Whitening toothpastes for coffee and wine stains: Impact on enamel color and roughness American Journal of Dentistry, 2026
PEEK surface treatments for resin bonding: A systematic review and network meta-analysis of in vitro studies Rafael Pino Vitti, Simone Gomes de Oliveira, Vanessa Gallego Arias Pecorari, William Cunha Brandt, Débora Alves Nunes Leite Lima, Rafael Rocha Pacheco, Flávio Henrique Baggio Aguiar Dental Materials, 2026 To evaluate and rank surface treatments for bonding resin composites to polyetheretherketone (PEEK) in dental applications using network meta-analysis. Following PRISMA 2020 guidelines and the PRISMA extension for network meta-analyses (registered: OSF https://doi.org/10.17605/OSF.IO/JGRDE ), the research question was structured using the PICOS framework. Eligible studies were in vitro, published in English, Portuguese, or Spanish, with no date restriction. Exclusion criteria included non-dental use of PEEK, absence of surface treatment, lack of control group, use of cleaning methods affecting adhesion, bond strength assessed only after aging, bond strength not tested between PEEK and resin composite, and results not expressed in MPa. Risk of bias was assessed using the RoBDEMAT tool. Data were pooled from studies using comparable surface treatments with different parameters. Nine databases (MEDLINE, Embase, LILACS, BBO, Google Scholar, ProQuest, and institutional repositories) were searched up to February 2025. Study selection: A total of 1809 records were screened, leading to 14 included studies published between 2013 and 2024, evaluating 18 surface treatment protocols comprising 1186 observations. Sulfuric acid combined with manufacturer-recommended bonding agents (P-score: 0.97) was the most effective protocol for enhancing bond strength between PEEK and resin composites, followed by laser treatment with adhesive. Most studies presented moderate risk of bias. Surface treatments require adhesive application to achieve optimal bonding performance. • First network meta-analysis ranking PEEK surface treatments for resin bonding. • Sulfuric acid with visio.link achieved highest bond strength (P-score: 0.97). • Surface treatments require adhesive application for optimal PEEK bonding. • Laser and sandblasting with adhesives offer safer chairside alternatives. • Clinical validation studies needed to confirm in vitro rankings.
Graphene–Catechol Dental Sealant: Antibacterial and Mechanical Evaluation Renata Pereira, Flávio H. B. Aguiar, Rodrigo B. E. Lins, Maria C. A. J. Mainairdi, Bruna G. Silva, Marcela A. Ferretti, Klaus Rischka Advanced Engineering Materials, 2025 This study evaluates the antibacterial activity, bond strength, fracture pattern, nanohardness, indentation modulus, indentation creep, and elastic part of indentation work of three experimental dental sealants: Control; G (Control with 0.25% graphene); and Gd (Control with 0.25% graphene modified by 3,4‐dihydroxyphenyl‐L‐alanine). For antibacterial activity, samples ( n = 10) are incubated with Streptococcus mutans ( S. mutans ) or Lactobacillus casei ( L. casei ). S. mutans colony‐forming units (CFUs) growth is statistically analyzed; L. casei CFUs growth is analyzed descriptively. Bond strength is tested by shear of sealant pillars on enamel ( n = 8). Mechanical tests are performed using a Vickers indenter initially ( T 0 ) and after water storage ( T 1 ) ( n = 8). Control shows the highest S. mutans CFUs (100%). G and Gd show 57.2% and 67.0% reductions, respectively, with no statistical difference. L. casei growth decreases as follows: Control > G > Gd. Bond strength is similar across groups (≈16.6 MPa), with predominantly adhesive failures. In general, at both time points, Gd shows the highest nanohardness and indentation modulus (e.g., 15.05 HV and 0.62 MPa higher than Control and G, respectively, at T 1 ). Control has the highest creep; G and Gd shows the highest elastic part of indentation work. Only Control's nanohardness declines from T 0 to T 1 . Overall, Gd demonstrates promising antibacterial and mechanical performance.
