biotechnology Vol. 3, 233-242 , 2020 2020
Genomic and Proteomics in Pharmaceutical Products Development and Biological Applications of Marine Derivatives ABA Ahmed, M Adel, P Karimi, T Mahmoudi, R Farzinebrahimi, RA Kumar Encyclopedia of Marine Biotechnology 3, 1867-1874 , 2020 2020
MOST CITED SCHOLAR PUBLICATIONS
Biosynthesis, characterisation and anti-bacterial effect of plant-mediated silver nanoparticles using Artemisia nilagirica M Vijayakumar, K Priya, FT Nancy, A Noorlidah, ABA Ahmed Industrial crops and products 41, 235-240 , 2013 2013 Citations: 509
Chitin, chitosan, oligosaccharides and their derivatives: biological activities and applications SK Kim CRC Press , 2010 2010 Citations: 440
Marine Phytochemical Compounds and Their Cosmeceutical Applications BAARM Taha CRC Press , 2011 2011 Citations: 267
In vitro callus and in vivo leaf extract of Gymnema sylvestre stimulate β-cells regeneration and anti-diabetic activity in Wistar rats ABA Ahmed, AS Rao, MV Rao Phytomedicine 17 (13), 1033-1039 , 2010 2010 Citations: 195
Pharmaceutical, cosmeceutical, and traditional applications of marine carbohydrates ABA Ahmed, M Adel, P Karimi, M Peidayesh Advances in food and nutrition research 73, 197-220 , 2014 2014 Citations: 153
Antidiabetic activity of leaf and callus extracts of Aegle marmelos in rabbit S Arumugam, S Kavimani, B Kadalmani, ABA Ahmed, MA Akbarsha, ... Sci Asia 34 (3), 317-321 , 2008 2008 Citations: 135
Encyclopedia of marine biotechnology SK Kim John Wiley & Sons , 2020 2020 Citations: 115
Properties, Biological Advantages and Industrial Significance of Marine Peptides AB Ali Ahmed, M Vijayakumar, R Pallela, N Abdullah, RM Taha Marine Proteins and Peptides: Biological Activities and Applications, 631-640 , 2013 2013 Citations: 114
Seaweed Polysaccharides: isolation, biological and biomedical applications J Venkatesan, S Anil, SK Kim Elsevier , 2017 2017 Citations: 105
Cardioprotective activity of polysaccharides derived from marine algae: An overview V Mayakrishnan, P Kannappan, N Abdullah, ABA Ahmed Trends in Food Science & Technology 30 (2), 98-104 , 2013 2013 Citations: 87
A literature perspective on the pharmacological applications of yohimbine NR Jabir, CK Firoz, TA Zughaibi, MA Alsaadi, AM Abuzenadah, ... Annals of medicine 54 (1), 2849-2863 , 2022 2022 Citations: 81
Optimizing the fermentation conditions and enhanced production of keratinase from Bacillus cereus isolated from halophilic environment S Arokiyaraj, R Varghese, BA Ahmed, V Duraipandiyan, NA Al-Dhabi Saudi Journal of biological sciences 26 (2), 378-381 , 2019 2019 Citations: 80
Essential oil compositions and cytotoxicity from various organs of Eucalyptus camaldulensis RM Mubarak, E.E, Ali, L.Z, Ahmed, I.F.A, Ahmed, A.B.A, Taha International Journal of Agriculture and Biology 17 (2), 320-326 , 2015 2015 Citations: 70
Effect of picloram, additives and plant growth regulators on somatic embryogenesis of Phyla nodiflora (L.) Greene ABA Ahmed, AS Rao, MV Rao, RM Taha Brazilian Archives of Biology and Technology 54 (1), 7-13 , 2011 2011 Citations: 63
In silico screening of glycogen synthase kinase-3β targeted ligands against acetylcholinesterase and its probable relevance to Alzheimer’s disease NR Jabir, S Shakil, S Tabrez, MS Khan, MT Rehman, BA Ahmed Journal of Biomolecular Structure and Dynamics 39 (14), 5083-5092 , 2021 2021 Citations: 57
In vitro callus induction and plant regeneration of Celosia argentea-an important medicinal plant DA Bakar, BA Ahmed, RM Taha Brazilian Archives of Biology and Technology 57 (6), 860-866 , 2014 2014 Citations: 49
In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions AB Ali Ahmed, AS Rao, MV Rao Protocols for in vitro cultures and secondary metabolite analysis of … , 2009 2009 Citations: 43
Optimized conditions for callus induction, plant regeneration and alkaloids accumulation in stem and shoot tip explants of Phyla nodiflora ABA Ahmed, R Pallela, AS Rao, MV Rao, RM Taha Spanish Journal of Agricultural Research 9 (4), 1262-1270 , 2011 2011 Citations: 39
Reserpine inhibits DNA repair, cell proliferation, invasion and induces apoptosis in oral carcinogenesis via modulation of TGF-β signaling AK Ramu, D Ali, S Alarifi, MHS Abuthakir, BAA Abdul Life sciences 264, 118730 , 2021 2021 Citations: 35