Katerina Komrskova

@ibt.cas.cz

Reproductive Biology
Institute of Biotechnology of the Czech Academy of Sciences, Czech Republic

Katerina Komrskova


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https://researchid.co/a-8705-2010

RESEARCH INTERESTS

Selecting markers associated with human infertility and designing diagnostic kits for clinical practice, sperm-egg interaction and fusion; sperm preparation for fertilization (capacitation and acrosome reaction); sperm-egg interaction; epigenetic regulation of early embryogenesis, epigenetic markers associated with human infertility; spermatogenesis and sperm parameters assessment after pathological challenge including cancer, genomic and proteomic approach.
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Scopus Publications

Scopus Publications

  • Proteomic analysis of the mouse sperm acrosome - towards an understanding of an organelle with diverse functionality
    Tereza Otčenášková, Eliška Macíčková, Jana Vondráková, Michaela Frolíková, Katerina Komrskova, Romana Stopková, Pavel Stopka
    European Journal of Cell Biology, 2023
    The acrosome located within the mammalian sperm head is essential for successful fertilization, as it enables the sperm to penetrate the extracellular layers of the oocyte and fuse with oolemma. However, the mammalian acrosomal vesicle is no longer considered to contain only hydrolytic enzymes. Using label-free nano-scale liquid chromatography tandem mass spectrometry (nLC-MS/MS) proteomics, we identified a total of 885 proteins in the acrosome isolated from spermatozoa obtained from cauda epididymis of free-living house mice Mus musculus musculus contains a total of 885 proteins. Among these, 334 proteins were significantly enriched in the acrosome thus representing 27.3% of the whole proteome of the intact sperm. Importantly, we have detected a total of nine calycins while eight of them belong to the lipocalin protein family. In mice, lipocalins are involved in multi-level chemical communication between individuals including pheromone transport and odor perception. Using an indirect immunofluorescence assay, we demonstrated that lipocalin 5 (LCN5) is expressed in the mouse germ cells, and after completing spermatogenesis, it remains localized in the sperm acrosome until the last step of the extratesticular maturation, the acrosome reaction. The presence of lipocalins in the acrosome and acrosome-reacted sperm suggests their original role as chelators of organic and potentially toxic compounds resulting from ongoing spermiogenesis. Along with this evidence, detected mitochondrial (e.g., a subunit of the cytochrome c oxidase MTCO1) and proteasomal proteins (subunits of both 20 S core proteasome [PSMA2, PSMBs] and 19 S regulatory particle [PSMDs]) in acrosomes provide further evidence that acrosomes could also function as `waste baskets` after testicular sperm maturation.
  • Timing of ICSI with Respect to Meiotic Spindle Status
    Olga Tepla, Zinovij Topurko, Simona Jirsova, Martina Moosova, Eva Fajmonova, Radek Cabela, Katerina Komrskova, Irena Kratochvilova, Jaromir Masata
    International Journal of Molecular Sciences, 2023
    The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5–6 h after ovum pick-up (OPU) or 7–8 h after OPU. Oocytes without MS evaluation were all fertilized 5–6 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58/182); while 195 patients without MS visualization had 24% PR (47/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58/140), while 162 patients without MS visualization had 41% PR (66/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.