Do bleaching dentifrices associated with a low-concentration hydrogen peroxide gel affect the surface properties and mineral content of enamel? Maiara Rodrigues de Freitas, Kusai Baroudi, Giuseppe Minervini, Ana Carolina Andreucci, Maria Júlia de Carvalho Feijó de Moura, Rodrigo Barros Esteves Lins, Flavio Henrique Baggio Aguiar, Rayssa Ferreira Zanatta, Priscila Christiane Suzy Liporoni Dental and Medical Problems, 2025 BACKGROUND: Comparing the new and existing products is essential to identify the one that minimizes risks to the dental structures while effectively fulfilling its intended purpose. OBJECTIVES: The aim of the present study was to evaluate possible changes in the surface properties, mineral loss and color of bovine enamel subjected to bleaching dentifrices used in combination with a low-concentration hydrogen peroxide (HP) bleaching gel. MATERIAL AND METHODS: Bovine tooth substrates disinfected with thymol were used to make 112 circular samples with a diameter of 4 mm. After the samples were embedded in transparent acrylic resin, they were polished with grit of decreasing granulation and divided into 8 groups (n = 14 per group), according to the bleaching treatment (Opalescence Go (OpGo) - 10% HP or immersion in buffered water (BW) - control) and the toothpastes used (OMW - Oral-B 3D Mineral White Clean; CLW - Colgate Luminous White Advanced; STW - Sensodyne True White; or CT - Colgate Total 12). The bleaching gel was used for 30 min daily for 10 days. The samples were brushed using an electric brush and a slurry (3:1 ratio) for 120 s twice a day, with an interval of 12 h, with the first brushing immediately after the bleaching treatment. Prior to the commencement of the treatment, the initial microhardness, surface roughness and color data was evaluated. RESULTS: For microhardness, a reduction in values was observed for all groups, except for the control (CT + salt), whereas for roughness, there was an increase in the final values for all groups. A significant difference in the post-treatment values was observed only for the lightening treatment factor (p = 0.0079). CONCLUSIONS: There was a reduction in enamel microhardness for all groups, except for the group that used a non-bleaching dentifrice and was treated with BW.
Differential expression reveals inflammatory response and oxidative stress genes in dentin caries Simone G. Oliveira, Rodrigo Jardim, Nelson Kotowski, Alberto M.R. Dávila, Hélio R. Sampaio-Filho, Karina G.S. Ruiz, Flávio H.B. Aguiar Archives of Oral Biology, 2025 OBJECTIVE: This study employs RNA-Seq to investigate differentially expressed genes involved in extracellular matrix (ECM) degradation, focusing on collagenases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2). DESIGN: Total RNA from caries and caries-free teeth was extracted from pulp, predentin, and dentin. Samples were sequenced using Illumina® technology. Quality validation was done with FASTQC, and low-quality bases were removed using TRIMMOMATIC. Reads were aligned using SALMON against the human transcriptome (CHR38), followed by quantification using Transcripts Per Million. Differential gene expression analysis was conducted using DESeq2 (FDR < 0.05, |log2FC| ≥ 1). Functional enrichment analyses employed Gene Ontology and KEGG databases. RESULTS: Sequencing produced 16-37 million reads per sample, with an average alignment rate of 88.08 %. A total of 334 differentially expressed genes (DEGs) were identified: 195 upregulated and 139 downregulated. Upregulated genes included SAA1 (log2FC = 2.3, p-adj = 0.001) and ORM1 (log2FC = 2.0, p-adj = 0.002), associated with inflammation. MMP-9 was significantly downregulated (log2FC = -1.8, p-adj = 0.003), while MMP-2 showed higher expression in decayed tissues. TIMP-1 expression increased in decayed dentin; TIMP-2 was upregulated in both decayed and caries-free dentin. Protein interaction analysis identified EGFR and metallothioneins as key acute-phase proteins. CONCLUSIONS: This study reveals the role of inflammatory and oxidative stress-related genes in dentin caries and shows disruption in the ECM degradation-repair balance. Increased MMP-2 and TIMP-1 expression suggests a compensatory response. MMP activity may serve as a therapeutic target to enhance tissue resilience and slow caries progression.
