Anzhelika Lushova

EDUCATION

2021-09-01 - 2023-06-07 | Master of Natural Sciences (Genetics) (Faculty of Biology and Biotechnology)
2017-09-01 - 2021-07-01 | Bachelor of Technics and Technologies on the specialty/educational program Biotechnology (Faculty of Biology and Biotechnology)

RESEARCH, TEACHING, or OTHER INTERESTS

Immunology, Molecular Biology, Cancer Research, Virology

Scopus Publications

Cross-sectional study of camelpox virus in the western and southern regions of Kazakhstan in 2021-2022
Veterinary Integrative Sciences, 2026
Natural and in vitro-generated regulatory T-cell subsets differentially modulate phenotype and drug response in K562 leukemic cells
Yekaterina O. Ostapchuk, Sofiya A. Kan, Anzhelika V. Lushova, Raikhan Tleulieva, Nurshat Abdolla, Aikyn Kali, Yuliya V. Perfilyeva
Cancer Treatment and Research Communications, 2026
and VEGFR1⁺ K562 cells. Neutralization of TGF-β1 in the co-culture system partially attenuated the nTreg-associated upregulation of PDGFRβ and ABCG2. iTregs increased ABCG2 expression, which was also reduced upon TGF-β1 blockade. Tr1 cells increased the proportion of CD24⁺ cells, upregulated PDGFRβ and ABCG2, and increased K562 cell metabolic activity/viability under drug-free conditions. All Treg subsets attenuated daunorubicin cytotoxicity, whereas reduced sensitivity to gemcitabine was observed only in iTreg- and Tr1-containing cultures. These findings suggest that Treg cell subsets may directly promote stemness-associated and drug-tolerance-related features in K562 cells in vitro, supporting a tumor-promoting role within the leukemia microenvironment and warranting further validation in primary leukemia samples and in vivo models.
Occurrence and epidemiology of bovine coronavirus in cattle in Kazakhstan
Yuliya V. Perfilyeva, Andrey V. Zhigailov, Angelina A. Malysheva, Alena S. Cherusheva, Karina R. Ivanova, Zhanna A. Berdygulova, Akerke O. Bissenbay, Saltanat A. Kuatbekova, Zhaniya M. Dosmagambet, Anzhelika V. Lushova, Sofiya A. Kan, Artyom V. Kuligin, Moldir M. Kuatbek, Akzhigit S. Mashzhan, Nurshat Abdolla, Dinara A. Naizabayeva, Anna S. Nizkorodova, Perizat B. Akshalova, Kirpichenko V. Vladimir, Aida M. Abdybekova, Aralbek S. Rsaliyev, Yergali O. Abduraimov, Seidigapbar M. Mamadaliyev, Yuriy A. Skiba, Yekaterina O. Ostapchuk
Veterinary Journal, 2025
Effect of calcitriol on myeloid-derived suppressor cells in physiological aging
Yuliya V. Perfilyeva, Aikyn Kali, Diana S. Aben, Yulduz R. Abdusattarova, Anzhelika V. Lushova, Yekaterina O. Ostapchuk, Raikhan Tleulieva, Anastassiya V. Perfilyeva, Kamalidin O. Sharipov, Timur I. Davlyatshin, Nurshat Abdolla
Journal of Steroid Biochemistry and Molecular Biology, 2025
Survey and Associated Risk Factors for the Presence of Ruminant Pestiviruses in Domestic Ovine and Caprine Populations from Kazakhstan
Andrey V. Zhigailov, Yuliya V. Perfilyeva, Angelina A. Malysheva, Alena S. Cherusheva, Zhanna A. Berdygulova, Dinara A. Naizabayeva, Karina R. Ivanova, Saltanat A. Kuatbekova, Zhaniya M. Dosmagambet, Anzhelika V. Lushova, Sofiya A. Kan, Artyom V. Kuligin, Akerke O. Bissenbay, Moldir M. Kuatbek, Akzhigit S. Mashzhan, Nurshat Abdolla, Anna S. Nizkorodova, Elina R. Maltseva, Aralbek S. Rsaliyev, Yergali O. Abduraimov, Ainur A. Zhaksylykova, Aida M. Abdybekova, Seidigapbar M. Mamadaliyev, Yuriy A. Skiba, Yekaterina O. Ostapchuk
Viruses, 2025
Pestiviruses, particularly bovine viral diarrhea virus (BVDV), cause significant economic losses worldwide. While cattle are the primary hosts for BVDV, sheep and goats can also be affected. This nationwide survey aimed to assess the prevalence, genetic characteristics, and risk factors associated with pestiviruses in sheep and goats in Kazakhstan. A one-off cross-sectional study was conducted to estimate the prevalence of pestiviruses in sheep and goats across 58 districts in 17 oblasts of Kazakhstan. A total of 2028 animals were examined using antibody ELISA, and RT-qPCR was performed on 2056 samples. Logistic regression models were used to identify potential risk factors linked to pestiviral infection. The overall prevalence of pestiviral infection in small ruminants was estimated to be 53.7% by ELISA and 2.5% by RT-qPCR. Regression analysis revealed that age, farm type, and geographic location were risk factors for pestiviral infections in small ruminants in Kazakhstan. Partial sequence analysis of the 5′-untranslated region confirmed the presence of BVDV2. Phylogenetic analysis revealed two distinct clusters of Kazakhstani BVDV2 strains, which were significantly different from known BVDV2 genotypes. No other ruminant pestiviruses were identified. The results highlight the importance of integrating small ruminants into BVDV infection control strategies to mitigate risks to livestock.