  • Sperm morphology and performance in relation to postmating prezygotic isolation in two recently diverged passerine species
    Manon Poignet, Lucie Baránková, Jiří Reif, Pavel Stopka, Romana Stopková, Michaela Frolikova, Emily R. A. Cramer, Arild Johnsen, Pavel Kverek, Tomasz S. Osiejuk, Katerina Komrskova, Tomáš Albrecht, Radka Reifová
    Scientific Reports, 2022
    Divergence in sperm phenotype and female reproductive environment may be a common source of postmating prezygotic (PMPZ) isolation between species. However, compared to other reproductive barriers it has received much less attention. In this study, we examined sperm morphology and velocity in two hybridizing passerine species, the common nightingale (Luscinia megarhynchos) and thrush nightingale (L. luscinia). In addition, we for the first time characterized a passerine female reproductive tract fluid proteome. We demonstrate that spermatozoa of the common nightingale have significantly longer and wider midpiece (proximal part of the flagellum containing mitochondria) and longer tail compared to spermatozoa of thrush nightingale. On the other hand, they have significantly shorter and narrower acrosome. Importantly, these differences did not have any effect on sperm velocity. Furthermore, the fluid from the reproductive tract of common nightingale females did not differentially affect velocity of conspecific and heterospecific sperm. Our results indicate that the observed changes in the flagellum and acrosome size are unlikely to contribute to PMPZ isolation through differential sperm velocity of conspecific and heterospecific sperm in the female reproductive tract. However, they could affect other postcopulatory processes, which might be involved in PMPZ isolation, such as sperm storage, longevity or sperm-egg interaction.
  • Fertilization, but Not Post-Implantation Development, Can Occur in the Absence of Sperm and Oocyte Beta1 Integrin in Mice
    Nour El Houda Mimouni, Côme Ialy-Radio, Anne-Lyse Denizot, Isabelle Lagoutte, Michaela Frolikova, Katerina Komrskova, Sandrine Barbaux, Ahmed Ziyyat
    International Journal of Molecular Sciences, 2022
    Fertilization is a complex process that requires successive stages and culminates in the adhesion/fusion of gamete membranes. If the question of the involvement of oocyte integrins has been swept away by deletion experiments, that of the involvement of sperm integrins remains to be further characterized. In the present study, we addressed the question of the feasibility of sperm–oocyte adhesion/fusion and early implantation in the absence of sperm β1 integrin. Males and females with β1 integrin-depleted sperm and oocytes were mated, and fertilization outcome was monitored by a gestational ultrasound analysis. Results suggest that although the sperm β1 integrin participates in gamete adhesion/fusion, it is dispensable for fertilization in mice. However, sperm- and/or oocyte-originated integrin β1 is essential for post-implantation development. Redundancy phenomena could be at the origin of a compensatory expression or alternative dimerization pattern.
  • Dynamic study of small toxic hydrophobic proteins PepA1 and PepG1 of Staphylococcus aureus
    Vishma Pratap Sur, Ondrej Simonik, Michaela Novotna, Aninda Mazumdar, Frantisek Liska, Vladimir Vimberg, Katerina Komrskova
    International Journal of Biological Macromolecules, 2022
  • H4K5 Butyrylation Coexist with Acetylation during Human Spermiogenesis and Are Retained in the Mature Sperm Chromatin
    Alberto de la Iglesia, Paula Jauregi, Meritxell Jodar, Ferran Barrachina, Lukas Ded, Carme Mallofré, Leonardo Rodríguez-Carunchio, Juan Manuel Corral, Josep Lluís Ballescà, Katerina Komrskova, Judit Castillo, Rafael Oliva
    International Journal of Molecular Sciences, 2022
    Male germ cells experience a drastic chromatin remodeling through the nucleo-histone to nucleo-protamine (NH-NP) transition necessary for proper sperm functionality. Post-translational modifications (PTMs) of H4 Lys5, such as acetylation (H4K5ac), play a crucial role in epigenetic control of nucleosome disassembly facilitating protamine incorporation into paternal DNA. It has been shown that butyrylation on the same residue (H4K5bu) participates in temporal regulation of NH-NP transition in mice, delaying the bromodomain testis specific protein (BRDT)-dependent nucleosome disassembly and potentially marking retained nucleosomes. However, no information was available so far on this modification in human sperm. Here, we report a dual behavior of H4K5bu and H4K5ac in human normal spermatogenesis, suggesting a specific role of H4K5bu during spermatid elongation, coexisting with H4K5ac although with different starting points. This pattern is stable under different testicular pathologies, suggesting a highly conserved function of these modifications. Despite a drastic decrease of both PTMs in condensed spermatids, they are retained in ejaculated sperm, with 30% of non-colocalizing nucleosome clusters, which could reflect differential paternal genome retention. Whereas no apparent effect of these PTMs was observed associated with sperm quality, their presence in mature sperm could entail a potential role in the zygote.
  • MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization
    Jana Vondrakova, Michaela Frolikova, Lukas Ded, Jiri Cerny, Pavla Postlerova, Veronika Palenikova, Ondrej Simonik, Zuzana Nahacka, Krystof Basus, Eliska Valaskova, Radek Machan, Allan Pacey, Zuzana Holubcova, Pavel Koubek, Zuzana Ezrova, Soojin Park, Ruiwu Liu, Raghavendran Partha, Nathan Clark, Jiri Neuzil, Masahito Ikawa, Kent Erickson, Kit S. Lam, Harry Moore, Katerina Komrskova
    Science Advances, 2022
    Gamete fusion is a critical event of mammalian fertilization. A random one-bead one-compound combinatorial peptide library represented synthetic human egg mimics and identified a previously unidentified ligand as Fc receptor–like 3, named MAIA after the mythological goddess intertwined with JUNO. This immunoglobulin super family receptor was expressed on humanoolemmaand played a major role during sperm-egg adhesion and fusion. MAIA forms a highly stable interaction with the known IZUMO1/JUNO sperm-egg complex, permitting specific gamete fusion. The complexity of the MAIA isotype may offer a cryptic sexual selection mechanism to avoid genetic incompatibility and achieve favorable fitness outcomes.
  • Kinetic Study of 17α-Estradiol Mechanism during Rat Sperm Capacitation
    Tereza Bosakova, Antonin Tockstein, Zuzana Bosakova, Katerina Komrskova
    Molecules, 2022
    17α-Estradiol (αE2) is a natural diastereoisomer of 17β-estradiol (E2). It is well known that αE2 can bind to estrogen receptors. However, its biological activity is less than that of E2 and is species and tissue specific. The goal of our study was to propose the mechanism of αE2 hormonal response in rat sperm during their capacitation in vitro and compare it with a previously studied mouse model. Concentration changes in externally added αE2 during capacitation of rat sperm were monitored by the high-performance liquid chromatographic method with tandem mass spectrometric detection (HPLC-MS/MS). The calculated values of relative concentrations Bt were subjected to kinetic analysis. The findings indicated that αE2 in rat sperm did not trigger autocatalytic reaction, in contrast to the mouse sperm, and that the initiation of the hormone penetration through the sperm plasma membrane was substantially faster in rats.
  • Boar Sperm Cryopreservation Improvement Using Semen Extender Modification by Dextran and Pentaisomaltose
    Ondrej Simonik, Filipa Bubenickova, Lucie Tumova, Michaela Frolikova, Vishma Pratap Sur, Jan Beran, Katerina Havlikova, Lenka Hackerova, Daniela Spevakova, Katerina Komrskova, Pavla Postlerova
    Animals, 2022
    The long-term storage of boar sperm presents an ongoing challenge, and the modification of the cryoprotective compounds in semen extenders is crucial for improving cryopreservation’s success rate. The aim of our study was to reduce the percentage of glycerol in the extender by elimination or substitution with biocompatible, non-toxic polysaccharides. For boar semen extender improvement, we tested a novel modification with the polysaccharides dextran and pentaisomaltose in combination with unique in silico predictive modeling. We targeted the analysis of in vitro qualitative sperm parameters such as motility, viability, mitochondrial activity, acrosome integrity, and DNA integrity. Non-penetrating polysaccharide-based cryoprotective agents interact with sperm surface proteins such as spermadhesins, which are recognized as fertility markers of boar sperm quality. The in silico docking study showed a moderate binding affinity of dextran and pentaisomaltose toward one specific spermadhesin known as AWN, which is located in the sperm plasma membrane. Pentaisomaltose formed a hydrophobic pocket for the AWN protein, and the higher energy of this protein–ligand complex compared with dextran was calculated. In addition, the root mean square deviation (RMSD) analysis for the molecular dynamics (MD) of both polysaccharides and AWN simulation suggests their interaction was highly stable. The in silico results were supported by in vitro experiments. In the experimental groups where glycerol was partially or entirely substituted, the use of pentaisomaltose resulted in improved sperm mitochondrial activity and DNA integrity after thawing when compared with dextran. In this paper, we demonstrate that pentaisomaltose, previously used for cryopreservation in hematopoietic stem cells, represents a promising compound for the elimination or reduction of glycerol in extenders for boar semen cryopreservation. This novel approach, using in silico computer prediction and in vitro testing, represents a promising technique to help identify new cryoprotectants for use in animal breeding or genetic resource programs.
