Green Synthesis of Zinc and Iron Nanoparticles Using Psidium guajava Leaf Extract Stimulates Cowpea Growth, Yield, and Tolerance to Saline Water Irrigation Mohamed H. Sheta, Ahmed H. M. Abd El-Wahed, Mohammed A. Elshaer, Hala M. Bayomy, Nawal A. Ozaybi, Mohamed A. M. Abd-Elraheem, Abdel-Nasser A. El-Sheshtawy, Rasha S. El-Serafy, Mahmoud M. I. Moustafa Horticulturae, 2024 Plants use a variety of physiological, biochemical, and molecular mechanisms to mitigate salt stress impacts. Many techniques, including the application of nanoparticles (NPs), are being used to increase plant stress tolerance. To assess the growth and productivity of Vigna unguiculata L. (cowpea) plants exposed to salt stress, cowpea has been cultivated using different saline water levels and subjected to green synthesized zinc NPs (ZnNPs) and iron NPs (FeNPs) applied via foliar spraying. The cowpea plants that grew under the lowest saline water level showed the best leaf traits, leaf water content per area (LWCA), pods, and seed yields, but when salinity levels increased, the plants’ growth and productivity slightly declined. ZnNP and FeNP treatments slow down the degradation of photosynthetic pigments and greatly mitigate the negative effects of salt stress. In both stressed and unstressed plants, ZnNP treatments produced the highest osmoprotectant concentrations (proline, protein, and total carbohydrates). As a result of salt stress, cowpea seeds showed a marked decrease in dry matter and protein content, but ZnNP and FeNP treatments increased it. Conclusively, the results obtained indicated that ZnNPs and FeNPs foliar application to cowpea plants stimulated leaf pigment and polyphenol production, which in turn increased seed dry matter, seed yield, protein content, and the plants’ ability to withstand saline stress.
Improved Water Use Efficiency and Yield of Drip-Irrigated Pepper under Full and Deficit Irrigation Conditions Mahmoud Mostafa, Ahmed Abd El-wahed, Saleh Hamad, Mohamed Sheta Egyptian Journal of Soil Science, 2024 In Egypt, water scarcity is the main problem the agricultural sector faces. Salicylic acid (SA) as a plant growth regulator may help mitigate the adverse effects of deficit irrigation water on pepper plants. Sweet pepper (cv. S702 F1) plants subjected to deficit irrigation (DI40%= 60% of crop evapotranspiration, ETc) regularly during growth stages produced decreases in plant height, total chlorophyll, fruit number per plant, length, diameter, volume, yield, carotenoids, and total dry matter comparing with the full irrigation (FI= 100% of ETc). However, water use efficiency (WUE), proline, free amino acids, ascorbic acid, total sugars, total soluble solids (TSS), total phenols content (TPC), total flavonoids content (TFC), and nitrate content were enhanced under deficit irrigation conditions. The results showed that under FI or DI40% with foliar application of SA at 1.0 mM was more effective than 0.5 and 1.5 mM in improving vegetative growth and yield parameters. However, FI produced the highest significant values of plant height, total chlorophyll, fruit yield, carotenoids, and total dry matter. Conversely, the pepper plant's ability to withstand DI40% was enhanced by the 1.0 mM SA treatment, which resulted in the highest accumulation of ascorbic acid, total sugars, TSS, TPC, and TFC. Moreover, the application of SA significantly increased WUE and decreased fruit nitrate content. It could be concluded that the foliar application of SA can alleviate the detrimental effects of deficit irrigation on pepper plants.
Response of Yield, Quality, and Bioactive Constituents of Green Onion to Foliar Spraying with Moringa oleifera Leaf Extract and Yeast Extract Asaad Awad, Ahmed Abd El-Wahed, Ahmed Elateeq, Mahmoud Moustafa, Shakir Ullah, Mostafa Zarad, Ali Nofal, Saleh Hamad, Ramy Nada, Taghreed Badawi Egyptian Journal of Soil Science, 2024 The application of biostimulants to improve plant growth, quality, and productivity is an effective and eco-friendly field. Green onion is a widely consumed vegetable due to its nutritional content and distinctive flavor. The response of green onion growth, yield, bioactive compounds accumulation, and antioxidant activity to foliar application of moringa (Moringa oleifera) leaf extract (MLE) and yeast extract (YE), as biostimulants was investigated in the present study during 2021/2022 and 2022/2023 seasons under open field conditions. MLE (2%, 4%, and 6%) and YE (1, 2, and 3 g/L) were applied as foliar solutions. The results showed that plant height, fresh weight (FW), dry matter, yield, nutrients content, photosynthetic pigments (chlorophyll and carotenoids), bioactive compounds [ascorbic acid, total phenolics content (TPC), and total flavonoids content (TFC)], and antioxidant properties [total antioxidant capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity (IC50)] exhibited a better response to specific levels of YE and MLE. When compared to chemical fertilizers, the increase in green onion yield achieved by YE and MLE was accompanied by a decrease in nitrate content and an increase in total soluble solids (TSS), pyruvic acid, and ascorbic acid. TPC was maximized with chemical fertilizers and 2 g/L of YE. However, no statistical differences were observed in the TFC, TAC, and IC50 among treatments involving chemical fertilizers, moderate, and higher levels of MLE and YE. According to Pearson's correlation analysis, a positive correlation existed between yield and each of ascorbic acid, TPC, TFC, and TAC. The nitrate content correlated negatively with pseudo-stem length, TSS, pyruvic acid, and bioactive compounds. Conclusively, YE and MLE have the potential to make green onion farming more sustainable and resilient with high quality and high nutraceutical content, thus mitigating the need for chemical fertilizers.