Survey of tick-borne relapsing fever borreliae in southern and southeastern Kazakhstan
Yekaterina O. Ostapchuk, Akerke O. Bissenbay, Artyom V. Kuligin, Andrey V. Zhigailov, Yuliya V. Perfilyeva, Sofiya A. Kan, Anzhelika V. Lushova, Olga A. Stukolova, Zaure Z. Sayakova, Nurshat Abdolla, Andrey M. Dmitrovskiy, Akzhigit S. Mashzhan, Saltanat A. Kuatbekova, Zhaniya Dosmagambet, Zhanna Zh. Shapiyeva, Dinara A. Naizabayeva, Nailya K. Ospanbekova, Aidyn Yeszhanov, Ilyas A. Akhmetollayev, Yuriy A. Skiba
Ticks and Tick Borne Diseases, 2024
Tick-borne relapsing fever group borreliae (TBRFGB) are spirochetes that cause disease in humans and animals. Little is known about the prevalence of TBRFGB infections in ticks and humans in Kazakhstan. A total of 846 ticks belonging to ten species of the family Ixodidae and three species of the family Argasidae were collected from the vegetation, poultry shelters, domestic ruminants, bitten humans, pigeons, dogs and house walls in four oblasts of the southern and southeastern regions of Kazakhstan. The ticks were subjected to DNA extraction and identification of TBRFGB by conventional PCR using primers targeting flagella subunit B (flaB), glycerophosphodiester phosphodiesterase (glpQ) and P66 porin (P66) genes. The overall infection rate of TBRFGB in the ticks was 6.2 % (46/846). TBRFGB DNA was identified in Ixodes persulcatus (5.5 %; 26/477), Ornithodoros tartakovskyi (6 %; 2/36) and Argas persicus (13.4 %; 18/134) ticks. Partial sequencing of flaB, glpQ and P66 genes identified Borrelia miyamotoi in I. persulcatus and Borrelia anserina in A. persicus. To detect the presence of B. miyamotoi infection in people in the study region, we performed serological analysis of samples collected from 42 patients admitted to hospital with fever of unknown etiology or with a history of a tick bite. The analysis revealed IgM and IgG antibodies against one or several B. miyamotoi antigens in 10 % and 5 % of patients, respectively. The data obtained provide strong evidence of the presence of B. miyamotoi and B. anserina in the southern and southeastern regions of Kazakhstan, underscoring the need for increased awareness of potential infections caused by these borreliae in these regions.
Long-term changes in the phenotype and cytokine production of monocytes in COVID-19 recovered and vaccinated individuals
Yekaterina O. Ostapchuk, Anzhelika V. Lushova, Sofia A. Kan, Nurshat Abdolla, Aikyn Kali, Raikhan Tleulieva, Anastassiya V. Perfilyeva, Yuliya V. Perfilyeva
Infection and Immunity, 2024
Monocytes play a crucial role in the immune response against pathogens. Here, we sought to determine COVID-19 and the vaccine Gam-COVID-Vac induce long-term changes in the phenotype and cytokine production of circulating monocytes. Monocytes were purified from peripheral blood mononuclear cells of healthy donors who had not had COVID-19 or vaccination, who had received two doses of Gam-COVID-Vac, and who had mild/moderate COVID-19 in the last 6 months and evaluated by flow cytometry. To investigate the effect of SARS-CoV-2 proteins, monocytes were cultured for 2 days with or without stimulation with recombinant SARS-CoV-2 S1 and N peptides. Monocytes obtained from vaccinated and recovered individuals showed increased basal expression of HLA-DR, CD63, CXCR2, and TLR7. We also observed an increased frequency of CD63 + classical monocytes in both groups, as well as an increased frequency of HLA-DR + non-classical monocytes in the COVID-19-recovered group compared to the control group. Monocytes from vaccinated and recovered donors produced higher basal levels of IL-6, IL-1β, and TNF-α cytokines. Ex vivo stimulation with SARS-CoV-2 antigens induced increased expression of HLA-DR and TLR7 on monocytes obtained from the control group. The challenge with SARS-CoV-2 antigens had no effect on the production of IL-6, IL-1β, and TNF-α cytokines by monocytes. The acquired data offer compelling evidence of enduring alterations in both the phenotype and functional status of circulating monocytes subsequent to vaccination with Gam-COVID-Vac and mild/moderate COVID-19 infection. At least some of these changes appear to be a consequence of exposure to SARS-CoV-2 S1 and N antigens.
Molecular and serological survey of bovine viral diarrhea virus infection in cattle in Kazakhstan
Andrey V. Zhigailov, Yuliya V. Perfilyeva, Yekaterina O. Ostapchuk, Sofiya A. Kan, Anzhelika V. Lushova, Artyom V. Kuligin, Karina R. Ivanova, Saltanat A. Kuatbekova, Nurshat Abdolla, Dinara A. Naizabayeva, Elina R. Maltseva, Zhanna A. Berdygulova, Akzhigit S. Mashzhan, Yuliya A. Zima, Anna S. Nizkorodova, Yuriy A. Skiba, Seidigapbar M. Mamadaliyev
Research in Veterinary Science, 2023