  • Specific inhibition of VanZ-mediated resistance to lipoglycopeptide antibiotics
    Vishma Pratap Sur, Aninda Mazumdar, Vladimir Vimberg, Tommaso Stefani, Ladislav Androvic, Lucie Kracikova, Richard Laga, Zdenek Kamenik, Katerina Komrskova
    International Journal of Molecular Sciences, 2022
    Teicoplanin is a natural lipoglycopeptide antibiotic with a similar activity spectrum as vancomycin; however, it has with the added benefit to the patient of low cytotoxicity. Both teicoplanin and vancomycin antibiotics are actively used in medical practice in the prophylaxis and treatment of severe life-threatening infections caused by gram-positive bacteria, including methicillin-resistant Staphylococcus aureus, Enterococcus faecium and Clostridium difficile. The expression of vancomycin Z (vanZ), encoded either in the vancomycin A (vanA) glycopeptide antibiotic resistance gene cluster or in the genomes of E. faecium, as well as Streptococcus pneumoniae and C. difficile, was shown to specifically compromise the antibiotic efficiency through the inhibition of teicoplanin binding to the bacterial surface. However, the exact mechanisms of this action and protein structure remain unknown. In this study, the three-dimensional structure of VanZ from E. faecium EnGen0191 was predicted by using the I-TASSER web server. Based on the VanZ structure, a benzimidazole based ligand was predicted to bind to the VanZ by molecular docking. Importantly, this new ligand, named G3K, was further confirmed to specifically inhibit VanZ-mediated resistance to teicoplanin in vivo.
  • Role of integrins in sperm activation and fertilization
    Veronika Merc, Michaela Frolikova, Katerina Komrskova
    International Journal of Molecular Sciences, 2021
  • In silico identification and validation of organic triazole based ligands as potential inhibitory drug compounds of sars-cov-2 main protease
    Vishma Pratap Sur, Madhab Kumar Sen, Katerina Komrskova
    Molecules, 2021
  • αV Integrin Expression and Localization in Male Germ Cells
    Veronika Palenikova, Michaela Frolikova, Eliska Valaskova, Pavla Postlerova, Katerina Komrskova
    International Journal of Molecular Sciences, 2021
  • Important parameters affecting quality of vitrified donor oocytes
    Olga Teplá, Zinovij Topurko, Jaromír Mašata, Simona Jirsová, Michaela Frolíková, Kateřina Komrsková, Adela Minks, Jaroslav Turánek, Anna Lynnyk, Irena Kratochvílová
    Cryobiology, 2021
  • Kinetic study of 17α-estradiol activity in comparison with 17β-estradiol and 17α-ethynylestradiol
    Tereza Bosakova, Antonin Tockstein, Zuzana Bosakova, Katerina Komrskova
    Catalysts, 2021
  • Erratum: Platelets facilitate the wound-healing capability of mesenchymal stem cells by mitochondrial transfer and metabolic reprogramming (Cell Metabolism (2021) 33(2) (283–299.e9), (S1550413120306616), (10.1016/j.cmet.2020.12.006))
    Jennyfer Levoux, Alexandre Prola, Peggy Lafuste, Marianne Gervais, Nathalie Chevallier, Zeynab Koumaiha, Kaouthar Kefi, Laura Braud, Alain Schmitt, Azzedine Yacia, Aurélie Schirmann, Barbara Hersant, Mounia Sid-Ahmed, Sabrina Ben Larbi, Katerina Komrskova, Jakub Rohlena, Frederic Relaix, Jiri Neuzil, Anne-Marie Rodriguez