Morpho-physiological traits, quality and productivity of garlic under drought stress of different growth stages Mahmoud Moustafa, Ahmed Abd El-wahed, Asaad Awad, Mohamed Sheta Egyptian Journal of Soil Science, 2024 Due to the limited water supply and rising food demand, studying how applied irrigation rates affect crops at this critical time due to climate change is essential. Therefore, an experiment was carried out to examine the effect of different irrigation water rates, specifically 60, 80, 100, and 120 of the water requirements (WR), on the growth, bulb yield properties, and water status of garlic (Allium sativum L.) clone Sids-40. The data was recorded after 80 − 140 days from planting, where polynomial regression analysis was estimated between the growth stages and growth parameters. Similarly, 100% of the WR considerably increases plant height, leaf number, leaf fresh weight, and growth attributes like absolute growth rate, specific leaf area, leaf area index, chlorophyll a, b, and total carotenoids contents. While the irrigation at a rate of 60% WR led to an intrinsic rise in leaf water saturation deficit, proline content, and ascorbic acid content. Bulb parameters such as weight, diameter, and number of cloves per bulb, besides yield quantity with bulb grade one G1 (above 5.5 cm), pyruvic acid as pungency, and dry matter contents were determined to be at their highest values with irrigation at 100% of the WR. A 60% of the WR water shortage represented the high yield reduction with grade 4 (less than 3.5 cm). Pearson's correlation revealed positive relationships between bulb yield, characteristics, and growth attributes. Hence, garlic plants respond to irrigation at 100% of the WR by improving their morpho-physiological, productivity and bulb quality.
Evaluation of Salicylic Acid Effects on Growth, Biochemical, Yield, and Anatomical Characteristics of Eggplant (Solanum melongena L.) Plants under Salt Stress Conditions Emad Mady, Ahmed H. M. Abd El-Wahed, Asaad H. Awad, Turky O. Asar, Ammar Al-Farga, Hany S. Abd El-Raouf, Reena Randhir, Ehab S. Alnuzaili, Ahmed M. El-Taher, Timothy O. Randhir, Fatma A. Hamada Agronomy, 2023 Salt stress is a major issue in agriculture and crop production that influences global food security. Mitigation options to address salt stress through agronomic practices can help manage this issue. Experiments were performed in two summer seasons in an experimental farm to test the impact of three salinity levels (S): 300 (control), 1000, 2000, and 3000 ppm, and two salicylic acid (SA) levels, including 1.0 and 1.50 mM, and their interaction on growth and yield of eggplant (Solanum melongena L.) hybrid Suma. The results showed that increasing S levels up to 3000 ppm reduced plant and fruit physical characteristics, as well as leaf and fruit chemical characteristics, especially leaf total chlorophyll, carotenoids, relative water, fruit nitrogen, phosphorus, and potassium contents, which led to a reduction in total yield per plant. However, an insignificant effect was observed in the control level and 1000 ppm saline water in leaf area, fruit length, leaf total chlorophyll content, fruit phosphorus content, and total yield per plant. In contrast, leaf sugars, proline contents, electrolyte leakage, fruit TSS (total soluble solids), and ascorbic acid contents were improved with S levels up to the concentration of 3000 ppm compared to the control. However, tested parameters were significantly higher due to the SA foliar spray of 1.0 mM besides photosynthetic pigments of leaves enhanced by using 1.0 and 1.50 mM. Using 1.0 mM SA concentration alleviated the adverse impact of S on eggplant plants until 1000 ppm saline water, reflecting an increase in eggplant yield. The anatomical structure of eggplant leaves revealed positive variations in mature leaf blades in both the stressed and SA-treated plants. Based on these results, the use of SA at a concentration of 1.0 mM may lessen the negative impacts of salt on the growth of eggplant, which increases the overall yield.