Dina Eid El-Sayed Rizk

Verified @mans.edu.eg

faculty of pharmacy, Mansoura University, Microbiology and Immunology department

Dina Eid El-Sayed Rizk


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RESEARCH, TEACHING, or OTHER INTERESTS

Immunology and Microbiology
16

Scopus Publications

Scopus Publications

  • Characterization of non-O157 enterohemorrhagic Escherichia coli isolated from different sources in Egypt
    Omnia T. Bahgat, Dina E. Rizk, Hany I. Kenawy, Rasha Barwa
    BMC Microbiology, 2024
    Background Enterohemorrhagic Escherichia coli (EHEC) O157 is implicated in serious food and water-borne diseases as hemorrhagic colitis (HC), and the potentially fatal hemolytic uremic syndrome (HUS). However, new players of non-O157 EHEC have been implicated in serious infections worldwide. This work aims at analyzing serotype and genotypic-based virulence profile of EHEC local isolates. Methods A total of 335 samples were collected from different sources in Egypt. E. coli was isolated and subjected to serotyping. Non-O157 EHEC isolates were tested for virulence genes using PCR, phenotypic examination, phylogenetic typing, and molecular investigation by ERIC typing and MLST to disclose genetic relatedness of isolates. A heat map was used to identify potential associations between the origin of the isolates, their phenotypic and genotypic characteristics. Results A total of 105 out of 335 isolates were identified as E. coli. Surprisingly, 49.5% of these isolates were EHEC, where O111, O91, O26 and O55 were the most prevalent serotypes including 38.46% from stool, 21.15% urine, 23.1% cheese, 9.62% meat products, 3.85% from both yogurt and sewage water. Screening 15 different virulence genes revealed that sheA, stx2 and eae were the most prevalent with abundance rates of 85%, 75% and 36%, respectively. Fifteen profiles of virulence gene association were identified, where the most abundant one was stx2/sheA (19%) followed by stx2/stx2g/sheA/eae (11.5%). Both stx2/sheA/eae and stx2/stx2g/sheA were equally distributed in 9.6% of total isolates. Phylogenetic typing revealed that pathogenic phylogroups B2 and D were detected among clinical isolates only. Forty-six different patterns were detected by ERIC genotyping. MLST resolved three sequence types of ST70, ST120 and ST394. The heat map showed that 21 isolates were of 70% similarity, 9 groups were of 100% clonality. Conclusions The prevalence of non-O157 EHEC pathotype was marginally higher among the food isolates compared to the clinical ones. The endemic ST120 was detected in cheese, necessitating crucial measures to prevent the spread of this clone. Clinical EHEC isolates exhibited a higher score, and combination of virulence genes compared to food and sewage water isolates, thereby posing a significant public health concern.
  • An Optimized Protocol for the Expression of a Recombinant Protein Fragment of Human Factor B Used For the Production of Polyclonal Antibodies
    Enas Y. Sultan, Dina E. Rizk, Ramadan Hassan, Hany I. Kenawy
    Egyptian Journal of Medical Microbiology Egypt, 2024
    Background: Complement is a critical component of the innate immunity bridging both arms of the immune system. Extensive trials are ongoing to develop therapeutics that target complement and hence alleviating complement-mediated disorders. Objectives: Here, we detail our attempts to express a recombinant protein fragment of human factor B (FBfr) in a high yield and solubility in addition to the production of polyclonal antibody against it. Methodology: We tested different expression conditions and host strains to express FBfr. Moreover, we used the expressed Fbfr to immunize rabbits for production of polyclonal antibody and tested its inhibitory properties against the alternative pathway by in vitro assays. Results: We successfully expressed an alternative complement pathway inhibitor FBfr in E. coli using an optimized protocol. The protein was antigenic and led to the production of a high titer of antibodies in sera of the immunized rabbits. The polyclonal antibody was shown to have a moderate inhibition of the alternative complement pathway activity. Conclusion: optimization of expression parameters can greatly improve the expression levels of recombinant proteins. These proteins can have a functional activity and be used for successful immunization process.
  • Prevalence and antimicrobial susceptibility of Enterobacteriaceae isolated from ready-to-eat foods retailed in Damietta, Egypt
    Samar M. Elsherbeny, Dina E. Rizk, Maha Al-Ashmawy, Rasha Barwa
    Egyptian Journal of Basic and Applied Sciences, 2024