    Cell Metabolism, 2021
  • Platelets Facilitate the Wound-Healing Capability of Mesenchymal Stem Cells by Mitochondrial Transfer and Metabolic Reprogramming
    Jennyfer Levoux, Alexandre Prola, Peggy Lafuste, Marianne Gervais, Nathalie Chevallier, Zeynab Koumaiha, Kaouthar Kefi, Laura Braud, Alain Schmitt, Azzedine Yacia, Aurélie Schirmann, Barbara Hersant, Mounia Sid-Ahmed, Sabrina Ben Larbi, Katerina Komrskova, Jakub Rohlena, Frederic Relaix, Jiri Neuzil, Anne-Marie Rodriguez
    Cell Metabolism, 2021
  • Genetic Association in the Maintenance of the Mitochondrial Microenvironment and Sperm Capacity
    Hwang I. S. Thomas, Ying-Shiuan Chen, Ching-Han Hung, Dilip Bhargava Sreerangaraja Urs, Tien-Ling Liao, Yen-Chun Lai, Katerina Komrskova, Pavla Postlerová, Yung-Feng Lin, Shu-Huei Kao
    Oxidative Medicine and Cellular Longevity, 2021
  • The role of the linc complex in sperm development and function
    Vera Kmonickova, Michaela Frolikova, Klaus Steger, Katerina Komrskova
    International Journal of Molecular Sciences, 2020
  • Expression and distribution of CD151 as a partner of alpha6 integrin in male germ cells
    J. Jankovicova, M. Frolikova, V. Palenikova, E. Valaskova, J. Cerny, P. Secova, M. Bartokova, L. Horovska, P. Manaskova-Postlerova, J. Antalikova, K. Komrskova
    Scientific Reports, 2020
  • Correction to: Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos (Medical Microbiology and Immunology, (2020), 209, 4, (407-425), 10.1007/s00430-020-00676-0)
    Jana Jankovičová, Zdeňka Neuerová, Petra Sečová, Michaela Bartóková, Filipa Bubeníčková, Kateřina Komrsková, Pavla Postlerová, Jana Antalíková
    Medical Microbiology and Immunology, 2020
  • Gestational and pubertal exposure to low dose of di-(2-ethylhexyl) phthalate impairs sperm quality in adult mice
    Pavla Dostalova, Eva Zatecka, Lukas Ded, Fatima Elzeinova, Eliska Valaskova, Alena Kubatova, Vlasta Korenkova, Lucie Langerova, Katerina Komrskova, Jana Peknicova
    Reproductive Toxicology, 2020
  • Tetraspanins in mammalian reproduction: spermatozoa, oocytes and embryos
    Jana Jankovičová, Zdeňka Neuerová, Petra Sečová, Michaela Bartóková, Filipa Bubeníčková, Kateřina Komrsková, Pavla Postlerová, Jana Antalíková
    Medical Microbiology and Immunology, 2020
  • Mitochondrial function in modulating human granulosa cell steroidogenesis and female fertility
    Dilip Bhargava Sreerangaraja Urs, Wen-Han Wu, Katerina Komrskova, Pavla Postlerova, Yung-Feng Lin, Chii-Ruey Tzeng, Shu-Huei Kao
    International Journal of Molecular Sciences, 2020
  • The role of taste receptor mTAS1R3 in chemical communication of gametes
    Michaela Frolikova, Tereza Otcenaskova, Eliska Valasková, Pavla Postlerova, Romana Stopkova, Pavel Stopka, Katerina Komrskova
    International Journal of Molecular Sciences, 2020
  • Kinetic model of the action of 17α-ethynylestradiol on the capacitation of mouse sperm, monitored by HPLC-MS/MS
    Tereza Bosakova, Antonin Tockstein, Natasa Sebkova, Radomir Cabala, Katerina Komrskova
    Catalysts, 2020
  • Fluorescent analysis of boar sperm capacitation process in vitro
    Lukas Ded, Pavla Dostalova, Eva Zatecka, Andrej Dorosh, Katerina Komrskova, et al.