Synthesis, physical properties, and root canal sealing of experimental MTA- and salicylate-based root canal sealers Rafael Pino Vitti, Kusai Baroudi, Tarun Walia, Raghavandra M. Shetty, Flávia Goulart da Rosa Cardoso, Flávia de Moura Pereira, Evandro Piva, Cesar Henrique Zanchi, Gabriel Flores Abuna, Carolina Oliveira de Lima, Emmanuel João Nogueira Leal Silva, Flávio Henrique Baggio Aguiar, Mário Alexandre Coelho Sinhoreti Plos One, 2025 Objectives To develop and evaluate the physical properties and sealing ability within the root canal of three experimental sealers based on MTA and a salicylate resin. Materials and methods The experimental sealers were composed of two pastes. The base paste was prepared using 1,3-butyleneglycol disalicylate and bismuth oxide. Three different catalytic pastes were formulated, creating three groups: [MTA] n,n,dihydroxyethyl-p-toluidine (DPT), titanium dioxide (TiO2), and mineral trioxide aggregate (MTA); [MTA-HA] DPT + TiO2 + MTA + hydroxyapatite (HA); and [MTA-DCPD] DPT + TiO2 + MTA + dibasic calcium phosphate dihydrate (DCPD). MTA Fillapex (Angelus) was used as the commercial reference (control). The sealers were manipulated at a 1:1 ratio (base paste:catalyst). Tests for working time and setting time, flow, and film thickness were conducted following ISO 6876:2012 standards. Single-rooted human teeth root canals were utilized for evaluating root canal filling using micro-computed tomography, push-out bond strength testing, and sealer penetration into dentinal tubules using confocal microscopy. Failure patterns in the push-out test were classified as adhesive, cohesive, or mixed. Sealer micromorphology was analyzed via scanning electron microscopy. Data were analyzed statistically (α = 0.05). Results MTA Fillapex showed the longest working and setting times, highest flow, the lowest film thickness, and better penetration into dentinal tubules. There was no difference in void among evaluated sealers. Overall, MTA-DCPD sealer showed the lowest bond strength values for cervical and apical thirds. Micromorphological analysis revealed similar crystallographic properties among all sealers. Conclusions The sealers tested showed reduced working and setting times, with flow and film thickness according to ISO 6876:2012. Their void volume and bond strength were similar to MTA Fillapex, except for MTA-DCPD. Clinical Relevance: The experimental root canal sealers demonstrated suitable physical properties and good adaptation within the root canal.
The Dentin Microbiome: A Metatranscriptomic Evaluation of Caries-Associated Bacteria Simone G. de Oliveira, Rodrigo Jardim, Nelson Kotowski, Alberto M. R. Dávila, Hélio R. Sampaio-Filho, Karina G. S. Ruiz, Flávio H. B. Aguiar Biomedicines, 2025 Background/Objectives: Dental caries remains a prevalent chronic disease globally, driven by complex interactions between the host, diet, and microbial communities. This study employs a metatranscriptomic RNA-Seq analysis to explore the functional dynamics of the dentin microbiome in both healthy and carious teeth. By examining the transcriptional activity of bacterial communities, we aimed to identify key microbial species and molecular functions associated with caries progression. Methods: Samples from six patients (three healthy and three decayed teeth) were analyzed using the Illumina NovaSeq 2000 platform, with data processed through the SAMSA2 pipeline for taxonomic and functional annotation. Results: The differential expression analysis revealed significant upregulation of Streptococcus and Lactobacillus species, including S. mutans, S. sobrinus, and L. salivarius, in carious samples, highlighting their roles in acid production and carbohydrate metabolism. Additionally, Mycobacterium species, known for their biofilm-forming capabilities and acid tolerance, were upregulated in decayed teeth. The Gene Ontology (GO) enrichment analysis identified unique molecular functions and biological processes in carious teeth, such as carbohydrate metabolism, oxidative stress response, and bacterial cell wall biogenesis, which are critical for microbial survival in acidic environments. In contrast, healthy teeth exhibited functions related to homeostasis and nutrient acquisition, reflecting a balanced microbial community. Conclusions: The study underscores the polymicrobial nature of dental caries, with multiple bacterial species contributing to disease progression through diverse metabolic and stress-response mechanisms. These findings provide deeper insights into the ecological shifts within the oral microbiome during caries development, emphasizing the importance of a functional metatranscriptomic analysis in understanding the pathogenesis of dental caries.
