DNA Self-Assembly on Lithographic Patterns: Fabrication Methods Alexey V. Shnitko, Irina V. Martynenko Small Methods, 2026 The combination of structural DNA nanotechnology and lithographic surface patterning has recently advanced from proof‐of‐principle demonstrations to device‐relevant applications, including field‐effect transistors, prototypical photodiodes, plasmonic metasurfaces, quantum light sources, dynamic nanomachines, and single‐molecule sensor arrays where molecular devices are patterned on the sub‐micrometer scale. Recent advances now allow the deterministic placement of DNA nanostructures of varying geometries, sizes, and complexities onto chip surfaces, as well as subsequent on‐surface assembly into hierarchical architectures. In this review, we summarize fabrication strategies for DNA self‐assembly on lithographically patterned substrates, focusing on two main areas: (i) methods for designing and depositing DNA nanostructures and enabling surface self‐assembly, and (ii) techniques for fabricating patterned surfaces through lithography and chemical functionalization. We then highlight advances across optoelectronics, quantum technologies, and biotechnology, identify key remaining challenges, and conclude with a perspective on how the combination of DNA nanotechnology and lithography may provide a foundation for next‐generation nanoscale devices.
Structural peculiarities of lysozyme-graphene oxide adsorption complexes Vitalii A. Bunyaev, Alexey V. Shnitko, Maria G. Chernysheva, Alexander L. Ksenofontov, Gennadii A. Badun Fullerenes Nanotubes and Carbon Nanostructures, 2022 Lysozyme adsorption complexes with graphene oxide (GO) and reduced GO were studied by means of radiotracer method using tritium as a label. The experiment includes adsorption study, with amount and enzymatic activity control, and revealing structural peculiarities of lysozyme adsorbed on graphene oxide surface by analysis of tritium distribution in the amino acid residues after the bombardment with atomic tritium. Experimental and molecular docking results suggested that lysozyme adsorbs directly on GO by formation of bonds between GO surface and Cys6 and Arg128 that contribute to formation conformation, in which aromatic residues forms π-π bonds with GO surface, and positively charged amino acid residues bind with O-containing groups of GO via electrostatic attraction. In the follow-up layers lysozyme molecules interact with each other that result in changes and adsorbed lysozyme molecules resulting in changes in protein structure. Such changes are not critically significant but lead to increase of availability of an active site of the enzyme for binding with a substrate after the desorption from the multilayer.
Pluronics and Brij-35 Reduce the Bacteriolytic Activity of Lysozyme A. V. Shnitko, M. G. Chernysheva, S. A. Smirnov, P. A. Levashov, G. A. Badun Moscow University Chemistry Bulletin, 2020 The influence of nonionic surfactants on the bacteriolytic activity of lysozyme is studied on the model cells of Micrococcus luteus using Pluronics P123, L121, and F127, as well as Brij-35. These compounds form complexes with lysozyme through hydrogen-bonding interactions between the ethylene oxide fragments of surfactant and the amino acids residues of lysozyme surface. The bacteriolytic activity of lysozyme decreases in the order of F127 < P123 < L121 ≈ Brij-35, probably because of the steric hindrances of the protein’s interaction with the cells’ substrate.
