Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity Anil S. Prajapati, Vishakha A. Pawar, Ketankumar J. Panchal, Ankit P. Sudhir, Bhaumik R. Dave, Darshan H. Patel, R. B. Subramanian BMC Biotechnology, 2018 The site-specific single mutation, led to alteration in substrate specificity from xylan to CMC while in case of double mutant the substrate specificity was altered from xylan to CMC, FP and avicel, indicating the role of aromatic residues on substrate binding, catalytic process and overall catalytic efficiency.
Purification and properties of an endoglucanase from Thermoascus aurantiacus Bhaumik R. Dave, Ankit P. Sudhir, R.B. Subramanian Biotechnology Reports, 2015 An Endo-cellulase was purified to homogeneity using ammonium sulfate precipitation, ion exchange and size exclusion chromatography from newly isolated strain of Thermoascus aurantiacus RBB-1. The recovery and purification fold were 13.3% and 6.6, respectively, after size exclusion chromatography. The purified cellulase has a molecular mass (M) of 35 kDa. Optimum temperature for the enzyme was found to be 70 °C and stability was upto 80 °C for 1 h. Along with higher stability at 80 °C, enzyme showed half lives of 192 h and 144 h at 50 and 70 °C respectively. The purified cellulase was optimally active at pH 4.0 and was stable over a broad pH range of 3.0–7.0. The enzyme purified showed apparent Km and Vmax values of 37 mg/ml and 82.6 U/min/mg protein respectively with higher salt tolerance of 10% for 1 h.
Production and amplification of an L-asparaginase gene from actinomycete isolate Streptomyces ABR2 Ankit P. Sudhir, Bhaumik R. Dave, Kalkal A. Trivedi, Ramalingam B. Subramanian Annals of Microbiology, 2012 l-Asparaginase is used as a therapeutic molecule for the treatment of acute lymphoblastic leukaemia (ALL). It is also used in the food industry to remove acrylamide from fried starch-based foods. In an attempt to identify novel organisms as sources of this enzyme, 32 different actinomycetes isolates were obtained from soil samples near Vallabh Vidyanagar, India. From these 32 isolates, 11 l-asparaginase-positive isolates were obtained with a rapid plate assay method. The maximum l-asparaginase producing strain was identified as Streptomyces sp. by 16S r-RNA sequencing. The effect of inoculum size, pH and l-asparagine concentration on l-asparaginase production was also checked. An increase in l-asparaginase activity from 12.5 IU/mL to 18.8 IU/mL was found. The gene coding for l-asparaginase (~800 bp) was isolated from Streptomyces sp. ABR2.
Engineering the plant microbiome: synthetic community approaches to enhance crop protection K Panchal, A Sudhir, AS Prajapati Frontiers in Plant Science 16, 1705289 , 2026 2026 Citations: 8
Antibacterial Activity of Silver-Modified CuO Nanoparticle-Coated Masks T Udawant, P Thorat, P Thapa, M Patel, S Shekhawat, R Patel, A Sudhir, ... Bioengineering 11 (12), 1234 , 2024 2024 Citations: 5
Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity AS Prajapati, VA Pawar, KJ Panchal, AP Sudhir, BR Dave, DH Patel, ... BMC biotechnology 18 (1), 9 , 2018 2018 Citations: 18
Enhanced catalysis of L-asparaginase from Bacillus licheniformis by a rational redesign AP Sudhir, VV Agarwaal, BR Dave, DH Patel, RB Subramanian Enzyme and microbial technology 86, 1-6 , 2016 2016 Citations: 42
Designing Improved Enzymes of industrial application from marine microorganisms using Protein Engineering AS Prajapati, K Panchal, RB Subramanian, DH Patel, AP Sudhir, ... AGU Fall Meeting Abstracts 2015, OS13B-2047 , 2015 2015
