Microbial-Derived Anti-Cancer Compounds: Advances in Drug Discovery, Bioengineering, and Therapeutic Applications Ekta Tyagi, Divya Jain, Rajabrata Bhuyan, Anand Prakash Anti Cancer Agents in Medicinal Chemistry, 2026 Introduction: Microbial metabolites represent a valuable source of bioactive compounds with promising anticancer properties. However, conventional drug discovery approaches are time-intensive and resource-demanding. Methods: Recent developments in artificial intelligence (AI), machine learning (ML), molecular docking, and quantitative structure-activity relationship (QSAR) modeling have been examined for their role in the identification and optimization of microbial metabolites. Results: AI-driven approaches have significantly enhanced compound screening and prediction of therapeutic efficacy. Nanocarrier-based drug delivery systems have improved the bioavailability, specificity, and stability of microbial metabolites while minimizing systemic toxicity. Despite these advancements, challenges remain in clinical translation due to the lack of in vivo validation and comprehensive pharmacokinetic data. Discussion: This review highlights the integration of advanced computational tools and nanotechnology in accelerating the discovery and delivery of microbial-derived anticancer agents. Conclusion: Future directions should focus on integrating AI with synthetic biology to engineer microbial strains capable of producing enhanced bioactive compounds. Additionally, leveraging nanotechnology could refine targeted delivery mechanisms. A deeper understanding of molecular pathways and drug resistance mechanisms is essential to support the development of combination therapies. Overall, microbialderived compounds hold substantial potential in advancing precision oncology.
Next-Gen Biofilm Control: Gene Editing and Computational Approaches Ekta Tyagi, Anjali Sachan, Rajabrata Bhuyan, Prema Kumari, Anand Prakash APMIS, 2025 Biofilms are microbial communities enclosed in an extracellular polymeric substance (EPS), significantly contributing to antimicrobial resistance (AMR) in medical, industrial, and environmental settings. Their matrix enhances microbial survival, inhibits antibiotic penetration, and facilitates horizontal gene transfer, worsening the AMR crisis. Conventional antimicrobial treatments often fail against biofilms, necessitating novel therapeutic strategies. Emerging biofilm‐targeted interventions, such as nanotechnology‐based antimicrobials, bacteriophage therapy, and CRISPR‐Cas9 gene editing, offer promising solutions. Nanoparticles improve drug delivery, bacteriophages selectively lyse resistant bacterial populations, and CRISPR‐Cas9 disrupts AMR‐related genes and biofilm virulence factors. Additionally, AI and ML are advancing biofilm prediction models and antimicrobial optimization, paving the way for precision‐targeted interventions. This review explores biofilm biology and next‐generation biofilm control strategies, with a focus on AI‐driven bioinformatics. Future research should focus on clinical translation, regulatory standardization, and scalable implementation in healthcare and industrial settings to combat biofilm‐associated AMR.
Computational and In Vitro Evaluation of Plumbagin and Griseofulvin as Natural PIM1 Kinase Inhibitors for Potential Anticancer Therapy Ekta Tyagi, Akanksha Kotiya, Anand Prakash, Rajabrata Bhuyan Chemistryselect, 2025 The rising global incidence of cancer, coupled with the limitations of current treatments, underscores the need for novel therapeutic agents. This study investigates the anticancer potential of natural compounds, focusing on identifying and characterizing underexplored molecules with promising pharmacological properties. QSAR modeling identified plumbagin and griseofulvin as lead compounds with potent cytotoxicity across various cancer cell lines, including breast, colon, liver, lung, and leukemia. In vitro assays revealed dose‐dependent cytotoxic effects, with plumbagin showing IC50 values of 4.13 (HepG2) and 8.74 µM (A549), while griseofulvin exhibited IC50 values of 44.76 and 26.3 µM, respectively. ADME and toxicity profiling confirmed their drug‐likeness, compliance with Lipinski's rule, and high oral bioavailability (>96%). Annexin V assays further supported their anticancer potential. Target prediction identified PIM1 kinase as a common key molecular target. Molecular docking and dynamics simulations revealed stable, thermodynamically favorable binding, with binding free energies of −14.71 kcal/mol (plumbagin) and −19.58 kcal/mol (griseofulvin). These findings suggest both compounds are promising PIM1 inhibitors, capable of modulating oncogenic pathways. This study underscores the therapeutic potential of natural compounds in targeted anticancer drug development. Further in vivo studies are essential to confirm the efficacy and safety of these compounds.
Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb Swati Chaudhary, Rajabrata Bhuyan, Divya Jain, Anand Prakash Recent Patents on Biotechnology, 2025 Introduction: Medicines and herbal formulations are derived from different parts of medicinal plants, which are the best-known sources for treating various diseases. This research focuses on assessing the antimicrobial potential of crude extracts from the leaves and roots of Cenchrus biflorus Roxb. Methods: Methanol, hydroethanol (50:50), and aqueous extracts were obtained using the Soxhlet extraction method. The disc diffusion method was used to study the antimicrobial activity of the extracts against a variety of test microorganisms, including bacteria (Escherichia coli and Bacillus subtilis) and fungus (Aspergillus niger). The disc diffusion method was used to assess bacterial susceptibility, revealing the potent inhibitory effect of the methanol extract on E. coli. All extracts demonstrated significant antimicrobial activity against various microorganisms. Results: Remarkably, methanol extract of leaf demonstrated the highest antibacterial activity, with a 16.3 ± 1.78 mm zone of inhibition (ZOI) with Activity Index (AI) of 0.875, and a Relative Percentage Inhibition (RPI) of 80 against E. coli, followed by Bacillus subtilis (ZOI = 15.5 ± 1.31 mm, AI = 0.869, RPI = 78.57). The methanol extract of the root showed strong antifungal activity against Aspergillus niger (with a 12.9 ±1 mm ZOI, AI = 0.636, and RPI = 42.85), while the water extract of the root displayed 7.8 mm inhibition zones. Discussion: Methanol and hydroethanol extracts of the leaf and root exhibited strong inhibitory effects against selected microbial strains. Each plant solvent extract suppressed microbial development in a distinct manner, and methanol and hydroethanol extracts inhibited microbial development more efficiently than aqueous extracts. Interestingly, water extracts had the least effective inhibitory effects across all strains. Notably, water extracts showed the weakest inhibitory effects against all strains. Conclusion: The current study demonstrated the efficacy of crude extracts of Cenchrus biflorus Roxb. against the tested strains of bacteria and fungi and also discussed their potential application as antibacterial agents for combating infectious diseases.
Pharmacognostical Analysis, Quantitative Determination, and in-vitro Antioxidant Activity of Chloris virgata Sw. Whole Plant Letters in Applied Nanobioscience, 2024 An Annual grass Chloris virgata Sw. has been utilized by tribal societies to treat inflammation, rheumatism, and other human ailments; as far evidence is not available regarding the purity, safety, and efficacy of this plant. This is the first study to determine the pharmacognostic and phytochemical standards along with its antioxidant activity by standardized protocols. Organoleptic analysis revealed the presence of different characteristics in the intact parts of C. virgata. Cork cells, lignified cells, xylem cells, calcium oxalate crystals, starch granules, trichomes, stone cells, and spiral xylem vessels were observed from the powder analysis of C. virgata. Physicochemical analysis determined the values of total ash content (10%), acid-insoluble ash content (4.8%), water-soluble ash content (5%), sulfated ash content (7.1%), moisture content (10.19%), and foreign organic matter (3.2%) from the powder of whole plant extract of C. virgata. The green-colored methanol extract has been determined to have a higher extractive yield (0.159%) than the other extracts. Fluorescence analysis revealed different colors, such as grey, brown, light green, etc., under different chemical treatments. Qualitative analysis determined the presence of high phenols, flavonoids, and saponins, whereas other phytochemicals were present in moderate and low amounts in different extracts of C. virgata. Results of the quantitative analysis showed that the methanolic plant extract had the highest total flavonoid (37.0 ± 0.027mg/g), phenolic (30.2 ± 0.034mg/g), and tannin content (28.1 ± 0.007mg/g). Antioxidant properties determined by DPPH and metal chelation assays determined that the methanol extract acts as an effective inhibitor and chelator of metal ions with IC50 values of 53.66 ± 0.001µg/ml and 96.79 ± 0.003µg/ml. The highest ferric-reducing antioxidant activity was revealed in hydroethanolic extract (245.6 ± 0.003mg/g), followed by other extracts. Thus, it can be concluded that the above study results would serve as a basis for establishing the standards of pharmacopeia and in the authentication of medicinally important plant C. virgata.
