Isolation, identification and molecular characterization of brucella abortus from bovines MD Shrimali, NM Shah, BS Chandel, HC Chauhan, SS Patel, KB Patel, BK Patel, AG Bhagat, SI Patel, AI Dadawala, JD Shah, Manish Rajgor, RP Pandya, AC Patel, MA Patel, JK Kala, MG Patel Journal of Pure and Applied Microbiology, 2017 Brucellosis is an important zoonosis and a significant cause of reproductive losses in animals. Abortion, placentitis, epididymitis and orchitis are the most common clinical manifestations in animals. The present study a total of 168 clinical samples were collected from buffaloes (87) and cattle (81). All clinical samples were processed by cultural isolation on Brucella agar medium (BAM) with selective antibiotic supplements and genus specific PCR using B4/B5 (223bp) and F4/R2 (905bp) primer. Out of 168 clinical samples 15 samples yielded Brucella isolates by cultural isolation and 19 samples positive for Brucella organism by genus specific PCR. All genus specific PCR positive 19 samples also positive by Species specific PCR based on omp31, B. abortus +IS711 (498bp) primer. Amplicon of 498bp by B. abortus +IS711 primers indicates that all nineteen samples of cattle and buffaloes were found to be Brucella abortus.
Detection of virulence associated factors from Staphylococcus aureus isolated from bovine mastitis Buffalo Bulletin, 2016
Complete genome sequence of Brucella abortus SKN 13 isolated from placenta of aborted cattle in Gujarat, India H. C. Chauhan, Bharat K. Patel, B. S. Chandel, Kirit B. Patel, A. C. Patel, M. D. Shrimali, S. S. Patel, A. G. Bhagat, Manish Rajgor, Mitul A. Patel, Maulik Patel, Jitendra Kala, Bhumika Patel Genome Announcements, 2016 Brucella abortus is generally known to cause brucellosis in cattle and buffalo. Here, we report the draft genome sequence of Brucella abortus SKN 13, isolated from aborted cattle placenta in the area of Gujarat, India, providing precious resources for comparative genomic analyses of Brucella field strains.
Isolation, identification and antibiogram of streptococcus agalactiae isolated from the bovine mastitis Indian Veterinary Journal, 2015
Seroprevalence of ovine brucellosis in North Gujarat and kutchh regions of Gujarat by i-ELISA and its comparison with RBPT and STAT Indian Veterinary Journal, 2015
Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle H. C. Chauhan, B. K. Patel, A. G. Bhagat, M. V. Patel, S. I. Patel, S. H. Raval, H. H. Panchasara, M. D. Shrimali, A. C. Patel, B. S. Chandel Veterinary World, 2015 Aim: Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques. Materials and Methods: A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were screened for theileriosis using Giemsa’s staining technique and polymerase chain reaction (PCR). Results: Total of 17 (32.69%) and 24 (46.15%) samples were found positive for theileriosis by microscopic examination and PCR test, respectively. It revealed that the study area is endemic for theileriosis, and the microscopic technique has 70.83% sensitivity and 100% specificity with respect to PCR technique. Conclusion: It may be concluded from the present study that the PCR is comparatively sensitive technique than microscopic examination and may be recommended to use in the field for screening of theileriosis in the study area, where a high prevalence of diseases have been reported due to intensive dairy farming.
Seroprevalence of bluetongue virus antibodies in camels Journal of Camel Practice and Research, 2013
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Enhancing Storage Stability of Pearl Millet Flour through Microwave Treatment JS Patel, AG Bhagat, HN Zala, YR Patel, VA Joshi International Journal of Biochemistry Research & Review 34 (5), 72-81 , 2025 2025
Exploration of Plant Growth-promoting Traits of Diazotroph Isolates Obtained from Rhizospheric Soil P Mevada, A Bhagat, G Dave, J Patel, Y Patel, A Gelot, HS Bhadauria Advances in Research on Teaching 26 (4), 344-357 , 2025 2025
Cyamopsis tetragonoloba and Arachis hypogaea crop residue smoke water characterization and application GS Dave, A Mevada, YR Patel, AG Bhagat, KP Pachchigar, RA Dave, ... Herbal Formulations, Phytochemistry and Pharmacognosy, 261-269 , 2024 2024