    The presence of Enterobacteria in food in large numbers indicates fecal contamination.This study was carried out to determine the susceptibility of Enterobacteriaceae strains isolated from ready-to-eat foods from the Damietta market.A total of 40 random ready-to-eat food samples were collected from different localities at Damietta Governorate, Egypt.These samples were examined for Enterobacteriaceae count, and identification of members by conventional biochemical methods and antimicrobial susceptibility was determined by disc diffusion method.Enterobacteriaceae isolates were detected among 34/ 40 (85%) examined Ready-to-eat food samples.A total of 139 isolates were identified.The most frequent genus was Klebsiella followed by Enterobacter.Other members such as Citrobacter spp., Proteus spp., Providencia spp., and E. coli spp.were also identified.The isolates showed different susceptibility to 12 tested antimicrobials.Most of the tested isolates showed high resistance percentages to cefepime and ampicillin.Imipenem, ciprofloxacin, amikacin, gentamicin, and cefotaxime were the most effective antimicrobials.In addition, 29% of the isolates were multiple-drug resistant (MDR).The high prevalence of members of Enterobacteriaceae in different food sources is a reliable index of food contamination.So, it is recommended to reconsider restrictions on hygiene and sanitary control in the current food acts and regulations.
  • Prevalence of E. coli Pathotypes: A Comparative Study between Clinical and Environmental Isolates
    Omnia Bahgat, Dina Rizk, Hany Kenawy, Rasha Barwa
    Egyptian Journal of Medical Microbiology Egypt, 2023
    Background: The pervasive species of Escherichia coli range from avirulent to extremely pathogenic strains. Pathogenic strains are a serious public health concern globally, causing gastrointestinal infections or disseminate throughout the body, causing urinary tract infections, and sepsis/meningitis. Among bacterial etiologic agents of gastrointestinal infections, diarrheagenic Escherichia coli (DEC) is the predominant cause of severe diarrhea. Objective: This study aims at determining the prevalence of Escherichia coli pathotypes and serotypes among clinical and environmental isolates. Methodology: A total of 105 presumptive isolates of E. coli were obtained from different clinical (118) and environmental (217) specimens. Confirmed E. coli isolates were subjected to serological identification, as well as determination of pathotypes. Statistical data analysis was performed applying Fisher’s exact test. Results: Of the 335 presumptive specimens, 31.3% (105/335) were confirmed as E. coli. Seropathotyping of the confirmed isolates showed their distribution as 49.5% EHEC, 26.7% EPEC, 18.1% ETEC, and 5.7% EIEC. Alarmingly, high rate of EHEC and ETEC were observed among dairy and meat products (50% and 20%, respectively), while a low rate belonged to EIEC pathotype. Concerning E. coli clinical isolates, EHEC followed by EPEC were the most prevalent pathotypes. Regarding serotypes distribution, the most prevalent serotype among environmental isolates was O26: H11, whereas the most common serotype among clinical isolates was O128: H2. Serotypes O26: H11 and O125: H21 were significantly more prevalent among environmental isolates than clinical isolates, while serotypes O126: H21, O55: H7, O119: H6, and O128: H2 were significantly more prevalent among clinical isolates. Conclusion: This research emphasizes the issue of pathogenic pathotypes becoming progressively prevalent in Egypt. We concluded that pathogenic E. coli has been detected not solely in hospitals, but also in food and dairy products rendering them to be possible reservoirs and vehicles for this pathogen.
  • Chemical compositions and antimicrobial activities of three lemon odor essential oils of Cupressus macrocarpa, Cymbopogon citratus. and Citrus limon
    Asmaa E. Sherif, Yhiya Amen, Dina E. Rizk, Gehad Abdelwahab
    Egyptian Journal of Basic and Applied Sciences, 2023
    Infectious diseases caused by bacteria, fungi and viruses are globally of major concern. Plant essential oils produced by various differentiated structures possess varied properties as significant antiseptic, antibacterial, antifungal, antiviral, antioxidant, anti-parasitic and insecticidal activities. The essential oil of three lemon odor plants; Cupressus macrocarpa, Cymbopogon citratus and Citrus limon were isolated and evaluated for their antimicrobial activities. The analysis of essential oil components of C. macrocarpa by GC-MS revealed D-limonene (38.00%) as the major component followed by citral (9.72%), carveol (6.86%) and citronellal (5.35%). Analyses of the essential oil of C. citratus resulted in the identification of pseudolimonene (19.2%) as the most abundant component followed by D-limonene (12.34%), Ɣ-terpinene (10.89%), citronellol (9.58%), sabinene hydrate (9.24%), (+)-2-bornanone (8.29%) and α-terpinolene (5.53%). Also, GC-MS detected neral dimethyl acetal (41.56%) was the main component of C. limon EO with carveol (12.39%) and citral (11.21%). A study of the antimicrobial activity of the three essential oils against E. coli, Proteus mirabilis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus. and Candida albicans has been evaluated and revealed that C. citratus EO had antibacterial activity against gram-positive organisms and C. limon EO exhibited the strongest antifungal activity against Candida albicans.