Erosive and abrasive challenge effects on superficial roughness of resin infiltrants and sealant Janaina Cardoso Santos Couto, Janaína Emanuela Damasceno, Gabriela Alves de Cerqueira, Priscila Régis Pedreira, Jade Laísa Gordilio Zago, Flávio Henrique Baggio Aguiar, Giselle Maria Marchi Brazilian Journal of Oral Sciences, 2025 Aim: This study aimed to evaluate changes in surface roughness of an experimental resin infiltrant, in comparison with the commercial infiltrant Icon® and the resin sealant Prevent®, following different erosive challenges and abrasive simulations with brushing cycles. Methods: A total of 20 samples per group were prepared: (I) Commercial Infiltrant Icon® (IC), (II) Experimental Infiltrant (EI), and (III) Commercial Resin Sealant Prevent® (SR). Surface roughness was assessed using a rugosimeter at three distinct time points: prior to the erosive challenge (T0), post-erosive challenge (T1), and following the abrasive simulation (T2). Each group was subdivided into two subgroups (n=10) to undergo different erosive challenges: intrinsic acid and extrinsic acid simulations. The intrinsic acid challenge was conducted by immersing the samples in a hydrochloric acid demineralizing solution (pH=2.3), while the extrinsic acid challenge involved immersion in a soft drink demineralizing solution (pH=2.9). Following the erosive challenges, the specimens were maintained in relative humidity and surface roughness was reassessed (T1). Subsequently, the same groups underwent brushing simulation (10.000 cycles), after which surface roughness was measured again. Data were analyzed by three-way ANOVA with repeated measures, followed by Tukey’s post-hoc test for multiple comparisons, with a significance level set at 5%. Results: All groups demonstrated an increase on surface roughness, regardless of demineralizing method used (p<0.001). The resin sealant exhibited the highest surface roughness changes under both erosive conditions when compared to the resin infiltrants. There was no statistical difference between the two erosive challenges, regardless of the material and time. Conclusion: In conclusion, all three tested materials showed an increase in surface roughness following erosive and abrasive challenges.
Experimental resininfiltrant with antibacterial activity and ionic release: in-vitro study Layla Karine Oliveira Silva, Ana Ferreira Souza, May Anny Alves Fraga, Priscila Regis Matos Pedreira, Américo Bortolazzo Correr, Flávio Henrique Baggio Aguiar, Giselle Maria Marchi Brazilian Journal of Oral Sciences, 2025 Objective: To assess the influence of the combination of the antibacterial monomer dimethylaminohexadecyl methacrylate (DMAHDM) and amorphous calcium phosphate nanoparticles (NACP) on the antibacterial and ion release potentials, as well as the physical properties of experimental resin infiltrants. Methodology: The study comprised the following groups: ERI (Pure Experimental Resin Infiltrant [ERI]: 75% TEGDMA + 25% BisEMA, 0.5% camphorquinone [CQ], and 1% ethyl 4-dimethylaminobenzoate [EDMAB]); ERIDM (ERI + 3% DMAHDM), ERINACP (ERI + 1.5% NACP), and ERIDM_NACP (ERI + 3% DMAHDM + 1.5% NACP). From samples of each group, Degree of Conversion (DC; n=6) and Sorption and Solubility (SO/SOL; n=8) were assessed. The antibacterial potential was evaluated through biomass accumulation (BA; n=6) and bacterial metabolism (BM; n=6) assays after cultivating Streptococcus mutans biofilm on the materials. Ionic release (IR; n=3) of Ca2+ and PO4 (3-) from the groups after 7, 14, and 28 days of immersion was also analyzed. Data were analyzed for normality and homoscedasticity, and statistical analysis was performed using appropriate tests with a significance level of 5%. Results: For DC, ERIDM showed no statistical difference from ERI. ERI had the lowest means of SO/SOL, and ERIDM_NACP had the highest. ERIDM exhibited no statistical difference from ERI. For BM, ERIDM and ERIDM_ NACP had the lowest means. ERINACP and ERIDM_NACP exhibited ionic release during the analyzed period. Conclusions: The resin infiltrant containing DMAHDM and NACP exhibits potent antibacterial activity against S. mutans and Ca2+ and PO4 (3-) ionic release.