Purification and properties of an endoglucanase from Thermoascus aurantiacus BR Dave, AP Sudhir, RB Subramanian Biotechnology reports 6, 85-90 , 2015 2015 Citations: 47
Purification and properties of an endoglucanase from Thermoascus aurantiacus. Biotechnol Reports 6: 85–90 BR Dave, AP Sudhir, RB Subramanian 2015 Citations: 6
Optimization of process parameters for cellulase production by Bacillus licheniformis MTCC 429 using RSM and molecular characterization of cellulase gene BR Dave, P Parmar, A Sudhir, K Panchal, RB Subramanian Journal of Bioprocessing & Biotechniques 5 (03), 212 , 2015 2015 Citations: 21
Characterization of a Recombinant Glutaminase-Free l -Asparaginase (ansA3) Enzyme with High Catalytic Activity from Bacillus licheniformis AP Sudhir, BR Dave, AS Prajapati, K Panchal, D Patel, RB Subramanian Applied biochemistry and biotechnology 174 (7), 2504-2515 , 2014 2014 Citations: 38
Cloning, expression, characterization and genetic modification of L-asparaginase from Bacillus licheniformis AP SUDHIR 2014
Zymographic identification and biochemical characterization of chitinase against phytofungal pathogens U Pandya, A Sudhir, H Gohel, RB Subramanian, M Saraf Journal of Microbiology, Biotechnology and Food Sciences 9 (4), 44-47 , 2014 2014 Citations: 5
Cellulases production under solid state fermentation using agro waste as a substrate and its application in saccharification by Trametes hirsuta NCIM BR Dave, P Parmar, A Sudhir, N Singal, RB Subramanian Journal of Microbiology, Biotechnology and Food Sciences 2014, 203-208 , 2014 2014 Citations: 9
Modified and simple method for isolation of genomic DNA from fungal culture P Parmar, B Dave, A Sudhir, K Panchal, RB Subramanian African journal of microbiology research 8 (22), 2261-2263 , 2014 2014 Citations: 2
Enhancement of cellulase activity by a new strain of Thermoascus aurantiacus: optimisation by statistical design response surface methodology BR Dave, AP Sudhir, P Parmar, S Pathak, DP Raykundaliya, ... Biocatalysis and Agricultural Biotechnology 2 (2), 108-115 , 2013 2013 Citations: 30
Physiological, biochemical and molecular response of plants against heavy metals stress P Parmar, B Dave, A Sudhir, K Panchal, RB Subramanian International Journal of Current Research 5 (1), 80-89 , 2013 2013 Citations: 55
Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum P Parmar, A Sudhir, R Preethi, B Dave, K Panchal, RB Subramanian, ... Scientific Research Publishing , 2013 2013 Citations: 26
Production and amplification of an l -asparaginase gene from actinomycete isolate Streptomyces ABR2 AP Sudhir, BR Dave, KA Trivedi, RB Subramanian Annals of microbiology 62 (4), 1609-1614 , 2012 2012 Citations: 33
Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation BR Dave, AP Sudhir, M Pansuriya, DP Raykundaliya, RB Subramanian Bioprocess and biosystems engineering 35 (8), 1343-1353 , 2012 2012 Citations: 27
MOST CITED SCHOLAR PUBLICATIONS
Physiological, biochemical and molecular response of plants against heavy metals stress P Parmar, B Dave, A Sudhir, K Panchal, RB Subramanian International Journal of Current Research 5 (1), 80-89 , 2013 2013 Citations: 55
Purification and properties of an endoglucanase from Thermoascus aurantiacus BR Dave, AP Sudhir, RB Subramanian Biotechnology reports 6, 85-90 , 2015 2015 Citations: 47
Enhanced catalysis of L-asparaginase from Bacillus licheniformis by a rational redesign AP Sudhir, VV Agarwaal, BR Dave, DH Patel, RB Subramanian Enzyme and microbial technology 86, 1-6 , 2016 2016 Citations: 42