Approach towards Submerge Production, Characterization and Application of Extracellular Alkaline Protease from Pseudomonas aeruginosa YPVC V Chaudhary, Y Patel, K Soni, G Dave, A Bhagat, J Patel, A Parmar, ... Biological Forum–An International Journal 13 (4), 1004-1014 , 2021 2021 Citations: 2
Microwave Assisted Green Gram Germination GS Dave, MD Patel, DB Chaudhari, AA Mohapatra, YR Patel, AG Bhagat, ... Proceedings of the National Conference on Innovations in Biological Sciences … , 2020 2020
Seroprevalence of brucellosis in bovine M Shrimali, S Patel, H Chauhan, BS Chandel, AC Patel, K Sharma, ... International Journal of Current Microbiology and Applied Sciences 8 (11 … , 2019 2019 Citations: 6
Detection of Bluetongue Virus Antigen in Various Samples from Gujarat State, India AI Dadawala, B Mondal, HN Kher, HC Chauhan, AG Bhagat, MD Shrimali, ... Society for Immunology and Immunopathology , 2019 2019
Phenotypic and Genotypic Identification of Methicillin-Resistant Coagulase Negative Staphylococcus spp. Isolated from Bovine Mastitis AG Bhagat, HN Kher, AI Dadawala, HC Chauhan, MD Shrimali, KB Patel, ... Int. J. Curr. Microbiol. App. Sci 7 (3), 1110-1120 , 2018 2018 Citations: 2
Isolation, identification and molecular characterization of Brucella abortus from bovines MD Shrimali, NM Shah, BS Chandel, HC Chauhan, SS Patel, KB Patel, ... Journal of Pure and Applied Microbiology 11 (2), 933-939 , 2017 2017 Citations: 4
Comparative study on cultural and molecular methods for detection of Brucella melitensis in sheep and goat KB Patel, SI Patel, BK Patel, HC Chauhan, AG Bhagat, BS Chandel Ruminant Science 6 (2), 309-314 , 2017 2017 Citations: 9
Ideal Sample for Isolation of Pasteurella multocida and Susceptibility of Field Isolates Against Commonly Used Antibiotics HC Chauhan, AG Bhagat, KP Bharat, MV Patel, MD Shrimali, ... Journal of Immunology and Immunopathology 19 (1), 42-46 , 2017 2017
Serological survey of brucellosis in camel of Gujarat HC Chauhan, KB Patel, SI Patel, BK Patel, BS Chandel, AG Bhagat, ... Int. J. Curr. Microbiol. Appl. Sci 6 (4), 1815-1821 , 2017 2017 Citations: 10
Identification and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis AG Bhagat, HN Kher, AI Dadawala, HC Chauhan, BK Patel, MD Shrimali, ... International Journal of Current Microbiology and Applied Sciences 6 (2 … , 2017 2017 Citations: 9
Detection of virulence associated factors from Staphylococcus aureus isolated from bovine mastitis FM Parth, HC Chauhan, AG Bhagat, BS Chandel, AI Dadawala, HN Kher Buffalo bulletin 35 (4), 687-696 , 2016 2016 Citations: 15
Complete genome sequence of Brucella abortus SKN 13 isolated from placenta of aborted cattle in Gujarat, India HC Chauhan, BK Patel, BS Chandel, KB Patel, AC Patel, MD Shrimali, ... Genome Announcements 4 (5), 10.1128/genomea. 01123-16 , 2016 2016 Citations: 3
Seroepidemiology, molecular and entomological studies of bluetongue in sheep in Gujarat HC Chauhan, AG Bhagat, AI Dadawala, BK Patel, BS Chandel, HN Kher, ... African Journal of Microbiology Research, 856-860 , 2016 2016 Citations: 1
Isolation, Identification and Antibiogram of Staphylococcus aureus Isolated from Bovine Mastitis B Patel Kirit, HN Kher Journal of Immunology and Immunopathology 18 (1), 58-62 , 2016 2016 Citations: 1
Comparative efficacy of antibiotic sensitivity tests for management of acute clinical Escherichia coli mastitis in crossbred cow. PM Chauhan, HK Thumar, A Bhagat, VK Sharma, HC Chauhan, MR Patel 2016 Citations: 8
Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle HC Chauhan, BK Patel, AG Bhagat, MV Patel, SI Patel, SH Raval, ... Veterinary world 8 (11), 1370 , 2015 2015 Citations: 48
Seroprevalence of ovine brucellosis in North Gujarat and Kutchh regions of Gujarat by i-ELISA and its comparison with RBPT and STAT AJ Kotadiya, P Kumar, KM Patel, MV Patel, SI Shroff, AI Dadawala, ... Indian Veterinary Journal 92 (11), 42-46 , 2015 2015 Citations: 8
MOST CITED SCHOLAR PUBLICATIONS
Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle HC Chauhan, BK Patel, AG Bhagat, MV Patel, SI Patel, SH Raval, ... Veterinary world 8 (11), 1370 , 2015 2015 Citations: 48
Incidence of Mycobacterium avium subspecies paratuberculosis in Mehsani and Surti goats of Indian origin using multiple diagnostic tests. DB Barad, BS Chandel, AI Dadawala, HC Chauhan, HS Kher, S Shroff, ... 2014 Citations: 22