    Reproductive Biology and Endocrinology, 2019
  • Addressing the compartmentalization of specific integrin heterodimers in Mouse sperm
    Michaela Frolikova, Eliska Valaskova, Jiri Cerny, Audrey Lumeau, Natasa Sebkova, Veronika Palenikova, Noemi Sanches-Hernandez, Alzbeta Pohlova, Pavla Manaskova-Postlerova, Katerina Dvorakova-Hortova
    International Journal of Molecular Sciences, 2019
  • Detection of CD9 and CD81 tetraspanins in bovine and porcine oocytes and embryos
    Jana Jankovicova, Petra Secova, Pavla Manaskova-Postlerova, Ondrej Simonik, Michaela Frolikova, Eva Chmelikova, Lubica Horovska, Katarina Michalkova, Katerina Dvorakova-Hortova, Jana Antalikova
    International Journal of Biological Macromolecules, 2019
  • Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer Cells
    Martina Bajzikova, Jaromira Kovarova, Ana R. Coelho, Stepana Boukalova, Sehyun Oh, Katerina Rohlenova, David Svec, Sona Hubackova, Berwini Endaya, Kristyna Judasova, Ayenachew Bezawork-Geleta, Katarina Kluckova, Laurent Chatre, Renata Zobalova, Anna Novakova, Katerina Vanova, Zuzana Ezrova, Ghassan J. Maghzal, Silvia Magalhaes Novais, Marie Olsinova, Linda Krobova, Yong Jin An, Eliska Davidova, Zuzana Nahacka, Margarita Sobol, Teresa Cunha-Oliveira, Cristian Sandoval-Acuña, Hynek Strnad, Tongchuan Zhang, Thanh Huynh, Teresa L. Serafim, Pavel Hozak, Vilma A. Sardao, Werner J.H. Koopman, Miria Ricchetti, Paulo J. Oliveira, Frantisek Kolar, Mikael Kubista, Jaroslav Truksa, Katerina Dvorakova-Hortova, Karel Pacak, Robert Gurlich, Roland Stocker, Yaoqi Zhou, Michael V. Berridge, Sunghyouk Park, Lanfeng Dong, Jakub Rohlena, Jiri Neuzil
    Cell Metabolism, 2019
  • Mouse in vitro spermatogenesis on alginate-based 3D bioprinted scaffolds
    Yoni Baert, Katerina Dvorakova-Hortova, Hasmik Margaryan, Ellen Goossens
    Biofabrication, 2019
  • New insight into sperm capacitation: A novel mechanism of 17β-estradiol signalling
    Tereza Bosakova, Antonin Tockstein, Natasa Sebkova, Ondrej Simonik, Hana Adamusova, Jana Albrechtova, Tomas Albrecht, Zuzana Bosakova, Katerina Dvorakova-Hortova
    International Journal of Molecular Sciences, 2018
  • CD9 and CD81 interactions and their structural modelling in sperm prior to fertilization
    Michaela Frolikova, Pavla Manaskova-Postlerova, Jiri Cerny, Jana Jankovicova, Ondrej Simonik, Alzbeta Pohlova, Petra Secova, Jana Antalikova, Katerina Dvorakova-Hortova
    International Journal of Molecular Sciences, 2018
  • Of oestrogens and sperm: A review of the roles of oestrogens and oestrogen receptors in male reproduction
    Pavla Dostalova, Eva Zatecka, Katerina Dvorakova-Hortova
    International Journal of Molecular Sciences, 2017
  • Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