Effect of fluoride or chitosan toothpaste and at-home bleaching in enamel roughness, tooth color, and staining susceptibility American Journal of Dentistry, 2024
The in vitro effect of solutions with or without sugar in dental bleaching Isabel Ferreira Barbosa, João Victor Frazão Câmara, Josué Junior Araujo Pierote, Lívia Rodrigues Menezes, Paola Ferreira de Freitas, Lethycia Almeida Santos, Tatiana Martini, Luis Alexandre Maffei Sartini Paulillo, Flavio Henrique Baggio Aguiar Journal of the Mechanical Behavior of Biomedical Materials, 2023
Restoration of teeth with customized CAD/CAM glass fiber posts: report of 2 cases General Dentistry, 2022
Can brushing with regular and whitening dentifrices influence changes in color and micromorphologic surfaces of ceramic brackets subjected to coffee staining? General Dentistry, 2022
PDMS-urethanesil hybrid multifunctional materials: combining CO2 use and sol–gel processing Florian Günther, Elton F. S. Lima, Kelen M. F. Rossi de Aguiar, Jefferson R. Bearzi, Mateus B. Simões, Ricardo Schneider, Rafael A. Bini, Sidney J. L. Ribeiro, Michel Wong Chi Man, Klaus Rischka, Flávio H. B. Aguiar, Renata Pereira, Maria do Carmo A. J. Mainardi, Marina C. Rocha, Iran Malavazi, Tânia A. Passeti, Marcio L. Santos, Hidetake Imasato, Ubirajara Pereira Rodrigues-Filho Journal of Sol Gel Science and Technology, 2020
Chemical and physical evaluation of the luting systems for veneers submitted to accelerated artificial aging Journal of International Dental and Medical Research, 2020
Consumption of different energy beverages and oral health Núbia Inocencya Pavesi Pini, Jéssica Dias Theobaldo, Débora Alves Nunes Leite Lima, Flávio Henrique Baggio Aguiar Sports and Energy Drinks Volume 10 the Science of Beverages, 2019
Influence of an arginine-containing toothpaste on bond strength of different adhesive systems to eroded dentin General Dentistry, 2016
Effect of 7.5% hydrogen peroxide containing remineralizing agents on hardness, color change, roughness and micromorphology of human enamel American Journal of Dentistry, 2015
Effect of Coffee Consumption on Oral Health Flávio Henrique Baggio Aguiar, Núbia Pavesi Pini, Débora Alves Nunes Leite Lima, José Roberto Lovadino Coffee in Health and Disease Prevention, 2015
Effect of light curing protocol on degree of conversion of composites Anderson Catelan, Maria do Carmo Aguiar Jordão Mainardi, Giulliana Panfiglio Soares, Adriano Fonseca de Lima, Gláucia Maria Bovi Ambrosano, Débora Alves Nunes Leite Lima, Giselle Maria Marchi, Flávio Henrique Baggio Aguiar Acta Odontologica Scandinavica, 2014
Influence of coffee and red wine on tooth color during and after bleaching Gabriel Côrtes, Núbia Pavesi Pini, Débora Alves Nunes Leite Lima, Priscila Christiane Susy Liporoni, Egberto Munin, Gláucia Maria Bovi Ambrosano, Flávio Henrique Baggio Aguiar, José Roberto Lovadino Acta Odontologica Scandinavica, 2013
A complete esthetic approach with multiple diastemata in anterior teeth: 1-Year follow-up General Dentistry, 2013
Evaluation of sealing ability of three materials used as barriers over the remaining filling material after post space preparation. Acta Odontologica Latinoamericana AOL, 2013
Effect of curing light distance and different mouthwashes on the sorption and solubility of a nanofilled composite. European Journal of Esthetic Dentistry Official Journal of the European Academy of Esthetic Dentistry, 2013
Can extended photoactivation time of resin-based fissure sealer materials improve ultimate tensile strength and decrease water sorption/solubility? European Journal of Dentistry, 2012
Does an additional UV LED improve the degree of conversion and knoop hardness of light-shade composite resins? European Journal of Dentistry, 2012
Advances in dental veneers: Materials, applications, and techniques Clinical Cosmetic and Investigational Dentistry, 2012
Evaluation of ultrasound and light sources as bleaching catalysts - an in vitro study. European Journal of Esthetic Dentistry Official Journal of the European Academy of Esthetic Dentistry, 2012
Influence of chemical or physical catalysts on high concentration bleaching agents. European Journal of Esthetic Dentistry Official Journal of the European Academy of Esthetic Dentistry, 2011
Influence of the light curing tip distance and material opacity on selected physical properties of a pit and fissure sealant Pediatric Dentistry, 2011
Microhardness of nanofilled composite resin light-cured by LED or QTH units with different times Brazilian Journal of Oral Sciences, 2011
Impact of extended radiant exposure time on polymerization depth of fluoride-containing fissure sealer materials. Acta Odontologica Latinoamericana AOL, 2011
Effect of polymerization modes and resin composite on the temperature rise of human dentin of different thicknesses: An in vitro study Operative Dentistry, 2005