Characterization of a Recombinant Glutaminase-Free l -Asparaginase (ansA3) Enzyme with High Catalytic Activity from Bacillus licheniformis AP Sudhir, BR Dave, AS Prajapati, K Panchal, D Patel, RB Subramanian Applied biochemistry and biotechnology 174 (7), 2504-2515 , 2014 2014 Citations: 38
Production and amplification of an l -asparaginase gene from actinomycete isolate Streptomyces ABR2 AP Sudhir, BR Dave, KA Trivedi, RB Subramanian Annals of microbiology 62 (4), 1609-1614 , 2012 2012 Citations: 33
Enhancement of cellulase activity by a new strain of Thermoascus aurantiacus: optimisation by statistical design response surface methodology BR Dave, AP Sudhir, P Parmar, S Pathak, DP Raykundaliya, ... Biocatalysis and Agricultural Biotechnology 2 (2), 108-115 , 2013 2013 Citations: 30
Utilization of Jatropha deoiled seed cake for production of cellulases under solid-state fermentation BR Dave, AP Sudhir, M Pansuriya, DP Raykundaliya, RB Subramanian Bioprocess and biosystems engineering 35 (8), 1343-1353 , 2012 2012 Citations: 27
Identification of a SSR marker (TOM-144) linked to Fusarium wilt resistance in Solanum lycopersicum P Parmar, A Sudhir, R Preethi, B Dave, K Panchal, RB Subramanian, ... Scientific Research Publishing , 2013 2013 Citations: 26
Optimization of process parameters for cellulase production by Bacillus licheniformis MTCC 429 using RSM and molecular characterization of cellulase gene BR Dave, P Parmar, A Sudhir, K Panchal, RB Subramanian Journal of Bioprocessing & Biotechniques 5 (03), 212 , 2015 2015 Citations: 21
Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity AS Prajapati, VA Pawar, KJ Panchal, AP Sudhir, BR Dave, DH Patel, ... BMC biotechnology 18 (1), 9 , 2018 2018 Citations: 18
Cellulases production under solid state fermentation using agro waste as a substrate and its application in saccharification by Trametes hirsuta NCIM BR Dave, P Parmar, A Sudhir, N Singal, RB Subramanian Journal of Microbiology, Biotechnology and Food Sciences 2014, 203-208 , 2014 2014 Citations: 9
Engineering the plant microbiome: synthetic community approaches to enhance crop protection K Panchal, A Sudhir, AS Prajapati Frontiers in Plant Science 16, 1705289 , 2026 2026 Citations: 8
Purification and properties of an endoglucanase from Thermoascus aurantiacus. Biotechnol Reports 6: 85–90 BR Dave, AP Sudhir, RB Subramanian 2015 Citations: 6
Antibacterial Activity of Silver-Modified CuO Nanoparticle-Coated Masks T Udawant, P Thorat, P Thapa, M Patel, S Shekhawat, R Patel, A Sudhir, ... Bioengineering 11 (12), 1234 , 2024 2024 Citations: 5
Zymographic identification and biochemical characterization of chitinase against phytofungal pathogens U Pandya, A Sudhir, H Gohel, RB Subramanian, M Saraf Journal of Microbiology, Biotechnology and Food Sciences 9 (4), 44-47 , 2014 2014 Citations: 5
Modified and simple method for isolation of genomic DNA from fungal culture P Parmar, B Dave, A Sudhir, K Panchal, RB Subramanian African journal of microbiology research 8 (22), 2261-2263 , 2014 2014 Citations: 2
Designing Improved Enzymes of industrial application from marine microorganisms using Protein Engineering AS Prajapati, K Panchal, RB Subramanian, DH Patel, AP Sudhir, ... AGU Fall Meeting Abstracts 2015, OS13B-2047 , 2015 2015
Cloning, expression, characterization and genetic modification of L-asparaginase from Bacillus licheniformis AP SUDHIR 2014