    Lan-Feng Dong, Jaromira Kovarova, Martina Bajzikova, Ayenachew Bezawork-Geleta, David Svec, Berwini Endaya, Karishma Sachaphibulkij, Ana R Coelho, Natasa Sebkova, Anna Ruzickova, An S Tan, Katarina Kluckova, Kristyna Judasova, Katerina Zamecnikova, Zuzana Rychtarcikova, Vinod Gopalan, Ladislav Andera, Margarita Sobol, Bing Yan, Bijay Pattnaik, Naveen Bhatraju, Jaroslav Truksa, Pavel Stopka, Pavel Hozak, Alfred K Lam, Radislav Sedlacek, Paulo J Oliveira, Mikael Kubista, Anurag Agrawal, Katerina Dvorakova-Hortova, Jakub Rohlena, Michael V Berridge, Jiri Neuzil
    Elife, 2017
  • Characterization of CD46 and β1 integrin dynamics during sperm acrosome reaction
    Michaela Frolikova, Natasa Sebkova, Lukas Ded, Katerina Dvorakova-Hortova
    Scientific Reports, 2016
  • Mouse lipocalins (MUP, OBP, LCN) are co-expressed in tissues involved in chemical communication
    Romana Stopková, David Vinkler, Barbora Kuntová, Ondrej Šedo, Tomáš Albrecht, Jan Suchan, Kateřina Dvořáková-Hortová, Zbyněk Zdráhal, Pavel Stopka
    Frontiers in Ecology and Evolution, 2016
  • Kinetic analysis of decreased sperm fertilizing ability by fluorides and fluoroaluminates: a tool for analyzing the effect of environmental substances on biological events
    Zuzana Bosakova, Antonin Tockstein, Hana Adamusova, Pavel Coufal, Natasa Sebkova, Katerina Dvorakova-Hortova
    European Biophysics Journal, 2016
  • Methylation analysis of histone h4k12ac-associated promoters in sperm of healthy donors and subfertile patients
    Markus Vieweg, Katerina Dvorakova-Hortova, Barbora Dudkova, Przemyslaw Waliszewski, Marie Otte, Berthold Oels, Amir Hajimohammad, Heiko Turley, Martin Schorsch, Hans-Christian Schuppe, Wolfgang Weidner, Klaus Steger, Agnieszka Paradowska-Dogan
    Clinical Epigenetics, 2015
  • Toxoplasma gondii decreases the reproductive fitness in mice
    Katerina Dvorakova-Hortova, Adela Sidlova, Lukas Ded, Denisa Hladovcova, Markus Vieweg, Wolfgang Weidner, Klaus Steger, Pavel Stopka, Agnieszka Paradowska-Dogan
    Plos One, 2014
  • Sperm-egg fusion: A molecular enigma of mammalian reproduction
    Karolina Klinovska, Natasa Sebkova, Katerina Dvorakova-Hortova
    International Journal of Molecular Sciences, 2014
  • Progress of sperm IZUMO1 relocation during spontaneous acrosome reaction
    Natasa Sebkova, Lukas Ded, Katerina Vesela, Katerina Dvorakova-Hortova
    Reproduction, 2014
  • In vivo exposure to 17β-estradiol triggers premature sperm capacitation in cauda epididymis
    Lukas Ded, Natasa Sebkova, Martina Cerna, Fatima Elzeinova, Pavla Dostalova, Jana Peknicova, Katerina Dvorakova-Hortova
    Reproduction, 2013
  • The slower the better: How sperm capacitation and acrosome reaction is modified in the presence of estrogens
    Natasa Sebkova, Martina Cerna, Lukas Ded, Jana Peknicova, Katerina Dvorakova-Hortova
    Reproduction, 2012
  • Erratum: The morphology of the squirrel spermatozoon: A highly complex male gamete with a massive acrosome
    William G. Breed, Serica Tan, Chrisopher M. Leigh, Ken P. Aplin, Katerina Dvorakova-Hortova, Harry D.M. Moore