Sustainable biofuel production from agro-waste: Technological status and future prospects Agricultural Waste Uses Recycling and Management, 2024
Role of plants in nanoparticle synthesis Tanya Kapoor, Md Azizur Rahman, Shally Pandit, Anand Prakash Nanomaterials in Clinical Therapeutics Synthesis and Applications, 2022
Next‐Gen Biofilm Control: Gene Editing and Computational Approaches PA Tyagi E, Sachan A, Bhuyan R, Kumari P Journal of Pathology, Microbiology and Immunology - the APMIS journal 133 … , 2025 2025
Computational and In Vitro Evaluation of Plumbagin and Griseofulvin as Natural PIM1 Kinase Inhibitors for Potential Anticancer Therapy. BR Tyagi E, Kotiya A, Prakash A Chemistry Select 10 (36), e02792. , 2025 2025 Citations: 2
Antimicrobial Activity of Leaf and Root Parts of Cenchrus biflorus Roxb AP S Chaudhary, R Bhuyan, D Jain Recent Patents on Biotechnology , 2025 2025
Microbial-Derived Anti-Cancer Compounds: Advances in Drug Discovery, Bioengineering, and Therapeutic Applications. 2025. PA Tyagi E, Jain D, Bhuyan R Anti-Cancer Agents in Medicinal Chemistry. , 2025 2025
Enhancing Multi-Targeted Cancer Therapy with AI and Computational Drug Design APRB Ekta Tyagi Cancer Therapy & Oncology International journal 28 (2), 001-009 , 2025 2025
COMPARATIVE PHARMACOGNOSTICAL EVALUATION, DRUG STANDARDIZATION AND QUALITY ASSURANCE FROM DIFFERENT PARTS OF TRIBULUS TERRESTRIS L. AP Pracheta Janmedaa, Divya Kumaria, Mukesh Meenab Reviews In Food And Agriculture (RFNA) 5 (1), 79-94 , 2025 2025
Revolutionizing Anti-Cancer Drug Discovery: The Role of Artificial Intelligence RB Ekta Tyagi, Prema Kumari, Anand Prakash Int J Bioinfor Intell Comput 4 (1), 1-38 , 2025 2025 Citations: 14
Life cycle assessment and techno-economic analysis of nanotechnology-based wastewater treatment: Status, challenges and future prospectives S Pandit, N Yadav, P Sharma, A Prakash, A Kuila Journal of the Taiwan Institute of Chemical Engineers 166, 105567 , 2025 2025 Citations: 38
Concomitant inhibitor-tolerant cellulase and xylanase production towards sustainable bioethanol production by Zasmidiumcellare CBS 146.36 S Pant, A Prakash, PR Vundavilli, KC Khadanga, A Kuila, TM Aminabhavi, ... Fuel 375, 132593 , 2024 2024 Citations: 14
Antimicrobial peptides: a novel and natural approach as antibiofouling mediator H Sharma, V Dave, E Tyagi, A Prakash Biologia 79 (8), 2515-2533 , 2024 2024 Citations: 4
A comprehensive review on technical lignin, lignin hydrogels, properties, preparation, applications & challenges in lab to market transition S Pandit, P Sharma, A Prakash, B Lal, R Bhuyan, I Ahmad, A Kuila Industrial Crops and Products 211, 118262 , 2024 2024 Citations: 40
Nonthermal Food Processing, Safety, and Preservation A Prakash, A Kuila John Wiley & Sons , 2024 2024 Citations: 3
Bio-Aviation Fuel via Catalytic Hydrocracking of Waste Cooking Oil S Pandit, A Prakash, A Kuila Biojet Fuel: Current Technology and Future Prospect, 147-162 , 2024 2024 Citations: 2
Sustainable Biofuel Production from Agro-Waste: Technological Status and Future Prospects KSASAPA Kuila Agricultural Waste Uses, Recycling and Management , 2024 2024
Improvised strategy of ethanolic nanovesicular gel of phospholipon 90G for transdermal delivery of luliconazole to mitigate fungal diseases V Dave, N Gupta, A Prakesh, P Sharma Biomass Conversion and Biorefinery 13 (17), 15463-15469 , 2023 2023
Pharmacognostical, physicochemica studied S Chaudhary, D Kumari, P Yadav, A Prakash Research Journal of Recent Sciences ______ 12 (3), 1-12 , 2023 2023 Citations: 2
Green synthesized cobalt nanoparticles from Trianthema portulacastrum L. as a novel antimicrobials and antioxidants PRGH Anand Prakash, Srija Sur, Vivek Dave, Prashansa Sharma, Suvadra Das PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 15 , 2023 2023 Citations: 22
Ecofriendly fabrication of cobalt nanoparticles using Azadirachta indica (neem) for effective inhibition of Candida‑like fungal infection in medicated nano‑coated textile AK Devsuni Singh, Prashansa Sharma, Suman Pant, Vivek Dave, Rekha Sharma ... Environmental Science and Pollution Research, 16 , 2023 2023 Citations: 7
Production and applications of artificial seeds: a review D Ravi, P Anand Int Res J Biol Sci 1 (5), 74-78 , 2012 2012 Citations: 72