  • Incidence of virulence determinants among Streptococcus agalactiae isolated from pregnant women and association with their serotypes
    Reham R. EL-Lakany, Eman S. Abdelmaged, Maher Shams, Ramadan Hassan, Dina E. Rizk
    Egyptian Journal of Basic and Applied Sciences, 2023
    Colonization of pregnant women by Streptococcus agalactiae (S. agalactiae) is the main reason for intrauterine infection or transmission during parturition. This study investigated the prevalence, antimicrobial susceptibility, serotypes, virulence traits of S. agalactiae in pregnant women across three hospitals in Dakahlia Governorate, Egypt. Isolates were identified by culturing onto Hichrome Sterp B selective medium, latex agglutination and biochemical tests. The antimicrobial susceptibility was determined by Kirby-Bauer disc diffusion method. Capsular serotypes were determined by latex agglutination test. Hemolysin production and biofilm formation were assessed quantitively. Virulence genes were detected by polymerase chain reaction (PCR). Among 290 samples, 31.7% were S. agalactiae. All isolates were sensitive to penicillin G, ampicillin, cefotaxime, and vancomycin, but notably resistant to tetracycline (83.6%), erythromycin (68.4%), and clindamycin (54.3%). Serotype III (26.08%) was most common. Strong biofilm was produced by 72.8% of isolates. High hemolytic activity was seen (80.4%). Prevalent virulence genes included fsbA (97.8%), fbsB (96.7%), and scpB (96.7%). rib and alp4 were frequent surface protein genes (60.8% and 57.6%, respectively). Serotypes were significantly correlated to clindamycin resistance, biofilm production (Ia, III, VI serotypes), hemolytic activity (serotype V), and several virulence genes and surface proteins. Our study found a high prevalence of S. agalactiae isolates in pregnant women with diverse resistance patterns, high virulence indicating potential for increased pathogenicity.
  • Inhibition of the lectin pathway of complement activation reduces LPS-induced acute respiratory distress syndrome in mice
    Youssif M. Ali, Nicholas J. Lynch, Ahmed A. Shaaban, Dina E. Rizk, Shaymaa H. Abdel-Rahman, Priyanka Khatri, Munehisa Yabuki, Sadam Yaseen, Thomas Dudler, Gregory Demopulos, Wilhelm J. Schwaeble
    Frontiers in Immunology, 2023
    Acute respiratory distress syndrome (ARDS) is a life-threatening disorder with a high rate of mortality. Complement activation in ARDS initiates a robust inflammatory reaction that can cause progressive endothelial injury in the lung. Here, we tested whether inhibition of the lectin pathway of complement could reduce the pathology and improve the outcomes in a murine model of LPS-induced lung injury that closely mimics ARDS in human. In vitro, LPS binds to murine and human collectin 11, human MBL and murine MBL-A, but not to C1q, the recognition subcomponent of the classical pathway. This binding initiates deposition of the complement activation products C3b, C4b and C5b-9 on LPS via the lectin pathway. HG-4, a monoclonal antibody that targets MASP-2, a key enzyme in the lectin pathway, inhibited lectin pathway functional activity in vitro, with an IC50 of circa 10nM. Administration of HG4 (5mg/kg) in mice led to almost complete inhibition of the lectin pathway activation for 48hrs, and 50% inhibition at 60hrs post administration. Inhibition of the lectin pathway in mice prior to LPS-induced lung injury improved all pathological markers tested. HG4 reduces the protein concentration in bronchoalveolar lavage fluid (p<0.0001) and levels of myeloid peroxide (p<0.0001), LDH (p<0.0001), TNFα and IL6 (both p<0.0001). Lung injury was significantly reduced (p<0.001) and the survival time of the mice increased (p<0.01). From the previous findings we concluded that inhibition of the lectin pathway has the potential to prevent ARDS pathology.
  • Prevalence of Vancomycin Resistance among Clinical Isolates of MRSA from Different Governorates in Egypt
    Wesam Ibrahiem, Dina Rizk, Hany Kenawy, Ramadan Hassan
    Egyptian Journal of Medical Microbiology Egypt, 2022