    Journal of Morphology, 2012
  • Role of complement regulatory proteins CD46, CD55 and CD59 in reproduction
    Michaela Frolíková, Romana Stopková, Jana Antalíková, Peter M. Johnson, Pavel Stopka, Kateřina Dvořáková-Hortová
    Folia Zoologica, 2012
  • Genome wide identification of promoter binding sites for H4K12ac in human sperm and its relevance for early embryonic development
    Agnieszka S. Paradowska, David Miller, Andrej-Nikolai Spiess, Markus Vieweg, Martina Cerna, Katerina Dvorakova-Hortova, Marek Bartkuhn, Hans-Christian Schuppe, Wolfgang Weidner, Klaus Steger
    Epigenetics, 2012
  • The morphology of the squirrel spermatozoon: A highly complex male gamete with a massive acrosome
    William G. Breed, Serica Tan, Chrisopher M. Leigh, Ken P. Aplin, Katerina Dvorakova-Hortova, Harry D.M. Moore
    Journal of Morphology, 2011
  • Effect of estrogens on boar sperm capacitation in vitro
    Lukas Ded, Pavla Dostalova, Andriy Dorosh, Katerina Dvorakova-Hortova, Jana Peknicova
    Reproductive Biology and Endocrinology, 2010
  • Ecological genetics meets epigenetics: Collection of papers from the 1st Central European Meeting on Mouse Epigenetics
    Folia Zoologica, 2009
  • Residual concentration of estriol during mouse sperm capacitation in vitro determined by HPLC method
    Folia Zoologica, 2009
  • CD55 and CD59 protein expression by Apodemus (field mice) sperm in the absence of CD46
    Leanne E. Clift, Katerina Dvorakova-Hortova, Michaela Frolikova, Petra Andrlikova, Suhair Salman, Pavel Stopka, Brian F. Flanagan, Peter M. Johnson
    Journal of Reproductive Immunology, 2009
  • Absence of spermatozoal CD46 protein expression and associated rapid acrosome reaction rate in striped field mice (Apodemus agrarius)
    Leanne E Clift, Petra Andrlikova, Michaela Frolikova, Pavel Stopka, Josef Bryja, et al.
    Reproductive Biology and Endocrinology, 2009
  • The influence of fluorides on mouse sperm capacitation
    K. Dvořáková-Hortová, M. Šandera, M. Jursová, J. Vašinová, J. Pěknicová
    Animal Reproduction Science, 2008
  • Rapid sperm acrosome reaction in the absence of acrosomal CD46 expression in promiscuous field mice (Apodemus)
    Peter M Johnson, Leanne E Clift, Petra Andrlikova, Michaela Jursova, Brian F Flanagan, et al.
    Reproduction, 2007
  • Cytoskeleton localization in the sperm head prior to fertilization
    Kateřina Dvořáková, Harry D M Moore, Nataša Šebková, Jiří Paleček
    Reproduction, 2005
  • Effects of cyanobacterial biomass and purified microcystins on malformations in xenopus laevis: Teratogenesis assay (FETAX)
    Dagmar Dvořáková, Kateřina Dvořáková, Luděk Bláha, Blahoslav Maršálek, Zora Knotková
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  • Depression in the level of cadherin and a-,b-,g-catenins in transgenic Xenopus laevis highly expressing c-Src
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