Isolation and Screening of Extracellular Protease Enzyme from Bacterial and Fungal Isolates of Soil S Arun, Kumar, S Vinay, S Jyoti, Y Bindu, A Afroz, P Anand International Journal of Scientific Research in Environmental Sciences 3 (9 … , 2015 2015 Citations: 59
Development and standardization of quality control parameters of different parts of Trianthema portulacastrum L. P Anand, J Pracheta, P Purnima, B Sneha, S Vinay SN Applied Sciences 1 (1108), https://doi.org/10.1007/s42452-019-1074- , 2019 2019 Citations: 46
A comprehensive review on technical lignin, lignin hydrogels, properties, preparation, applications & challenges in lab to market transition S Pandit, P Sharma, A Prakash, B Lal, R Bhuyan, I Ahmad, A Kuila Industrial Crops and Products 211, 118262 , 2024 2024 Citations: 40
Life cycle assessment and techno-economic analysis of nanotechnology-based wastewater treatment: Status, challenges and future prospectives S Pandit, N Yadav, P Sharma, A Prakash, A Kuila Journal of the Taiwan Institute of Chemical Engineers 166, 105567 , 2025 2025 Citations: 38
Integrated production of ethanol and xylitol from Brassica juncea using Candida sojae JCM 1644 AK Shailja Pant, Ritika, Anand Prakash Bioresource Technology , 2022 2022 Citations: 28
OPTIMIZATION OF PROTEASE PRODUCTION FROM BACTERIA ISOLATED FROM SOIL S Arun, Kumar, S Vinay, S Jyoti, Y Bindu, A Afroz, P Anand APPLIED RESEARCH JOURNAL 1 (7), 388-394 , 2015 2015 Citations: 28
Twin arginine translocase pathway and fast-folding lipoprotein biosynthesis in E. coli : interesting implications and applications H Shruthi, P Anand, V Murugan, K Sankaran Molecular BioSystems 6 (6), 999-1007 , 2010 2010 Citations: 27
Green synthesized cobalt nanoparticles from Trianthema portulacastrum L. as a novel antimicrobials and antioxidants PRGH Anand Prakash, Srija Sur, Vivek Dave, Prashansa Sharma, Suvadra Das PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 15 , 2023 2023 Citations: 22
Pharmacognostical analysis of different parts of Cyperus rotundus L. P Anand, J Divya, T Rashmi, Pracheta Plant Science Today 6 (1), 607-612 , 2019 2019 Citations: 20
A Review on Fermentative Production of Biobutanol From Biomass P Anand, D Ravi, S Vinay Current Biochemical Engineering 3 (1), 37-46 , 2016 2016 Citations: 17
Revolutionizing Anti-Cancer Drug Discovery: The Role of Artificial Intelligence RB Ekta Tyagi, Prema Kumari, Anand Prakash Int J Bioinfor Intell Comput 4 (1), 1-38 , 2025 2025 Citations: 14
Concomitant inhibitor-tolerant cellulase and xylanase production towards sustainable bioethanol production by Zasmidiumcellare CBS 146.36 S Pant, A Prakash, PR Vundavilli, KC Khadanga, A Kuila, TM Aminabhavi, ... Fuel 375, 132593 , 2024 2024 Citations: 14
Expansion and scale up of technology for ethanol production based on the concept of biorefinery S Sur, V Dave, A Prakesh, P Sharma Journal of Food Process Engineering 44 (2), e13582 , 2021 2021 Citations: 11
Effect of Culture Conditions on Protease Production and Activity of Protease from Soil Borne Fungi S Arun, Kumar, S Vinay, S Jyoti, Y Bindu, A Afroz, P Anand International Journal of Scientific Research in Environmental Sciences 3 (11 … , 2015 2015 Citations: 8
Ecofriendly fabrication of cobalt nanoparticles using Azadirachta indica (neem) for effective inhibition of Candida‑like fungal infection in medicated nano‑coated textile AK Devsuni Singh, Prashansa Sharma, Suman Pant, Vivek Dave, Rekha Sharma ... Environmental Science and Pollution Research, 16 , 2023 2023 Citations: 7
Vivid techniques of pretreatment showing promising results in biofuel production and food processing A Prakesh, V Dave, S Sur, P Sharma Journal of Food Process Engineering 44 (2), e13580 , 2021 2021 Citations: 7
Bioactivity and pharmacological potential of Trianthema portulacastrum L. (Angiosperms: Aizoaceae): An overview P Anand, Pracheta, S Vinay Plant Science Today 6 (1), 590-599 , 2019 2019 Citations: 7
Lipase production from mutagenic strain of Fusarium Incarnatum KU377454 and its immobilization using Au@Ag core shells nanoparticles for application in waste cooking oil … J Ritika, S Rekha, B Rupam, P Anand, K Arindam 3 Biotech 9 (411), 1-12 , 2019 2019 Citations: 7