    Background : The uncontrolled use of vancomycin led to an upsurge of vancomycin-resistant S. aureus (VRSA) throughout the world. Objective : The goal of this study is to screen vancomycin resistance among MRSA isolates, determine antimicrobial resistance pattern and evaluate the distribution of virulence genes among these isolates. Methodology : A total of 127 S. aureus clinical isolates were used, MRSA isolates were identified and antimicrobial sensitivity pattern for nine antimicrobial agents from different classes was assessed. In addition, vancomycin MIC was determined by standard agar dilution method and PCR identification of vancomycin resistance encoding genes vanA and vanB was performed. Moreover, the prevalence of eight different virulence genes was determined among different vancomycin resistance categories. Results : All isolates were identified phenotypically as MRSA. However, mecA gene was detected only in 95.28% of isolates. The highest and lowest percentage of resistance was recorded for clindamycin (82.68%) and trimethoprim (11.81%), respectively. Vancomycin resistance level was 23.62% of isolates, while vanA and vanB genes were detected only in 16.67% and 10% of VRSA isolates, respectively. The highest prevalence of virulence genes was found for icaA, followed by hld, hlb, icaD, hlg, hla, tsst and cna, respectively in the tested isolates. In addition, VRSA isolates showed higher mean virulence score (MVS) of 3.6 compared to VISA and VSSA isolates. Conclusion : This study highlights the alarming problem of the increasing incidence of VRSA infections in Egypt. Therefore, there is an urgent need to rationalize vancomycin consumption and to continuously monitor the prevalence of VRSA strains.
  • A small fragment of factor B as a potential inhibitor of complement alternative pathway activity
    Enas Yasser Sultan, Dina Eid Rizk, Hany Ibrahim Kenawy, Ramadan Hassan
    Immunobiology, 2021
  • Comparative assessment of different PCR-based typing methods of pseudomonas aeruginosa isolates
    Shaymaa H Abdel-Rhman, Dina E Rizk
    Infection and Drug Resistance, 2021
    Introduction Pseudomonas aeruginosa is one of the important causes of nosocomial infections. Analyzing the diversity of these isolates is important to control the diseases caused by them. Studies of molecular epidemiology depend on the application of typing methods. Purpose This study aims to assess the performance of PCR- based typing techniques (RAPD, ribotyping, tDNA, and ERIC) in determining the genetic diversity of 44 P. aeruginosa urinary isolates. Methods Performance parameters were analyzed for each of the tested methods. The banding pattern was assessed by calculating polymorphism, genotypic gene diversity and the effective multiplex ratio. Moreover, strain diversity, typeability, and discriminatory power were used to measure the efficiency of typing methods. The congruence among typing methods was calculated by Rand’s and Wallace coefficients. Results P-640 among RAPD primers and Ribo-2 among ribotyping primers were more informative as they gave high strain diversity, the highest number of clusters, and highest discriminatory power (ISD=70.45%, 29 clusters at 70% cutoff, DI=0.97 and ISD=75%, 25 clusters at 70% cutoff DI=0.969, respectively). Comparison of typing methods showed that RAPD-PCR gave the highest mean percent polymorphism per assay (76.85%) followed by ERIC-PCR. ERIC-PCR outperformed in most marker parameters; highest mean number of alleles, number of monomorphic bands per assay unit, mean genotypic gene diversity, effective multiplex ratio, and assay efficiency index. Calculated congruence revealed that individual methods demonstrate moderate to poor predictive power. Interestingly, this power increased by combining data obtained from another method. Conclusion RAPD primer (P-640) had more discrimination power followed by ribo-2 and ERIC. The performance and predictive power of typing methods can be improved by combining data obtained from different methods as ERIC+OPA-02 and ERIC+P-640 combinations gave complete typeability and discrimination of isolates. ERIC, ERIC+OPA-02, and ERIC+P-640 combinations can provide finer discrimination and classification of P. aeruginosa strains than the other tested methods.
  • Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt
    Shaymaa H. Abdel-Rhman, Rehab M. Elbargisy, Dina E. Rizk
    International Journal of Microbiology, 2021
  • Seroepidemiology, Antimicrobial Susceptibility and Virulence Characteristics of Clinical Klebsiella pneumoniae Isolates in Mansoura University Hospitals
    Aya H. Elasmer, Mohammed Y. Ibrahim, Dina E. Rizk
    Egyptian Journal of Medical Microbiology Egypt, 2021
  • Effect of sub-minimum inhibitory concentrations of tyrosol and edta on quorum sensing and virulence of pseudomonas aeruginosa
    Shaymaa H Abdel-Rhman, Dina E Rizk, Eman S Abdelmegeed
    Infection and Drug Resistance, 2020
  • Prevalence and characterization of streptococcus pyogenes clinical isolates from different hospitals and clinics in Mansoura
    Zeinab M. Helal, Dina E. Rizk, Mohamed M. Adel El-Sokkary, Ramadan Hassan
    International Journal of Microbiology, 2020
  • Virulence characteristics and molecular relatedness of methicillin resistant Staphylococcus aureus harboring different staphylococcal cassette chromosome mec
    Rana El-baz, Dina E. Rizk, Rasha Barwa, Ramadan Hassan
    Microbial Pathogenesis, 2017
  • Emergence of class 1 to 3 integrons among members of Enterobacteriaceae in Egypt
    Dina E. Rizk, Areej M. El-Mahdy
    Microbial Pathogenesis, 2017