Isolation and molecular characterization of bluetongue virus 16 of goat origin from India AI Dadawala, HS Kher, BS Chandel, AG Bhagat, HC Chauhan, K Ranjan, ... Advances in Animal and Veterinary Sciences, 24-29 , 2013 2013 Citations: 18
Detection of virulence associated factors from Staphylococcus aureus isolated from bovine mastitis FM Parth, HC Chauhan, AG Bhagat, BS Chandel, AI Dadawala, HN Kher Buffalo bulletin 35 (4), 687-696 , 2016 2016 Citations: 15
Evaluation of Indigenous vaccine in Patanwadi sheep naturally infected with clinical Johne’s disease S Shroff, BS Chandel, AI Dadawala, SV Singh, AG Bhagat, HC Chauhan, ... Res. Opin. Anim. Vet. Sci 3 (9), 322-329 , 2013 2013 Citations: 13
Comparative potential of traditional versus modern diagnostic tests in estimating status of caprine johne’s disease DS Barad, BS Chandel, A Dadawala, HC Chauhan, HS Kher, S Shroff, ... Advances in Animal and Veterinary Sciences 1 (1), 35-40 , 2013 2013 Citations: 11
Detection of Brucella antibody by using different serological tests, in cattle of North Gujarat. DAI Chandel B.S. , Chauhan H.C., Pankaj Kumar., Bhagat A.G., Shah N.M. Ruminant Science 1 (2), 145-148 , 2012 2012 Citations: 11
Serological survey of brucellosis in camel of Gujarat HC Chauhan, KB Patel, SI Patel, BK Patel, BS Chandel, AG Bhagat, ... Int. J. Curr. Microbiol. Appl. Sci 6 (4), 1815-1821 , 2017 2017 Citations: 10
Comparative study on cultural and molecular methods for detection of Brucella melitensis in sheep and goat KB Patel, SI Patel, BK Patel, HC Chauhan, AG Bhagat, BS Chandel Ruminant Science 6 (2), 309-314 , 2017 2017 Citations: 9
Identification and characterization of methicillin-resistant Staphylococcus aureus isolated from bovine mastitis AG Bhagat, HN Kher, AI Dadawala, HC Chauhan, BK Patel, MD Shrimali, ... International Journal of Current Microbiology and Applied Sciences 6 (2 … , 2017 2017 Citations: 9
Molecular detection of bovine rotavirus from North Gujarat, India. AI Dadawala, BS Chandel, HC Chauhan, AG Bhagat 2013 Citations: 9
Comparative efficacy of antibiotic sensitivity tests for management of acute clinical Escherichia coli mastitis in crossbred cow. PM Chauhan, HK Thumar, A Bhagat, VK Sharma, HC Chauhan, MR Patel 2016 Citations: 8
Seroprevalence of ovine brucellosis in North Gujarat and Kutchh regions of Gujarat by i-ELISA and its comparison with RBPT and STAT AJ Kotadiya, P Kumar, KM Patel, MV Patel, SI Shroff, AI Dadawala, ... Indian Veterinary Journal 92 (11), 42-46 , 2015 2015 Citations: 8
Seroprevalence of brucellosis in bovine M Shrimali, S Patel, H Chauhan, BS Chandel, AC Patel, K Sharma, ... International Journal of Current Microbiology and Applied Sciences 8 (11 … , 2019 2019 Citations: 6
Isolation, identification and molecular characterization of Brucella abortus from bovines MD Shrimali, NM Shah, BS Chandel, HC Chauhan, SS Patel, KB Patel, ... Journal of Pure and Applied Microbiology 11 (2), 933-939 , 2017 2017 Citations: 4
Evaluation of an'Indigenous vaccine'based on goat adapted Mycobacterium avium subspecies paratuberculosis in Patanwadi breed of sheep naturally infected with clinical Johne's … S Shroff, BS Chandel, AI Dadawala, SV Singh, AG Bhagat, HC Chauhan, ... Research Opinions in Animal & Veterinary Sciences 3 (9) , 2013 2013 Citations: 4
Complete genome sequence of Brucella abortus SKN 13 isolated from placenta of aborted cattle in Gujarat, India HC Chauhan, BK Patel, BS Chandel, KB Patel, AC Patel, MD Shrimali, ... Genome Announcements 4 (5), 10.1128/genomea. 01123-16 , 2016 2016 Citations: 3
Isolation, identification and antibiogram of Streptococcus agalactiae Isolated from the Bovine Mastitis GH Bhagat, AI Kher, HC Dadawala, BS Chauhan, NM Shah Indian Vet. J 11 , 2015 2015 Citations: 3
Seroprevalence of bluetongue virus antibodies in goats of Gujarat AG Bhagat, BS Chandel, AI Dadawala, HC Chauhan, VA Pathan Indian Journal of Small Ruminants (The), 134-137 , 2014 2014 Citations: 3
Approach towards Submerge Production, Characterization and Application of Extracellular Alkaline Protease from Pseudomonas aeruginosa YPVC V Chaudhary, Y Patel, K Soni, G Dave, A Bhagat, J Patel, A Parmar, ... Biological Forum–An International Journal 13 (4), 1004-1014 , 2021 2021 Citations: 2